Restriction map for
pF1KB9394
(Linear) (Six-base) MAPSORT of: KIBB9394.seq Check: 6542 from: 1 to: 1164
KIBB9394.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 189 557 1164
Size: 189 368 607
Fragments arranged by size:
607 368 189
Acc65I G'GTAC_C
Cuts at: 0 610 1164
Size: 610 554
AclI AA'CG_TT
Cuts at: 0 644 1164
Size: 644 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1164
Size: 329 835
AlwNI CAG_nnn'CTG
Cuts at: 0 576 1164
Size: 576 588
ApaI G_GGCC'C
Cuts at: 0 370 1164
Size: 370 794
AvaI C'yCGr_G
Cuts at: 0 1138 1164
Size: 1138 26
BanI G'GyrC_C
Cuts at: 0 102 610 1164
Size: 102 508 554
Fragments arranged by size:
554 508 102
BanII G_rGCy'C
Cuts at: 0 370 1164
Size: 370 794
BbeI G_GCGC'C
Cuts at: 0 106 1164
Size: 106 1058
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 1164
Size: 317 847
BbvCI CC'TCA_GC
Cuts at: 0 1030 1164
Size: 1030 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 221 1164
Size: 187 34 943
Fragments arranged by size:
943 187 34
Bme1580I G_kGCm'C
Cuts at: 0 370 1164
Size: 370 794
BmrI ACTGGGnnnn_n'
Cuts at: 0 526 1164
Size: 526 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1164
Size: 547 617
Bpu10I CC'TnA_GC
Cuts at: 0 185 1030 1148 1164
Size: 185 845 118 16
Fragments arranged by size:
845 185 118 16
BsaBI GATnn'nnATC
Cuts at: 0 499 704 1164
Size: 499 205 460
Fragments arranged by size:
499 460 205
BsaHI Gr'CG_yC
Cuts at: 0 103 1103 1164
Size: 103 1000 61
Fragments arranged by size:
1000 103 61
BsaWI w'CCGG_w
Cuts at: 0 509 714 1164
Size: 509 205 450
Fragments arranged by size:
509 450 205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1010 1164
Size: 1010 154
BsiHKAI G_wGCw'C
Cuts at: 0 186 527 1164
Size: 186 341 637
Fragments arranged by size:
637 341 186
BsmI GAATG_Cn'
Cuts at: 0 149 930 1164
Size: 149 781 234
Fragments arranged by size:
781 234 149
Bsp1286I G_dGCh'C
Cuts at: 0 186 370 527 1164
Size: 186 184 157 637
Fragments arranged by size:
637 186 184 157
BspEI T'CCGG_A
Cuts at: 0 509 1164
Size: 509 655
BspHI T'CATG_A
Cuts at: 0 131 411 696 1164
Size: 131 280 285 468
Fragments arranged by size:
468 285 280 131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 346 557 1164
Size: 189 157 211 607
Fragments arranged by size:
607 211 189 157
BsrBI CCG'CTC
Cuts at: 0 38 1164
Size: 38 1126
BsrDI GCAATG_nn'
Cuts at: 0 668 1164
Size: 668 496
BsrFI r'CCGG_y
Cuts at: 0 944 1164
Size: 944 220
BssHII G'CGCG_C
Cuts at: 0 3 1164
Size: 3 1161
BssSI C'ACGA_G
Cuts at: 0 520 1164
Size: 520 644
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1012 1164
Size: 1012 152
BtsI GCAGTG_nn'
Cuts at: 0 240 1164
Size: 240 924
EaeI y'GGCC_r
Cuts at: 0 946 1164
Size: 946 218
EarI CTCTTCn'nnn_
Cuts at: 0 61 133 424 1164
Size: 61 72 291 740
Fragments arranged by size:
740 291 72 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 833 1164
Size: 833 331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 547 1164
Size: 329 218 617
Fragments arranged by size:
617 329 218
EcoO109I rG'GnC_Cy
Cuts at: 0 366 1164
Size: 366 798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 371 1164
Size: 371 793
HaeII r_GCGC'y
Cuts at: 0 106 570 576 1164
Size: 106 464 6 588
Fragments arranged by size:
588 464 106 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 986 1018 1164
Size: 986 32 146
Fragments arranged by size:
986 146 32
HincII GTy'rAC
Cuts at: 0 1128 1164
Size: 1128 36
KasI G'GCGC_C
Cuts at: 0 102 1164
Size: 102 1062
KpnI G_GTAC'C
Cuts at: 0 614 1164
Size: 614 550
MslI CAynn'nnrTG
Cuts at: 0 382 1164
Size: 382 782
NaeI GCC'GGC
Cuts at: 0 946 1164
Size: 946 218
NarI GG'CG_CC
Cuts at: 0 103 1164
Size: 103 1061
NgoMIV G'CCGG_C
Cuts at: 0 944 1164
Size: 944 220
NspI r_CATG'y
Cuts at: 0 477 1164
Size: 477 687
PfoI T'CCnGG_A
Cuts at: 0 158 1164
Size: 158 1006
PspOMI G'GGCC_C
Cuts at: 0 366 1164
Size: 366 798
PstI C_TGCA'G
Cuts at: 0 581 1164
Size: 581 583
SapI GCTCTTCn'nnn_
Cuts at: 0 61 1164
Size: 61 1103
SfcI C'TryA_G
Cuts at: 0 577 1164
Size: 577 587
SfoI GGC'GCC
Cuts at: 0 104 1164
Size: 104 1060
SphI G_CATG'C
Cuts at: 0 477 1164
Size: 477 687
StyI C'CwwG_G
Cuts at: 0 144 1164
Size: 144 1020
TatI w'GTAC_w
Cuts at: 0 86 918 968 1164
Size: 86 832 50 196
Fragments arranged by size:
832 196 86 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 1164
Size: 219 32 913
Fragments arranged by size:
913 219 32
XmnI GAAnn'nnTTC
Cuts at: 0 845 1164
Size: 845 319
Enzymes that do cut:
AarI Acc65I AclI AcuI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI Bme1580I BmrI BpmI
Bpu10I BsaBI BsaHI BsaWI BseRI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstXI BtsI EaeI EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I HincII KasI KpnI MslI NaeI NarI
NgoMIV NspI PfoI PspOMI PstI SapI SfcI SfoI
SphI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BsrGI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI MfeI MluI MmeI MscI
MspA1I NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI ZraI