Restriction map for
pF1KB9425
(Linear) (Six-base) MAPSORT of: KIBB9425.seq Check: 1909 from: 1 to: 2211
KIBB9425.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 622 2211
Size: 622 1589
AatII G_ACGT'C
Cuts at: 0 1328 2084 2211
Size: 1328 756 127
Fragments arranged by size:
1328 756 127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 2211
Size: 1318 893
AfeI AGC'GCT
Cuts at: 0 1498 2211
Size: 1498 713
AflII C'TTAA_G
Cuts at: 0 22 2211
Size: 22 2189
AleI CACnn'nnGTG
Cuts at: 0 214 616 2131 2211
Size: 214 402 1515 80
Fragments arranged by size:
1515 402 214 80
ApaI G_GGCC'C
Cuts at: 0 1776 2211
Size: 1776 435
ApoI r'AATT_y
Cuts at: 0 1507 2170 2211
Size: 1507 663 41
Fragments arranged by size:
1507 663 41
AvaI C'yCGr_G
Cuts at: 0 2108 2211
Size: 2108 103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 585 618 1384 1417 2211
Size: 585 33 766 33 794
Fragments arranged by size:
794 766 585 33 33
BanI G'GyrC_C
Cuts at: 0 632 976 2211
Size: 632 344 1235
Fragments arranged by size:
1235 632 344
BanII G_rGCy'C
Cuts at: 0 644 700 1165 1776 2211
Size: 644 56 465 611 435
Fragments arranged by size:
644 611 465 435 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 2204 2211
Size: 181 2023 7
Fragments arranged by size:
2023 181 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 586 748 2211
Size: 586 162 1463
Fragments arranged by size:
1463 586 162
BclI T'GATC_A
Cuts at: 0 380 2211
Size: 380 1831
BlpI GC'TnA_GC
Cuts at: 0 1888 2211
Size: 1888 323
Bme1580I G_kGCm'C
Cuts at: 0 1776 2211
Size: 1776 435
BmgBI CAC'GTC
Cuts at: 0 1347 1869 2211
Size: 1347 522 342
Fragments arranged by size:
1347 522 342
BmrI ACTGGGnnnn_n'
Cuts at: 0 1873 2025 2211
Size: 1873 152 186
Fragments arranged by size:
1873 186 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2047 2211
Size: 376 1671 164
Fragments arranged by size:
1671 376 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1264 2211
Size: 1096 168 947
Fragments arranged by size:
1096 947 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 673 847 1469 2211
Size: 673 174 622 742
Fragments arranged by size:
742 673 622 174
BsaBI GATnn'nnATC
Cuts at: 0 31 2211
Size: 31 2180
BsaHI Gr'CG_yC
Cuts at: 0 1325 1979 2081 2211
Size: 1325 654 102 130
Fragments arranged by size:
1325 654 130 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1732 1762 2211
Size: 1732 30 449
Fragments arranged by size:
1732 449 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 1130 1135 2211
Size: 322 808 5 1076
Fragments arranged by size:
1076 808 322 5
BseYI C'CCAG_C
Cuts at: 0 661 701 2211
Size: 661 40 1510
Fragments arranged by size:
1510 661 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 594 1338 2211
Size: 276 318 744 873
Fragments arranged by size:
873 744 318 276
BsiEI CG_ry'CG
Cuts at: 0 97 457 2211
Size: 97 360 1754
Fragments arranged by size:
1754 360 97
BsiHKAI G_wGCw'C
Cuts at: 0 644 1127 2024 2211
Size: 644 483 897 187
Fragments arranged by size:
897 644 483 187
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1145 2072 2211
Size: 1145 927 139
Fragments arranged by size:
1145 927 139
Bsp1286I G_dGCh'C
Cuts at: 0 644 700 1127 1165 1776 2024 2211
Size: 644 56 427 38 611 248 187
Fragments arranged by size:
644 611 427 248 187 56 38
BspHI T'CATG_A
Cuts at: 0 1922 2211
Size: 1922 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 622 2211
Size: 622 1589
BsrDI GCAATG_nn'
Cuts at: 0 122 552 968 2211
Size: 122 430 416 1243
Fragments arranged by size:
1243 430 416 122
BsrFI r'CCGG_y
Cuts at: 0 2118 2211
Size: 2118 93
BssSI C'ACGA_G
Cuts at: 0 637 2211
Size: 637 1574
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 2211
Size: 810 1401
BstXI CCAn_nnnn'nTGG
Cuts at: 0 906 1206 1576 2211
Size: 906 300 370 635
Fragments arranged by size:
906 635 370 300
BstYI r'GATC_y
Cuts at: 0 1730 2211
Size: 1730 481
BtgI C'CryG_G
Cuts at: 0 1760 2211
Size: 1760 451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1946 2211
Size: 1946 265
BtsI GCAGTG_nn'
Cuts at: 0 820 1632 2211
Size: 820 812 579
Fragments arranged by size:
820 812 579
ClaI AT'CG_AT
Cuts at: 0 386 1026 2211
Size: 386 640 1185
Fragments arranged by size:
1185 640 386
DraIII CAC_nnn'GTG
Cuts at: 0 656 2211
Size: 656 1555
DrdI GACnn_nn'nnGTC
Cuts at: 0 205 2211
Size: 205 2006
EaeI y'GGCC_r
Cuts at: 0 94 153 161 284 454 504 518 908
Size: 94 59 8 123 170 50 14 390
Cuts at: 908 948 1149 2211
Size: 40 201 1062
Fragments arranged by size:
1062 390 201 170 123 94 59 50
40 14 8
EagI C'GGCC_G
Cuts at: 0 94 454 2211
Size: 94 360 1757
Fragments arranged by size:
1757 360 94
EarI CTCTTCn'nnn_
Cuts at: 0 363 422 1363 2108 2211
Size: 363 59 941 745 103
Fragments arranged by size:
941 745 363 103 59
EcoICRI GAG'CTC
Cuts at: 0 642 2211
Size: 642 1569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1318 2047 2211
Size: 376 942 729 164
Fragments arranged by size:
942 729 376 164
EcoO109I rG'GnC_Cy
Cuts at: 0 88 753 779 1773 2211
Size: 88 665 26 994 438
Fragments arranged by size:
994 665 438 88 26
EcoRI G'AATT_C
Cuts at: 0 2170 2211
Size: 2170 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2067 2211
Size: 2067 144
HaeII r_GCGC'y
Cuts at: 0 1206 1500 1947 2211
Size: 1206 294 447 264
Fragments arranged by size:
1206 447 294 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 372 404 551 583 1365 1397
Size: 218 32 122 32 147 32 782 32
Cuts at: 1397 1534 1566 1917 1949 2211
Size: 137 32 351 32 262
Fragments arranged by size:
782 351 262 218 147 137 122 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 501 717 2211
Size: 501 216 1494
Fragments arranged by size:
1494 501 216
HindIII A'AGCT_T
Cuts at: 0 1916 2211
Size: 1916 295
MfeI C'AATT_G
Cuts at: 0 2144 2211
Size: 2144 67
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1724 2211
Size: 1724 487
MscI TGG'CCA
Cuts at: 0 286 520 1151 2211
Size: 286 234 631 1060
Fragments arranged by size:
1060 631 286 234
MslI CAynn'nnrTG
Cuts at: 0 214 616 1234 1574 2131 2211
Size: 214 402 618 340 557 80
Fragments arranged by size:
618 557 402 340 214 80
MspA1I CmG'CkG
Cuts at: 0 705 947 1155 2211
Size: 705 242 208 1056
Fragments arranged by size:
1056 705 242 208
NspI r_CATG'y
Cuts at: 0 453 723 2211
Size: 453 270 1488
Fragments arranged by size:
1488 453 270
PasI CC'CwG_GG
Cuts at: 0 1776 2211
Size: 1776 435
PflMI CCAn_nnn'nTGG
Cuts at: 0 65 84 2211
Size: 65 19 2127
Fragments arranged by size:
2127 65 19
PfoI T'CCnGG_A
Cuts at: 0 2024 2211
Size: 2024 187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 2211
Size: 421 32 1758
Fragments arranged by size:
1758 421 32
PpuMI rG'GwC_Cy
Cuts at: 0 88 753 779 2211
Size: 88 665 26 1432
Fragments arranged by size:
1432 665 88 26
PspOMI G'GGCC_C
Cuts at: 0 1772 2211
Size: 1772 439
PstI C_TGCA'G
Cuts at: 0 320 1542 1739 2211
Size: 320 1222 197 472
Fragments arranged by size:
1222 472 320 197
PvuII CAG'CTG
Cuts at: 0 1155 2211
Size: 1155 1056
SacI G_AGCT'C
Cuts at: 0 644 2211
Size: 644 1567
SanDI GG'GwC_CC
Cuts at: 0 88 779 2211
Size: 88 691 1432
Fragments arranged by size:
1432 691 88
SapI GCTCTTCn'nnn_
Cuts at: 0 422 2108 2211
Size: 422 1686 103
Fragments arranged by size:
1686 422 103
SbfI CC_TGCA'GG
Cuts at: 0 1739 2211
Size: 1739 472
ScaI AGT'ACT
Cuts at: 0 905 2211
Size: 905 1306
SexAI A'CCwGG_T
Cuts at: 0 689 2211
Size: 689 1522
SfcI C'TryA_G
Cuts at: 0 316 1538 1587 1735 2211
Size: 316 1222 49 148 476
Fragments arranged by size:
1222 476 316 148 49
SmaI CCC'GGG
Cuts at: 0 2110 2211
Size: 2110 101
SmlI C'TyrA_G
Cuts at: 0 22 1075 1279 2211
Size: 22 1053 204 932
Fragments arranged by size:
1053 932 204 22
SphI G_CATG'C
Cuts at: 0 453 2211
Size: 453 1758
StyI C'CwwG_G
Cuts at: 0 1433 2211
Size: 1433 778
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1056 2007 2009 2211
Size: 1056 951 2 202
Fragments arranged by size:
1056 951 202 2
TatI w'GTAC_w
Cuts at: 0 903 2211
Size: 903 1308
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 2211
Size: 763 32 1416
Fragments arranged by size:
1416 763 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 2211
Size: 876 1335
XmaI C'CCGG_G
Cuts at: 0 2108 2211
Size: 2108 103
XmnI GAAnn'nnTTC
Cuts at: 0 1670 1918 2211
Size: 1670 248 293
Fragments arranged by size:
1670 293 248
ZraI GAC'GTC
Cuts at: 0 1326 2082 2211
Size: 1326 756 129
Fragments arranged by size:
1326 756 129
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AleI ApaI ApoI
AvaI BaeI BanI BanII BbsI BciVI BclI BlpI
Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI ScaI SexAI SfcI SmaI
SmlI SphI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflIII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbvCI BcgI BfrBI BglI BglII BmtI BplI Bpu10I
BsaAI BsaWI BsiWI BsmI BspEI BsrBI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I CspCI DraI EciI EcoNI
EcoRV FseI FspI FspAI HpaI KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI