Restriction map for
pF1KB9239
(Linear) (Six-base) MAPSORT of: KIBB9239.seq Check: 3782 from: 1 to: 4290
KIBB9239.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 280 4290
Size: 280 4010
AatII G_ACGT'C
Cuts at: 0 266 3308 3869 4290
Size: 266 3042 561 421
Fragments arranged by size:
3042 561 421 266
AccI GT'mk_AC
Cuts at: 0 1247 3869 4290
Size: 1247 2622 421
Fragments arranged by size:
2622 1247 421
AclI AA'CG_TT
Cuts at: 0 673 4290
Size: 673 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 356 705 2599 2647 3808 3819 4198
Size: 223 133 349 1894 48 1161 11 379
Cuts at: 4198 4290
Size: 92
Fragments arranged by size:
1894 1161 379 349 223 133 92 48
11
AflII C'TTAA_G
Cuts at: 0 418 4290
Size: 418 3872
AhdI GACnn_n'nnGTC
Cuts at: 0 766 4290
Size: 766 3524
AleI CACnn'nnGTG
Cuts at: 0 2894 4290
Size: 2894 1396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1469 4290
Size: 1469 2821
AlwNI CAG_nnn'CTG
Cuts at: 0 1389 1555 2049 3213 3723 3786 4290
Size: 1389 166 494 1164 510 63 504
Fragments arranged by size:
1389 1164 510 504 494 166 63
ApoI r'AATT_y
Cuts at: 0 124 352 365 517 703 883 1285 4290
Size: 124 228 13 152 186 180 402 3005
Fragments arranged by size:
3005 402 228 186 180 152 124 13
AvaI C'yCGr_G
Cuts at: 0 1206 2085 3275 4236 4290
Size: 1206 879 1190 961 54
Fragments arranged by size:
1206 1190 961 879 54
BamHI G'GATC_C
Cuts at: 0 774 2089 3464 4290
Size: 774 1315 1375 826
Fragments arranged by size:
1375 1315 826 774
BanI G'GyrC_C
Cuts at: 0 3216 3980 4120 4252 4290
Size: 3216 764 140 132 38
Fragments arranged by size:
3216 764 140 132 38
BanII G_rGCy'C
Cuts at: 0 1096 2534 3689 3715 3743 4047 4290
Size: 1096 1438 1155 26 28 304 243
Fragments arranged by size:
1438 1155 1096 304 243 28 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 765 1025 2162 4290
Size: 765 260 1137 2128
Fragments arranged by size:
2128 1137 765 260
BbvCI CC'TCA_GC
Cuts at: 0 1690 2134 4290
Size: 1690 444 2156
Fragments arranged by size:
2156 1690 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1594 1628 2274 2308 4290
Size: 1594 34 646 34 1982
Fragments arranged by size:
1982 1594 646 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 696 1995 4290
Size: 696 1299 2295
Fragments arranged by size:
2295 1299 696
BclI T'GATC_A
Cuts at: 0 3317 4290
Size: 3317 973
BfrBI ATG'CAT
Cuts at: 0 3904 4290
Size: 3904 386
BglII A'GATC_T
Cuts at: 0 1941 4290
Size: 1941 2349
BlpI GC'TnA_GC
Cuts at: 0 3035 4290
Size: 3035 1255
Bme1580I G_kGCm'C
Cuts at: 0 738 3221 3398 3780 4123 4290
Size: 738 2483 177 382 343 167
Fragments arranged by size:
2483 738 382 343 177 167
BmgBI CAC'GTC
Cuts at: 0 1272 1377 1872 2498 2982 3093 3231 4290
Size: 1272 105 495 626 484 111 138 1059
Fragments arranged by size:
1272 1059 626 495 484 138 111 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 1260 2587 4290
Size: 1260 1327 1703
Fragments arranged by size:
1703 1327 1260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1038 4290
Size: 1038 3252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 524 1352 2166 2341 2714 2946 3622
Size: 94 430 828 814 175 373 232 676
Cuts at: 3622 4290
Size: 668
Fragments arranged by size:
828 814 676 668 430 373 232 175
94
Bpu10I CC'TnA_GC
Cuts at: 0 1690 2134 3283 3709 4290
Size: 1690 444 1149 426 581
Fragments arranged by size:
1690 1149 581 444 426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 2053 2104 4290
Size: 607 1446 51 2186
Fragments arranged by size:
2186 1446 607 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 1984 3148 4290
Size: 1984 1164 1142
Fragments arranged by size:
1984 1164 1142
BsaAI yAC'GTr
Cuts at: 0 1350 1971 4290
Size: 1350 621 2319
Fragments arranged by size:
2319 1350 621
BsaBI GATnn'nnATC
Cuts at: 0 472 3064 4290
Size: 472 2592 1226
Fragments arranged by size:
2592 1226 472
BsaHI Gr'CG_yC
Cuts at: 0 263 1537 2154 3305 3866 4290
Size: 263 1274 617 1151 561 424
Fragments arranged by size:
1274 1151 617 561 424 263
BsaWI w'CCGG_w
Cuts at: 0 1877 4290
Size: 1877 2413
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 1889 1919 4290
Size: 376 30 1483 30 2371
Fragments arranged by size:
2371 1483 376 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1678 2151 2289 3028 3250 3370 3910 4290
Size: 1678 473 138 739 222 120 540 380
Fragments arranged by size:
1678 739 540 473 380 222 138 120
BseYI C'CCAG_C
Cuts at: 0 2797 3309 3716 4063 4146 4290
Size: 2797 512 407 347 83 144
Fragments arranged by size:
2797 512 407 347 144 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 373 1413 1429 1825 3004 3105 3154
Size: 220 153 1040 16 396 1179 101 49
Cuts at: 3154 3319 3666 4290
Size: 165 347 624
Fragments arranged by size:
1179 1040 624 396 347 220 165 153
101 49 16
BsiEI CG_ry'CG
Cuts at: 0 2363 2689 4290
Size: 2363 326 1601
Fragments arranged by size:
2363 1601 326
BsiHKAI G_wGCw'C
Cuts at: 0 1970 3734 4114 4290
Size: 1970 1764 380 176
Fragments arranged by size:
1970 1764 380 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1528 3598 4290
Size: 1528 2070 692
Fragments arranged by size:
2070 1528 692
Bsp1286I G_dGCh'C
Cuts at: 0 738 1096 1970 2534 3221 3398 3689 3715
Size: 738 358 874 564 687 177 291 26
Cuts at: 3715 3734 3743 3780 4047 4114 4123 4290
Size: 19 9 37 267 67 9 167
Fragments arranged by size:
874 738 687 564 358 291 267 177
167 67 37 26 19 9 9
BspHI T'CATG_A
Cuts at: 0 2672 3746 4290
Size: 2672 1074 544
Fragments arranged by size:
2672 1074 544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 280 395 1966 2057 2177 3341 4290
Size: 280 115 1571 91 120 1164 949
Fragments arranged by size:
1571 1164 949 280 120 115 91
BsrBI CCG'CTC
Cuts at: 0 186 3341 4290
Size: 186 3155 949
Fragments arranged by size:
3155 949 186
BsrDI GCAATG_nn'
Cuts at: 0 1151 1634 3029 4221 4290
Size: 1151 483 1395 1192 69
Fragments arranged by size:
1395 1192 1151 483 69
BsrFI r'CCGG_y
Cuts at: 0 43 103 155 1498 2688 3458 4290
Size: 43 60 52 1343 1190 770 832
Fragments arranged by size:
1343 1190 832 770 60 52 43
BssSI C'ACGA_G
Cuts at: 0 238 1963 4015 4290
Size: 238 1725 2052 275
Fragments arranged by size:
2052 1725 275 238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3848 4290
Size: 3848 442
BstBI TT'CG_AA
Cuts at: 0 1995 2832 4290
Size: 1995 837 1458
Fragments arranged by size:
1995 1458 837
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3813 4290
Size: 3813 477
BstYI r'GATC_y
Cuts at: 0 209 774 1941 2089 2540 3248 3464 4290
Size: 209 565 1167 148 451 708 216 826
Fragments arranged by size:
1167 826 708 565 451 216 209 148
Bsu36I CC'TnA_GG
Cuts at: 0 3022 4290
Size: 3022 1268
BtgI C'CryG_G
Cuts at: 0 1919 3149 3160 3194 3260 3296 3398 3534
Size: 1919 1230 11 34 66 36 102 136
Cuts at: 3534 3567 3594 3760 3812 4290
Size: 33 27 166 52 478
Fragments arranged by size:
1919 1230 478 166 136 102 66 52
36 34 33 27 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1013 4290
Size: 1013 3277
BtsI GCAGTG_nn'
Cuts at: 0 66 2352 4290
Size: 66 2286 1938
Fragments arranged by size:
2286 1938 66
ClaI AT'CG_AT
Cuts at: 0 1214 4290
Size: 1214 3076
DraI TTT'AAA
Cuts at: 0 522 4290
Size: 522 3768
EaeI y'GGCC_r
Cuts at: 0 1266 1365 1478 1589 1850 2079 2360 2654
Size: 1266 99 113 111 261 229 281 294
Cuts at: 2654 3005 3527 3570 3668 4218 4290
Size: 351 522 43 98 550 72
Fragments arranged by size:
1266 550 522 351 294 281 261 229
113 111 99 98 72 43
EagI C'GGCC_G
Cuts at: 0 2360 4290
Size: 2360 1930
EarI CTCTTCn'nnn_
Cuts at: 0 332 778 2023 2522 4290
Size: 332 446 1245 499 1768
Fragments arranged by size:
1768 1245 499 446 332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3490 4290
Size: 3490 800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 223 356 524 705 1352 2166 2341
Size: 94 129 133 168 181 647 814 175
Cuts at: 2341 2599 2647 2714 2946 3622 3808 3819 4198
Size: 258 48 67 232 676 186 11 379
Cuts at: 4198 4290
Size: 92
Fragments arranged by size:
814 676 647 379 258 232 186 181
175 168 133 129 94 92 67 48
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 1557 3970 4290
Size: 238 1319 2413 320
Fragments arranged by size:
2413 1319 320 238
EcoO109I rG'GnC_Cy
Cuts at: 0 638 3018 3279 4290
Size: 638 2380 261 1011
Fragments arranged by size:
2380 1011 638 261
EcoRV GAT'ATC
Cuts at: 0 935 4290
Size: 935 3355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3849 4290
Size: 3849 441
FspI TGC'GCA
Cuts at: 0 3388 4290
Size: 3388 902
FspAI rTGC'GCAy
Cuts at: 0 3388 4290
Size: 3388 902
HaeII r_GCGC'y
Cuts at: 0 1915 3169 3213 4290
Size: 1915 1254 44 1077
Fragments arranged by size:
1915 1254 1077 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 752 784 2006 2038 2356 2388 2482 2514
Size: 752 32 1222 32 318 32 94 32
Cuts at: 2514 3856 3888 4290
Size: 1342 32 402
Fragments arranged by size:
1342 1222 752 402 318 94 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 567 696 1116 3564 4290
Size: 567 129 420 2448 726
Fragments arranged by size:
2448 726 567 420 129
HindIII A'AGCT_T
Cuts at: 0 3831 4290
Size: 3831 459
HpaI GTT'AAC
Cuts at: 0 1116 4290
Size: 1116 3174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1841 2058 4290
Size: 1127 714 217 2232
Fragments arranged by size:
2232 1127 714 217
MscI TGG'CCA
Cuts at: 0 1268 1367 1480 2081 2656 3007 3529 3670
Size: 1268 99 113 601 575 351 522 141
Cuts at: 3670 4220 4290
Size: 550 70
Fragments arranged by size:
1268 601 575 550 522 351 141 113
99 70
MslI CAynn'nnrTG
Cuts at: 0 221 2894 4126 4290
Size: 221 2673 1232 164
Fragments arranged by size:
2673 1232 221 164
MspA1I CmG'CkG
Cuts at: 0 691 2547 2952 2994 3113 3290 3596 3723
Size: 691 1856 405 42 119 177 306 127
Cuts at: 3723 4250 4290
Size: 527 40
Fragments arranged by size:
1856 691 527 405 306 177 127 119
42 40
NaeI GCC'GGC
Cuts at: 0 157 1500 4290
Size: 157 1343 2790
Fragments arranged by size:
2790 1343 157
NcoI C'CATG_G
Cuts at: 0 3260 3296 3812 4290
Size: 3260 36 516 478
Fragments arranged by size:
3260 516 478 36
NgoMIV G'CCGG_C
Cuts at: 0 155 1498 4290
Size: 155 1343 2792
Fragments arranged by size:
2792 1343 155
NsiI A_TGCA'T
Cuts at: 0 3906 4290
Size: 3906 384
NspI r_CATG'y
Cuts at: 0 1754 1770 4290
Size: 1754 16 2520
Fragments arranged by size:
2520 1754 16
PasI CC'CwG_GG
Cuts at: 0 3966 4290
Size: 3966 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 652 3213 4290
Size: 652 2561 1077
Fragments arranged by size:
2561 1077 652
PfoI T'CCnGG_A
Cuts at: 0 1220 1418 2092 2923 3101 4290
Size: 1220 198 674 831 178 1189
Fragments arranged by size:
1220 1189 831 674 198 178
PmlI CAC'GTG
Cuts at: 0 1350 4290
Size: 1350 2940
PpuMI rG'GwC_Cy
Cuts at: 0 3018 3279 4290
Size: 3018 261 1011
Fragments arranged by size:
3018 1011 261
PshAI GACnn'nnGTC
Cuts at: 0 262 4290
Size: 262 4028
PspXI vC'TCGA_Gb
Cuts at: 0 4236 4290
Size: 4236 54
PstI C_TGCA'G
Cuts at: 0 1082 2054 2966 3248 4290
Size: 1082 972 912 282 1042
Fragments arranged by size:
1082 1042 972 912 282
PvuII CAG'CTG
Cuts at: 0 2547 2952 2994 3723 4290
Size: 2547 405 42 729 567
Fragments arranged by size:
2547 729 567 405 42
SacII CC_GC'GG
Cuts at: 0 3597 4290
Size: 3597 693
SapI GCTCTTCn'nnn_
Cuts at: 0 2522 4290
Size: 2522 1768
SfcI C'TryA_G
Cuts at: 0 1078 1183 2050 2962 3244 4290
Size: 1078 105 867 912 282 1046
Fragments arranged by size:
1078 1046 912 867 282 105
SgrAI Cr'CCGG_yG
Cuts at: 0 43 4290
Size: 43 4247
SmaI CCC'GGG
Cuts at: 0 3277 4290
Size: 3277 1013
SmlI C'TyrA_G
Cuts at: 0 418 586 1206 2068 2119 4236 4290
Size: 418 168 620 862 51 2117 54
Fragments arranged by size:
2117 862 620 418 168 54 51
SspI AAT'ATT
Cuts at: 0 1173 4290
Size: 1173 3117
StuI AGG'CCT
Cuts at: 0 1076 4004 4228 4290
Size: 1076 2928 224 62
Fragments arranged by size:
2928 1076 224 62
StyI C'CwwG_G
Cuts at: 0 1695 2378 3260 3296 3812 4049 4290
Size: 1695 683 882 36 516 237 241
Fragments arranged by size:
1695 882 683 516 241 237 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2674 2859 3498 4290
Size: 2674 185 639 792
Fragments arranged by size:
2674 792 639 185
TatI w'GTAC_w
Cuts at: 0 4025 4290
Size: 4025 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2074 2106 2438 2470 3628 3660 4290
Size: 2074 32 332 32 1158 32 630
Fragments arranged by size:
2074 1158 630 332 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 147 1066 1661 1873 4290
Size: 147 919 595 212 2417
Fragments arranged by size:
2417 919 595 212 147
XbaI T'CTAG_A
Cuts at: 0 2523 4290
Size: 2523 1767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2316 3888 4290
Size: 2316 1572 402
Fragments arranged by size:
2316 1572 402
XhoI C'TCGA_G
Cuts at: 0 1206 4236 4290
Size: 1206 3030 54
Fragments arranged by size:
3030 1206 54
XmaI C'CCGG_G
Cuts at: 0 3275 4290
Size: 3275 1015
XmnI GAAnn'nnTTC
Cuts at: 0 3083 4290
Size: 3083 1207
ZraI GAC'GTC
Cuts at: 0 264 3306 3867 4290
Size: 264 3042 561 423
Fragments arranged by size:
3042 561 423 264
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AhdI AleI
AlfI AlwNI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspXI PstI PvuII SacII SapI SfcI
SgrAI SmaI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflIII AgeI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BglI BmtI BsiWI
BsmI BspEI BsrGI BssHII BstEII BstZ17I CspCI DraIII
DrdI EcoICRI EcoRI FseI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI PacI PciI PmeI
PpiI PsiI PspOMI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI
SpeI SphI SrfI SwaI