Restriction map for
pF1KB9166
(Linear) (Six-base) MAPSORT of: KIBB9166.seq Check: 8123 from: 1 to: 6009
KIBB9166.orf
With 182 enzymes: SgfI *
October 29, 2012 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 127 751 904 1000 1114 1291 2005 2155
Size: 127 624 153 96 114 177 714 150
Cuts at: 2155 2269 2839 3256 6009
Size: 114 570 417 2753
Fragments arranged by size:
2753 714 624 570 417 177 153 150
127 114 114 96
AatII G_ACGT'C
Cuts at: 0 2690 5417 6009
Size: 2690 2727 592
Fragments arranged by size:
2727 2690 592
AccI GT'mk_AC
Cuts at: 0 2723 4419 5651 6009
Size: 2723 1696 1232 358
Fragments arranged by size:
2723 1696 1232 358
Acc65I G'GTAC_C
Cuts at: 0 2338 6009
Size: 2338 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3725 3878 4061 4561 4640 6009
Size: 3725 153 183 500 79 1369
Fragments arranged by size:
3725 1369 500 183 153 79
AflIII A'CryG_T
Cuts at: 0 1181 6009
Size: 1181 4828
AhdI GACnn_n'nnGTC
Cuts at: 0 2195 6009
Size: 2195 3814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 1633 3953 4937 5237 6009
Size: 726 907 2320 984 300 772
Fragments arranged by size:
2320 984 907 772 726 300
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2934 2966 6009
Size: 2934 32 3043
Fragments arranged by size:
3043 2934 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1296 2441 2529 3058 3102 3933 4107 4591
Size: 1296 1145 88 529 44 831 174 484
Cuts at: 4591 5055 5355 6009
Size: 464 300 654
Fragments arranged by size:
1296 1145 831 654 529 484 464 300
174 88 44
ApaI G_GGCC'C
Cuts at: 0 1280 2285 2398 2591 3264 3463 3503 3884
Size: 1280 1005 113 193 673 199 40 381
Cuts at: 3884 4035 5516 6009
Size: 151 1481 493
Fragments arranged by size:
1481 1280 1005 673 493 381 199 193
151 113 40
ApaLI G'TGCA_C
Cuts at: 0 306 6009
Size: 306 5703
AvaI C'yCGr_G
Cuts at: 0 411 1095 3579 3947 4034 4061 5036 5240
Size: 411 684 2484 368 87 27 975 204
Cuts at: 5240 5375 5700 6009
Size: 135 325 309
Fragments arranged by size:
2484 975 684 411 368 325 309 204
135 87 27
AvrII C'CTAG_G
Cuts at: 0 261 718 4383 6009
Size: 261 457 3665 1626
Fragments arranged by size:
3665 1626 457 261
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2330 2363 6009
Size: 2330 33 3646
Fragments arranged by size:
3646 2330 33
BamHI G'GATC_C
Cuts at: 0 1099 6009
Size: 1099 4910
BanI G'GyrC_C
Cuts at: 0 115 622 739 988 1633 2338 2359 6009
Size: 115 507 117 249 645 705 21 3650
Fragments arranged by size:
3650 705 645 507 249 117 115 21
BanII G_rGCy'C
Cuts at: 0 269 649 1126 1280 2285 2398 2591 3264
Size: 269 380 477 154 1005 113 193 673
Cuts at: 3264 3463 3503 3884 3913 4035 4260 4268 4392
Size: 199 40 381 29 122 225 8 124
Cuts at: 4392 4440 4844 5516 5571 6009
Size: 48 404 672 55 438
Fragments arranged by size:
1005 673 672 477 438 404 381 380
269 225 199 193 154 124 122 113
55 48 40 29 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 362 1184 5147 6009
Size: 362 822 3963 862
Fragments arranged by size:
3963 862 822 362
BbvCI CC'TCA_GC
Cuts at: 0 1554 6009
Size: 1554 4455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 547 581 3461 3495 5263 5297 6009
Size: 547 34 2880 34 1768 34 712
Fragments arranged by size:
2880 1768 712 547 34 34 34
BclI T'GATC_A
Cuts at: 0 1422 3780 6009
Size: 1422 2358 2229
Fragments arranged by size:
2358 2229 1422
BglI GCCn_nnn'nGGC
Cuts at: 0 320 551 620 1508 2156 2246 2501 2987
Size: 320 231 69 888 648 90 255 486
Cuts at: 2987 3353 4387 5131 6009
Size: 366 1034 744 878
Fragments arranged by size:
1034 888 878 744 648 486 366 320
255 231 90 69
Bme1580I G_kGCm'C
Cuts at: 0 162 310 742 868 991 1095 1280 1977
Size: 162 148 432 126 123 104 185 697
Cuts at: 1977 2012 2103 2285 2398 2547 2591 2871 3264
Size: 35 91 182 113 149 44 280 393
Cuts at: 3264 3463 3503 3800 3884 4035 4897 5516 5552
Size: 199 40 297 84 151 862 619 36
Cuts at: 5552 6009
Size: 457
Fragments arranged by size:
862 697 619 457 432 393 297 280
199 185 182 162 151 149 148 126
123 113 104 91 84 44 40 36
35
BmgBI CAC'GTC
Cuts at: 0 182 2040 3018 5178 6009
Size: 182 1858 978 2160 831
Fragments arranged by size:
2160 1858 978 831 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 2280 2594 2840 3600 4351 4907 5226 5445
Size: 2280 314 246 760 751 556 319 219
Cuts at: 5445 6009
Size: 564
Fragments arranged by size:
2280 760 751 564 556 319 314 246
219
BmtI G_CTAG'C
Cuts at: 0 5404 6009
Size: 5404 605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 149 783 1455 1569 4140 6009
Size: 149 634 672 114 2571 1869
Fragments arranged by size:
2571 1869 672 634 149 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1730 2264 2563 2596 2969 2981 3479 3728
Size: 1730 534 299 33 373 12 498 249
Cuts at: 3728 3740 4282 4387 4988 5245 6009
Size: 12 542 105 601 257 764
Fragments arranged by size:
1730 764 601 542 534 498 373 299
257 249 105 33 12 12
Bpu10I CC'TnA_GC
Cuts at: 0 124 1554 3317 4584 4838 6009
Size: 124 1430 1763 1267 254 1171
Fragments arranged by size:
1763 1430 1267 1171 254 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4516 4981 6009
Size: 4516 465 1028
Fragments arranged by size:
4516 1028 465
BsaI GGTCTCn'nnnn_
Cuts at: 0 1531 3436 3730 4111 4534 4661 4897 5835
Size: 1531 1905 294 381 423 127 236 938
Cuts at: 5835 6009
Size: 174
Fragments arranged by size:
1905 1531 938 423 381 294 236 174
127
BsaAI yAC'GTr
Cuts at: 0 2790 4643 6009
Size: 2790 1853 1366
Fragments arranged by size:
2790 1853 1366
BsaHI Gr'CG_yC
Cuts at: 0 2687 4416 4422 4497 5305 5414 6009
Size: 2687 1729 6 75 808 109 595
Fragments arranged by size:
2687 1729 808 595 109 75 6
BsaWI w'CCGG_w
Cuts at: 0 2509 6009
Size: 2509 3500
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1390 1420 2250 2280 2934 2964 4592 4622
Size: 1390 30 830 30 654 30 1628 30
Cuts at: 4622 5813 5843 6009
Size: 1191 30 166
Fragments arranged by size:
1628 1390 1191 830 654 166 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1072 1660 2197 4609 4618 4694 5732 6008
Size: 1072 588 537 2412 9 76 1038 276
Cuts at: 6008 6009
Size: 1
Fragments arranged by size:
2412 1072 1038 588 537 276 76 9
1
BseYI C'CCAG_C
Cuts at: 0 212 271 295 347 923 1037 1316 1442
Size: 212 59 24 52 576 114 279 126
Cuts at: 1442 2123 2422 2840 3833 3915 4135 5348 5358
Size: 681 299 418 993 82 220 1213 10
Cuts at: 5358 5364 5395 5464 5509 5961 6009
Size: 6 31 69 45 452 48
Fragments arranged by size:
1213 993 681 576 452 418 299 279
220 212 126 114 82 69 59 52
48 45 31 24 10 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1683 1977 2075 2127 2241 2450 2651 3735
Size: 1683 294 98 52 114 209 201 1084
Cuts at: 3735 4897 5197 6009
Size: 1162 300 812
Fragments arranged by size:
1683 1162 1084 812 300 294 209 201
114 98 52
BsiEI CG_ry'CG
Cuts at: 0 5739 6009
Size: 5739 270
BsiHKAI G_wGCw'C
Cuts at: 0 310 430 1890 4354 4440 4585 6009
Size: 310 120 1460 2464 86 145 1424
Fragments arranged by size:
2464 1460 1424 310 145 120 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 2694 3008 6009
Size: 172 2522 314 3001
Fragments arranged by size:
3001 2522 314 172
Bsp1286I G_dGCh'C
Cuts at: 0 162 269 310 430 649 742 868 991
Size: 162 107 41 120 219 93 126 123
Cuts at: 991 1095 1126 1280 1890 1977 2012 2103 2285
Size: 104 31 154 610 87 35 91 182
Cuts at: 2285 2398 2547 2591 2871 3264 3463 3503 3800
Size: 113 149 44 280 393 199 40 297
Cuts at: 3800 3884 3913 4035 4260 4268 4354 4392 4440
Size: 84 29 122 225 8 86 38 48
Cuts at: 4440 4585 4844 4897 5516 5552 5571 6009
Size: 145 259 53 619 36 19 438
Fragments arranged by size:
619 610 438 393 297 280 259 225
219 199 182 162 154 149 145 126
123 122 120 113 107 104 93 91
87 86 84 53 48 44 41 40
38 36 35 31 29 19 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 157 751 904 1000 1114 1291 1598
Size: 127 30 594 153 96 114 177 307
Cuts at: 1598 2005 2122 2155 2269 2467 2839 3256 6009
Size: 407 117 33 114 198 372 417 2753
Fragments arranged by size:
2753 594 417 407 372 307 198 177
153 127 117 114 114 96 33 30
BsrBI CCG'CTC
Cuts at: 0 256 5252 5335 5931 6009
Size: 256 4996 83 596 78
Fragments arranged by size:
4996 596 256 83 78
BsrDI GCAATG_nn'
Cuts at: 0 5685 6009
Size: 5685 324
BsrFI r'CCGG_y
Cuts at: 0 4499 5406 6009
Size: 4499 907 603
Fragments arranged by size:
4499 907 603
BssHII G'CGCG_C
Cuts at: 0 5523 6009
Size: 5523 486
BssSI C'ACGA_G
Cuts at: 0 5609 6009
Size: 5609 400
BstAPI GCAn_nnn'nTGC
Cuts at: 0 629 2098 2777 4321 6009
Size: 629 1469 679 1544 1688
Fragments arranged by size:
1688 1544 1469 679 629
BstBI TT'CG_AA
Cuts at: 0 730 6009
Size: 730 5279
BstXI CCAn_nnnn'nTGG
Cuts at: 0 914 2131 2835 2848 3453 3629 6009
Size: 914 1217 704 13 605 176 2380
Fragments arranged by size:
2380 1217 914 704 605 176 13
BstYI r'GATC_y
Cuts at: 0 530 1099 2020 5439 6009
Size: 530 569 921 3419 570
Fragments arranged by size:
3419 921 570 569 530
Bsu36I CC'TnA_GG
Cuts at: 0 1982 2474 4592 4685 6009
Size: 1982 492 2118 93 1324
Fragments arranged by size:
2118 1982 1324 492 93
BtgI C'CryG_G
Cuts at: 0 1203 2004 2103 2381 2937 3291 3446 4746
Size: 1203 801 99 278 556 354 155 1300
Cuts at: 4746 4889 6009
Size: 143 1120
Fragments arranged by size:
1300 1203 1120 801 556 354 278 155
143 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3575 4255 6009
Size: 3575 680 1754
Fragments arranged by size:
3575 1754 680
BtsI GCAGTG_nn'
Cuts at: 0 13 463 540 972 1017 1491 1699 1719
Size: 13 450 77 432 45 474 208 20
Cuts at: 1719 2010 2571 2598 2748 3056 3219 3282 3579
Size: 291 561 27 150 308 163 63 297
Cuts at: 3579 3688 3818 4256 4282 5084 6009
Size: 109 130 438 26 802 925
Fragments arranged by size:
925 802 561 474 450 438 432 308
297 291 208 163 150 130 109 77
63 45 27 26 20 13
ClaI AT'CG_AT
Cuts at: 0 1655 6009
Size: 1655 4354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1092 1127 1561 1596 6009
Size: 1092 35 434 35 4413
Fragments arranged by size:
4413 1092 434 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 691 1023 2098 3340 3697 4890 6009
Size: 691 332 1075 1242 357 1193 1119
Fragments arranged by size:
1242 1193 1119 1075 691 357 332
DrdI GACnn_nn'nnGTC
Cuts at: 0 838 6009
Size: 838 5171
EaeI y'GGCC_r
Cuts at: 0 515 3077 4361 4936 5736 6009
Size: 515 2562 1284 575 800 273
Fragments arranged by size:
2562 1284 800 575 515 273
EagI C'GGCC_G
Cuts at: 0 5736 6009
Size: 5736 273
EarI CTCTTCn'nnn_
Cuts at: 0 2697 3673 3701 4397 6009
Size: 2697 976 28 696 1612
Fragments arranged by size:
2697 1612 976 696 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2407 5425 5633 6009
Size: 2407 3018 208 376
Fragments arranged by size:
3018 2407 376 208
EcoICRI GAG'CTC
Cuts at: 0 4438 6009
Size: 4438 1571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1730 2264 2563 2596 2969 2981 3479 3725
Size: 1730 534 299 33 373 12 498 246
Cuts at: 3725 3728 3740 3878 4061 4282 4387 4561 4640
Size: 3 12 138 183 221 105 174 79
Cuts at: 4640 4988 5245 6009
Size: 348 257 764
Fragments arranged by size:
1730 764 534 498 373 348 299 257
246 221 183 174 138 105 79 33
12 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2779 3320 4111 4304 4960 6009
Size: 2779 541 791 193 656 1049
Fragments arranged by size:
2779 1049 791 656 541 193
EcoO109I rG'GnC_Cy
Cuts at: 0 146 600 607 706 964 1276 1293 2111
Size: 146 454 7 99 258 312 17 818
Cuts at: 2111 2167 2281 2282 2394 2479 2588 3176 3241
Size: 56 114 1 112 85 109 588 65
Cuts at: 3241 3260 3261 3364 3459 3499 3500 3554 3867
Size: 19 1 103 95 40 1 54 313
Cuts at: 3867 3880 3881 4031 4065 4209 4588 4808 4868
Size: 13 1 150 34 144 379 220 60
Cuts at: 4868 5513 5605 5675 6009
Size: 645 92 70 334
Fragments arranged by size:
818 645 588 454 379 334 313 312
258 220 150 146 144 114 112 109
103 99 95 92 85 70 65 60
56 54 40 34 19 17 13 7
1 1 1 1
EcoRV GAT'ATC
Cuts at: 0 1653 6009
Size: 1653 4356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 265 3342 6009
Size: 265 3077 2667
Fragments arranged by size:
3077 2667 265
HaeII r_GCGC'y
Cuts at: 0 4681 5407 6009
Size: 4681 726 602
Fragments arranged by size:
4681 726 602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2181 2213 2613 2645 5813 5845 5889 5920
Size: 2181 32 400 32 3168 32 44 31
Cuts at: 5920 5921 5952 6009
Size: 1 31 57
Fragments arranged by size:
3168 2181 400 57 44 32 32 32
31 31 1
HincII GTy'rAC
Cuts at: 0 585 2724 4420 6009
Size: 585 2139 1696 1589
Fragments arranged by size:
2139 1696 1589 585
KpnI G_GTAC'C
Cuts at: 0 2342 6009
Size: 2342 3667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1779 2026 5912 6009
Size: 1779 247 3886 97
Fragments arranged by size:
3886 1779 247 97
MscI TGG'CCA
Cuts at: 0 517 3079 6009
Size: 517 2562 2930
Fragments arranged by size:
2930 2562 517
MslI CAynn'nnrTG
Cuts at: 0 569 1208 2031 2833 2936 4301 6009
Size: 569 639 823 802 103 1365 1708
Fragments arranged by size:
1708 1365 823 802 639 569 103
MspA1I CmG'CkG
Cuts at: 0 236 395 750 1808 2426 2486 2519 2729
Size: 236 159 355 1058 618 60 33 210
Cuts at: 2729 3434 3933 4172 4199 4945 6009
Size: 705 499 239 27 746 1064
Fragments arranged by size:
1064 1058 746 705 618 499 355 239
236 210 159 60 33 27
NaeI GCC'GGC
Cuts at: 0 4501 5408 6009
Size: 4501 907 601
Fragments arranged by size:
4501 907 601
NcoI C'CATG_G
Cuts at: 0 1203 2103 2381 2937 6009
Size: 1203 900 278 556 3072
Fragments arranged by size:
3072 1203 900 556 278
NgoMIV G'CCGG_C
Cuts at: 0 4499 5406 6009
Size: 4499 907 603
Fragments arranged by size:
4499 907 603
NheI G'CTAG_C
Cuts at: 0 5400 6009
Size: 5400 609
NspI r_CATG'y
Cuts at: 0 438 1185 5760 6009
Size: 438 747 4575 249
Fragments arranged by size:
4575 747 438 249
PasI CC'CwG_GG
Cuts at: 0 642 960 3204 4107 4205 4213 4844 5865
Size: 642 318 2244 903 98 8 631 1021
Cuts at: 5865 6009
Size: 144
Fragments arranged by size:
2244 1021 903 642 631 318 144 98
8
PciI A'CATG_T
Cuts at: 0 1181 6009
Size: 1181 4828
PflMI CCAn_nnn'nTGG
Cuts at: 0 1296 3075 6009
Size: 1296 1779 2934
Fragments arranged by size:
2934 1779 1296
PfoI T'CCnGG_A
Cuts at: 0 596 1821 3671 4804 6009
Size: 596 1225 1850 1133 1205
Fragments arranged by size:
1850 1225 1205 1133 596
PmlI CAC'GTG
Cuts at: 0 2790 4643 6009
Size: 2790 1853 1366
Fragments arranged by size:
2790 1853 1366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2934 2966 6009
Size: 2934 32 3043
Fragments arranged by size:
3043 2934 32
PpuMI rG'GwC_Cy
Cuts at: 0 146 600 607 706 964 1293 2111 2167
Size: 146 454 7 99 258 329 818 56
Cuts at: 2167 2479 3176 3364 3867 4065 4209 4808 6009
Size: 312 697 188 503 198 144 599 1201
Fragments arranged by size:
1201 818 697 599 503 454 329 312
258 198 188 146 144 99 56 7
PshAI GACnn'nnGTC
Cuts at: 0 362 1184 1304 1790 3026 5954 6009
Size: 362 822 120 486 1236 2928 55
Fragments arranged by size:
2928 1236 822 486 362 120 55
PspOMI G'GGCC_C
Cuts at: 0 1276 2281 2394 2587 3260 3459 3499 3880
Size: 1276 1005 113 193 673 199 40 381
Cuts at: 3880 4031 5512 6009
Size: 151 1481 497
Fragments arranged by size:
1481 1276 1005 673 497 381 199 193
151 113 40
PstI C_TGCA'G
Cuts at: 0 979 3063 3253 3574 3825 3853 4251 5079
Size: 979 2084 190 321 251 28 398 828
Cuts at: 5079 5633 5754 6009
Size: 554 121 255
Fragments arranged by size:
2084 979 828 554 398 321 255 251
190 121 28
PvuII CAG'CTG
Cuts at: 0 236 750 1808 2426 2519 3434 3933 6009
Size: 236 514 1058 618 93 915 499 2076
Fragments arranged by size:
2076 1058 915 618 514 499 236 93
SacI G_AGCT'C
Cuts at: 0 4440 6009
Size: 4440 1569
SalI G'TCGA_C
Cuts at: 0 2722 4418 6009
Size: 2722 1696 1591
Fragments arranged by size:
2722 1696 1591
SanDI GG'GwC_CC
Cuts at: 0 964 2479 3867 4065 4209 6009
Size: 964 1515 1388 198 144 1800
Fragments arranged by size:
1800 1515 1388 964 198 144
SapI GCTCTTCn'nnn_
Cuts at: 0 4397 6009
Size: 4397 1612
ScaI AGT'ACT
Cuts at: 0 3772 6009
Size: 3772 2237
SexAI A'CCwGG_T
Cuts at: 0 5144 6009
Size: 5144 865
SfcI C'TryA_G
Cuts at: 0 975 2153 2175 2442 3059 3249 3570 3821
Size: 975 1178 22 267 617 190 321 251
Cuts at: 3821 3849 4247 5075 5629 5750 6009
Size: 28 398 828 554 121 259
Fragments arranged by size:
1178 975 828 617 554 398 321 267
259 251 190 121 28 22
SgrAI Cr'CCGG_yG
Cuts at: 0 5406 6009
Size: 5406 603
SmaI CCC'GGG
Cuts at: 0 1097 3581 3949 4036 6009
Size: 1097 2484 368 87 1973
Fragments arranged by size:
2484 1973 1097 368 87
SmlI C'TyrA_G
Cuts at: 0 4531 4960 6009
Size: 4531 429 1049
Fragments arranged by size:
4531 1049 429
SphI G_CATG'C
Cuts at: 0 438 5760 6009
Size: 438 5322 249
Fragments arranged by size:
5322 438 249
SrfI GCCC'GGGC
Cuts at: 0 4036 6009
Size: 4036 1973
StuI AGG'CCT
Cuts at: 0 409 2355 2714 2811 3299 3855 6009
Size: 409 1946 359 97 488 556 2154
Fragments arranged by size:
2154 1946 556 488 409 359 97
StyI C'CwwG_G
Cuts at: 0 261 718 1203 2103 2381 2676 2766 2937
Size: 261 457 485 900 278 295 90 171
Cuts at: 2937 3227 3524 4383 4815 5988 6009
Size: 290 297 859 432 1173 21
Fragments arranged by size:
1173 900 859 485 457 432 297 295
290 278 261 171 90 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 309 1469 3704 4135 4144 5847 6009
Size: 163 146 1160 2235 431 9 1703 162
Fragments arranged by size:
2235 1703 1160 431 163 162 146 9
TatI w'GTAC_w
Cuts at: 0 2947 3770 4797 6009
Size: 2947 823 1027 1212
Fragments arranged by size:
2947 1212 1027 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 2250 2282 3667 3699 3859 3891
Size: 1168 32 1050 32 1385 32 160 32
Cuts at: 3891 3981 4013 4177 4209 4592 4624 6009
Size: 90 32 164 32 383 32 1385
Fragments arranged by size:
1385 1385 1168 1050 383 164 160 90
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4479 5535 5589 5643 6009
Size: 4479 1056 54 54 366
Fragments arranged by size:
4479 1056 366 54 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 856 1003 1201 1705 2755 3103 5133
Size: 160 696 147 198 504 1050 348 2030
Cuts at: 5133 6009
Size: 876
Fragments arranged by size:
2030 1050 876 696 504 348 198 160
147
XmaI C'CCGG_G
Cuts at: 0 1095 3579 3947 4034 6009
Size: 1095 2484 368 87 1975
Fragments arranged by size:
2484 1975 1095 368 87
ZraI GAC'GTC
Cuts at: 0 2688 5415 6009
Size: 2688 2727 594
Fragments arranged by size:
2727 2688 594
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SalI SanDI SapI ScaI SexAI SfcI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AleI ApoI AscI AseI
AsiSI BbeI BciVI BfrBI BglII BlpI BsaBI BsiWI
BsmI BspEI BspHI BsrGI BstEII BstZ17I DraI EcoRI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NarI NdeI NotI NruI NsiI PacI PmeI PsiI
PspXI PsrI PvuI RsrII SacII SbfI SfiI SfoI
SgfI SnaBI SpeI SspI SwaI XbaI XhoI XmnI