Restriction map for
pF1KB9123
(Linear) (Six-base) MAPSORT of: KIBB9123.seq Check: 9035 from: 1 to: 1515
KIBB9123.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1389 1515
Size: 1389 126
AccI GT'mk_AC
Cuts at: 0 420 1366 1515
Size: 420 946 149
Fragments arranged by size:
946 420 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1049 1376 1436 1515
Size: 110 939 327 60 79
Fragments arranged by size:
939 327 110 79 60
AflIII A'CryG_T
Cuts at: 0 639 1515
Size: 639 876
AleI CACnn'nnGTG
Cuts at: 0 46 1515
Size: 46 1469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 161 1515
Size: 161 1354
ApoI r'AATT_y
Cuts at: 0 385 1515
Size: 385 1130
AseI AT'TA_AT
Cuts at: 0 1411 1515
Size: 1411 104
BamHI G'GATC_C
Cuts at: 0 651 684 1515
Size: 651 33 831
Fragments arranged by size:
831 651 33
BanI G'GyrC_C
Cuts at: 0 1113 1515
Size: 1113 402
BanII G_rGCy'C
Cuts at: 0 7 951 1515
Size: 7 944 564
Fragments arranged by size:
944 564 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 1515
Size: 116 1399
BbvCI CC'TCA_GC
Cuts at: 0 1056 1515
Size: 1056 459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1258 1292 1515
Size: 1258 34 223
Fragments arranged by size:
1258 223 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1342 1515
Size: 1342 173
BglII A'GATC_T
Cuts at: 0 220 1515
Size: 220 1295
BlpI GC'TnA_GC
Cuts at: 0 1250 1515
Size: 1250 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1025 1515
Size: 353 672 490
Fragments arranged by size:
672 490 353
Bpu10I CC'TnA_GC
Cuts at: 0 1056 1515
Size: 1056 459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 529 559 1515
Size: 67 30 432 30 956
Fragments arranged by size:
956 432 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 854 857 860 887 890 893 917 1022
Size: 854 3 3 27 3 3 24 105
Cuts at: 1022 1044 1133 1515
Size: 22 89 382
Fragments arranged by size:
854 382 105 89 27 24 22 3
3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 201 944 1367 1515
Size: 157 44 743 423 148
Fragments arranged by size:
743 423 157 148 44
BsiHKAI G_wGCw'C
Cuts at: 0 7 951 1251 1515
Size: 7 944 300 264
Fragments arranged by size:
944 300 264 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 951 1251 1515
Size: 7 944 300 264
Fragments arranged by size:
944 300 264 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1389 1515
Size: 1389 126
BstBI TT'CG_AA
Cuts at: 0 390 1515
Size: 390 1125
BstEII G'GTnAC_C
Cuts at: 0 39 1515
Size: 39 1476
BstXI CCAn_nnnn'nTGG
Cuts at: 0 634 1515
Size: 634 881
BstYI r'GATC_y
Cuts at: 0 220 447 651 684 1515
Size: 220 227 204 33 831
Fragments arranged by size:
831 227 220 204 33
BtgI C'CryG_G
Cuts at: 0 31 473 1515
Size: 31 442 1042
Fragments arranged by size:
1042 442 31
BtsI GCAGTG_nn'
Cuts at: 0 137 189 1515
Size: 137 52 1326
Fragments arranged by size:
1326 137 52
EaeI y'GGCC_r
Cuts at: 0 1279 1356 1515
Size: 1279 77 159
Fragments arranged by size:
1279 159 77
EarI CTCTTCn'nnn_
Cuts at: 0 94 580 912 1515
Size: 94 486 332 603
Fragments arranged by size:
603 486 332 94
EcoICRI GAG'CTC
Cuts at: 0 5 949 1515
Size: 5 944 566
Fragments arranged by size:
944 566 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 353 1025 1049 1376 1436 1515
Size: 110 243 672 24 327 60 79
Fragments arranged by size:
672 327 243 110 79 60 24
EcoO109I rG'GnC_Cy
Cuts at: 0 53 892 1069 1515
Size: 53 839 177 446
Fragments arranged by size:
839 446 177 53
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 370 1240 1515
Size: 370 870 275
Fragments arranged by size:
870 370 275
HaeII r_GCGC'y
Cuts at: 0 926 1515
Size: 926 589
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 1014 1046 1316 1348 1515
Size: 67 32 915 32 270 32 167
Fragments arranged by size:
915 270 167 67 32 32 32
HincII GTy'rAC
Cuts at: 0 421 537 1515
Size: 421 116 978
Fragments arranged by size:
978 421 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1359 1515
Size: 1222 137 156
Fragments arranged by size:
1222 156 137
MscI TGG'CCA
Cuts at: 0 1358 1515
Size: 1358 157
MslI CAynn'nnrTG
Cuts at: 0 46 1515
Size: 46 1469
MspA1I CmG'CkG
Cuts at: 0 33 958 1515
Size: 33 925 557
Fragments arranged by size:
925 557 33
NcoI C'CATG_G
Cuts at: 0 473 1515
Size: 473 1042
NspI r_CATG'y
Cuts at: 0 643 1515
Size: 643 872
PciI A'CATG_T
Cuts at: 0 639 1515
Size: 639 876
PpuMI rG'GwC_Cy
Cuts at: 0 53 892 1515
Size: 53 839 623
Fragments arranged by size:
839 623 53
PstI C_TGCA'G
Cuts at: 0 1209 1515
Size: 1209 306
PvuII CAG'CTG
Cuts at: 0 958 1515
Size: 958 557
SacI G_AGCT'C
Cuts at: 0 7 951 1515
Size: 7 944 564
Fragments arranged by size:
944 564 7
SacII CC_GC'GG
Cuts at: 0 34 1515
Size: 34 1481
SalI G'TCGA_C
Cuts at: 0 419 1515
Size: 419 1096
SanDI GG'GwC_CC
Cuts at: 0 53 1515
Size: 53 1462
SfcI C'TryA_G
Cuts at: 0 479 1205 1515
Size: 479 726 310
Fragments arranged by size:
726 479 310
SspI AAT'ATT
Cuts at: 0 239 1515
Size: 239 1276
StyI C'CwwG_G
Cuts at: 0 473 596 1515
Size: 473 123 919
Fragments arranged by size:
919 473 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1393 1401 1515
Size: 1393 8 114
Fragments arranged by size:
1393 114 8
Tth111I GACn'n_nGTC
Cuts at: 0 120 1515
Size: 120 1395
XmnI GAAnn'nnTTC
Cuts at: 0 703 1515
Size: 703 812
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI ApoI AseI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BglII
BlpI BpmI Bpu10I BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspMI BstBI BstEII BstXI BstYI BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI NspI PciI PpuMI
PstI PvuII SacI SacII SalI SanDI SfcI SspI
StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BbeI BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI XbaI XcmI XhoI XmaI ZraI