(Linear) (Six-base) MAPSORT of: KIBB9436.seq  Check: 5855  from: 1  to: 438



KIBB9436.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     168     438 
   Size:        168     270
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     258     351     438 
   Size:         69     189      93      87

  Fragments arranged by size: 

                 189      93      87      69
 
BanI G'GyrC_C

Cuts at:      0     141     438 
   Size:        141     297
 
BanII G_rGCy'C

Cuts at:      0     331     438 
   Size:        331     107
 
BbeI G_GCGC'C

Cuts at:      0     145     438 
   Size:        145     293
 
BbvCI CC'TCA_GC

Cuts at:      0      98     438 
   Size:         98     340
 
BfrBI ATG'CAT

Cuts at:      0     224     438 
   Size:        224     214
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     204     438 
   Size:        204     234
 
Bpu10I CC'TnA_GC

Cuts at:      0      98     325     438 
   Size:         98     227     113

  Fragments arranged by size: 

                 227     113      98
 
BsaHI Gr'CG_yC

Cuts at:      0     142     438 
   Size:        142     296
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      86     438 
   Size:         86     352
 
BseYI C'CCAG_C

Cuts at:      0      10     438 
   Size:         10     428
 
Bsp1286I G_dGCh'C

Cuts at:      0     331     438 
   Size:        331     107
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     168     438 
   Size:        168     270
 
BsrFI r'CCGG_y

Cuts at:      0     108     138     438 
   Size:        108      30     300

  Fragments arranged by size: 

                 300     108      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     351     438 
   Size:        351      87
 
BstBI TT'CG_AA

Cuts at:      0     422     438 
   Size:        422      16
 
BtgI C'CryG_G

Cuts at:      0     145     438 
   Size:        145     293
 
EarI CTCTTCn'nnn_

Cuts at:      0     173     438 
   Size:        173     265
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      49     438 
   Size:         49     389
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     438 
   Size:        255     183
 
FspI TGC'GCA

Cuts at:      0     292     438 
   Size:        292     146
 
HaeII r_GCGC'y

Cuts at:      0     145     438 
   Size:        145     293
 
KasI G'GCGC_C

Cuts at:      0     141     438 
   Size:        141     297
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     438 
   Size:         83     355
 
MspA1I CmG'CkG

Cuts at:      0      69      87     351     438 
   Size:         69      18     264      87

  Fragments arranged by size: 

                 264      87      69      18
 
NaeI GCC'GGC

Cuts at:      0     140     438 
   Size:        140     298
 
NarI GG'CG_CC

Cuts at:      0     142     438 
   Size:        142     296
 
NgoMIV G'CCGG_C

Cuts at:      0     138     438 
   Size:        138     300
 
NsiI A_TGCA'T

Cuts at:      0     226     438 
   Size:        226     212
 
NspI r_CATG'y

Cuts at:      0     420     438 
   Size:        420      18
 
PfoI T'CCnGG_A

Cuts at:      0     281     362     438 
   Size:        281      81      76

  Fragments arranged by size: 

                 281      81      76
 
PpuMI rG'GwC_Cy

Cuts at:      0     255     438 
   Size:        255     183
 
PstI C_TGCA'G

Cuts at:      0     356     438 
   Size:        356      82
 
PvuII CAG'CTG

Cuts at:      0     351     438 
   Size:        351      87
 
SfcI C'TryA_G

Cuts at:      0     352     438 
   Size:        352      86
 
SfoI GGC'GCC

Cuts at:      0     143     438 
   Size:        143     295
 
SgrAI Cr'CCGG_yG

Cuts at:      0     138     438 
   Size:        138     300
 
StyI C'CwwG_G

Cuts at:      0     431     438 
   Size:        431       7
 
TatI w'GTAC_w

Cuts at:      0     401     438 
   Size:        401      37
 

 Enzymes that do cut:

     AarI    AlwNI     BanI    BanII     BbeI    BbvCI    BfrBI     BplI
   Bpu10I    BsaHI    BseRI    BseYI Bsp1286I    BspMI    BsrFI   BstAPI
    BstBI     BtgI     EarI     EciI EcoO109I     FspI    HaeII     KasI
     MmeI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI     PfoI
    PpuMI     PstI    PvuII     SfcI     SfoI    SgrAI     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI