Restriction map for
pF1KB9403
(Linear) (Six-base) MAPSORT of: KIBB9403.seq Check: 9505 from: 1 to: 3816
KIBB9403.orf
With 182 enzymes: SgfI *
March 31, 2008 15:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3777 3816
Size: 3777 39
AatII G_ACGT'C
Cuts at: 0 2078 3816
Size: 2078 1738
Acc65I G'GTAC_C
Cuts at: 0 1232 3032 3608 3816
Size: 1232 1800 576 208
Fragments arranged by size:
1800 1232 576 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 886 1534 1996 2062 2356 2383 2469
Size: 768 118 648 462 66 294 27 86
Cuts at: 2469 2698 3816
Size: 229 1118
Fragments arranged by size:
1118 768 648 462 294 229 118 86
66 27
AfeI AGC'GCT
Cuts at: 0 719 1929 3553 3816
Size: 719 1210 1624 263
Fragments arranged by size:
1624 1210 719 263
AleI CACnn'nnGTG
Cuts at: 0 2857 3816
Size: 2857 959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 646 1684 3816
Size: 646 1038 2132
Fragments arranged by size:
2132 1038 646
AlwNI CAG_nnn'CTG
Cuts at: 0 849 2373 3042 3393 3808 3816
Size: 849 1524 669 351 415 8
Fragments arranged by size:
1524 849 669 415 351 8
ApaI G_GGCC'C
Cuts at: 0 1323 3022 3816
Size: 1323 1699 794
Fragments arranged by size:
1699 1323 794
ApaLI G'TGCA_C
Cuts at: 0 700 3816
Size: 700 3116
ApoI r'AATT_y
Cuts at: 0 286 2489 3816
Size: 286 2203 1327
Fragments arranged by size:
2203 1327 286
AvaI C'yCGr_G
Cuts at: 0 141 616 1440 3816
Size: 141 475 824 2376
Fragments arranged by size:
2376 824 475 141
AvrII C'CTAG_G
Cuts at: 0 3006 3816
Size: 3006 810
BanI G'GyrC_C
Cuts at: 0 202 1232 1552 1677 2529 3032 3608 3816
Size: 202 1030 320 125 852 503 576 208
Fragments arranged by size:
1030 852 576 503 320 208 202 125
BanII G_rGCy'C
Cuts at: 0 134 748 1323 1450 2142 2291 3022 3059
Size: 134 614 575 127 692 149 731 37
Cuts at: 3059 3816
Size: 757
Fragments arranged by size:
757 731 692 614 575 149 134 127
37
BbeI G_GCGC'C
Cuts at: 0 206 3816
Size: 206 3610
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 1336 1734 2132 3816
Size: 66 1270 398 398 1684
Fragments arranged by size:
1684 1270 398 398 66
BbvCI CC'TCA_GC
Cuts at: 0 2692 2980 3328 3816
Size: 2692 288 348 488
Fragments arranged by size:
2692 488 348 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 1093 1127 3621 3655 3816
Size: 93 34 966 34 2494 34 161
Fragments arranged by size:
2494 966 161 93 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 65 1145 3766 3816
Size: 65 1080 2621 50
Fragments arranged by size:
2621 1080 65 50
BglII A'GATC_T
Cuts at: 0 2430 3348 3816
Size: 2430 918 468
Fragments arranged by size:
2430 918 468
BlpI GC'TnA_GC
Cuts at: 0 2285 3816
Size: 2285 1531
Bme1580I G_kGCm'C
Cuts at: 0 704 1323 1682 1848 3022 3816
Size: 704 619 359 166 1174 794
Fragments arranged by size:
1174 794 704 619 359 166
BmgBI CAC'GTC
Cuts at: 0 3071 3816
Size: 3071 745
BmrI ACTGGGnnnn_n'
Cuts at: 0 1320 1928 2591 3295 3816
Size: 1320 608 663 704 521
Fragments arranged by size:
1320 704 663 608 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2305 3816
Size: 2305 1511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 415 1075 1954 2008 2098 2197 2277
Size: 257 158 660 879 54 90 99 80
Cuts at: 2277 2650 3064 3253 3365 3502 3816
Size: 373 414 189 112 137 314
Fragments arranged by size:
879 660 414 373 314 257 189 158
137 112 99 90 80 54
Bpu10I CC'TnA_GC
Cuts at: 0 35 2692 2966 2980 3328 3548 3816
Size: 35 2657 274 14 348 220 268
Fragments arranged by size:
2657 348 274 268 220 35 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2011 2854 3473 3610 3816
Size: 2011 843 619 137 206
Fragments arranged by size:
2011 843 619 206 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 452 1091 2204 2891 3130 3816
Size: 452 639 1113 687 239 686
Fragments arranged by size:
1113 687 686 639 452 239
BsaAI yAC'GTr
Cuts at: 0 642 2664 3558 3816
Size: 642 2022 894 258
Fragments arranged by size:
2022 894 642 258
BsaHI Gr'CG_yC
Cuts at: 0 203 2075 3816
Size: 203 1872 1741
Fragments arranged by size:
1872 1741 203
BsaWI w'CCGG_w
Cuts at: 0 1070 1794 2514 3116 3816
Size: 1070 724 720 602 700
Fragments arranged by size:
1070 724 720 700 602
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1873 1903 2932 2962 3084 3114 3169 3199
Size: 1873 30 1029 30 122 30 55 30
Cuts at: 3199 3816
Size: 617
Fragments arranged by size:
1873 1029 617 122 55 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 211 1120 1440 1818 1821 3238 3445
Size: 196 15 909 320 378 3 1417 207
Cuts at: 3445 3607 3816
Size: 162 209
Fragments arranged by size:
1417 909 378 320 209 207 196 162
15 3
BseYI C'CCAG_C
Cuts at: 0 176 376 634 1824 2219 2648 2687 2907
Size: 176 200 258 1190 395 429 39 220
Cuts at: 2907 2996 3583 3773 3816
Size: 89 587 190 43
Fragments arranged by size:
1190 587 429 395 258 220 200 190
176 89 43 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 496 824 3239 3472 3816
Size: 84 412 328 2415 233 344
Fragments arranged by size:
2415 412 344 328 233 84
BsiEI CG_ry'CG
Cuts at: 0 17 859 2950 3337 3816
Size: 17 842 2091 387 479
Fragments arranged by size:
2091 842 479 387 17
BsiHKAI G_wGCw'C
Cuts at: 0 704 1310 1429 2291 3816
Size: 704 606 119 862 1525
Fragments arranged by size:
1525 862 704 606 119
BsmI GAATG_Cn'
Cuts at: 0 241 1190 3816
Size: 241 949 2626
Fragments arranged by size:
2626 949 241
Bsp1286I G_dGCh'C
Cuts at: 0 134 704 748 1310 1323 1429 1450 1682
Size: 134 570 44 562 13 106 21 232
Cuts at: 1682 1848 2142 2291 3022 3059 3816
Size: 166 294 149 731 37 757
Fragments arranged by size:
757 731 570 562 294 232 166 149
134 106 44 37 21 13
BspEI T'CCGG_A
Cuts at: 0 2514 3116 3816
Size: 2514 602 700
Fragments arranged by size:
2514 700 602
BspHI T'CATG_A
Cuts at: 0 930 3816
Size: 930 2886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 1478 3020 3392 3777 3816
Size: 173 1305 1542 372 385 39
Fragments arranged by size:
1542 1305 385 372 173 39
BsrBI CCG'CTC
Cuts at: 0 158 891 1685 3339 3816
Size: 158 733 794 1654 477
Fragments arranged by size:
1654 794 733 477 158
BsrDI GCAATG_nn'
Cuts at: 0 3095 3816
Size: 3095 721
BsrFI r'CCGG_y
Cuts at: 0 3332 3816
Size: 3332 484
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2253 2373 3459 3816
Size: 2253 120 1086 357
Fragments arranged by size:
2253 1086 357 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 448 1378 2310 3816
Size: 448 930 932 1506
Fragments arranged by size:
1506 932 930 448
BstYI r'GATC_y
Cuts at: 0 1998 2430 3348 3567 3626 3816
Size: 1998 432 918 219 59 190
Fragments arranged by size:
1998 918 432 219 190 59
Bsu36I CC'TnA_GG
Cuts at: 0 662 3675 3816
Size: 662 3013 141
Fragments arranged by size:
3013 662 141
BtgI C'CryG_G
Cuts at: 0 171 371 491 1901 3816
Size: 171 200 120 1410 1915
Fragments arranged by size:
1915 1410 200 171 120
BtsI GCAGTG_nn'
Cuts at: 0 2453 2920 3809 3816
Size: 2453 467 889 7
Fragments arranged by size:
2453 889 467 7
DraIII CAC_nnn'GTG
Cuts at: 0 1559 3816
Size: 1559 2257
DrdI GACnn_nn'nnGTC
Cuts at: 0 2074 3816
Size: 2074 1742
EaeI y'GGCC_r
Cuts at: 0 66 939 981 1380 1756 3334 3816
Size: 66 873 42 399 376 1578 482
Fragments arranged by size:
1578 873 482 399 376 66 42
EagI C'GGCC_G
Cuts at: 0 3334 3816
Size: 3334 482
EarI CTCTTCn'nnn_
Cuts at: 0 296 448 862 1421 1523 2385 2647 3569
Size: 296 152 414 559 102 862 262 922
Cuts at: 3569 3816
Size: 247
Fragments arranged by size:
922 862 559 414 296 262 247 152
102
EcoICRI GAG'CTC
Cuts at: 0 2289 3816
Size: 2289 1527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 415 768 886 1075 1534 1954 1996
Size: 257 158 353 118 189 459 420 42
Cuts at: 1996 2008 2062 2098 2197 2277 2356 2383 2469
Size: 12 54 36 99 80 79 27 86
Cuts at: 2469 2650 2698 3064 3253 3365 3502 3816
Size: 181 48 366 189 112 137 314
Fragments arranged by size:
459 420 366 353 314 257 189 189
181 158 137 118 112 99 86 80
79 54 48 42 36 27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1724 2026 3816
Size: 1724 302 1790
Fragments arranged by size:
1790 1724 302
EcoO109I rG'GnC_Cy
Cuts at: 0 773 949 1047 1319 1375 1729 2019 2109
Size: 773 176 98 272 56 354 290 90
Cuts at: 2109 2265 2768 2865 3019 3258 3816
Size: 156 503 97 154 239 558
Fragments arranged by size:
773 558 503 354 290 272 239 176
156 154 98 97 90 56
EcoRI G'AATT_C
Cuts at: 0 286 2489 3816
Size: 286 2203 1327
Fragments arranged by size:
2203 1327 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3168 3189 3816
Size: 3168 21 627
Fragments arranged by size:
3168 627 21
FspI TGC'GCA
Cuts at: 0 1493 3816
Size: 1493 2323
HaeII r_GCGC'y
Cuts at: 0 206 721 1536 1587 1618 1931 3183 3555
Size: 206 515 815 51 31 313 1252 372
Cuts at: 3555 3816
Size: 261
Fragments arranged by size:
1252 815 515 372 313 261 206 51
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 401 433 707 739 954 986
Size: 28 32 341 32 274 32 215 32
Cuts at: 986 1350 1382 1873 1905 2932 2964 3340 3372
Size: 364 32 491 32 1027 32 376 32
Cuts at: 3372 3523 3555 3816
Size: 151 32 261
Fragments arranged by size:
1027 491 376 364 341 274 261 215
151 32 32 32 32 32 32 32
32 32 28
HincII GTy'rAC
Cuts at: 0 138 999 3816
Size: 138 861 2817
Fragments arranged by size:
2817 861 138
KasI G'GCGC_C
Cuts at: 0 202 3816
Size: 202 3614
KpnI G_GTAC'C
Cuts at: 0 1236 3036 3612 3816
Size: 1236 1800 576 204
Fragments arranged by size:
1800 1236 576 204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2321 3455 3816
Size: 2321 1134 361
Fragments arranged by size:
2321 1134 361
MscI TGG'CCA
Cuts at: 0 983 1382 3816
Size: 983 399 2434
Fragments arranged by size:
2434 983 399
MslI CAynn'nnrTG
Cuts at: 0 709 935 1547 1693 2308 2857 3816
Size: 709 226 612 146 615 549 959
Fragments arranged by size:
959 709 615 612 549 226 146
MspA1I CmG'CkG
Cuts at: 0 98 173 742 1239 1974 2361 2652 2691
Size: 98 75 569 497 735 387 291 39
Cuts at: 2691 2984 3231 3717 3816
Size: 293 247 486 99
Fragments arranged by size:
735 569 497 486 387 293 291 247
99 98 75 39
NaeI GCC'GGC
Cuts at: 0 3334 3816
Size: 3334 482
NarI GG'CG_CC
Cuts at: 0 203 3816
Size: 203 3613
NcoI C'CATG_G
Cuts at: 0 1901 3816
Size: 1901 1915
NdeI CA'TA_TG
Cuts at: 0 317 1200 3816
Size: 317 883 2616
Fragments arranged by size:
2616 883 317
NgoMIV G'CCGG_C
Cuts at: 0 3332 3816
Size: 3332 484
NspI r_CATG'y
Cuts at: 0 1671 3657 3816
Size: 1671 1986 159
Fragments arranged by size:
1986 1671 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 2121 2226 3816
Size: 2121 105 1590
Fragments arranged by size:
2121 1590 105
PfoI T'CCnGG_A
Cuts at: 0 2114 2405 3816
Size: 2114 291 1411
Fragments arranged by size:
2114 1411 291
PpuMI rG'GwC_Cy
Cuts at: 0 773 949 1047 1375 1729 2019 2109 2265
Size: 773 176 98 328 354 290 90 156
Cuts at: 2265 3816
Size: 1551
Fragments arranged by size:
1551 773 354 328 290 176 156 98
90
PspOMI G'GGCC_C
Cuts at: 0 1319 3018 3816
Size: 1319 1699 798
Fragments arranged by size:
1699 1319 798
PstI C_TGCA'G
Cuts at: 0 2261 2378 2460 3389 3602 3722 3816
Size: 2261 117 82 929 213 120 94
Fragments arranged by size:
2261 929 213 120 117 94 82
PvuI CG_AT'CG
Cuts at: 0 859 3816
Size: 859 2957
PvuII CAG'CTG
Cuts at: 0 1974 2361 2652 2691 3231 3717 3816
Size: 1974 387 291 39 540 486 99
Fragments arranged by size:
1974 540 486 387 291 99 39
SacI G_AGCT'C
Cuts at: 0 2291 3816
Size: 2291 1525
SacII CC_GC'GG
Cuts at: 0 174 3816
Size: 174 3642
SanDI GG'GwC_CC
Cuts at: 0 949 1047 3816
Size: 949 98 2769
Fragments arranged by size:
2769 949 98
SapI GCTCTTCn'nnn_
Cuts at: 0 296 1523 2385 3569 3816
Size: 296 1227 862 1184 247
Fragments arranged by size:
1227 1184 862 296 247
SbfI CC_TGCA'GG
Cuts at: 0 2378 3816
Size: 2378 1438
SfcI C'TryA_G
Cuts at: 0 2257 2374 2456 2787 3385 3598 3664 3718
Size: 2257 117 82 331 598 213 66 54
Cuts at: 3718 3816
Size: 98
Fragments arranged by size:
2257 598 331 213 117 98 82 66
54
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1145 3766 3816
Size: 1145 2621 50
Fragments arranged by size:
2621 1145 50
SfoI GGC'GCC
Cuts at: 0 204 3816
Size: 204 3612
SmlI C'TyrA_G
Cuts at: 0 2026 2869 3488 3589 3816
Size: 2026 843 619 101 227
Fragments arranged by size:
2026 843 619 227 101
SphI G_CATG'C
Cuts at: 0 1671 3816
Size: 1671 2145
SspI AAT'ATT
Cuts at: 0 685 3816
Size: 685 3131
StuI AGG'CCT
Cuts at: 0 2462 3769 3816
Size: 2462 1307 47
Fragments arranged by size:
2462 1307 47
StyI C'CwwG_G
Cuts at: 0 1349 1626 1735 1837 1901 3006 3173 3291
Size: 1349 277 109 102 64 1105 167 118
Cuts at: 3291 3816
Size: 525
Fragments arranged by size:
1349 1105 525 277 167 118 109 102
64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 811 1514 1898 2696 3597 3816
Size: 811 703 384 798 901 219
Fragments arranged by size:
901 811 798 703 384 219
TatI w'GTAC_w
Cuts at: 0 3185 3816
Size: 3185 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 907 939 3084 3116 3816
Size: 907 32 2145 32 700
Fragments arranged by size:
2145 907 700 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 994 2107 2269 3816
Size: 994 1113 162 1547
Fragments arranged by size:
1547 1113 994 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 1899 3816
Size: 489 1410 1917
Fragments arranged by size:
1917 1410 489
XmnI GAAnn'nnTTC
Cuts at: 0 290 3816
Size: 290 3526
ZraI GAC'GTC
Cuts at: 0 2076 3816
Size: 2076 1740
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PflMI PfoI PpuMI PspOMI PstI PvuI PvuII SacI
SacII SanDI SapI SbfI SfcI SfiI SfoI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AhdI AloI AscI
AseI AsiSI BaeI BamHI BciVI BclI BfrBI BmtI
BsaBI BsiWI BsmBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI EciI EcoRV FseI
FspAI HindIII HpaI MfeI MluI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI RsrII SalI ScaI SexAI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI XhoI
XmaI