Restriction map for
pF1KB9431
(Linear) (Six-base) MAPSORT of: KIBB9431.seq Check: 3470 from: 1 to: 1572
KIBB9431.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 121 352 1572
Size: 121 231 1220
Fragments arranged by size:
1220 231 121
AccI GT'mk_AC
Cuts at: 0 1259 1572
Size: 1259 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 995 1159 1559 1572
Size: 439 556 164 400 13
Fragments arranged by size:
556 439 400 164 13
AgeI A'CCGG_T
Cuts at: 0 839 1572
Size: 839 733
AleI CACnn'nnGTG
Cuts at: 0 1426 1572
Size: 1426 146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 623 1572
Size: 623 949
AlwNI CAG_nnn'CTG
Cuts at: 0 588 636 1484 1572
Size: 588 48 848 88
Fragments arranged by size:
848 588 88 48
ApaI G_GGCC'C
Cuts at: 0 1411 1572
Size: 1411 161
AvaI C'yCGr_G
Cuts at: 0 59 1303 1572
Size: 59 1244 269
Fragments arranged by size:
1244 269 59
BanI G'GyrC_C
Cuts at: 0 1327 1572
Size: 1327 245
BanII G_rGCy'C
Cuts at: 0 899 1241 1312 1339 1411 1478 1571 1572
Size: 899 342 71 27 72 67 93 1
Fragments arranged by size:
899 342 93 72 71 67 27 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 1052 1559 1572
Size: 515 537 507 13
Fragments arranged by size:
537 515 507 13
BbvCI CC'TCA_GC
Cuts at: 0 1040 1572
Size: 1040 532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1098 1132 1572
Size: 1098 34 440
Fragments arranged by size:
1098 440 34
BclI T'GATC_A
Cuts at: 0 144 1572
Size: 144 1428
BglI GCCn_nnn'nGGC
Cuts at: 0 1102 1572
Size: 1102 470
Bme1580I G_kGCm'C
Cuts at: 0 923 1411 1572
Size: 923 488 161
Fragments arranged by size:
923 488 161
BmrI ACTGGGnnnn_n'
Cuts at: 0 1472 1572
Size: 1472 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1572
Size: 1189 383
Bpu10I CC'TnA_GC
Cuts at: 0 758 1040 1572
Size: 758 282 532
Fragments arranged by size:
758 532 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 690 1444 1572
Size: 690 754 128
Fragments arranged by size:
754 690 128
BsaHI Gr'CG_yC
Cuts at: 0 65 326 791 1510 1572
Size: 65 261 465 719 62
Fragments arranged by size:
719 465 261 65 62
BsaWI w'CCGG_w
Cuts at: 0 839 1572
Size: 839 733
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 1572
Size: 358 30 1184
Fragments arranged by size:
1184 358 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 757 919 952 955 958 961 964
Size: 472 285 162 33 3 3 3 3
Cuts at: 964 967 970 973 997 1000 1003 1006 1135
Size: 3 3 3 24 3 3 3 129
Cuts at: 1135 1165 1572
Size: 30 407
Fragments arranged by size:
472 407 285 162 129 33 30 24
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 35 490 1572
Size: 35 455 1082
Fragments arranged by size:
1082 455 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 1027 1572
Size: 213 814 545
Fragments arranged by size:
814 545 213
BsiEI CG_ry'CG
Cuts at: 0 810 1135 1572
Size: 810 325 437
Fragments arranged by size:
810 437 325
BsiHKAI G_wGCw'C
Cuts at: 0 1241 1571 1572
Size: 1241 330 1
Fragments arranged by size:
1241 330 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 72 1572
Size: 72 1500
Bsp1286I G_dGCh'C
Cuts at: 0 899 923 1241 1312 1339 1411 1478 1571
Size: 899 24 318 71 27 72 67 93
Cuts at: 1571 1572
Size: 1
Fragments arranged by size:
899 318 93 72 71 67 27 24
1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 352 1572
Size: 121 231 1220
Fragments arranged by size:
1220 231 121
BsrBI CCG'CTC
Cuts at: 0 1131 1418 1572
Size: 1131 287 154
Fragments arranged by size:
1131 287 154
BsrFI r'CCGG_y
Cuts at: 0 839 1026 1572
Size: 839 187 546
Fragments arranged by size:
839 546 187
BsrGI T'GTAC_A
Cuts at: 0 1220 1572
Size: 1220 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 877 1397 1572
Size: 877 520 175
Fragments arranged by size:
877 520 175
BstEII G'GTnAC_C
Cuts at: 0 396 1458 1572
Size: 396 1062 114
Fragments arranged by size:
1062 396 114
BstYI r'GATC_y
Cuts at: 0 425 1572
Size: 425 1147
BtgI C'CryG_G
Cuts at: 0 41 1454 1572
Size: 41 1413 118
Fragments arranged by size:
1413 118 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 314 1572
Size: 314 1258
BtsI GCAGTG_nn'
Cuts at: 0 225 850 1572
Size: 225 625 722
Fragments arranged by size:
722 625 225
ClaI AT'CG_AT
Cuts at: 0 1088 1572
Size: 1088 484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1305 1340 1572
Size: 1305 35 232
Fragments arranged by size:
1305 232 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 682 1572
Size: 682 890
EaeI y'GGCC_r
Cuts at: 0 128 389 1028 1572
Size: 128 261 639 544
Fragments arranged by size:
639 544 261 128
EarI CTCTTCn'nnn_
Cuts at: 0 590 962 974 1572
Size: 590 372 12 598
Fragments arranged by size:
598 590 372 12
EcoICRI GAG'CTC
Cuts at: 0 1239 1569 1572
Size: 1239 330 3
Fragments arranged by size:
1239 330 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 995 1159 1189 1559 1572
Size: 439 556 164 30 370 13
Fragments arranged by size:
556 439 370 164 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 1572
Size: 433 1139
EcoO109I rG'GnC_Cy
Cuts at: 0 56 762 1407 1408 1572
Size: 56 706 645 1 164
Fragments arranged by size:
706 645 164 56 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 781 813 1572
Size: 358 32 391 32 759
Fragments arranged by size:
759 391 358 32 32
HincII GTy'rAC
Cuts at: 0 153 276 1572
Size: 153 123 1296
Fragments arranged by size:
1296 153 123
HindIII A'AGCT_T
Cuts at: 0 1279 1572
Size: 1279 293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 1572
Size: 290 1282
MslI CAynn'nnrTG
Cuts at: 0 1426 1572
Size: 1426 146
MspA1I CmG'CkG
Cuts at: 0 12 323 603 1572
Size: 12 311 280 969
Fragments arranged by size:
969 311 280 12
NaeI GCC'GGC
Cuts at: 0 1028 1572
Size: 1028 544
NcoI C'CATG_G
Cuts at: 0 1454 1572
Size: 1454 118
NgoMIV G'CCGG_C
Cuts at: 0 1026 1572
Size: 1026 546
PflMI CCAn_nnn'nTGG
Cuts at: 0 35 1572
Size: 35 1537
PspOMI G'GGCC_C
Cuts at: 0 1407 1572
Size: 1407 165
PstI C_TGCA'G
Cuts at: 0 383 704 1572
Size: 383 321 868
Fragments arranged by size:
868 383 321
PvuII CAG'CTG
Cuts at: 0 603 1572
Size: 603 969
RsrII CG'GwC_CG
Cuts at: 0 842 1572
Size: 842 730
SacI G_AGCT'C
Cuts at: 0 1241 1571 1572
Size: 1241 330 1
Fragments arranged by size:
1241 330 1
SapI GCTCTTCn'nnn_
Cuts at: 0 590 1572
Size: 590 982
SexAI A'CCwGG_T
Cuts at: 0 512 1572
Size: 512 1060
SfcI C'TryA_G
Cuts at: 0 379 484 700 1572
Size: 379 105 216 872
Fragments arranged by size:
872 379 216 105
SmaI CCC'GGG
Cuts at: 0 61 1572
Size: 61 1511
StuI AGG'CCT
Cuts at: 0 1081 1572
Size: 1081 491
StyI C'CwwG_G
Cuts at: 0 614 899 1323 1454 1572
Size: 614 285 424 131 118
Fragments arranged by size:
614 424 285 131 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1560 1572
Size: 1560 12
TatI w'GTAC_w
Cuts at: 0 314 1220 1572
Size: 314 906 352
Fragments arranged by size:
906 352 314
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 849 881 1572
Size: 849 32 691
Fragments arranged by size:
849 691 32
Tth111I GACn'n_nGTC
Cuts at: 0 811 1572
Size: 811 761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1164 1572
Size: 1164 408
XmaI C'CCGG_G
Cuts at: 0 59 1572
Size: 59 1513
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlfI AlwNI ApaI
AvaI BanI BanII BbsI BbvCI BcgI BclI BglI
Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstAPI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HindIII MmeI MslI MspA1I
NaeI NcoI NgoMIV PflMI PspOMI PstI PvuII RsrII
SacI SapI SexAI SfcI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BglII BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsiWI BsmI BspEI BspHI BsrDI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I DraI DraIII
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI