Restriction map for
pF1KB9075
(Linear) (Six-base) MAPSORT of: KIBB9075.seq Check: 2873 from: 1 to: 4014
KIBB9075.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1014 4014
Size: 1014 3000
AccI GT'mk_AC
Cuts at: 0 396 1922 4014
Size: 396 1526 2092
Fragments arranged by size:
2092 1526 396
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1208 3622 3784 4014
Size: 467 741 2414 162 230
Fragments arranged by size:
2414 741 467 230 162
AfeI AGC'GCT
Cuts at: 0 3769 4014
Size: 3769 245
AflIII A'CryG_T
Cuts at: 0 3242 4014
Size: 3242 772
AhdI GACnn_n'nnGTC
Cuts at: 0 3246 4014
Size: 3246 768
AleI CACnn'nnGTG
Cuts at: 0 973 1660 2296 2559 4014
Size: 973 687 636 263 1455
Fragments arranged by size:
1455 973 687 636 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2507 2539 4014
Size: 2507 32 1475
Fragments arranged by size:
2507 1475 32
AlwNI CAG_nnn'CTG
Cuts at: 0 11 144 364 3875 4014
Size: 11 133 220 3511 139
Fragments arranged by size:
3511 220 139 133 11
ApoI r'AATT_y
Cuts at: 0 1583 4014
Size: 1583 2431
AvaI C'yCGr_G
Cuts at: 0 2062 2468 3131 4014
Size: 2062 406 663 883
Fragments arranged by size:
2062 883 663 406
BanI G'GyrC_C
Cuts at: 0 112 1403 1894 4014
Size: 112 1291 491 2120
Fragments arranged by size:
2120 1291 491 112
BanII G_rGCy'C
Cuts at: 0 461 2215 2905 2978 3363 4014
Size: 461 1754 690 73 385 651
Fragments arranged by size:
1754 690 651 461 385 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 2348 3032 4014
Size: 2348 684 982
Fragments arranged by size:
2348 982 684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1070 1104 2878 2912 4014
Size: 1070 34 1774 34 1102
Fragments arranged by size:
1774 1102 1070 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1384 4014
Size: 1384 2630
BclI T'GATC_A
Cuts at: 0 2004 2276 4014
Size: 2004 272 1738
Fragments arranged by size:
2004 1738 272
BglII A'GATC_T
Cuts at: 0 2991 4014
Size: 2991 1023
BlpI GC'TnA_GC
Cuts at: 0 43 220 1219 3935 4014
Size: 43 177 999 2716 79
Fragments arranged by size:
2716 999 177 79 43
Bme1580I G_kGCm'C
Cuts at: 0 3896 4014
Size: 3896 118
BmgBI CAC'GTC
Cuts at: 0 3243 3897 4014
Size: 3243 654 117
Fragments arranged by size:
3243 654 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 709 1345 2494 3863 4014
Size: 21 688 636 1149 1369 151
Fragments arranged by size:
1369 1149 688 636 151 21
BmtI G_CTAG'C
Cuts at: 0 1559 4014
Size: 1559 2455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1532 4014
Size: 1532 2482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1927 2290 4014
Size: 816 1111 363 1724
Fragments arranged by size:
1724 1111 816 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 2746 3790 3837 4014
Size: 275 2471 1044 47 177
Fragments arranged by size:
2471 1044 275 177 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 3296 3410 4014
Size: 3296 114 604
Fragments arranged by size:
3296 604 114
BsaAI yAC'GTr
Cuts at: 0 2561 4014
Size: 2561 1453
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 134 164 1970 2000 3022 3052 4014
Size: 134 30 1806 30 1022 30 962
Fragments arranged by size:
1806 1022 962 134 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2962 4014
Size: 2962 1052
BseYI C'CCAG_C
Cuts at: 0 116 2682 4014
Size: 116 2566 1332
Fragments arranged by size:
2566 1332 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2676 4014
Size: 2676 1338
BsiHKAI G_wGCw'C
Cuts at: 0 461 1844 2905 3363 4014
Size: 461 1383 1061 458 651
Fragments arranged by size:
1383 1061 651 461 458
BsmI GAATG_Cn'
Cuts at: 0 994 3053 3705 4014
Size: 994 2059 652 309
Fragments arranged by size:
2059 994 652 309
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1748 4014
Size: 1748 2266
Bsp1286I G_dGCh'C
Cuts at: 0 461 1844 2215 2905 2978 3363 3896 4014
Size: 461 1383 371 690 73 385 533 118
Fragments arranged by size:
1383 690 533 461 385 371 118 73
BspHI T'CATG_A
Cuts at: 0 1868 3656 4014
Size: 1868 1788 358
Fragments arranged by size:
1868 1788 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1014 1131 2687 3905 4014
Size: 1014 117 1556 1218 109
Fragments arranged by size:
1556 1218 1014 117 109
BsrBI CCG'CTC
Cuts at: 0 2433 4014
Size: 2433 1581
BsrFI r'CCGG_y
Cuts at: 0 1058 4014
Size: 1058 2956
BsrGI T'GTAC_A
Cuts at: 0 413 3483 4014
Size: 413 3070 531
Fragments arranged by size:
3070 531 413
BssHII G'CGCG_C
Cuts at: 0 35 4014
Size: 35 3979
BssSI C'ACGA_G
Cuts at: 0 777 1154 4014
Size: 777 377 2860
Fragments arranged by size:
2860 777 377
BstAPI GCAn_nnn'nTGC
Cuts at: 0 126 4014
Size: 126 3888
BstEII G'GTnAC_C
Cuts at: 0 1118 4014
Size: 1118 2896
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2985 3027 4014
Size: 2985 42 987
Fragments arranged by size:
2985 987 42
BstYI r'GATC_y
Cuts at: 0 64 90 2991 4014
Size: 64 26 2901 1023
Fragments arranged by size:
2901 1023 64 26
BstZ17I GTA'TAC
Cuts at: 0 1923 4014
Size: 1923 2091
Bsu36I CC'TnA_GG
Cuts at: 0 3282 3337 4014
Size: 3282 55 677
Fragments arranged by size:
3282 677 55
BtgI C'CryG_G
Cuts at: 0 2600 4014
Size: 2600 1414
BtsI GCAGTG_nn'
Cuts at: 0 142 1253 2187 2431 4014
Size: 142 1111 934 244 1583
Fragments arranged by size:
1583 1111 934 244 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1248 1283 4014
Size: 1248 35 2731
Fragments arranged by size:
2731 1248 35
DraI TTT'AAA
Cuts at: 0 107 506 1245 2085 4014
Size: 107 399 739 840 1929
Fragments arranged by size:
1929 840 739 399 107
DraIII CAC_nnn'GTG
Cuts at: 0 2013 4014
Size: 2013 2001
DrdI GACnn_nn'nnGTC
Cuts at: 0 22 4014
Size: 22 3992
EaeI y'GGCC_r
Cuts at: 0 1826 2015 2603 2655 3017 4014
Size: 1826 189 588 52 362 997
Fragments arranged by size:
1826 997 588 362 189 52
EarI CTCTTCn'nnn_
Cuts at: 0 1184 1454 2162 2317 2496 2939 4014
Size: 1184 270 708 155 179 443 1075
Fragments arranged by size:
1184 1075 708 443 270 179 155
EcoICRI GAG'CTC
Cuts at: 0 459 2903 3361 4014
Size: 459 2444 458 653
Fragments arranged by size:
2444 653 459 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 816 1208 1927 2290 3622 3784 4014
Size: 467 349 392 719 363 1332 162 230
Fragments arranged by size:
1332 719 467 392 363 349 230 162
EcoO109I rG'GnC_Cy
Cuts at: 0 24 1713 2707 3063 4014
Size: 24 1689 994 356 951
Fragments arranged by size:
1689 994 951 356 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2375 2525 3831 4014
Size: 2375 150 1306 183
Fragments arranged by size:
2375 1306 183 150
HaeII r_GCGC'y
Cuts at: 0 3771 4014
Size: 3771 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 642 674 2368 2400 3022 3054
Size: 132 32 478 32 1694 32 622 32
Cuts at: 3054 3232 3264 3345 3377 3967 3999 4014
Size: 178 32 81 32 590 32 15
Fragments arranged by size:
1694 622 590 478 178 132 81 32
32 32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 966 1683 1737 2676 4014
Size: 966 717 54 939 1338
Fragments arranged by size:
1338 966 939 717 54
HindIII A'AGCT_T
Cuts at: 0 1644 2892 4014
Size: 1644 1248 1122
Fragments arranged by size:
1644 1248 1122
HpaI GTT'AAC
Cuts at: 0 966 1683 1737 2676 4014
Size: 966 717 54 939 1338
Fragments arranged by size:
1338 966 939 717 54
MfeI C'AATT_G
Cuts at: 0 750 4014
Size: 750 3264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2777 4014
Size: 2777 1237
MscI TGG'CCA
Cuts at: 0 1828 2017 2657 3019 4014
Size: 1828 189 640 362 995
Fragments arranged by size:
1828 995 640 362 189
MslI CAynn'nnrTG
Cuts at: 0 973 983 1003 1660 2051 2296 2559 2983
Size: 973 10 20 657 391 245 263 424
Cuts at: 2983 3352 3709 4014
Size: 369 357 305
Fragments arranged by size:
973 657 424 391 369 357 305 263
245 20 10
MspA1I CmG'CkG
Cuts at: 0 47 317 3887 4014
Size: 47 270 3570 127
Fragments arranged by size:
3570 270 127 47
NdeI CA'TA_TG
Cuts at: 0 1368 4014
Size: 1368 2646
NheI G'CTAG_C
Cuts at: 0 1555 4014
Size: 1555 2459
NspI r_CATG'y
Cuts at: 0 2291 4014
Size: 2291 1723
PflMI CCAn_nnn'nTGG
Cuts at: 0 2456 4014
Size: 2456 1558
PfoI T'CCnGG_A
Cuts at: 0 1472 4014
Size: 1472 2542
PpuMI rG'GwC_Cy
Cuts at: 0 24 1713 3063 4014
Size: 24 1689 1350 951
Fragments arranged by size:
1689 1350 951 24
PsiI TTA'TAA
Cuts at: 0 2392 3143 4014
Size: 2392 751 871
Fragments arranged by size:
2392 871 751
PstI C_TGCA'G
Cuts at: 0 268 1909 4014
Size: 268 1641 2105
Fragments arranged by size:
2105 1641 268
PvuII CAG'CTG
Cuts at: 0 47 317 3887 4014
Size: 47 270 3570 127
Fragments arranged by size:
3570 270 127 47
SacI G_AGCT'C
Cuts at: 0 461 2905 3363 4014
Size: 461 2444 458 651
Fragments arranged by size:
2444 651 461 458
ScaI AGT'ACT
Cuts at: 0 270 3370 4014
Size: 270 3100 644
Fragments arranged by size:
3100 644 270
SfcI C'TryA_G
Cuts at: 0 264 312 876 1803 1905 3225 4014
Size: 264 48 564 927 102 1320 789
Fragments arranged by size:
1320 927 789 564 264 102 48
SmaI CCC'GGG
Cuts at: 0 2470 3133 4014
Size: 2470 663 881
Fragments arranged by size:
2470 881 663
SmlI C'TyrA_G
Cuts at: 0 290 2761 3805 3816 4014
Size: 290 2471 1044 11 198
Fragments arranged by size:
2471 1044 290 198 11
StuI AGG'CCT
Cuts at: 0 2843 3799 4014
Size: 2843 956 215
Fragments arranged by size:
2843 956 215
StyI C'CwwG_G
Cuts at: 0 1562 3794 4014
Size: 1562 2232 220
Fragments arranged by size:
2232 1562 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 4014
Size: 130 3884
TatI w'GTAC_w
Cuts at: 0 268 413 754 2612 3306 3368 3483 3995
Size: 268 145 341 1858 694 62 115 512
Cuts at: 3995 4014
Size: 19
Fragments arranged by size:
1858 694 512 341 268 145 115 62
19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 134 166 1970 2002 2410 2442 4014
Size: 134 32 1804 32 408 32 1572
Fragments arranged by size:
1804 1572 408 134 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1579 4014
Size: 1579 2435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3886 4014
Size: 3886 128
XmaI C'CCGG_G
Cuts at: 0 2468 3131 4014
Size: 2468 663 883
Fragments arranged by size:
2468 883 663
XmnI GAAnn'nnTTC
Cuts at: 0 3769 4014
Size: 3769 245
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApoI AvaI BanI BanII BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NdeI
NheI NspI PflMI PfoI PpuMI PsiI PstI PvuII
SacI ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BfrBI BglI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiWI
BspEI BsrDI BstBI BtgZI ClaI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XhoI ZraI