Restriction map for
pF1KB9271
(Linear) (Six-base) MAPSORT of: KIBB9271.seq Check: 20 from: 1 to: 1764
KIBB9271.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 229 241 1764
Size: 229 12 1523
Fragments arranged by size:
1523 229 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1698 1764
Size: 1698 66
AflII C'TTAA_G
Cuts at: 0 492 1764
Size: 492 1272
AleI CACnn'nnGTG
Cuts at: 0 1337 1764
Size: 1337 427
AlwNI CAG_nnn'CTG
Cuts at: 0 198 1764
Size: 198 1566
ApaI G_GGCC'C
Cuts at: 0 639 1764
Size: 639 1125
ApoI r'AATT_y
Cuts at: 0 386 1560 1764
Size: 386 1174 204
Fragments arranged by size:
1174 386 204
AvaI C'yCGr_G
Cuts at: 0 917 1764
Size: 917 847
BamHI G'GATC_C
Cuts at: 0 1580 1764
Size: 1580 184
BanI G'GyrC_C
Cuts at: 0 6 71 1764
Size: 6 65 1693
Fragments arranged by size:
1693 65 6
BanII G_rGCy'C
Cuts at: 0 639 1764
Size: 639 1125
BbeI G_GCGC'C
Cuts at: 0 10 1764
Size: 10 1754
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 1764
Size: 41 1723
Bme1580I G_kGCm'C
Cuts at: 0 74 639 1764
Size: 74 565 1125
Fragments arranged by size:
1125 565 74
BmgBI CAC'GTC
Cuts at: 0 39 1764
Size: 39 1725
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 758 1764
Size: 151 607 1006
Fragments arranged by size:
1006 607 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 321 561 1764
Size: 321 240 1203
Fragments arranged by size:
1203 321 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 136 686 1190 1526 1610 1694 1764
Size: 77 59 550 504 336 84 84 70
Fragments arranged by size:
550 504 336 84 84 77 70 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 423 533 1764
Size: 321 102 110 1231
Fragments arranged by size:
1231 321 110 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 141 1764
Size: 112 29 1623
Fragments arranged by size:
1623 112 29
BsaHI Gr'CG_yC
Cuts at: 0 7 1764
Size: 7 1757
BsaWI w'CCGG_w
Cuts at: 0 832 1252 1764
Size: 832 420 512
Fragments arranged by size:
832 512 420
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 952 982 1764
Size: 952 30 782
Fragments arranged by size:
952 782 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 960 1764
Size: 960 804
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1377 1764
Size: 1377 387
BsiHKAI G_wGCw'C
Cuts at: 0 818 1764
Size: 818 946
BsmI GAATG_Cn'
Cuts at: 0 871 1612 1696 1764
Size: 871 741 84 68
Fragments arranged by size:
871 741 84 68
Bsp1286I G_dGCh'C
Cuts at: 0 74 639 818 1764
Size: 74 565 179 946
Fragments arranged by size:
946 565 179 74
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 130 229 241 788 1563 1764
Size: 130 99 12 547 775 201
Fragments arranged by size:
775 547 201 130 99 12
BsrBI CCG'CTC
Cuts at: 0 884 1052 1764
Size: 884 168 712
Fragments arranged by size:
884 712 168
BsrDI GCAATG_nn'
Cuts at: 0 693 1701 1764
Size: 693 1008 63
Fragments arranged by size:
1008 693 63
BsrFI r'CCGG_y
Cuts at: 0 9 1764
Size: 9 1755
BstYI r'GATC_y
Cuts at: 0 331 1580 1764
Size: 331 1249 184
Fragments arranged by size:
1249 331 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1201 1764
Size: 1201 563
BtsI GCAGTG_nn'
Cuts at: 0 1365 1764
Size: 1365 399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 675 710 843 878 927 962 1263 1298
Size: 675 35 133 35 49 35 301 35
Cuts at: 1298 1683 1718 1764
Size: 385 35 46
Fragments arranged by size:
675 385 301 133 49 46 35 35
35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1074 1764
Size: 1074 690
EarI CTCTTCn'nnn_
Cuts at: 0 205 891 1311 1479 1764
Size: 205 686 420 168 285
Fragments arranged by size:
686 420 285 205 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 136 686 1190 1526 1610 1694 1698
Size: 77 59 550 504 336 84 84 4
Cuts at: 1698 1764
Size: 66
Fragments arranged by size:
550 504 336 84 84 77 66 59
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1764
Size: 100 1664
EcoO109I rG'GnC_Cy
Cuts at: 0 92 636 1244 1412 1764
Size: 92 544 608 168 352
Fragments arranged by size:
608 544 352 168 92
FspI TGC'GCA
Cuts at: 0 237 1764
Size: 237 1527
HaeII r_GCGC'y
Cuts at: 0 10 1764
Size: 10 1754
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 900 932 1764
Size: 900 32 832
Fragments arranged by size:
900 832 32
HindIII A'AGCT_T
Cuts at: 0 953 1764
Size: 953 811
KasI G'GCGC_C
Cuts at: 0 6 1764
Size: 6 1758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1257 1764
Size: 1257 507
MslI CAynn'nnrTG
Cuts at: 0 539 765 1101 1337 1764
Size: 539 226 336 236 427
Fragments arranged by size:
539 427 336 236 226
MspA1I CmG'CkG
Cuts at: 0 285 1764
Size: 285 1479
NaeI GCC'GGC
Cuts at: 0 11 1764
Size: 11 1753
NarI GG'CG_CC
Cuts at: 0 7 1764
Size: 7 1757
NdeI CA'TA_TG
Cuts at: 0 734 986 1322 1764
Size: 734 252 336 442
Fragments arranged by size:
734 442 336 252
NgoMIV G'CCGG_C
Cuts at: 0 9 1764
Size: 9 1755
NspI r_CATG'y
Cuts at: 0 602 1764
Size: 602 1162
PfoI T'CCnGG_A
Cuts at: 0 311 1764
Size: 311 1453
PpuMI rG'GwC_Cy
Cuts at: 0 92 1244 1412 1764
Size: 92 1152 168 352
Fragments arranged by size:
1152 352 168 92
PsiI TTA'TAA
Cuts at: 0 455 1764
Size: 455 1309
PspOMI G'GGCC_C
Cuts at: 0 635 1764
Size: 635 1129
PstI C_TGCA'G
Cuts at: 0 127 1764
Size: 127 1637
PvuII CAG'CTG
Cuts at: 0 285 1764
Size: 285 1479
SapI GCTCTTCn'nnn_
Cuts at: 0 891 1311 1479 1764
Size: 891 420 168 285
Fragments arranged by size:
891 420 285 168
SbfI CC_TGCA'GG
Cuts at: 0 127 1764
Size: 127 1637
SfcI C'TryA_G
Cuts at: 0 123 963 1467 1764
Size: 123 840 504 297
Fragments arranged by size:
840 504 297 123
SfoI GGC'GCC
Cuts at: 0 8 1764
Size: 8 1756
SgrAI Cr'CCGG_yG
Cuts at: 0 9 1764
Size: 9 1755
SmlI C'TyrA_G
Cuts at: 0 336 402 492 548 917 1764
Size: 336 66 90 56 369 847
Fragments arranged by size:
847 369 336 90 66 56
StuI AGG'CCT
Cuts at: 0 218 1039 1207 1291 1764
Size: 218 821 168 84 473
Fragments arranged by size:
821 473 218 168 84
StyI C'CwwG_G
Cuts at: 0 1433 1764
Size: 1433 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 892 1764
Size: 892 872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1404 1436 1764
Size: 1404 32 328
Fragments arranged by size:
1404 328 32
XhoI C'TCGA_G
Cuts at: 0 917 1764
Size: 917 847
XmnI GAAnn'nnTTC
Cuts at: 0 787 1627 1764
Size: 787 840 137
Fragments arranged by size:
840 787 137
Enzymes that do cut:
AarI AcuI AflII AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstYI BtgZI BtsI CspCI DraIII EarI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HindIII KasI MmeI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PfoI PpuMI
PsiI PspOMI PstI PvuII SapI SbfI SfcI SfoI
SgrAI SmlI StuI StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI Bpu10I BsaAI BsaBI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI DraI DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HincII HpaI KpnI MfeI MluI MscI NcoI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI ScaI SexAI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XmaI ZraI