Restriction map for
pF1KB9131
(Linear) (Six-base) MAPSORT of: KIBB9131.seq Check: 4286 from: 1 to: 1737
KIBB9131.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1737
Size: 442 1295
AhdI GACnn_n'nnGTC
Cuts at: 0 263 1737
Size: 263 1474
ApaLI G'TGCA_C
Cuts at: 0 245 1601 1737
Size: 245 1356 136
Fragments arranged by size:
1356 245 136
ApoI r'AATT_y
Cuts at: 0 283 1210 1252 1462 1737
Size: 283 927 42 210 275
Fragments arranged by size:
927 283 275 210 42
BamHI G'GATC_C
Cuts at: 0 1176 1737
Size: 1176 561
BanII G_rGCy'C
Cuts at: 0 780 1715 1737
Size: 780 935 22
Fragments arranged by size:
935 780 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 592 1737
Size: 592 1145
BciVI GTATCCnnnnn_n'
Cuts at: 0 780 1737
Size: 780 957
BclI T'GATC_A
Cuts at: 0 894 1737
Size: 894 843
BfrBI ATG'CAT
Cuts at: 0 984 1140 1737
Size: 984 156 597
Fragments arranged by size:
984 597 156
BglII A'GATC_T
Cuts at: 0 1281 1677 1737
Size: 1281 396 60
Fragments arranged by size:
1281 396 60
BlpI GC'TnA_GC
Cuts at: 0 48 913 1737
Size: 48 865 824
Fragments arranged by size:
865 824 48
Bme1580I G_kGCm'C
Cuts at: 0 249 1605 1737
Size: 249 1356 132
Fragments arranged by size:
1356 249 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1181 1244 1737
Size: 1181 63 493
Fragments arranged by size:
1181 493 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1148 1214 1737
Size: 718 430 66 523
Fragments arranged by size:
718 523 430 66
Bpu10I CC'TnA_GC
Cuts at: 0 539 1737
Size: 539 1198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1737
Size: 39 1698
BsaHI Gr'CG_yC
Cuts at: 0 328 1737
Size: 328 1409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 99 716 863 1723 1737
Size: 96 3 617 147 860 14
Fragments arranged by size:
860 617 147 96 14 3
BseYI C'CCAG_C
Cuts at: 0 1318 1737
Size: 1318 419
BsiHKAI G_wGCw'C
Cuts at: 0 249 780 1605 1715 1737
Size: 249 531 825 110 22
Fragments arranged by size:
825 531 249 110 22
BsmI GAATG_Cn'
Cuts at: 0 15 1142 1737
Size: 15 1127 595
Fragments arranged by size:
1127 595 15
Bsp1286I G_dGCh'C
Cuts at: 0 249 780 1605 1715 1737
Size: 249 531 825 110 22
Fragments arranged by size:
825 531 249 110 22
BspHI T'CATG_A
Cuts at: 0 645 891 1737
Size: 645 246 846
Fragments arranged by size:
846 645 246
BsrDI GCAATG_nn'
Cuts at: 0 478 587 897 912 1737
Size: 478 109 310 15 825
Fragments arranged by size:
825 478 310 109 15
BstEII G'GTnAC_C
Cuts at: 0 768 1737
Size: 768 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 610 870 1737
Size: 610 260 867
Fragments arranged by size:
867 610 260
BstYI r'GATC_y
Cuts at: 0 1176 1281 1440 1677 1737
Size: 1176 105 159 237 60
Fragments arranged by size:
1176 237 159 105 60
BtsI GCAGTG_nn'
Cuts at: 0 1241 1737
Size: 1241 496
ClaI AT'CG_AT
Cuts at: 0 1545 1737
Size: 1545 192
DraI TTT'AAA
Cuts at: 0 887 1313 1691 1725 1737
Size: 887 426 378 34 12
Fragments arranged by size:
887 426 378 34 12
EarI CTCTTCn'nnn_
Cuts at: 0 117 726 1496 1737
Size: 117 609 770 241
Fragments arranged by size:
770 609 241 117
EcoICRI GAG'CTC
Cuts at: 0 778 1713 1737
Size: 778 935 24
Fragments arranged by size:
935 778 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 718 1148 1214 1737
Size: 442 276 430 66 523
Fragments arranged by size:
523 442 430 276 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1312 1737
Size: 1312 425
EcoO109I rG'GnC_Cy
Cuts at: 0 1610 1737
Size: 1610 127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 393 807 1737
Size: 393 414 930
Fragments arranged by size:
930 414 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 313 345 762 794 1184 1216
Size: 249 32 32 32 417 32 390 32
Cuts at: 1216 1408 1440 1669 1701 1737
Size: 192 32 229 32 36
Fragments arranged by size:
417 390 249 229 192 36 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1305 1566 1737
Size: 1305 261 171
Fragments arranged by size:
1305 261 171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1512 1737
Size: 480 1032 225
Fragments arranged by size:
1032 480 225
MslI CAynn'nnrTG
Cuts at: 0 272 953 1634 1737
Size: 272 681 681 103
Fragments arranged by size:
681 681 272 103
MspA1I CmG'CkG
Cuts at: 0 678 826 1522 1737
Size: 678 148 696 215
Fragments arranged by size:
696 678 215 148
NdeI CA'TA_TG
Cuts at: 0 1170 1737
Size: 1170 567
NsiI A_TGCA'T
Cuts at: 0 986 1142 1737
Size: 986 156 595
Fragments arranged by size:
986 595 156
NspI r_CATG'y
Cuts at: 0 837 1701 1737
Size: 837 864 36
Fragments arranged by size:
864 837 36
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 503 535 1737
Size: 503 32 1202
Fragments arranged by size:
1202 503 32
PpuMI rG'GwC_Cy
Cuts at: 0 1610 1737
Size: 1610 127
PstI C_TGCA'G
Cuts at: 0 1542 1737
Size: 1542 195
PvuII CAG'CTG
Cuts at: 0 678 826 1522 1737
Size: 678 148 696 215
Fragments arranged by size:
696 678 215 148
SacI G_AGCT'C
Cuts at: 0 780 1715 1737
Size: 780 935 22
Fragments arranged by size:
935 780 22
SapI GCTCTTCn'nnn_
Cuts at: 0 726 1737
Size: 726 1011
SexAI A'CCwGG_T
Cuts at: 0 1298 1737
Size: 1298 439
SfcI C'TryA_G
Cuts at: 0 930 1538 1737
Size: 930 608 199
Fragments arranged by size:
930 608 199
SmlI C'TyrA_G
Cuts at: 0 54 1737
Size: 54 1683
SphI G_CATG'C
Cuts at: 0 837 1737
Size: 837 900
SspI AAT'ATT
Cuts at: 0 186 1737
Size: 186 1551
StuI AGG'CCT
Cuts at: 0 1642 1737
Size: 1642 95
SwaI ATTT'AAAT
Cuts at: 0 887 1737
Size: 887 850
TatI w'GTAC_w
Cuts at: 0 407 1737
Size: 407 1330
Tth111I GACn'n_nGTC
Cuts at: 0 1300 1737
Size: 1300 437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 1737
Size: 693 1044
XmnI GAAnn'nnTTC
Cuts at: 0 94 857 1737
Size: 94 763 880
Fragments arranged by size:
880 763 94
Enzymes that do cut:
AcuI AhdI ApaLI ApoI BamHI BanII BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BstEII BstXI BstYI BtsI ClaI DraI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MmeI
MslI MspA1I NdeI NsiI NspI PpiI PpuMI PstI
PvuII SacI SapI SexAI SfcI SmlI SphI SspI
StuI SwaI TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BglI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgI BtgZI CspCI DraIII DrdI EaeI EagI EciI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StyI
TaqII TstI XbaI XhoI XmaI ZraI