Restriction map for
pF1KB9116
(Linear) (Six-base) MAPSORT of: KIBB9116.seq Check: 8115 from: 1 to: 2316
KIBB9116.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AclI AA'CG_TT
Cuts at: 0 1192 2316
Size: 1192 1124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2120 2156 2316
Size: 2120 36 160
Fragments arranged by size:
2120 160 36
AleI CACnn'nnGTG
Cuts at: 0 1938 2316
Size: 1938 378
AlwNI CAG_nnn'CTG
Cuts at: 0 2202 2316
Size: 2202 114
ApoI r'AATT_y
Cuts at: 0 672 984 1472 2316
Size: 672 312 488 844
Fragments arranged by size:
844 672 488 312
AseI AT'TA_AT
Cuts at: 0 1681 2316
Size: 1681 635
AvaI C'yCGr_G
Cuts at: 0 157 2316
Size: 157 2159
AvrII C'CTAG_G
Cuts at: 0 235 2316
Size: 235 2081
BamHI G'GATC_C
Cuts at: 0 127 2316
Size: 127 2189
BanII G_rGCy'C
Cuts at: 0 1173 2316
Size: 1173 1143
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 2316
Size: 92 2224
BciVI GTATCCnnnnn_n'
Cuts at: 0 1143 1322 1599 2316
Size: 1143 179 277 717
Fragments arranged by size:
1143 717 277 179
BfrBI ATG'CAT
Cuts at: 0 1531 2316
Size: 1531 785
BglII A'GATC_T
Cuts at: 0 599 1970 2316
Size: 599 1371 346
Fragments arranged by size:
1371 599 346
BlpI GC'TnA_GC
Cuts at: 0 533 2316
Size: 533 1783
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 895 2316
Size: 618 277 1421
Fragments arranged by size:
1421 618 277
BmtI G_CTAG'C
Cuts at: 0 463 2316
Size: 463 1853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 2316
Size: 526 1790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1300 1515 2285 2316
Size: 83 1217 215 770 31
Fragments arranged by size:
1217 770 215 83 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 900 2316
Size: 153 747 1416
Fragments arranged by size:
1416 747 153
BsaAI yAC'GTr
Cuts at: 0 1461 2316
Size: 1461 855
BsaBI GATnn'nnATC
Cuts at: 0 604 2316
Size: 604 1712
BsaHI Gr'CG_yC
Cuts at: 0 2293 2316
Size: 2293 23
BsaWI w'CCGG_w
Cuts at: 0 1544 1935 2316
Size: 1544 391 381
Fragments arranged by size:
1544 391 381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 56 70 86 412 442 2316
Size: 40 16 14 16 326 30 1874
Fragments arranged by size:
1874 326 40 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 1424 2316
Size: 436 988 892
Fragments arranged by size:
988 892 436
BseYI C'CCAG_C
Cuts at: 0 271 1158 2316
Size: 271 887 1158
Fragments arranged by size:
1158 887 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 638 2125 2316
Size: 138 500 1487 191
Fragments arranged by size:
1487 500 191 138
BsmI GAATG_Cn'
Cuts at: 0 576 1731 2316
Size: 576 1155 585
Fragments arranged by size:
1155 585 576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 729 2316
Size: 729 1587
Bsp1286I G_dGCh'C
Cuts at: 0 1173 2316
Size: 1173 1143
BsrGI T'GTAC_A
Cuts at: 0 1621 2316
Size: 1621 695
BssHII G'CGCG_C
Cuts at: 0 281 2316
Size: 281 2035
BstAPI GCAn_nnn'nTGC
Cuts at: 0 762 2316
Size: 762 1554
BstBI TT'CG_AA
Cuts at: 0 1849 2316
Size: 1849 467
BstYI r'GATC_y
Cuts at: 0 127 559 599 1265 1970 2316
Size: 127 432 40 666 705 346
Fragments arranged by size:
705 666 432 346 127 40
Bsu36I CC'TnA_GG
Cuts at: 0 1164 2316
Size: 1164 1152
BtgI C'CryG_G
Cuts at: 0 1046 2316
Size: 1046 1270
BtsI GCAGTG_nn'
Cuts at: 0 405 2316
Size: 405 1911
DraI TTT'AAA
Cuts at: 0 1356 1733 2316
Size: 1356 377 583
Fragments arranged by size:
1356 583 377
DraIII CAC_nnn'GTG
Cuts at: 0 1909 2316
Size: 1909 407
EaeI y'GGCC_r
Cuts at: 0 2043 2316
Size: 2043 273
EarI CTCTTCn'nnn_
Cuts at: 0 992 1131 2316
Size: 992 139 1185
Fragments arranged by size:
1185 992 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1300 1515 2120 2156 2285 2316
Size: 83 1217 215 605 36 129 31
Fragments arranged by size:
1217 605 215 129 83 36 31
EcoO109I rG'GnC_Cy
Cuts at: 0 1056 2316
Size: 1056 1260
EcoRI G'AATT_C
Cuts at: 0 672 2316
Size: 672 1644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2054 2316
Size: 2054 262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 1597 1629 1687 1719 1885 1917
Size: 412 32 1153 32 58 32 166 32
Cuts at: 1917 1978 1998 2010 2030 2316
Size: 61 20 12 20 286
Fragments arranged by size:
1153 412 286 166 61 58 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 1190 2316
Size: 1190 1126
HpaI GTT'AAC
Cuts at: 0 1190 2316
Size: 1190 1126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 128 653 930 1390 1667 2316
Size: 105 23 525 277 460 277 649
Fragments arranged by size:
649 525 460 277 277 105 23
MscI TGG'CCA
Cuts at: 0 2045 2316
Size: 2045 271
MslI CAynn'nnrTG
Cuts at: 0 451 1526 1711 1938 2316
Size: 451 1075 185 227 378
Fragments arranged by size:
1075 451 378 227 185
MspA1I CmG'CkG
Cuts at: 0 489 1086 2202 2316
Size: 489 597 1116 114
Fragments arranged by size:
1116 597 489 114
NcoI C'CATG_G
Cuts at: 0 1046 2316
Size: 1046 1270
NdeI CA'TA_TG
Cuts at: 0 207 2316
Size: 207 2109
NheI G'CTAG_C
Cuts at: 0 459 2316
Size: 459 1857
NsiI A_TGCA'T
Cuts at: 0 1533 2316
Size: 1533 783
NspI r_CATG'y
Cuts at: 0 870 1531 1535 2316
Size: 870 661 4 781
Fragments arranged by size:
870 781 661 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 2202 2316
Size: 2202 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 365 397 2316
Size: 365 32 1919
Fragments arranged by size:
1919 365 32
PstI C_TGCA'G
Cuts at: 0 586 2316
Size: 586 1730
PvuII CAG'CTG
Cuts at: 0 489 2202 2316
Size: 489 1713 114
Fragments arranged by size:
1713 489 114
ScaI AGT'ACT
Cuts at: 0 1738 2316
Size: 1738 578
SexAI A'CCwGG_T
Cuts at: 0 1782 2316
Size: 1782 534
SfcI C'TryA_G
Cuts at: 0 582 2316
Size: 582 1734
SmlI C'TyrA_G
Cuts at: 0 132 879 2316
Size: 132 747 1437
Fragments arranged by size:
1437 747 132
SnaBI TAC'GTA
Cuts at: 0 1461 2316
Size: 1461 855
SphI G_CATG'C
Cuts at: 0 870 1531 2316
Size: 870 661 785
Fragments arranged by size:
870 785 661
SspI AAT'ATT
Cuts at: 0 1791 2316
Size: 1791 525
StyI C'CwwG_G
Cuts at: 0 219 235 1046 2316
Size: 219 16 811 1270
Fragments arranged by size:
1270 811 219 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1990 2316
Size: 1990 326
TatI w'GTAC_w
Cuts at: 0 1621 1736 2316
Size: 1621 115 580
Fragments arranged by size:
1621 580 115
Tth111I GACn'n_nGTC
Cuts at: 0 624 2316
Size: 624 1692
Enzymes that do cut:
AclI AcuI AleI AlwNI ApoI AseI AvaI AvrII
BamHI BanII BbsI BciVI BfrBI BglII BlpI BmrI
BmtI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BsrGI
BssHII BstAPI BstBI BstYI Bsu36I BtgI BtsI DraI
DraIII EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HpaI MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PflMI PpiI PstI PvuII ScaI
SexAI SfcI SmlI SnaBI SphI SspI StyI TaqII
TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI BaeI
BanI BbeI BbvCI BcgI BclI BglI Bme1580I BmgBI
Bpu10I BsaI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HindIII KasI KpnI MfeI
MluI NaeI NarI NgoMIV NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SpeI
SrfI StuI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI