Restriction map for
pF1KB9434
(Linear) (Six-base) MAPSORT of: KIBB9434.seq Check: 7575 from: 1 to: 648
KIBB9434.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 559 648
Size: 559 89
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 151 427 648
Size: 34 117 276 221
Fragments arranged by size:
276 221 117 34
AfeI AGC'GCT
Cuts at: 0 332 648
Size: 332 316
AflIII A'CryG_T
Cuts at: 0 186 409 648
Size: 186 223 239
Fragments arranged by size:
239 223 186
ApaI G_GGCC'C
Cuts at: 0 280 648
Size: 280 368
ApoI r'AATT_y
Cuts at: 0 58 648
Size: 58 590
AvaI C'yCGr_G
Cuts at: 0 117 648
Size: 117 531
BanI G'GyrC_C
Cuts at: 0 226 648
Size: 226 422
BanII G_rGCy'C
Cuts at: 0 102 280 648
Size: 102 178 368
Fragments arranged by size:
368 178 102
BglII A'GATC_T
Cuts at: 0 446 648
Size: 446 202
Bme1580I G_kGCm'C
Cuts at: 0 280 648
Size: 280 368
BmgBI CAC'GTC
Cuts at: 0 492 648
Size: 492 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 183 339 648
Size: 146 37 156 309
Fragments arranged by size:
309 156 146 37
Bpu10I CC'TnA_GC
Cuts at: 0 101 648
Size: 101 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 648
Size: 295 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 163 228 551 648
Size: 73 90 65 323 97
Fragments arranged by size:
323 97 90 73 65
BsiEI CG_ry'CG
Cuts at: 0 513 648
Size: 513 135
Bsp1286I G_dGCh'C
Cuts at: 0 102 280 648
Size: 102 178 368
Fragments arranged by size:
368 178 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 9 559 648
Size: 9 550 89
Fragments arranged by size:
550 89 9
BsrBI CCG'CTC
Cuts at: 0 252 648
Size: 252 396
BstYI r'GATC_y
Cuts at: 0 446 458 648
Size: 446 12 190
Fragments arranged by size:
446 190 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 395 430 648
Size: 395 35 218
Fragments arranged by size:
395 218 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 151 427 648
Size: 34 117 276 221
Fragments arranged by size:
276 221 117 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 16 648
Size: 16 632
EcoO109I rG'GnC_Cy
Cuts at: 0 276 277 648
Size: 276 1 371
Fragments arranged by size:
371 276 1
HaeII r_GCGC'y
Cuts at: 0 334 648
Size: 334 314
HincII GTy'rAC
Cuts at: 0 546 648
Size: 546 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 430 648
Size: 69 361 218
Fragments arranged by size:
361 218 69
MslI CAynn'nnrTG
Cuts at: 0 523 583 648
Size: 523 60 65
Fragments arranged by size:
523 65 60
NspI r_CATG'y
Cuts at: 0 190 413 648
Size: 190 223 235
Fragments arranged by size:
235 223 190
PciI A'CATG_T
Cuts at: 0 186 409 648
Size: 186 223 239
Fragments arranged by size:
239 223 186
PspOMI G'GGCC_C
Cuts at: 0 276 648
Size: 276 372
SmlI C'TyrA_G
Cuts at: 0 310 648
Size: 310 338
SspI AAT'ATT
Cuts at: 0 401 648
Size: 401 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 406 517 648
Size: 165 241 111 131
Fragments arranged by size:
241 165 131 111
TatI w'GTAC_w
Cuts at: 0 183 347 648
Size: 183 164 301
Fragments arranged by size:
301 183 164
Tth111I GACn'n_nGTC
Cuts at: 0 514 648
Size: 514 134
XmnI GAAnn'nnTTC
Cuts at: 0 401 648
Size: 401 247
Enzymes that do cut:
AarI AcuI AfeI AflIII ApaI ApoI AvaI BanI
BanII BglII Bme1580I BmgBI BmrI Bpu10I BpuEI BsgI
BsiEI Bsp1286I BspMI BsrBI BstYI CspCI Eco57MI EcoNI
EcoO109I HaeII HincII MmeI MslI NspI PciI PspOMI
SmlI SspI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmtI BplI BpmI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI
TstI XbaI XcmI XhoI XmaI ZraI