Restriction map for
pF1KB9430
(Linear) (Six-base) MAPSORT of: KIBB9430.seq Check: 5413 from: 1 to: 1299
KIBB9430.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 887 1299
Size: 887 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1125 1299
Size: 1125 174
AfeI AGC'GCT
Cuts at: 0 38 839 1299
Size: 38 801 460
Fragments arranged by size:
801 460 38
AflIII A'CryG_T
Cuts at: 0 1007 1299
Size: 1007 292
AlwNI CAG_nnn'CTG
Cuts at: 0 691 1299
Size: 691 608
AvaI C'yCGr_G
Cuts at: 0 63 76 144 162 849 1050 1231 1251
Size: 63 13 68 18 687 201 181 20
Cuts at: 1251 1264 1299
Size: 13 35
Fragments arranged by size:
687 201 181 68 63 35 20 18
13 13
BanI G'GyrC_C
Cuts at: 0 67 562 751 1299
Size: 67 495 189 548
Fragments arranged by size:
548 495 189 67
BanII G_rGCy'C
Cuts at: 0 58 1057 1190 1299
Size: 58 999 133 109
Fragments arranged by size:
999 133 109 58
BbeI G_GCGC'C
Cuts at: 0 71 566 755 1299
Size: 71 495 189 544
Fragments arranged by size:
544 495 189 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1299
Size: 1088 211
BglI GCCn_nnn'nGGC
Cuts at: 0 500 721 817 1299
Size: 500 221 96 482
Fragments arranged by size:
500 482 221 96
BglII A'GATC_T
Cuts at: 0 263 1299
Size: 263 1036
Bme1580I G_kGCm'C
Cuts at: 0 173 910 1299
Size: 173 737 389
Fragments arranged by size:
737 389 173
BmgBI CAC'GTC
Cuts at: 0 911 1299
Size: 911 388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1209 1266 1279 1299
Size: 1209 57 13 20
Fragments arranged by size:
1209 57 20 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1299
Size: 405 894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1299
Size: 334 965
BsaHI Gr'CG_yC
Cuts at: 0 68 563 752 1299
Size: 68 495 189 547
Fragments arranged by size:
547 495 189 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 409 433 439 455 485 997 1027
Size: 403 6 24 6 16 30 512 30
Cuts at: 1027 1299
Size: 272
Fragments arranged by size:
512 403 272 30 30 24 16 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 417 432 551 804 1005 1023 1026
Size: 84 333 15 119 253 201 18 3
Cuts at: 1026 1035 1038 1047 1299
Size: 9 3 9 252
Fragments arranged by size:
333 253 252 201 119 84 18 15
9 9 3 3
BseYI C'CCAG_C
Cuts at: 0 81 276 793 1105 1299
Size: 81 195 517 312 194
Fragments arranged by size:
517 312 195 194 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1209 1299
Size: 25 1184 90
Fragments arranged by size:
1184 90 25
BsiEI CG_ry'CG
Cuts at: 0 434 933 1164 1299
Size: 434 499 231 135
Fragments arranged by size:
499 434 231 135
BsiHKAI G_wGCw'C
Cuts at: 0 58 1299
Size: 58 1241
Bsp1286I G_dGCh'C
Cuts at: 0 58 173 910 1057 1190 1299
Size: 58 115 737 147 133 109
Fragments arranged by size:
737 147 133 115 109 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1097 1299
Size: 1097 202
BsrBI CCG'CTC
Cuts at: 0 219 339 935 1299
Size: 219 120 596 364
Fragments arranged by size:
596 364 219 120
BsrFI r'CCGG_y
Cuts at: 0 46 70 383 576 928 1299
Size: 46 24 313 193 352 371
Fragments arranged by size:
371 352 313 193 46 24
BsrGI T'GTAC_A
Cuts at: 0 776 1299
Size: 776 523
BssHII G'CGCG_C
Cuts at: 0 969 995 1299
Size: 969 26 304
Fragments arranged by size:
969 304 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 691 1299
Size: 691 608
BstBI TT'CG_AA
Cuts at: 0 1079 1299
Size: 1079 220
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 1299
Size: 276 1023
BstYI r'GATC_y
Cuts at: 0 263 314 1299
Size: 263 51 985
Fragments arranged by size:
985 263 51
BtgI C'CryG_G
Cuts at: 0 122 495 951 1299
Size: 122 373 456 348
Fragments arranged by size:
456 373 348 122
BtsI GCAGTG_nn'
Cuts at: 0 486 1299
Size: 486 813
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 456 491 1299
Size: 456 35 808
Fragments arranged by size:
808 456 35
EaeI y'GGCC_r
Cuts at: 0 44 431 492 930 1299
Size: 44 387 61 438 369
Fragments arranged by size:
438 387 369 61 44
EagI C'GGCC_G
Cuts at: 0 431 930 1299
Size: 431 499 369
Fragments arranged by size:
499 431 369
EarI CTCTTCn'nnn_
Cuts at: 0 1044 1299
Size: 1044 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 798 880 1299
Size: 349 449 82 419
Fragments arranged by size:
449 419 349 82
EcoICRI GAG'CTC
Cuts at: 0 56 1299
Size: 56 1243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1125 1299
Size: 405 720 174
Fragments arranged by size:
720 405 174
EcoO109I rG'GnC_Cy
Cuts at: 0 1167 1299
Size: 1167 132
HaeII r_GCGC'y
Cuts at: 0 40 71 566 755 800 841 1159 1299
Size: 40 31 495 189 45 41 318 140
Fragments arranged by size:
495 318 189 140 45 41 40 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 754 786 1111 1143 1299
Size: 455 32 267 32 325 32 156
Fragments arranged by size:
455 325 267 156 32 32 32
KasI G'GCGC_C
Cuts at: 0 67 562 751 1299
Size: 67 495 189 548
Fragments arranged by size:
548 495 189 67
MluI A'CGCG_T
Cuts at: 0 1007 1299
Size: 1007 292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1299
Size: 1091 208
MspA1I CmG'CkG
Cuts at: 0 688 953 1299
Size: 688 265 346
Fragments arranged by size:
688 346 265
NaeI GCC'GGC
Cuts at: 0 48 72 930 1299
Size: 48 24 858 369
Fragments arranged by size:
858 369 48 24
NarI GG'CG_CC
Cuts at: 0 68 563 752 1299
Size: 68 495 189 547
Fragments arranged by size:
547 495 189 68
NcoI C'CATG_G
Cuts at: 0 495 1299
Size: 495 804
NgoMIV G'CCGG_C
Cuts at: 0 46 70 928 1299
Size: 46 24 858 371
Fragments arranged by size:
858 371 46 24
NspI r_CATG'y
Cuts at: 0 252 1128 1299
Size: 252 876 171
Fragments arranged by size:
876 252 171
PasI CC'CwG_GG
Cuts at: 0 747 1131 1299
Size: 747 384 168
Fragments arranged by size:
747 384 168
PfoI T'CCnGG_A
Cuts at: 0 1214 1299
Size: 1214 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1299
Size: 1093 32 174
Fragments arranged by size:
1093 174 32
PpuMI rG'GwC_Cy
Cuts at: 0 1167 1299
Size: 1167 132
PspXI vC'TCGA_Gb
Cuts at: 0 76 1264 1299
Size: 76 1188 35
Fragments arranged by size:
1188 76 35
SacI G_AGCT'C
Cuts at: 0 58 1299
Size: 58 1241
SacII CC_GC'GG
Cuts at: 0 954 1299
Size: 954 345
SexAI A'CCwGG_T
Cuts at: 0 1283 1299
Size: 1283 16
SfoI GGC'GCC
Cuts at: 0 69 564 753 1299
Size: 69 495 189 546
Fragments arranged by size:
546 495 189 69
SmaI CCC'GGG
Cuts at: 0 851 1299
Size: 851 448
SmlI C'TyrA_G
Cuts at: 0 76 349 1264 1299
Size: 76 273 915 35
Fragments arranged by size:
915 273 76 35
StyI C'CwwG_G
Cuts at: 0 495 1299
Size: 495 804
TatI w'GTAC_w
Cuts at: 0 776 1299
Size: 776 523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 997 1029 1299
Size: 997 32 270
Fragments arranged by size:
997 270 32
Tth111I GACn'n_nGTC
Cuts at: 0 469 1299
Size: 469 830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 1299
Size: 654 645
XhoI C'TCGA_G
Cuts at: 0 76 1264 1299
Size: 76 1188 35
Fragments arranged by size:
1188 76 35
XmaI C'CCGG_G
Cuts at: 0 849 1299
Size: 849 450
XmnI GAAnn'nnTTC
Cuts at: 0 193 1299
Size: 193 1106
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BmgBI BplI BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstXI BstYI BtgI BtsI CspCI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PfoI PpiI PpuMI PspXI SacI SacII SexAI SfoI
SmaI SmlI StyI TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MscI MslI NdeI NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
XbaI ZraI