Restriction map for
pF1KB9415
(Linear) (Six-base) MAPSORT of: KIBB9415.seq Check: 9945 from: 1 to: 2070
KIBB9415.orf
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AccI GT'mk_AC
Cuts at: 0 318 2070
Size: 318 1752
Acc65I G'GTAC_C
Cuts at: 0 1967 2070
Size: 1967 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 952 1184 2070
Size: 44 908 232 886
Fragments arranged by size:
908 886 232 44
AflII C'TTAA_G
Cuts at: 0 1933 2070
Size: 1933 137
AflIII A'CryG_T
Cuts at: 0 106 392 2070
Size: 106 286 1678
Fragments arranged by size:
1678 286 106
AlwNI CAG_nnn'CTG
Cuts at: 0 1674 2070
Size: 1674 396
ApaLI G'TGCA_C
Cuts at: 0 1356 2070
Size: 1356 714
ApoI r'AATT_y
Cuts at: 0 8 148 330 668 856 916 1220 1338
Size: 8 140 182 338 188 60 304 118
Cuts at: 1338 1604 2070
Size: 266 466
Fragments arranged by size:
466 338 304 266 188 182 140 118
60 8
AseI AT'TA_AT
Cuts at: 0 590 2070
Size: 590 1480
AvaI C'yCGr_G
Cuts at: 0 487 2070
Size: 487 1583
BanI G'GyrC_C
Cuts at: 0 1967 2070
Size: 1967 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 2006 2070
Size: 2006 64
BciVI GTATCCnnnnn_n'
Cuts at: 0 1063 2070
Size: 1063 1007
BglI GCCn_nnn'nGGC
Cuts at: 0 1210 1277 2036 2070
Size: 1210 67 759 34
Fragments arranged by size:
1210 759 67 34
BglII A'GATC_T
Cuts at: 0 945 2070
Size: 945 1125
BlpI GC'TnA_GC
Cuts at: 0 1158 1617 2070
Size: 1158 459 453
Fragments arranged by size:
1158 459 453
Bme1580I G_kGCm'C
Cuts at: 0 1360 2070
Size: 1360 710
BmtI G_CTAG'C
Cuts at: 0 808 2070
Size: 808 1262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 898 1755 1961 2070
Size: 898 857 206 109
Fragments arranged by size:
898 857 206 109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 2070
Size: 908 1162
Bpu10I CC'TnA_GC
Cuts at: 0 461 2070
Size: 461 1609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 2045 2070
Size: 287 1758 25
Fragments arranged by size:
1758 287 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 153 2070
Size: 153 1917
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1828 1858 2070
Size: 1828 30 212
Fragments arranged by size:
1828 212 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1639 2070
Size: 1639 431
BseYI C'CCAG_C
Cuts at: 0 957 1510 1541 2070
Size: 957 553 31 529
Fragments arranged by size:
957 553 529 31
BsiHKAI G_wGCw'C
Cuts at: 0 1360 2070
Size: 1360 710
Bsp1286I G_dGCh'C
Cuts at: 0 1360 2070
Size: 1360 710
BsrBI CCG'CTC
Cuts at: 0 1616 1844 2070
Size: 1616 228 226
Fragments arranged by size:
1616 228 226
BsrFI r'CCGG_y
Cuts at: 0 139 2070
Size: 139 1931
BsrGI T'GTAC_A
Cuts at: 0 177 2070
Size: 177 1893
BssSI C'ACGA_G
Cuts at: 0 883 2070
Size: 883 1187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1042 2070
Size: 1042 1028
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1927 2070
Size: 1927 143
BstYI r'GATC_y
Cuts at: 0 219 577 945 1294 1320 2070
Size: 219 358 368 349 26 750
Fragments arranged by size:
750 368 358 349 219 26
BstZ17I GTA'TAC
Cuts at: 0 319 2070
Size: 319 1751
BtsI GCAGTG_nn'
Cuts at: 0 1152 2070
Size: 1152 918
DraI TTT'AAA
Cuts at: 0 662 2070
Size: 662 1408
EarI CTCTTCn'nnn_
Cuts at: 0 1287 2070
Size: 1287 783
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 867 2070
Size: 867 1203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 908 952 1184 2070
Size: 44 864 44 232 886
Fragments arranged by size:
886 864 232 44 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 80 2070
Size: 80 1990
EcoO109I rG'GnC_Cy
Cuts at: 0 879 2070
Size: 879 1191
EcoRI G'AATT_C
Cuts at: 0 330 1338 2070
Size: 330 1008 732
Fragments arranged by size:
1008 732 330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 1101 1133 2070
Size: 347 32 722 32 937
Fragments arranged by size:
937 722 347 32 32
HincII GTy'rAC
Cuts at: 0 455 608 2070
Size: 455 153 1462
Fragments arranged by size:
1462 455 153
HpaI GTT'AAC
Cuts at: 0 608 2070
Size: 608 1462
KpnI G_GTAC'C
Cuts at: 0 1971 2070
Size: 1971 99
MfeI C'AATT_G
Cuts at: 0 1046 1088 2070
Size: 1046 42 982
Fragments arranged by size:
1046 982 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 2070
Size: 1669 401
MspA1I CmG'CkG
Cuts at: 0 1201 1430 1671 2070
Size: 1201 229 241 399
Fragments arranged by size:
1201 399 241 229
NdeI CA'TA_TG
Cuts at: 0 54 2070
Size: 54 2016
NheI G'CTAG_C
Cuts at: 0 804 2070
Size: 804 1266
NspI r_CATG'y
Cuts at: 0 110 276 396 906 1198 2070
Size: 110 166 120 510 292 872
Fragments arranged by size:
872 510 292 166 120 110
PciI A'CATG_T
Cuts at: 0 106 392 2070
Size: 106 286 1678
Fragments arranged by size:
1678 286 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 1007 2070
Size: 1007 1063
PpuMI rG'GwC_Cy
Cuts at: 0 879 2070
Size: 879 1191
PvuII CAG'CTG
Cuts at: 0 1201 1671 2070
Size: 1201 470 399
Fragments arranged by size:
1201 470 399
SapI GCTCTTCn'nnn_
Cuts at: 0 1287 2070
Size: 1287 783
SfcI C'TryA_G
Cuts at: 0 755 797 2070
Size: 755 42 1273
Fragments arranged by size:
1273 755 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2036 2070
Size: 2036 34
SmlI C'TyrA_G
Cuts at: 0 302 1933 2060 2070
Size: 302 1631 127 10
Fragments arranged by size:
1631 302 127 10
SphI G_CATG'C
Cuts at: 0 276 2070
Size: 276 1794
SspI AAT'ATT
Cuts at: 0 1659 2070
Size: 1659 411
StuI AGG'CCT
Cuts at: 0 1424 2070
Size: 1424 646
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1302 2070
Size: 1302 768
TatI w'GTAC_w
Cuts at: 0 177 2070
Size: 177 1893
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1683 1715 2070
Size: 1683 32 355
Fragments arranged by size:
1683 355 32
XbaI T'CTAG_A
Cuts at: 0 1449 1873 2070
Size: 1449 424 197
Fragments arranged by size:
1449 424 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1121 2070
Size: 1121 949
XmnI GAAnn'nnTTC
Cuts at: 0 8 2070
Size: 8 2062
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlwNI ApaLI ApoI
AseI AvaI BanI BbsI BciVI BglI BglII BlpI
Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BsrGI BssSI
BstAPI BstXI BstYI BstZ17I BtsI DraI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HpaI KpnI
MfeI MmeI MspA1I NdeI NheI NspI PciI PflMI
PpuMI PvuII SapI SfcI SfiI SmlI SphI SspI
StuI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvrII BaeI BamHI BanII
BbeI BbvCI BcgI BclI BfrBI BmgBI BmrI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BssHII BstBI BstEII Bsu36I
BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EcoICRI EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI MluI MscI MslI NaeI NarI NcoI NgoMIV
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StyI
SwaI Tth111I XhoI XmaI ZraI