Restriction map for
pF1KB9351
(Linear) (Six-base) MAPSORT of: KIBB9351.seq Check: 6575 from: 1 to: 5832
KIBB9351.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5705 5832
Size: 5705 127
AatII G_ACGT'C
Cuts at: 0 2940 5832
Size: 2940 2892
AccI GT'mk_AC
Cuts at: 0 1008 3353 4385 5669 5832
Size: 1008 2345 1032 1284 163
Fragments arranged by size:
2345 1284 1032 1008 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 2180 2575 2996 3106 3454 5204 5300
Size: 166 2014 395 421 110 348 1750 96
Cuts at: 5300 5832
Size: 532
Fragments arranged by size:
2014 1750 532 421 395 348 166 110
96
AfeI AGC'GCT
Cuts at: 0 576 5832
Size: 576 5256
AflII C'TTAA_G
Cuts at: 0 2317 5832
Size: 2317 3515
AflIII A'CryG_T
Cuts at: 0 1858 4380 5832
Size: 1858 2522 1452
Fragments arranged by size:
2522 1858 1452
AgeI A'CCGG_T
Cuts at: 0 4796 5832
Size: 4796 1036
AhdI GACnn_n'nnGTC
Cuts at: 0 2617 3873 5832
Size: 2617 1256 1959
Fragments arranged by size:
2617 1959 1256
AleI CACnn'nnGTG
Cuts at: 0 1504 2885 5832
Size: 1504 1381 2947
Fragments arranged by size:
2947 1504 1381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3637 4419 4487 5832
Size: 3637 782 68 1345
Fragments arranged by size:
3637 1345 782 68
AlwNI CAG_nnn'CTG
Cuts at: 0 136 2917 3025 5697 5832
Size: 136 2781 108 2672 135
Fragments arranged by size:
2781 2672 136 135 108
ApaLI G'TGCA_C
Cuts at: 0 1078 2460 5547 5602 5832
Size: 1078 1382 3087 55 230
Fragments arranged by size:
3087 1382 1078 230 55
ApoI r'AATT_y
Cuts at: 0 55 1269 1971 2037 2133 2345 4204 4263
Size: 55 1214 702 66 96 212 1859 59
Cuts at: 4263 4356 4468 4731 5297 5527 5749 5832
Size: 93 112 263 566 230 222 83
Fragments arranged by size:
1859 1214 702 566 263 230 222 212
112 96 93 83 66 59 55
AseI AT'TA_AT
Cuts at: 0 3076 5832
Size: 3076 2756
AvaI C'yCGr_G
Cuts at: 0 5189 5832
Size: 5189 643
BamHI G'GATC_C
Cuts at: 0 175 5832
Size: 175 5657
BanI G'GyrC_C
Cuts at: 0 3548 3652 4820 5832
Size: 3548 104 1168 1012
Fragments arranged by size:
3548 1168 1012 104
BanII G_rGCy'C
Cuts at: 0 3170 4167 4847 5513 5832
Size: 3170 997 680 666 319
Fragments arranged by size:
3170 997 680 666 319
BbsI GAAGACnn'nnnn_
Cuts at: 0 140 5400 5832
Size: 140 5260 432
Fragments arranged by size:
5260 432 140
BbvCI CC'TCA_GC
Cuts at: 0 5614 5832
Size: 5614 218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 62 96 4597 4631 5832
Size: 62 34 4501 34 1201
Fragments arranged by size:
4501 1201 62 34 34
BfrBI ATG'CAT
Cuts at: 0 449 483 2493 4020 5832
Size: 449 34 2010 1527 1812
Fragments arranged by size:
2010 1812 1527 449 34
BglII A'GATC_T
Cuts at: 0 2176 4141 5832
Size: 2176 1965 1691
Fragments arranged by size:
2176 1965 1691
BlpI GC'TnA_GC
Cuts at: 0 2022 5644 5832
Size: 2022 3622 188
Fragments arranged by size:
3622 2022 188
Bme1580I G_kGCm'C
Cuts at: 0 1082 2464 3333 3551 4761 4921 5551 5606
Size: 1082 1382 869 218 1210 160 630 55
Cuts at: 5606 5832
Size: 226
Fragments arranged by size:
1382 1210 1082 869 630 226 218 160
55
BmrI ACTGGGnnnn_n'
Cuts at: 0 998 1577 1660 1946 2127 3332 5028 5832
Size: 998 579 83 286 181 1205 1696 804
Fragments arranged by size:
1696 1205 998 804 579 286 181 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3897 5810 5832
Size: 3897 1913 22
Fragments arranged by size:
3897 1913 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1333 1925 4792 5832
Size: 581 752 592 2867 1040
Fragments arranged by size:
2867 1040 752 592 581
Bpu10I CC'TnA_GC
Cuts at: 0 3885 3965 4216 4587 5501 5614 5832
Size: 3885 80 251 371 914 113 218
Fragments arranged by size:
3885 914 371 251 218 113 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2643 5832
Size: 2643 3189
BsaI GGTCTCn'nnnn_
Cuts at: 0 68 5338 5832
Size: 68 5270 494
Fragments arranged by size:
5270 494 68
BsaHI Gr'CG_yC
Cuts at: 0 2937 5832
Size: 2937 2895
BsaWI w'CCGG_w
Cuts at: 0 812 4796 5832
Size: 812 3984 1036
Fragments arranged by size:
3984 1036 812
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 242 272 2305 2335 5832
Size: 242 30 2033 30 3497
Fragments arranged by size:
3497 2033 242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 957 2326 3043 3326 4718 5832
Size: 957 1369 717 283 1392 1114
Fragments arranged by size:
1392 1369 1114 957 717 283
BseYI C'CCAG_C
Cuts at: 0 1343 1391 3398 4776 5832
Size: 1343 48 2007 1378 1056
Fragments arranged by size:
2007 1378 1343 1056 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 3121 3175 3846 4484 5832
Size: 625 2496 54 671 638 1348
Fragments arranged by size:
2496 1348 671 638 625 54
BsiHKAI G_wGCw'C
Cuts at: 0 1059 1082 1941 2464 2996 3170 4345 4726
Size: 1059 23 859 523 532 174 1175 381
Cuts at: 4726 5551 5606 5725 5832
Size: 825 55 119 107
Fragments arranged by size:
1175 1059 859 825 532 523 381 174
119 107 55 23
BsmI GAATG_Cn'
Cuts at: 0 795 2573 2754 3686 4022 5218 5832
Size: 795 1778 181 932 336 1196 614
Fragments arranged by size:
1778 1196 932 795 614 336 181
Bsp1286I G_dGCh'C
Cuts at: 0 1059 1082 1941 2464 2996 3170 3333 3551
Size: 1059 23 859 523 532 174 163 218
Cuts at: 3551 4167 4345 4726 4761 4847 4921 5513 5551
Size: 616 178 381 35 86 74 592 38
Cuts at: 5551 5606 5725 5832
Size: 55 119 107
Fragments arranged by size:
1059 859 616 592 532 523 381 218
178 174 163 119 107 86 74 55
38 35 23
BspEI T'CCGG_A
Cuts at: 0 812 5832
Size: 812 5020
BspHI T'CATG_A
Cuts at: 0 1698 1991 5832
Size: 1698 293 3841
Fragments arranged by size:
3841 1698 293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2286 5705 5832
Size: 2286 3419 127
Fragments arranged by size:
3419 2286 127
BsrBI CCG'CTC
Cuts at: 0 4747 5484 5832
Size: 4747 737 348
Fragments arranged by size:
4747 737 348
BsrDI GCAATG_nn'
Cuts at: 0 438 734 2069 3295 4290 4739 5381 5832
Size: 438 296 1335 1226 995 449 642 451
Fragments arranged by size:
1335 1226 995 642 451 449 438 296
BsrFI r'CCGG_y
Cuts at: 0 4796 5832
Size: 4796 1036
BsrGI T'GTAC_A
Cuts at: 0 2636 5832
Size: 2636 3196
BssSI C'ACGA_G
Cuts at: 0 199 3061 3178 5832
Size: 199 2862 117 2654
Fragments arranged by size:
2862 2654 199 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1432 3108 3914 4507 5832
Size: 1432 1676 806 593 1325
Fragments arranged by size:
1676 1432 1325 806 593
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1738 3726 4077 4578 5018 5141 5832
Size: 1738 1988 351 501 440 123 691
Fragments arranged by size:
1988 1738 691 501 440 351 123
BstYI r'GATC_y
Cuts at: 0 175 1764 2104 2176 2943 4141 5130 5304
Size: 175 1589 340 72 767 1198 989 174
Cuts at: 5304 5832
Size: 528
Fragments arranged by size:
1589 1198 989 767 528 340 175 174
72
BtgI C'CryG_G
Cuts at: 0 1808 3656 4571 4838 5832
Size: 1808 1848 915 267 994
Fragments arranged by size:
1848 1808 994 915 267
BtsI GCAGTG_nn'
Cuts at: 0 78 1485 3764 5832
Size: 78 1407 2279 2068
Fragments arranged by size:
2279 2068 1407 78
ClaI AT'CG_AT
Cuts at: 0 3374 5832
Size: 3374 2458
DraI TTT'AAA
Cuts at: 0 848 4988 5832
Size: 848 4140 844
Fragments arranged by size:
4140 848 844
DraIII CAC_nnn'GTG
Cuts at: 0 2304 4925 5679 5832
Size: 2304 2621 754 153
Fragments arranged by size:
2621 2304 754 153
DrdI GACnn_nn'nnGTC
Cuts at: 0 4494 5832
Size: 4494 1338
EaeI y'GGCC_r
Cuts at: 0 884 2908 3503 4835 5832
Size: 884 2024 595 1332 997
Fragments arranged by size:
2024 1332 997 884 595
EarI CTCTTCn'nnn_
Cuts at: 0 827 2843 2861 3242 4754 5400 5749 5832
Size: 827 2016 18 381 1512 646 349 83
Fragments arranged by size:
2016 1512 827 646 381 349 83 18
EcoICRI GAG'CTC
Cuts at: 0 3168 5832
Size: 3168 2664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 581 1333 1925 2180 2575 2996 3106
Size: 166 415 752 592 255 395 421 110
Cuts at: 3106 3454 4792 5204 5300 5832
Size: 348 1338 412 96 532
Fragments arranged by size:
1338 752 592 532 421 415 412 395
348 255 166 110 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 1070 5074 5832
Size: 184 886 4004 758
Fragments arranged by size:
4004 886 758 184
EcoO109I rG'GnC_Cy
Cuts at: 0 1948 2398 4522 5832
Size: 1948 450 2124 1310
Fragments arranged by size:
2124 1948 1310 450
EcoRI G'AATT_C
Cuts at: 0 5527 5832
Size: 5527 305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 596 2074 4733 5832
Size: 596 1478 2659 1099
Fragments arranged by size:
2659 1478 1099 596
FspI TGC'GCA
Cuts at: 0 3131 5832
Size: 3131 2701
FspAI rTGC'GCAy
Cuts at: 0 3131 5832
Size: 3131 2701
HaeII r_GCGC'y
Cuts at: 0 578 3445 4005 5832
Size: 578 2867 560 1827
Fragments arranged by size:
2867 1827 578 560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1754 1786 2089 2121 2535 2567 2603 2635
Size: 1754 32 303 32 414 32 36 32
Cuts at: 2635 2659 2691 2777 2809 2922 2954 3055 3087
Size: 24 32 86 32 113 32 101 32
Cuts at: 3087 3117 3149 3859 3891 4212 4244 5832
Size: 30 32 710 32 321 32 1588
Fragments arranged by size:
1754 1588 710 414 321 303 113 101
86 36 32 32 32 32 32 32
32 32 32 32 32 30 24
HincII GTy'rAC
Cuts at: 0 357 3147 4278 5832
Size: 357 2790 1131 1554
Fragments arranged by size:
2790 1554 1131 357
HindIII A'AGCT_T
Cuts at: 0 2201 5727 5832
Size: 2201 3526 105
Fragments arranged by size:
3526 2201 105
MfeI C'AATT_G
Cuts at: 0 1205 2465 3214 5832
Size: 1205 1260 749 2618
Fragments arranged by size:
2618 1260 1205 749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 1589 1755 3310 3969 4166 5832
Size: 1058 531 166 1555 659 197 1666
Fragments arranged by size:
1666 1555 1058 659 531 197 166
MscI TGG'CCA
Cuts at: 0 886 2910 3505 5832
Size: 886 2024 595 2327
Fragments arranged by size:
2327 2024 886 595
MslI CAynn'nnrTG
Cuts at: 0 95 381 1504 1996 2150 2885 3078 3376
Size: 95 286 1123 492 154 735 193 298
Cuts at: 3376 3416 3661 4075 4576 4826 5374 5832
Size: 40 245 414 501 250 548 458
Fragments arranged by size:
1123 735 548 501 492 458 414 298
286 250 245 193 154 95 40
MspA1I CmG'CkG
Cuts at: 0 123 4840 5618 5643 5832
Size: 123 4717 778 25 189
Fragments arranged by size:
4717 778 189 123 25
NcoI C'CATG_G
Cuts at: 0 1808 3656 4571 5832
Size: 1808 1848 915 1261
Fragments arranged by size:
1848 1808 1261 915
NdeI CA'TA_TG
Cuts at: 0 451 5832
Size: 451 5381
NsiI A_TGCA'T
Cuts at: 0 451 485 2495 4022 5832
Size: 451 34 2010 1527 1810
Fragments arranged by size:
2010 1810 1527 451 34
NspI r_CATG'y
Cuts at: 0 483 639 3496 4384 5652 5832
Size: 483 156 2857 888 1268 180
Fragments arranged by size:
2857 1268 888 483 180 156
PasI CC'CwG_GG
Cuts at: 0 3903 5832
Size: 3903 1929
PciI A'CATG_T
Cuts at: 0 4380 5832
Size: 4380 1452
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 1597 4677 5832
Size: 330 1267 3080 1155
Fragments arranged by size:
3080 1267 1155 330
PfoI T'CCnGG_A
Cuts at: 0 1533 1941 5832
Size: 1533 408 3891
Fragments arranged by size:
3891 1533 408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3219 3251 5832
Size: 3219 32 2581
Fragments arranged by size:
3219 2581 32
PpuMI rG'GwC_Cy
Cuts at: 0 1948 4522 5832
Size: 1948 2574 1310
Fragments arranged by size:
2574 1948 1310
PsiI TTA'TAA
Cuts at: 0 341 2009 4300 5832
Size: 341 1668 2291 1532
Fragments arranged by size:
2291 1668 1532 341
PstI C_TGCA'G
Cuts at: 0 128 3759 3810 5492 5832
Size: 128 3631 51 1682 340
Fragments arranged by size:
3631 1682 340 128 51
PvuII CAG'CTG
Cuts at: 0 123 5643 5832
Size: 123 5520 189
Fragments arranged by size:
5520 189 123
SacI G_AGCT'C
Cuts at: 0 3170 5832
Size: 3170 2662
SacII CC_GC'GG
Cuts at: 0 4841 5832
Size: 4841 991
SapI GCTCTTCn'nnn_
Cuts at: 0 2861 3242 4754 5749 5832
Size: 2861 381 1512 995 83
Fragments arranged by size:
2861 1512 995 381 83
ScaI AGT'ACT
Cuts at: 0 1587 1608 5321 5832
Size: 1587 21 3713 511
Fragments arranged by size:
3713 1587 511 21
SexAI A'CCwGG_T
Cuts at: 0 1487 5832
Size: 1487 4345
SfcI C'TryA_G
Cuts at: 0 124 423 2418 2699 3242 3755 3806 5488
Size: 124 299 1995 281 543 513 51 1682
Cuts at: 5488 5705 5832
Size: 217 127
Fragments arranged by size:
1995 1682 543 513 299 281 217 127
124 51
SmaI CCC'GGG
Cuts at: 0 5191 5832
Size: 5191 641
SmlI C'TyrA_G
Cuts at: 0 2317 2622 5832
Size: 2317 305 3210
Fragments arranged by size:
3210 2317 305
SphI G_CATG'C
Cuts at: 0 639 3496 5652 5832
Size: 639 2857 2156 180
Fragments arranged by size:
2857 2156 639 180
SrfI GCCC'GGGC
Cuts at: 0 5191 5832
Size: 5191 641
SspI AAT'ATT
Cuts at: 0 2125 5257 5832
Size: 2125 3132 575
Fragments arranged by size:
3132 2125 575
StuI AGG'CCT
Cuts at: 0 421 2091 2959 5832
Size: 421 1670 868 2873
Fragments arranged by size:
2873 1670 868 421
StyI C'CwwG_G
Cuts at: 0 1632 1808 3593 3656 4571 4683 5075 5627
Size: 1632 176 1785 63 915 112 392 552
Cuts at: 5627 5832
Size: 205
Fragments arranged by size:
1785 1632 915 552 392 205 176 112
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 621 659 1017 2529 5832
Size: 621 38 358 1512 3303
Fragments arranged by size:
3303 1512 621 358 38
TatI w'GTAC_w
Cuts at: 0 1585 1606 1723 1851 2636 3980 5319 5832
Size: 1585 21 117 128 785 1344 1339 513
Fragments arranged by size:
1585 1344 1339 785 513 128 117 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 281 313 1475 1507 2454 2486
Size: 242 32 7 32 1162 32 947 32
Cuts at: 2486 5047 5079 5214 5246 5832
Size: 2561 32 135 32 586
Fragments arranged by size:
2561 1162 947 586 242 135 32 32
32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 1560 5832
Size: 1560 4272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1399 1467 4182 4268 4681 5832
Size: 1399 68 2715 86 413 1151
Fragments arranged by size:
2715 1399 1151 413 86 68
XmaI C'CCGG_G
Cuts at: 0 5189 5832
Size: 5189 643
XmnI GAAnn'nnTTC
Cuts at: 0 59 576 2349 2837 5832
Size: 59 517 1773 488 2995
Fragments arranged by size:
2995 1773 517 488 59
ZraI GAC'GTC
Cuts at: 0 2938 5832
Size: 2938 2894
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtsI ClaI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI
PstI PvuII SacI SacII SapI ScaI SexAI SfcI
SmaI SmlI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AloI ApaI AscI AsiSI AvrII BaeI
BbeI BciVI BclI BglI BmgBI BmtI BsaAI BsaBI
BsiEI BsiWI BsmBI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI EagI EciI EcoRV FseI HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SwaI XbaI XhoI