Restriction map for
pF1KB9390
(Linear) (Six-base) MAPSORT of: KIBB9390.seq Check: 9729 from: 1 to: 735
KIBB9390.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 377 607 735
Size: 155 222 230 128
Fragments arranged by size:
230 222 155 128
AlwNI CAG_nnn'CTG
Cuts at: 0 228 735
Size: 228 507
ApoI r'AATT_y
Cuts at: 0 317 735
Size: 317 418
AvaI C'yCGr_G
Cuts at: 0 602 735
Size: 602 133
BanI G'GyrC_C
Cuts at: 0 85 735
Size: 85 650
BanII G_rGCy'C
Cuts at: 0 294 446 735
Size: 294 152 289
Fragments arranged by size:
294 289 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 735
Size: 551 184
BbvCI CC'TCA_GC
Cuts at: 0 71 121 735
Size: 71 50 614
Fragments arranged by size:
614 71 50
BlpI GC'TnA_GC
Cuts at: 0 440 735
Size: 440 295
Bme1580I G_kGCm'C
Cuts at: 0 88 198 735
Size: 88 110 537
Fragments arranged by size:
537 110 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 549 735
Size: 549 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 250 532 735
Size: 40 210 282 203
Fragments arranged by size:
282 210 203 40
Bpu10I CC'TnA_GC
Cuts at: 0 71 121 518 735
Size: 71 50 397 217
Fragments arranged by size:
397 217 71 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 735
Size: 703 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 735
Size: 121 614
BsaHI Gr'CG_yC
Cuts at: 0 65 735
Size: 65 670
BsaWI w'CCGG_w
Cuts at: 0 41 735
Size: 41 694
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 445 735
Size: 88 357 290
Fragments arranged by size:
357 290 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 735
Size: 446 289
BsiEI CG_ry'CG
Cuts at: 0 369 735
Size: 369 366
BsiHKAI G_wGCw'C
Cuts at: 0 446 735
Size: 446 289
Bsp1286I G_dGCh'C
Cuts at: 0 88 198 294 446 735
Size: 88 110 96 152 289
Fragments arranged by size:
289 152 110 96 88
BspEI T'CCGG_A
Cuts at: 0 41 735
Size: 41 694
BsrBI CCG'CTC
Cuts at: 0 435 735
Size: 435 300
BsrDI GCAATG_nn'
Cuts at: 0 350 735
Size: 350 385
BssHII G'CGCG_C
Cuts at: 0 78 735
Size: 78 657
BssSI C'ACGA_G
Cuts at: 0 109 735
Size: 109 626
BstYI r'GATC_y
Cuts at: 0 38 326 735
Size: 38 288 409
Fragments arranged by size:
409 288 38
EarI CTCTTCn'nnn_
Cuts at: 0 176 374 554 735
Size: 176 198 180 181
Fragments arranged by size:
198 181 180 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 735
Size: 76 659
EcoICRI GAG'CTC
Cuts at: 0 444 735
Size: 444 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 155 250 377 532 607 735
Size: 40 115 95 127 155 75 128
Fragments arranged by size:
155 128 127 115 95 75 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 727 735
Size: 727 8
EcoO109I rG'GnC_Cy
Cuts at: 0 570 735
Size: 570 165
FspI TGC'GCA
Cuts at: 0 31 735
Size: 31 704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 354 602 735
Size: 167 187 248 133
Fragments arranged by size:
248 187 167 133
MspA1I CmG'CkG
Cuts at: 0 70 133 171 735
Size: 70 63 38 564
Fragments arranged by size:
564 70 63 38
PpuMI rG'GwC_Cy
Cuts at: 0 570 735
Size: 570 165
PstI C_TGCA'G
Cuts at: 0 53 95 735
Size: 53 42 640
Fragments arranged by size:
640 53 42
PvuII CAG'CTG
Cuts at: 0 171 735
Size: 171 564
SacI G_AGCT'C
Cuts at: 0 446 735
Size: 446 289
SbfI CC_TGCA'GG
Cuts at: 0 53 735
Size: 53 682
ScaI AGT'ACT
Cuts at: 0 532 735
Size: 532 203
SfcI C'TryA_G
Cuts at: 0 49 91 735
Size: 49 42 644
Fragments arranged by size:
644 49 42
SmlI C'TyrA_G
Cuts at: 0 682 735
Size: 682 53
TatI w'GTAC_w
Cuts at: 0 530 735
Size: 530 205
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanI BanII BbsI BbvCI
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI
BsrDI BssHII BssSI BstYI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI MmeI MspA1I PpuMI PstI PvuII
SacI SbfI ScaI SfcI SmlI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BsaAI
BsaBI BsaXI BseYI BsiWI BsmI BsmBI BspHI BspMI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoRI EcoRV FalI FseI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI