Restriction map for
pF1KB9025
(Linear) (Six-base) MAPSORT of: KIBB9025.seq Check: 205 from: 1 to: 1302
KIBB9025.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 626 1302
Size: 626 676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 154 588 1117 1181 1302
Size: 88 66 434 529 64 121
Fragments arranged by size:
529 434 121 88 66 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 455 1302
Size: 455 847
ApoI r'AATT_y
Cuts at: 0 106 354 415 465 590 731 829 1302
Size: 106 248 61 50 125 141 98 473
Fragments arranged by size:
473 248 141 125 106 98 61 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1084 1117 1302
Size: 1084 33 185
Fragments arranged by size:
1084 185 33
BanI G'GyrC_C
Cuts at: 0 8 1302
Size: 8 1294
BanII G_rGCy'C
Cuts at: 0 1218 1302
Size: 1218 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 1302
Size: 405 897
BbvCI CC'TCA_GC
Cuts at: 0 4 407 1302
Size: 4 403 895
Fragments arranged by size:
895 403 4
BglII A'GATC_T
Cuts at: 0 453 1302
Size: 453 849
Bpu10I CC'TnA_GC
Cuts at: 0 4 407 1302
Size: 4 403 895
Fragments arranged by size:
895 403 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 1302
Size: 179 30 1093
Fragments arranged by size:
1093 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 203 326 967 1302
Size: 203 123 641 335
Fragments arranged by size:
641 335 203 123
BseYI C'CCAG_C
Cuts at: 0 286 1302
Size: 286 1016
BsiHKAI G_wGCw'C
Cuts at: 0 1115 1302
Size: 1115 187
Bsp1286I G_dGCh'C
Cuts at: 0 1115 1218 1302
Size: 1115 103 84
Fragments arranged by size:
1115 103 84
BspHI T'CATG_A
Cuts at: 0 345 1302
Size: 345 957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 816 1302
Size: 816 486
BssSI C'ACGA_G
Cuts at: 0 1079 1302
Size: 1079 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 676 1302
Size: 676 626
BstEII G'GTnAC_C
Cuts at: 0 637 742 1255 1302
Size: 637 105 513 47
Fragments arranged by size:
637 513 105 47
BstYI r'GATC_y
Cuts at: 0 453 1302
Size: 453 849
Bsu36I CC'TnA_GG
Cuts at: 0 224 1302
Size: 224 1078
BtsI GCAGTG_nn'
Cuts at: 0 200 245 1144 1302
Size: 200 45 899 158
Fragments arranged by size:
899 200 158 45
DraI TTT'AAA
Cuts at: 0 527 1302
Size: 527 775
DrdI GACnn_nn'nnGTC
Cuts at: 0 137 1302
Size: 137 1165
EaeI y'GGCC_r
Cuts at: 0 1167 1302
Size: 1167 135
EarI CTCTTCn'nnn_
Cuts at: 0 130 1302
Size: 130 1172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 154 588 1117 1181 1302
Size: 88 66 434 529 64 121
Fragments arranged by size:
529 434 121 88 66 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 797 1302
Size: 797 505
EcoRI G'AATT_C
Cuts at: 0 106 1302
Size: 106 1196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1294 1302
Size: 1294 8
MfeI C'AATT_G
Cuts at: 0 665 1302
Size: 665 637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1302
Size: 531 771
MscI TGG'CCA
Cuts at: 0 1169 1302
Size: 1169 133
NruI TCG'CGA
Cuts at: 0 946 1302
Size: 946 356
NspI r_CATG'y
Cuts at: 0 406 559 1302
Size: 406 153 743
Fragments arranged by size:
743 406 153
PacI TTA_AT'TAA
Cuts at: 0 1001 1302
Size: 1001 301
SexAI A'CCwGG_T
Cuts at: 0 633 1302
Size: 633 669
StyI C'CwwG_G
Cuts at: 0 963 1104 1302
Size: 963 141 198
Fragments arranged by size:
963 198 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 705 1302
Size: 705 597
TatI w'GTAC_w
Cuts at: 0 903 1302
Size: 903 399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1236 1268 1302
Size: 179 32 1025 32 34
Fragments arranged by size:
1025 179 34 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 838 1302
Size: 838 464
Enzymes that do cut:
AccI AcuI AlfI ApoI BaeI BanI BanII BbsI
BbvCI BglII Bpu10I BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspHI BspMI BssSI BstAPI BstEII BstYI Bsu36I BtsI
DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI FalI
MfeI MmeI MscI NruI NspI PacI SexAI StyI
TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BstBI BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI