Restriction map for
pF1KB9097
(Linear) (Six-base) MAPSORT of: KIBB9097.seq Check: 7331 from: 1 to: 4098
KIBB9097.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 0 1475 3581 4098
Size: 1475 2106 517
Fragments arranged by size:
2106 1475 517
Acc65I G'GTAC_C
Cuts at: 0 1532 4098
Size: 1532 2566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1097 1732 1808 2135 2922 4098
Size: 157 940 635 76 327 787 1176
Fragments arranged by size:
1176 940 787 635 327 157 76
AfeI AGC'GCT
Cuts at: 0 2943 4098
Size: 2943 1155
AflIII A'CryG_T
Cuts at: 0 652 4098
Size: 652 3446
AgeI A'CCGG_T
Cuts at: 0 1529 4098
Size: 1529 2569
AhdI GACnn_n'nnGTC
Cuts at: 0 3582 4098
Size: 3582 516
AleI CACnn'nnGTG
Cuts at: 0 1762 4098
Size: 1762 2336
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1290 2755 4098
Size: 135 1155 1465 1343
Fragments arranged by size:
1465 1343 1155 135
ApaLI G'TGCA_C
Cuts at: 0 669 4098
Size: 669 3429
ApoI r'AATT_y
Cuts at: 0 1373 2169 4098
Size: 1373 796 1929
Fragments arranged by size:
1929 1373 796
AvaI C'yCGr_G
Cuts at: 0 277 410 697 924 2691 2981 3859 4098
Size: 277 133 287 227 1767 290 878 239
Fragments arranged by size:
1767 878 290 287 277 239 227 133
AvrII C'CTAG_G
Cuts at: 0 1224 4098
Size: 1224 2874
BanI G'GyrC_C
Cuts at: 0 1409 1526 1532 2026 4098
Size: 1409 117 6 494 2072
Fragments arranged by size:
2072 1409 494 117 6
BanII G_rGCy'C
Cuts at: 0 47 1139 1691 4098
Size: 47 1092 552 2407
Fragments arranged by size:
2407 1092 552 47
BbeI G_GCGC'C
Cuts at: 0 1413 4098
Size: 1413 2685
BbsI GAAGACnn'nnnn_
Cuts at: 0 586 2859 3552 4079 4098
Size: 586 2273 693 527 19
Fragments arranged by size:
2273 693 586 527 19
BbvCI CC'TCA_GC
Cuts at: 0 856 4098
Size: 856 3242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 144 178 3643 3677 4098
Size: 144 34 3465 34 421
Fragments arranged by size:
3465 421 144 34 34
BclI T'GATC_A
Cuts at: 0 2804 4098
Size: 2804 1294
BglI GCCn_nnn'nGGC
Cuts at: 0 926 3619 4098
Size: 926 2693 479
Fragments arranged by size:
2693 926 479
BglII A'GATC_T
Cuts at: 0 1799 4098
Size: 1799 2299
Bme1580I G_kGCm'C
Cuts at: 0 646 673 922 2198 4098
Size: 646 27 249 1276 1900
Fragments arranged by size:
1900 1276 646 249 27
BmgBI CAC'GTC
Cuts at: 0 1499 1659 1683 4098
Size: 1499 160 24 2415
Fragments arranged by size:
2415 1499 160 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1308 4077 4098
Size: 1308 2769 21
Fragments arranged by size:
2769 1308 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 1473 4098
Size: 939 534 2625
Fragments arranged by size:
2625 939 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1141 2332 3796 3901 4098
Size: 166 975 1191 1464 105 197
Fragments arranged by size:
1464 1191 975 197 166 105
Bpu10I CC'TnA_GC
Cuts at: 0 856 3322 4098
Size: 856 2466 776
Fragments arranged by size:
2466 856 776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 3532 4098
Size: 358 3174 566
Fragments arranged by size:
3174 566 358
BsaI GGTCTCn'nnnn_
Cuts at: 0 284 1194 3278 3392 3831 4098
Size: 284 910 2084 114 439 267
Fragments arranged by size:
2084 910 439 284 267 114
BsaAI yAC'GTr
Cuts at: 0 653 1161 4098
Size: 653 508 2937
Fragments arranged by size:
2937 653 508
BsaHI Gr'CG_yC
Cuts at: 0 1410 1472 3578 4098
Size: 1410 62 2106 520
Fragments arranged by size:
2106 1410 520 62
BsaWI w'CCGG_w
Cuts at: 0 13 1529 3269 3494 4098
Size: 13 1516 1740 225 604
Fragments arranged by size:
1740 1516 604 225 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2305 2335 2834 2864 4098
Size: 2305 30 499 30 1234
Fragments arranged by size:
2305 1234 499 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 32 574 1246 1857 2056 2155 3814 3859
Size: 32 542 672 611 199 99 1659 45
Cuts at: 3859 3883 3915 4098
Size: 24 32 183
Fragments arranged by size:
1659 672 611 542 199 183 99 45
32 32 24
BseYI C'CCAG_C
Cuts at: 0 2436 2895 3192 4098
Size: 2436 459 297 906
Fragments arranged by size:
2436 906 459 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 2161 4098
Size: 138 2023 1937
Fragments arranged by size:
2023 1937 138
BsiEI CG_ry'CG
Cuts at: 0 2276 4098
Size: 2276 1822
BsiHKAI G_wGCw'C
Cuts at: 0 673 4098
Size: 673 3425
Bsp1286I G_dGCh'C
Cuts at: 0 47 646 673 922 1139 1691 2198 4098
Size: 47 599 27 249 217 552 507 1900
Fragments arranged by size:
1900 599 552 507 249 217 47 27
BspEI T'CCGG_A
Cuts at: 0 13 4098
Size: 13 4085
BsrBI CCG'CTC
Cuts at: 0 4065 4098
Size: 4065 33
BsrDI GCAATG_nn'
Cuts at: 0 1106 4098
Size: 1106 2992
BsrFI r'CCGG_y
Cuts at: 0 769 1529 1535 1973 2681 4098
Size: 769 760 6 438 708 1417
Fragments arranged by size:
1417 769 760 708 438 6
BsrGI T'GTAC_A
Cuts at: 0 1805 4098
Size: 1805 2293
BssHII G'CGCG_C
Cuts at: 0 719 4098
Size: 719 3379
BssSI C'ACGA_G
Cuts at: 0 2422 4098
Size: 2422 1676
BstAPI GCAn_nnn'nTGC
Cuts at: 0 959 4098
Size: 959 3139
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1783 1842 2516 3057 4098
Size: 1783 59 674 541 1041
Fragments arranged by size:
1783 1041 674 541 59
BstYI r'GATC_y
Cuts at: 0 1166 1554 1799 2751 4098
Size: 1166 388 245 952 1347
Fragments arranged by size:
1347 1166 952 388 245
Bsu36I CC'TnA_GG
Cuts at: 0 397 1955 4098
Size: 397 1558 2143
Fragments arranged by size:
2143 1558 397
BtgI C'CryG_G
Cuts at: 0 318 554 1760 1782 2270 4098
Size: 318 236 1206 22 488 1828
Fragments arranged by size:
1828 1206 488 318 236 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2793 4098
Size: 2793 1305
BtsI GCAGTG_nn'
Cuts at: 0 3940 4098
Size: 3940 158
ClaI AT'CG_AT
Cuts at: 0 2402 4098
Size: 2402 1696
DraI TTT'AAA
Cuts at: 0 2549 4098
Size: 2549 1549
DraIII CAC_nnn'GTG
Cuts at: 0 3180 4098
Size: 3180 918
EaeI y'GGCC_r
Cuts at: 0 76 2234 2273 2554 3086 3341 3620 4098
Size: 76 2158 39 281 532 255 279 478
Fragments arranged by size:
2158 532 478 281 279 255 76 39
EagI C'GGCC_G
Cuts at: 0 2273 4098
Size: 2273 1825
EarI CTCTTCn'nnn_
Cuts at: 0 301 337 1875 2300 4098
Size: 301 36 1538 425 1798
Fragments arranged by size:
1798 1538 425 301 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 48 1159 4098
Size: 48 1111 2939
Fragments arranged by size:
2939 1111 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 166 1097 1141 1732 1808 2135 2332
Size: 157 9 931 44 591 76 327 197
Cuts at: 2332 2922 3796 3901 4098
Size: 590 874 105 197
Fragments arranged by size:
931 874 591 590 327 197 197 157
105 76 44 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 971 1228 4098
Size: 971 257 2870
Fragments arranged by size:
2870 971 257
EcoO109I rG'GnC_Cy
Cuts at: 0 23 537 976 2089 4098
Size: 23 514 439 1113 2009
Fragments arranged by size:
2009 1113 514 439 23
EcoRV GAT'ATC
Cuts at: 0 2406 3474 4098
Size: 2406 1068 624
Fragments arranged by size:
2406 1068 624
FspI TGC'GCA
Cuts at: 0 3733 4098
Size: 3733 365
FspAI rTGC'GCAy
Cuts at: 0 3733 4098
Size: 3733 365
HaeII r_GCGC'y
Cuts at: 0 144 825 863 1413 2450 2945 4098
Size: 144 681 38 550 1037 495 1153
Fragments arranged by size:
1153 1037 681 550 495 144 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 475 507 1046 1078 2796 2828
Size: 354 32 89 32 539 32 1718 32
Cuts at: 2828 3568 3600 3940 3972 4098
Size: 740 32 340 32 126
Fragments arranged by size:
1718 740 539 354 340 126 89 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 3146 4098
Size: 3146 952
KasI G'GCGC_C
Cuts at: 0 1409 4098
Size: 1409 2689
KpnI G_GTAC'C
Cuts at: 0 1536 4098
Size: 1536 2562
MfeI C'AATT_G
Cuts at: 0 3168 4098
Size: 3168 930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1116 1661 2477 3671 4098
Size: 1116 545 816 1194 427
Fragments arranged by size:
1194 1116 816 545 427
MscI TGG'CCA
Cuts at: 0 2236 3088 4098
Size: 2236 852 1010
Fragments arranged by size:
2236 1010 852
MslI CAynn'nnrTG
Cuts at: 0 1144 1178 1762 1781 2191 2230 2245 4098
Size: 1144 34 584 19 410 39 15 1853
Fragments arranged by size:
1853 1144 584 410 39 34 19 15
MspA1I CmG'CkG
Cuts at: 0 135 320 611 629 713 1643 1947 2414
Size: 135 185 291 18 84 930 304 467
Cuts at: 2414 4077 4098
Size: 1663 21
Fragments arranged by size:
1663 930 467 304 291 185 135 84
21 18
NarI GG'CG_CC
Cuts at: 0 1410 4098
Size: 1410 2688
NcoI C'CATG_G
Cuts at: 0 554 1760 4098
Size: 554 1206 2338
Fragments arranged by size:
2338 1206 554
NspI r_CATG'y
Cuts at: 0 912 957 3120 3717 3738 4098
Size: 912 45 2163 597 21 360
Fragments arranged by size:
2163 912 597 360 45 21
PasI CC'CwG_GG
Cuts at: 0 3988 4098
Size: 3988 110
PflMI CCAn_nnn'nTGG
Cuts at: 0 1643 4098
Size: 1643 2455
PfoI T'CCnGG_A
Cuts at: 0 479 3773 3818 4098
Size: 479 3294 45 280
Fragments arranged by size:
3294 479 280 45
PmlI CAC'GTG
Cuts at: 0 653 4098
Size: 653 3445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1885 1917 4098
Size: 1885 32 2181
Fragments arranged by size:
2181 1885 32
PpuMI rG'GwC_Cy
Cuts at: 0 23 537 976 4098
Size: 23 514 439 3122
Fragments arranged by size:
3122 514 439 23
PshAI GACnn'nnGTC
Cuts at: 0 542 3704 4098
Size: 542 3162 394
Fragments arranged by size:
3162 542 394
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2917 2949 4098
Size: 2917 32 1149
Fragments arranged by size:
2917 1149 32
PstI C_TGCA'G
Cuts at: 0 718 2446 4098
Size: 718 1728 1652
Fragments arranged by size:
1728 1652 718
PvuII CAG'CTG
Cuts at: 0 135 713 1643 2414 4098
Size: 135 578 930 771 1684
Fragments arranged by size:
1684 930 771 578 135
SacII CC_GC'GG
Cuts at: 0 321 4098
Size: 321 3777
SanDI GG'GwC_CC
Cuts at: 0 23 4098
Size: 23 4075
SapI GCTCTTCn'nnn_
Cuts at: 0 2300 4098
Size: 2300 1798
ScaI AGT'ACT
Cuts at: 0 250 2935 4098
Size: 250 2685 1163
Fragments arranged by size:
2685 1163 250
SfcI C'TryA_G
Cuts at: 0 714 804 1548 2442 3837 4098
Size: 714 90 744 894 1395 261
Fragments arranged by size:
1395 894 744 714 261 90
SfoI GGC'GCC
Cuts at: 0 1411 4098
Size: 1411 2687
SmaI CCC'GGG
Cuts at: 0 2693 4098
Size: 2693 1405
SmlI C'TyrA_G
Cuts at: 0 277 373 3547 4098
Size: 277 96 3174 551
Fragments arranged by size:
3174 551 277 96
SphI G_CATG'C
Cuts at: 0 912 3120 4098
Size: 912 2208 978
Fragments arranged by size:
2208 978 912
StuI AGG'CCT
Cuts at: 0 2529 3765 4098
Size: 2529 1236 333
Fragments arranged by size:
2529 1236 333
StyI C'CwwG_G
Cuts at: 0 194 554 1050 1184 1224 1760 1841 2524
Size: 194 360 496 134 40 536 81 683
Cuts at: 2524 3050 3207 4098
Size: 526 157 891
Fragments arranged by size:
891 683 536 526 496 360 194 157
134 81 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3184 4098
Size: 3184 914
TatI w'GTAC_w
Cuts at: 0 248 599 956 1337 1805 2933 4098
Size: 248 351 357 381 468 1128 1165
Fragments arranged by size:
1165 1128 468 381 357 351 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2834 2866 4098
Size: 2834 32 1232
Fragments arranged by size:
2834 1232 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1680 4098
Size: 1680 2418
XhoI C'TCGA_G
Cuts at: 0 277 4098
Size: 277 3821
XmaI C'CCGG_G
Cuts at: 0 2691 4098
Size: 2691 1407
XmnI GAAnn'nnTTC
Cuts at: 0 331 3163 4098
Size: 331 2832 935
Fragments arranged by size:
2832 935 331
ZraI GAC'GTC
Cuts at: 0 1473 3579 4098
Size: 1473 2106 519
Fragments arranged by size:
2106 1473 519
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV FspI FspAI HaeII Hin4I HindIII KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PsrI
PstI PvuII SacII SanDI SapI ScaI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AlfI AloI ApaI AscI
AseI AsiSI BaeI BamHI BciVI BfrBI BlpI BmtI
BsaBI BsiWI BsmI BsmBI BspHI BspMI BstBI BstEII
BstZ17I CspCI DrdI EcoICRI EcoRI FalI FseI HincII
HpaI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PsiI PspOMI PspXI PvuI
RsrII SacI SalI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI