Restriction map for
pF1KB9127
(Linear) (Six-base) MAPSORT of: KIBB9127.seq Check: 4501 from: 1 to: 2457
KIBB9127.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AclI AA'CG_TT
Cuts at: 0 1864 2200 2457
Size: 1864 336 257
Fragments arranged by size:
1864 336 257
AleI CACnn'nnGTG
Cuts at: 0 902 962 1070 1802 2222 2457
Size: 902 60 108 732 420 235
Fragments arranged by size:
902 732 420 235 108 60
AlwNI CAG_nnn'CTG
Cuts at: 0 1097 2457
Size: 1097 1360
ApaLI G'TGCA_C
Cuts at: 0 1805 2057 2225 2457
Size: 1805 252 168 232
Fragments arranged by size:
1805 252 232 168
ApoI r'AATT_y
Cuts at: 0 6 264 319 386 415 633 659 809
Size: 6 258 55 67 29 218 26 150
Cuts at: 809 893 1061 1145 1229 1313 1397 1565 1649
Size: 84 168 84 84 84 84 168 84
Cuts at: 1649 1733 1817 1901 2153 2237 2295 2321 2457
Size: 84 84 84 252 84 58 26 136
Fragments arranged by size:
258 252 218 168 168 150 136 84
84 84 84 84 84 84 84 84
84 67 58 55 29 26 26 6
AseI AT'TA_AT
Cuts at: 0 179 2457
Size: 179 2278
BanII G_rGCy'C
Cuts at: 0 280 2457
Size: 280 2177
BbsI GAAGACnn'nnnn_
Cuts at: 0 787 1546 1951 2457
Size: 787 759 405 506
Fragments arranged by size:
787 759 506 405
BfrBI ATG'CAT
Cuts at: 0 2412 2457
Size: 2412 45
BglI GCCn_nnn'nGGC
Cuts at: 0 1888 2457
Size: 1888 569
BglII A'GATC_T
Cuts at: 0 703 1273 2457
Size: 703 570 1184
Fragments arranged by size:
1184 703 570
BlpI GC'TnA_GC
Cuts at: 0 279 2457
Size: 279 2178
Bme1580I G_kGCm'C
Cuts at: 0 1809 1893 2061 2229 2457
Size: 1809 84 168 168 228
Fragments arranged by size:
1809 228 168 168 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 1835 2457
Size: 1835 622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 2457
Size: 332 2125
Bpu10I CC'TnA_GC
Cuts at: 0 1992 2457
Size: 1992 465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1198 2457
Size: 1198 1259
BsiHKAI G_wGCw'C
Cuts at: 0 280 885 1053 1137 1305 1473 1809 1977
Size: 280 605 168 84 168 168 336 168
Cuts at: 1977 2061 2229 2457
Size: 84 168 228
Fragments arranged by size:
605 336 280 228 168 168 168 168
168 84 84
BsmI GAATG_Cn'
Cuts at: 0 1093 2017 2278 2414 2457
Size: 1093 924 261 136 43
Fragments arranged by size:
1093 924 261 136 43
Bsp1286I G_dGCh'C
Cuts at: 0 280 885 1053 1137 1305 1473 1809 1893
Size: 280 605 168 84 168 168 336 84
Cuts at: 1893 1977 2061 2229 2457
Size: 84 84 168 228
Fragments arranged by size:
605 336 280 228 168 168 168 168
84 84 84 84
BsrGI T'GTAC_A
Cuts at: 0 340 966 2457
Size: 340 626 1491
Fragments arranged by size:
1491 626 340
BstYI r'GATC_y
Cuts at: 0 703 1273 2457
Size: 703 570 1184
Fragments arranged by size:
1184 703 570
Bsu36I CC'TnA_GG
Cuts at: 0 1885 2457
Size: 1885 572
BtsI GCAGTG_nn'
Cuts at: 0 774 1098 2022 2457
Size: 774 324 924 435
Fragments arranged by size:
924 774 435 324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 102 137 678 713 2457
Size: 102 35 541 35 1744
Fragments arranged by size:
1744 541 102 35 35
EcoICRI GAG'CTC
Cuts at: 0 278 2457
Size: 278 2179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 2457
Size: 332 2125
EcoO109I rG'GnC_Cy
Cuts at: 0 491 2457
Size: 491 1966
EcoRI G'AATT_C
Cuts at: 0 386 633 659 809 893 1061 1145 1229
Size: 386 247 26 150 84 168 84 84
Cuts at: 1229 1313 1397 1565 1649 1733 1817 1901 2153
Size: 84 84 168 84 84 84 84 252
Cuts at: 2153 2237 2321 2457
Size: 84 84 136
Fragments arranged by size:
386 252 247 168 168 150 136 84
84 84 84 84 84 84 84 84
84 84 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 774 806 858 890 1038 1070 1290 1322
Size: 774 32 52 32 148 32 220 32
Cuts at: 1322 1950 1982 2457
Size: 628 32 475
Fragments arranged by size:
774 628 475 220 148 52 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 620 2282 2457
Size: 620 1662 175
Fragments arranged by size:
1662 620 175
MslI CAynn'nnrTG
Cuts at: 0 145 902 962 1070 1802 2222 2407 2457
Size: 145 757 60 108 732 420 185 50
Fragments arranged by size:
757 732 420 185 145 108 60 50
MspA1I CmG'CkG
Cuts at: 0 118 2457
Size: 118 2339
NdeI CA'TA_TG
Cuts at: 0 2049 2457
Size: 2049 408
NsiI A_TGCA'T
Cuts at: 0 2414 2457
Size: 2414 43
NspI r_CATG'y
Cuts at: 0 2438 2457
Size: 2438 19
PfoI T'CCnGG_A
Cuts at: 0 570 2457
Size: 570 1887
SacI G_AGCT'C
Cuts at: 0 280 2457
Size: 280 2177
SmlI C'TyrA_G
Cuts at: 0 1213 2457
Size: 1213 1244
TatI w'GTAC_w
Cuts at: 0 291 340 966 1552 2142 2310 2457
Size: 291 49 626 586 590 168 147
Fragments arranged by size:
626 590 586 291 168 147 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 87 119 566 598 2457
Size: 87 32 447 32 1859
Fragments arranged by size:
1859 447 87 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 622 856 940 1612 1780 1948 2032 2200
Size: 622 234 84 672 168 168 84 168
Cuts at: 2200 2457
Size: 257
Fragments arranged by size:
672 622 257 234 168 168 168 84
84
Enzymes that do cut:
AclI AleI AlwNI ApaLI ApoI AseI BanII BbsI
BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsiHKAI BsmI Bsp1286I BsrGI BstYI Bsu36I BtsI
CspCI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII MslI
MspA1I NdeI NsiI NspI PfoI SacI SmlI TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI