Restriction map for
pF1KB9297
(Linear) (Six-base) MAPSORT of: KIBB9297.seq Check: 8030 from: 1 to: 942
KIBB9297.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 525 942
Size: 525 417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 942
Size: 49 893
AflIII A'CryG_T
Cuts at: 0 620 942
Size: 620 322
AlwNI CAG_nnn'CTG
Cuts at: 0 309 942
Size: 309 633
ApaI G_GGCC'C
Cuts at: 0 325 942
Size: 325 617
ApoI r'AATT_y
Cuts at: 0 798 907 942
Size: 798 109 35
Fragments arranged by size:
798 109 35
AseI AT'TA_AT
Cuts at: 0 793 942
Size: 793 149
AvaI C'yCGr_G
Cuts at: 0 281 318 916 942
Size: 281 37 598 26
Fragments arranged by size:
598 281 37 26
BanII G_rGCy'C
Cuts at: 0 325 502 942
Size: 325 177 440
Fragments arranged by size:
440 325 177
BbsI GAAGACnn'nnnn_
Cuts at: 0 57 942
Size: 57 885
BclI T'GATC_A
Cuts at: 0 71 942
Size: 71 871
BglII A'GATC_T
Cuts at: 0 650 942
Size: 650 292
Bme1580I G_kGCm'C
Cuts at: 0 325 942
Size: 325 617
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 942
Size: 288 654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 331 942
Size: 40 291 611
Fragments arranged by size:
611 291 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 942
Size: 208 734
BsaAI yAC'GTr
Cuts at: 0 623 942
Size: 623 319
BsaBI GATnn'nnATC
Cuts at: 0 469 698 942
Size: 469 229 244
Fragments arranged by size:
469 244 229
BsaWI w'CCGG_w
Cuts at: 0 252 942
Size: 252 690
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 234 264 942
Size: 234 30 678
Fragments arranged by size:
678 234 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 942
Size: 475 467
BsiHKAI G_wGCw'C
Cuts at: 0 502 942
Size: 502 440
Bsp1286I G_dGCh'C
Cuts at: 0 325 502 942
Size: 325 177 440
Fragments arranged by size:
440 325 177
BspEI T'CCGG_A
Cuts at: 0 252 942
Size: 252 690
BspHI T'CATG_A
Cuts at: 0 68 942
Size: 68 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 525 942
Size: 525 417
BsrGI T'GTAC_A
Cuts at: 0 35 594 942
Size: 35 559 348
Fragments arranged by size:
559 348 35
BssSI C'ACGA_G
Cuts at: 0 457 942
Size: 457 485
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 942
Size: 810 132
BstYI r'GATC_y
Cuts at: 0 650 942
Size: 650 292
BtgI C'CryG_G
Cuts at: 0 86 519 942
Size: 86 433 423
Fragments arranged by size:
433 423 86
DraI TTT'AAA
Cuts at: 0 749 942
Size: 749 193
EaeI y'GGCC_r
Cuts at: 0 89 566 942
Size: 89 477 376
Fragments arranged by size:
477 376 89
EcoICRI GAG'CTC
Cuts at: 0 500 942
Size: 500 442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 49 331 942
Size: 40 9 282 611
Fragments arranged by size:
611 282 40 9
EcoO109I rG'GnC_Cy
Cuts at: 0 306 942
Size: 306 636
HaeII r_GCGC'y
Cuts at: 0 86 942
Size: 86 856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 942
Size: 124 32 786
Fragments arranged by size:
786 124 32
HindIII A'AGCT_T
Cuts at: 0 757 942
Size: 757 185
MscI TGG'CCA
Cuts at: 0 91 942
Size: 91 851
MspA1I CmG'CkG
Cuts at: 0 272 942
Size: 272 670
NcoI C'CATG_G
Cuts at: 0 86 942
Size: 86 856
PasI CC'CwG_GG
Cuts at: 0 108 574 942
Size: 108 466 368
Fragments arranged by size:
466 368 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 641 942
Size: 309 332 301
Fragments arranged by size:
332 309 301
PsiI TTA'TAA
Cuts at: 0 716 942
Size: 716 226
PspOMI G'GGCC_C
Cuts at: 0 321 942
Size: 321 621
SacI G_AGCT'C
Cuts at: 0 502 942
Size: 502 440
SfcI C'TryA_G
Cuts at: 0 488 942
Size: 488 454
SmlI C'TyrA_G
Cuts at: 0 223 942
Size: 223 719
StyI C'CwwG_G
Cuts at: 0 86 942
Size: 86 856
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 415 942
Size: 307 108 527
Fragments arranged by size:
527 307 108
TatI w'GTAC_w
Cuts at: 0 35 259 407 594 942
Size: 35 224 148 187 348
Fragments arranged by size:
348 224 187 148 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 234 266 942
Size: 234 32 676
Fragments arranged by size:
676 234 32
XmnI GAAnn'nnTTC
Cuts at: 0 373 942
Size: 373 569
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApoI AseI AvaI
BanII BbsI BclI BglII Bme1580I BmrI BpmI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrGI BssSI BstAPI BstYI BtgI DraI
EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII MscI
MspA1I NcoI PasI PflMI PsiI PspOMI SacI SfcI
SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaHI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI