(Linear) (Six-base) MAPSORT of: KIBB9348.seq Check: 9815 from: 1 to: 819 KIBB9348.orf With 182 enzymes: SgfI * February 28, 2007 11:28 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 819 Size: 568 251 AhdI GACnn_n'nnGTC Cuts at: 0 416 819 Size: 416 403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 548 819 Size: 548 271 ApaI G_GGCC'C Cuts at: 0 815 819 Size: 815 4 BanI G'GyrC_C Cuts at: 0 671 819 Size: 671 148 BanII G_rGCy'C Cuts at: 0 509 815 819 Size: 509 306 4 Fragments arranged by size: 509 306 4 BbeI G_GCGC'C Cuts at: 0 675 819 Size: 675 144 BbvCI CC'TCA_GC Cuts at: 0 536 819 Size: 536 283 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 765 799 819 Size: 765 34 20 Fragments arranged by size: 765 34 20 BclI T'GATC_A Cuts at: 0 563 819 Size: 563 256 Bme1580I G_kGCm'C Cuts at: 0 815 819 Size: 815 4 BmrI ACTGGGnnnn_n' Cuts at: 0 410 819 Size: 410 409 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 428 819 Size: 428 391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 112 217 247 415 471 733 819 Size: 52 60 105 30 168 56 262 86 Fragments arranged by size: 262 168 105 86 60 56 52 30 Bpu10I CC'TnA_GC Cuts at: 0 536 819 Size: 536 283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 819 Size: 4 815 BsaI GGTCTCn'nnnn_ Cuts at: 0 793 819 Size: 793 26 BsaBI GATnn'nnATC Cuts at: 0 694 819 Size: 694 125 BsaHI Gr'CG_yC Cuts at: 0 672 819 Size: 672 147 BsaWI w'CCGG_w Cuts at: 0 462 819 Size: 462 357 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 109 330 553 819 Size: 109 221 223 266 Fragments arranged by size: 266 223 221 109 BseYI C'CCAG_C Cuts at: 0 554 819 Size: 554 265 BsiHKAI G_wGCw'C Cuts at: 0 38 485 509 819 Size: 38 447 24 310 Fragments arranged by size: 447 310 38 24 Bsp1286I G_dGCh'C Cuts at: 0 38 485 509 815 819 Size: 38 447 24 306 4 Fragments arranged by size: 447 306 38 24 4 BspEI T'CCGG_A Cuts at: 0 462 819 Size: 462 357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 278 819 Size: 278 541 BstAPI GCAn_nnn'nTGC Cuts at: 0 42 819 Size: 42 777 BstXI CCAn_nnnn'nTGG Cuts at: 0 123 155 258 438 819 Size: 123 32 103 180 381 Fragments arranged by size: 381 180 123 103 32 Bsu36I CC'TnA_GG Cuts at: 0 11 819 Size: 11 808 BtgI C'CryG_G Cuts at: 0 122 819 Size: 122 697 BtsI GCAGTG_nn' Cuts at: 0 344 819 Size: 344 475 DrdI GACnn_nn'nnGTC Cuts at: 0 786 819 Size: 786 33 EaeI y'GGCC_r Cuts at: 0 49 746 819 Size: 49 697 73 Fragments arranged by size: 697 73 49 EarI CTCTTCn'nnn_ Cuts at: 0 52 321 514 819 Size: 52 269 193 305 Fragments arranged by size: 305 269 193 52 EcoICRI GAG'CTC Cuts at: 0 507 819 Size: 507 312 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 112 217 247 415 471 568 733 Size: 52 60 105 30 168 56 97 165 Cuts at: 733 819 Size: 86 Fragments arranged by size: 168 165 105 97 86 60 56 52 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 397 715 819 Size: 397 318 104 Fragments arranged by size: 397 318 104 EcoO109I rG'GnC_Cy Cuts at: 0 15 543 812 819 Size: 15 528 269 7 Fragments arranged by size: 528 269 15 7 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 600 819 Size: 600 219 HaeII r_GCGC'y Cuts at: 0 675 819 Size: 675 144 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 169 201 402 434 819 Size: 169 32 201 32 385 Fragments arranged by size: 385 201 169 32 32 KasI G'GCGC_C Cuts at: 0 671 819 Size: 671 148 MfeI C'AATT_G Cuts at: 0 432 819 Size: 432 387 MscI TGG'CCA Cuts at: 0 51 819 Size: 51 768 NarI GG'CG_CC Cuts at: 0 672 819 Size: 672 147 NcoI C'CATG_G Cuts at: 0 122 819 Size: 122 697 NdeI CA'TA_TG Cuts at: 0 174 819 Size: 174 645 NspI r_CATG'y Cuts at: 0 9 819 Size: 9 810 PflMI CCAn_nnn'nTGG Cuts at: 0 178 819 Size: 178 641 PfoI T'CCnGG_A Cuts at: 0 710 819 Size: 710 109 PpuMI rG'GwC_Cy Cuts at: 0 15 543 819 Size: 15 528 276 Fragments arranged by size: 528 276 15 PspOMI G'GGCC_C Cuts at: 0 811 819 Size: 811 8 SacI G_AGCT'C Cuts at: 0 509 819 Size: 509 310 SapI GCTCTTCn'nnn_ Cuts at: 0 321 514 819 Size: 321 193 305 Fragments arranged by size: 321 305 193 ScaI AGT'ACT Cuts at: 0 616 819 Size: 616 203 SexAI A'CCwGG_T Cuts at: 0 476 819 Size: 476 343 SfcI C'TryA_G Cuts at: 0 281 819 Size: 281 538 SfoI GGC'GCC Cuts at: 0 673 819 Size: 673 146 SmlI C'TyrA_G Cuts at: 0 19 819 Size: 19 800 StyI C'CwwG_G Cuts at: 0 46 122 819 Size: 46 76 697 Fragments arranged by size: 697 76 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 165 268 819 Size: 165 103 551 Fragments arranged by size: 551 165 103 TatI w'GTAC_w Cuts at: 0 200 614 819 Size: 200 414 205 Fragments arranged by size: 414 205 200 Enzymes that do cut: AcuI AhdI AlfI ApaI BanI BanII BbeI BbvCI BcgI BclI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BstAPI BstXI Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MfeI MscI NarI NcoI NdeI NspI PflMI PfoI PpuMI PspOMI SacI SapI ScaI SexAI SfcI SfoI SmlI StyI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BsaAI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MluI MmeI MslI MspA1I NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI