(Linear) (Six-base) MAPSORT of: KIBB9261.seq Check: 284 from: 1 to: 612 KIBB9261.orf With 182 enzymes: SgfI * October 7, 2009 14:26 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 423 612 Size: 423 189 AfeI AGC'GCT Cuts at: 0 23 583 612 Size: 23 560 29 Fragments arranged by size: 560 29 23 ApaI G_GGCC'C Cuts at: 0 9 612 Size: 9 603 AvaI C'yCGr_G Cuts at: 0 389 612 Size: 389 223 BanI G'GyrC_C Cuts at: 0 28 67 412 458 612 Size: 28 39 345 46 154 Fragments arranged by size: 345 154 46 39 28 BanII G_rGCy'C Cuts at: 0 9 612 Size: 9 603 BbeI G_GCGC'C Cuts at: 0 32 71 612 Size: 32 39 541 Fragments arranged by size: 541 39 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 317 612 Size: 317 295 BbvCI CC'TCA_GC Cuts at: 0 574 612 Size: 574 38 Bme1580I G_kGCm'C Cuts at: 0 9 612 Size: 9 603 BmgBI CAC'GTC Cuts at: 0 108 171 612 Size: 108 63 441 Fragments arranged by size: 441 108 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 612 Size: 241 371 Bpu10I CC'TnA_GC Cuts at: 0 574 612 Size: 574 38 BsaHI Gr'CG_yC Cuts at: 0 29 68 612 Size: 29 39 544 Fragments arranged by size: 544 39 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 94 124 214 244 612 Size: 94 30 90 30 368 Fragments arranged by size: 368 94 90 30 30 BseYI C'CCAG_C Cuts at: 0 24 612 Size: 24 588 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 612 Size: 508 104 BsmBI CGTCTCn'nnnn_ Cuts at: 0 114 612 Size: 114 498 Bsp1286I G_dGCh'C Cuts at: 0 9 612 Size: 9 603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 488 612 Size: 488 124 BsrBI CCG'CTC Cuts at: 0 12 612 Size: 12 600 BsrFI r'CCGG_y Cuts at: 0 82 612 Size: 82 530 BstYI r'GATC_y Cuts at: 0 224 612 Size: 224 388 BtgI C'CryG_G Cuts at: 0 192 401 612 Size: 192 209 211 Fragments arranged by size: 211 209 192 DrdI GACnn_nn'nnGTC Cuts at: 0 106 612 Size: 106 506 EaeI y'GGCC_r Cuts at: 0 84 114 612 Size: 84 30 498 Fragments arranged by size: 498 84 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 423 612 Size: 241 182 189 Fragments arranged by size: 241 189 182 EcoO109I rG'GnC_Cy Cuts at: 0 5 6 612 Size: 5 1 606 Fragments arranged by size: 606 5 1 HaeII r_GCGC'y Cuts at: 0 25 32 71 585 612 Size: 25 7 39 514 27 Fragments arranged by size: 514 39 27 25 7 KasI G'GCGC_C Cuts at: 0 28 67 612 Size: 28 39 545 Fragments arranged by size: 545 39 28 MspA1I CmG'CkG Cuts at: 0 194 200 612 Size: 194 6 412 Fragments arranged by size: 412 194 6 NaeI GCC'GGC Cuts at: 0 84 612 Size: 84 528 NarI GG'CG_CC Cuts at: 0 29 68 612 Size: 29 39 544 Fragments arranged by size: 544 39 29 NgoMIV G'CCGG_C Cuts at: 0 82 612 Size: 82 530 PasI CC'CwG_GG Cuts at: 0 95 612 Size: 95 517 PflMI CCAn_nnn'nTGG Cuts at: 0 42 612 Size: 42 570 PspOMI G'GGCC_C Cuts at: 0 5 612 Size: 5 607 PstI C_TGCA'G Cuts at: 0 607 612 Size: 607 5 SacII CC_GC'GG Cuts at: 0 195 612 Size: 195 417 SfcI C'TryA_G Cuts at: 0 603 612 Size: 603 9 SfoI GGC'GCC Cuts at: 0 30 69 612 Size: 30 39 543 Fragments arranged by size: 543 39 30 SmaI CCC'GGG Cuts at: 0 391 612 Size: 391 221 StuI AGG'CCT Cuts at: 0 475 601 612 Size: 475 126 11 Fragments arranged by size: 475 126 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 277 612 Size: 277 335 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 214 246 612 Size: 214 32 366 Fragments arranged by size: 366 214 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 339 612 Size: 339 273 XmaI C'CCGG_G Cuts at: 0 389 612 Size: 389 223 Enzymes that do cut: AcuI AfeI ApaI AvaI BanI BanII BbeI BbsI BbvCI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaXI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI DrdI EaeI Eco57MI EcoO109I HaeII KasI MspA1I NaeI NarI NgoMIV PasI PflMI PspOMI PstI SacII SfcI SfoI SmaI StuI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI Tth111I XbaI XhoI XmnI ZraI