(Linear) (Six-base) MAPSORT of: KIBB9295.seq  Check: 7136  from: 1  to: 609



KIBB9295.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     563     609 
   Size:        305     258      46

  Fragments arranged by size: 

                 305     258      46
 
AlwNI CAG_nnn'CTG

Cuts at:      0     328     609 
   Size:        328     281
 
BanI G'GyrC_C

Cuts at:      0     148     609 
   Size:        148     461
 
BanII G_rGCy'C

Cuts at:      0     319     609 
   Size:        319     290
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36     609 
   Size:         36     573
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     140     609 
   Size:        140     469
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     470     609 
   Size:        101     369     139

  Fragments arranged by size: 

                 369     139     101
 
BsiHKAI G_wGCw'C

Cuts at:      0     319     609 
   Size:        319     290
 
Bsp1286I G_dGCh'C

Cuts at:      0     319     609 
   Size:        319     290
 
BsrFI r'CCGG_y

Cuts at:      0      75     609 
   Size:         75     534
 
BtgI C'CryG_G

Cuts at:      0      10     609 
   Size:         10     599
 
BtsI GCAGTG_nn'

Cuts at:      0     369     609 
   Size:        369     240
 
EaeI y'GGCC_r

Cuts at:      0      73     609 
   Size:         73     536
 
EarI CTCTTCn'nnn_

Cuts at:      0     324     512     609 
   Size:        324     188      97

  Fragments arranged by size: 

                 324     188      97
 
EcoICRI GAG'CTC

Cuts at:      0     317     609 
   Size:        317     292
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     305     470     563     609 
   Size:        101     204     165      93      46

  Fragments arranged by size: 

                 204     165     101      93      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     172     609 
   Size:        172     437
 
FseI GG_CCGG'CC

Cuts at:      0      79     609 
   Size:         79     530
 
HaeII r_GCGC'y

Cuts at:      0     107     609 
   Size:        107     502
 
MspA1I CmG'CkG

Cuts at:      0      12     609 
   Size:         12     597
 
NaeI GCC'GGC

Cuts at:      0      77     609 
   Size:         77     532
 
NgoMIV G'CCGG_C

Cuts at:      0      75     609 
   Size:         75     534
 
PfoI T'CCnGG_A

Cuts at:      0     475     486     609 
   Size:        475      11     123

  Fragments arranged by size: 

                 475     123      11
 
PpuMI rG'GwC_Cy

Cuts at:      0     172     609 
   Size:        172     437
 
PstI C_TGCA'G

Cuts at:      0     354     609 
   Size:        354     255
 
SacI G_AGCT'C

Cuts at:      0     319     609 
   Size:        319     290
 
SacII CC_GC'GG

Cuts at:      0      13     609 
   Size:         13     596
 
SapI GCTCTTCn'nnn_

Cuts at:      0     324     609 
   Size:        324     285
 
SexAI A'CCwGG_T

Cuts at:      0      38     609 
   Size:         38     571
 
SfcI C'TryA_G

Cuts at:      0     350     609 
   Size:        350     259
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166     609 
   Size:        166     443
 
TatI w'GTAC_w

Cuts at:      0      23     200     609 
   Size:         23     177     409

  Fragments arranged by size: 

                 409     177      23
 
Tth111I GACn'n_nGTC

Cuts at:      0      40     609 
   Size:         40     569
 

 Enzymes that do cut:

     AcuI    AlwNI     BanI    BanII     BbsI    BciVI     BpmI  BsiHKAI
 Bsp1286I    BsrFI     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I     FseI    HaeII   MspA1I     NaeI   NgoMIV     PfoI    PpuMI
     PstI     SacI    SacII     SapI    SexAI     SfcI    TaqII     TatI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI