Restriction map for
pF1KB9429
(Linear) (Six-base) MAPSORT of: KIBB9429.seq Check: 8274 from: 1 to: 1680
KIBB9429.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 1082 1680
Size: 1082 598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 429 859 1084 1369 1680
Size: 92 337 430 225 285 311
Fragments arranged by size:
430 337 311 285 225 92
AfeI AGC'GCT
Cuts at: 0 1063 1240 1680
Size: 1063 177 440
Fragments arranged by size:
1063 440 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1490 1680
Size: 1490 190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 1680
Size: 1301 32 347
Fragments arranged by size:
1301 347 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 267 1680
Size: 18 249 1413
Fragments arranged by size:
1413 249 18
ApoI r'AATT_y
Cuts at: 0 475 808 1680
Size: 475 333 872
Fragments arranged by size:
872 475 333
AvaI C'yCGr_G
Cuts at: 0 82 1680
Size: 82 1598
BamHI G'GATC_C
Cuts at: 0 1437 1680
Size: 1437 243
BanI G'GyrC_C
Cuts at: 0 1156 1581 1680
Size: 1156 425 99
Fragments arranged by size:
1156 425 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 527 566 590 865 1429 1680
Size: 254 273 39 24 275 564 251
Fragments arranged by size:
564 275 273 254 251 39 24
BbvCI CC'TCA_GC
Cuts at: 0 374 1680
Size: 374 1306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 383 1680
Size: 349 34 1297
Fragments arranged by size:
1297 349 34
BclI T'GATC_A
Cuts at: 0 393 1680
Size: 393 1287
BlpI GC'TnA_GC
Cuts at: 0 1280 1680
Size: 1280 400
Bme1580I G_kGCm'C
Cuts at: 0 822 1680
Size: 822 858
BmrI ACTGGGnnnn_n'
Cuts at: 0 343 1150 1680
Size: 343 807 530
Fragments arranged by size:
807 530 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 361 614 1048 1680
Size: 332 29 253 434 632
Fragments arranged by size:
632 434 332 253 29
Bpu10I CC'TnA_GC
Cuts at: 0 374 1268 1558 1680
Size: 374 894 290 122
Fragments arranged by size:
894 374 290 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1680
Size: 1310 370
BsaWI w'CCGG_w
Cuts at: 0 1311 1680
Size: 1311 369
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 526 529 964 1680
Size: 526 3 435 716
Fragments arranged by size:
716 526 435 3
BseYI C'CCAG_C
Cuts at: 0 1045 1680
Size: 1045 635
Bsp1286I G_dGCh'C
Cuts at: 0 822 1680
Size: 822 858
BsrBI CCG'CTC
Cuts at: 0 1634 1680
Size: 1634 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 267 1680
Size: 267 1413
BstBI TT'CG_AA
Cuts at: 0 1221 1680
Size: 1221 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 1053 1434 1680
Size: 489 564 381 246
Fragments arranged by size:
564 489 381 246
BstYI r'GATC_y
Cuts at: 0 1316 1437 1680
Size: 1316 121 243
Fragments arranged by size:
1316 243 121
BtgI C'CryG_G
Cuts at: 0 1457 1671 1680
Size: 1457 214 9
Fragments arranged by size:
1457 214 9
BtsI GCAGTG_nn'
Cuts at: 0 92 513 1474 1680
Size: 92 421 961 206
Fragments arranged by size:
961 421 206 92
DrdI GACnn_nn'nnGTC
Cuts at: 0 1078 1680
Size: 1078 602
EaeI y'GGCC_r
Cuts at: 0 1454 1644 1680
Size: 1454 190 36
Fragments arranged by size:
1454 190 36
EarI CTCTTCn'nnn_
Cuts at: 0 406 521 666 875 977 1680
Size: 406 115 145 209 102 703
Fragments arranged by size:
703 406 209 145 115 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 1680
Size: 79 1601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 332 361 429 614 859 1048 1084
Size: 92 240 29 68 185 245 189 36
Cuts at: 1084 1369 1680
Size: 285 311
Fragments arranged by size:
311 285 245 240 189 185 92 68
36 29
EcoO109I rG'GnC_Cy
Cuts at: 0 1008 1680
Size: 1008 672
EcoRV GAT'ATC
Cuts at: 0 913 1680
Size: 913 767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 904 1680
Size: 904 776
HaeII r_GCGC'y
Cuts at: 0 25 1065 1242 1680
Size: 25 1040 177 438
Fragments arranged by size:
1040 438 177 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 292 309 324 1308 1340 1680
Size: 277 15 17 15 984 32 340
Fragments arranged by size:
984 340 277 32 17 15 15
HincII GTy'rAC
Cuts at: 0 746 1680
Size: 746 934
MscI TGG'CCA
Cuts at: 0 1456 1646 1680
Size: 1456 190 34
Fragments arranged by size:
1456 190 34
NcoI C'CATG_G
Cuts at: 0 1457 1680
Size: 1457 223
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 1680
Size: 711 969
PpuMI rG'GwC_Cy
Cuts at: 0 1008 1680
Size: 1008 672
SanDI GG'GwC_CC
Cuts at: 0 1008 1680
Size: 1008 672
SapI GCTCTTCn'nnn_
Cuts at: 0 406 666 1680
Size: 406 260 1014
Fragments arranged by size:
1014 406 260
SfcI C'TryA_G
Cuts at: 0 1083 1680
Size: 1083 597
SmlI C'TyrA_G
Cuts at: 0 1325 1680
Size: 1325 355
StyI C'CwwG_G
Cuts at: 0 482 498 567 1433 1457 1680
Size: 482 16 69 866 24 223
Fragments arranged by size:
866 482 223 69 24 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 1680
Size: 355 1325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 662 694 1680
Size: 370 32 260 32 986
Fragments arranged by size:
986 370 260 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 335 1680
Size: 335 1345
XbaI T'CTAG_A
Cuts at: 0 229 1680
Size: 229 1451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1449 1680
Size: 1449 231
Enzymes that do cut:
AccI AcuI AfeI AlfI AloI AlwNI ApoI AvaI
BamHI BanI BbsI BbvCI BcgI BclI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaWI BseRI BseYI Bsp1286I
BsrBI BstAPI BstBI BstXI BstYI BtgI BtsI DrdI
EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII MscI NcoI PflMI PpuMI SanDI SapI
SfcI SmlI StyI TaqII TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanII BbeI BciVI BfrBI BglI BglII BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XhoI XmaI XmnI ZraI