Restriction map for
pF1KB9405
(Linear) (Six-base) MAPSORT of: KIBB9405.seq Check: 5609 from: 1 to: 1512
KIBB9405.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1512
Size: 668 844
AhdI GACnn_n'nnGTC
Cuts at: 0 1458 1512
Size: 1458 54
AlwNI CAG_nnn'CTG
Cuts at: 0 340 930 1512
Size: 340 590 582
Fragments arranged by size:
590 582 340
ApaI G_GGCC'C
Cuts at: 0 85 1512
Size: 85 1427
ApaLI G'TGCA_C
Cuts at: 0 163 1512
Size: 163 1349
ApoI r'AATT_y
Cuts at: 0 734 1014 1512
Size: 734 280 498
Fragments arranged by size:
734 498 280
AvaI C'yCGr_G
Cuts at: 0 7 97 1102 1178 1512
Size: 7 90 1005 76 334
Fragments arranged by size:
1005 334 90 76 7
BamHI G'GATC_C
Cuts at: 0 3 1512
Size: 3 1509
BanI G'GyrC_C
Cuts at: 0 108 129 138 1165 1512
Size: 108 21 9 1027 347
Fragments arranged by size:
1027 347 108 21 9
BanII G_rGCy'C
Cuts at: 0 85 302 351 375 868 1512
Size: 85 217 49 24 493 644
Fragments arranged by size:
644 493 217 85 49 24
BbeI G_GCGC'C
Cuts at: 0 133 1512
Size: 133 1379
BbsI GAAGACnn'nnnn_
Cuts at: 0 479 1088 1512
Size: 479 609 424
Fragments arranged by size:
609 479 424
BciVI GTATCCnnnnn_n'
Cuts at: 0 1263 1512
Size: 1263 249
BglI GCCn_nnn'nGGC
Cuts at: 0 258 1512
Size: 258 1254
BlpI GC'TnA_GC
Cuts at: 0 470 1512
Size: 470 1042
Bme1580I G_kGCm'C
Cuts at: 0 85 167 242 254 1512
Size: 85 82 75 12 1258
Fragments arranged by size:
1258 85 82 75 12
BmgBI CAC'GTC
Cuts at: 0 168 1512
Size: 168 1344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 368 455 815 1084 1512
Size: 214 154 87 360 269 428
Fragments arranged by size:
428 360 269 214 154 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 1512
Size: 178 1334
BsaHI Gr'CG_yC
Cuts at: 0 130 878 1512
Size: 130 748 634
Fragments arranged by size:
748 634 130
BsaWI w'CCGG_w
Cuts at: 0 91 263 1512
Size: 91 172 1249
Fragments arranged by size:
1249 172 91
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1417 1512
Size: 1417 95
BseYI C'CCAG_C
Cuts at: 0 450 1003 1512
Size: 450 553 509
Fragments arranged by size:
553 509 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1512
Size: 679 833
BsiHKAI G_wGCw'C
Cuts at: 0 167 375 831 1512
Size: 167 208 456 681
Fragments arranged by size:
681 456 208 167
Bsp1286I G_dGCh'C
Cuts at: 0 85 167 242 254 302 351 375 831
Size: 85 82 75 12 48 49 24 456
Cuts at: 831 868 1512
Size: 37 644
Fragments arranged by size:
644 456 85 82 75 49 48 37
24 12
BspEI T'CCGG_A
Cuts at: 0 91 263 1512
Size: 91 172 1249
Fragments arranged by size:
1249 172 91
BspHI T'CATG_A
Cuts at: 0 215 1512
Size: 215 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 514 1512
Size: 514 998
BsrDI GCAATG_nn'
Cuts at: 0 992 1512
Size: 992 520
BsrFI r'CCGG_y
Cuts at: 0 30 105 1512
Size: 30 75 1407
Fragments arranged by size:
1407 75 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 340 915 930 972 987 1512
Size: 340 575 15 42 15 525
Fragments arranged by size:
575 525 340 42 15 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 527 1512
Size: 527 985
BstYI r'GATC_y
Cuts at: 0 3 1146 1512
Size: 3 1143 366
Fragments arranged by size:
1143 366 3
BtgI C'CryG_G
Cuts at: 0 113 171 1512
Size: 113 58 1341
Fragments arranged by size:
1341 113 58
BtsI GCAGTG_nn'
Cuts at: 0 338 1512
Size: 338 1174
ClaI AT'CG_AT
Cuts at: 0 578 1512
Size: 578 934
EaeI y'GGCC_r
Cuts at: 0 230 1512
Size: 230 1282
EarI CTCTTCn'nnn_
Cuts at: 0 208 698 1512
Size: 208 490 814
Fragments arranged by size:
814 490 208
EcoICRI GAG'CTC
Cuts at: 0 373 1512
Size: 373 1139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 368 455 668 815 1084 1512
Size: 214 154 87 213 147 269 428
Fragments arranged by size:
428 269 214 213 154 147 87
EcoO109I rG'GnC_Cy
Cuts at: 0 81 600 688 1512
Size: 81 519 88 824
Fragments arranged by size:
824 519 88 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 991 1512
Size: 991 521
HaeII r_GCGC'y
Cuts at: 0 133 202 1512
Size: 133 69 1310
Fragments arranged by size:
1310 133 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 804 836 1071 1103 1401 1433
Size: 289 32 483 32 235 32 298 32
Cuts at: 1433 1444 1476 1512
Size: 11 32 36
Fragments arranged by size:
483 298 289 235 36 32 32 32
32 32 11
HindIII A'AGCT_T
Cuts at: 0 1427 1494 1512
Size: 1427 67 18
Fragments arranged by size:
1427 67 18
KasI G'GCGC_C
Cuts at: 0 129 1512
Size: 129 1383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1512
Size: 923 589
MspA1I CmG'CkG
Cuts at: 0 115 173 1512
Size: 115 58 1339
Fragments arranged by size:
1339 115 58
NarI GG'CG_CC
Cuts at: 0 130 1512
Size: 130 1382
NspI r_CATG'y
Cuts at: 0 367 1512
Size: 367 1145
PasI CC'CwG_GG
Cuts at: 0 39 1512
Size: 39 1473
PflMI CCAn_nnn'nTGG
Cuts at: 0 770 1512
Size: 770 742
PpuMI rG'GwC_Cy
Cuts at: 0 600 688 1512
Size: 600 88 824
Fragments arranged by size:
824 600 88
PspOMI G'GGCC_C
Cuts at: 0 81 1512
Size: 81 1431
PstI C_TGCA'G
Cuts at: 0 345 398 926 935 1424 1512
Size: 345 53 528 9 489 88
Fragments arranged by size:
528 489 345 88 53 9
SacI G_AGCT'C
Cuts at: 0 375 1512
Size: 375 1137
SacII CC_GC'GG
Cuts at: 0 116 174 1512
Size: 116 58 1338
Fragments arranged by size:
1338 116 58
SapI GCTCTTCn'nnn_
Cuts at: 0 208 1512
Size: 208 1304
ScaI AGT'ACT
Cuts at: 0 327 1512
Size: 327 1185
SfcI C'TryA_G
Cuts at: 0 341 394 922 931 1218 1420 1512
Size: 341 53 528 9 287 202 92
Fragments arranged by size:
528 341 287 202 92 53 9
SfoI GGC'GCC
Cuts at: 0 131 1512
Size: 131 1381
SmaI CCC'GGG
Cuts at: 0 9 99 1104 1512
Size: 9 90 1005 408
Fragments arranged by size:
1005 408 90 9
SmlI C'TyrA_G
Cuts at: 0 1178 1512
Size: 1178 334
StyI C'CwwG_G
Cuts at: 0 45 781 1362 1512
Size: 45 736 581 150
Fragments arranged by size:
736 581 150 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 477 874 1512
Size: 202 275 397 638
Fragments arranged by size:
638 397 275 202
TatI w'GTAC_w
Cuts at: 0 325 1189 1512
Size: 325 864 323
Fragments arranged by size:
864 325 323
XhoI C'TCGA_G
Cuts at: 0 1178 1512
Size: 1178 334
XmaI C'CCGG_G
Cuts at: 0 7 97 1102 1512
Size: 7 90 1005 410
Fragments arranged by size:
1005 410 90 7
XmnI GAAnn'nnTTC
Cuts at: 0 974 1318 1340 1496 1512
Size: 974 344 22 156 16
Fragments arranged by size:
974 344 156 22 16
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BciVI BglI BlpI Bme1580I
BmgBI BpmI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtsI ClaI EaeI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HindIII KasI MmeI
MspA1I NarI NspI PasI PflMI PpuMI PspOMI PstI
SacI SacII SapI ScaI SfcI SfoI SmaI SmlI
StyI TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BclI BfrBI BglII BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KpnI MfeI MluI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI ZraI