Restriction map for
pF1KB9443
(Linear) (Six-base) MAPSORT of: KIBB9443.seq Check: 9963 from: 1 to: 921
KIBB9443.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 277 517 886 921
Size: 142 135 240 369 35
Fragments arranged by size:
369 240 142 135 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 842 921
Size: 842 79
AvaI C'yCGr_G
Cuts at: 0 342 921
Size: 342 579
AvrII C'CTAG_G
Cuts at: 0 906 921
Size: 906 15
BanII G_rGCy'C
Cuts at: 0 364 652 665 808 921
Size: 364 288 13 143 113
Fragments arranged by size:
364 288 143 113 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 597 846 921
Size: 597 249 75
Fragments arranged by size:
597 249 75
BglI GCCn_nnn'nGGC
Cuts at: 0 175 921
Size: 175 746
BglII A'GATC_T
Cuts at: 0 719 921
Size: 719 202
Bme1580I G_kGCm'C
Cuts at: 0 7 921
Size: 7 914
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 921
Size: 666 255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 921
Size: 649 272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 921
Size: 718 203
BsaHI Gr'CG_yC
Cuts at: 0 412 436 921
Size: 412 24 485
Fragments arranged by size:
485 412 24
BsaWI w'CCGG_w
Cuts at: 0 32 921
Size: 32 889
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 706 736 921
Size: 706 30 185
Fragments arranged by size:
706 185 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 354 357 627 892 921
Size: 354 3 270 265 29
Fragments arranged by size:
354 270 265 29 3
BseYI C'CCAG_C
Cuts at: 0 25 296 311 921
Size: 25 271 15 610
Fragments arranged by size:
610 271 25 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 305 921
Size: 223 82 616
Fragments arranged by size:
616 223 82
BsiEI CG_ry'CG
Cuts at: 0 86 921
Size: 86 835
BsiHKAI G_wGCw'C
Cuts at: 0 527 665 808 921
Size: 527 138 143 113
Fragments arranged by size:
527 143 138 113
Bsp1286I G_dGCh'C
Cuts at: 0 7 364 527 652 665 808 921
Size: 7 357 163 125 13 143 113
Fragments arranged by size:
357 163 143 125 113 13 7
BspHI T'CATG_A
Cuts at: 0 800 921
Size: 800 121
BsrBI CCG'CTC
Cuts at: 0 548 732 878 921
Size: 548 184 146 43
Fragments arranged by size:
548 184 146 43
BsrFI r'CCGG_y
Cuts at: 0 306 482 591 921
Size: 306 176 109 330
Fragments arranged by size:
330 306 176 109
BssSI C'ACGA_G
Cuts at: 0 658 921
Size: 658 263
BstYI r'GATC_y
Cuts at: 0 719 921
Size: 719 202
BtgI C'CryG_G
Cuts at: 0 68 512 921
Size: 68 444 409
Fragments arranged by size:
444 409 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 30 65 921
Size: 30 35 856
Fragments arranged by size:
856 35 30
EaeI y'GGCC_r
Cuts at: 0 62 83 167 176 329 593 879 921
Size: 62 21 84 9 153 264 286 42
Fragments arranged by size:
286 264 153 84 62 42 21 9
EagI C'GGCC_G
Cuts at: 0 83 921
Size: 83 838
EarI CTCTTCn'nnn_
Cuts at: 0 151 921
Size: 151 770
EcoICRI GAG'CTC
Cuts at: 0 663 806 921
Size: 663 143 115
Fragments arranged by size:
663 143 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 277 517 649 886 921
Size: 142 135 240 132 237 35
Fragments arranged by size:
240 237 142 135 132 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 921
Size: 783 138
FspI TGC'GCA
Cuts at: 0 535 921
Size: 535 386
HaeII r_GCGC'y
Cuts at: 0 129 921
Size: 129 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 921
Size: 619 32 270
Fragments arranged by size:
619 270 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 921
Size: 884 37
MslI CAynn'nnrTG
Cuts at: 0 142 921
Size: 142 779
NaeI GCC'GGC
Cuts at: 0 593 921
Size: 593 328
NgoMIV G'CCGG_C
Cuts at: 0 591 921
Size: 591 330
NspI r_CATG'y
Cuts at: 0 618 686 921
Size: 618 68 235
Fragments arranged by size:
618 235 68
PstI C_TGCA'G
Cuts at: 0 563 625 821 921
Size: 563 62 196 100
Fragments arranged by size:
563 196 100 62
SacI G_AGCT'C
Cuts at: 0 665 808 921
Size: 665 143 113
Fragments arranged by size:
665 143 113
SfcI C'TryA_G
Cuts at: 0 559 621 817 921
Size: 559 62 196 104
Fragments arranged by size:
559 196 104 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 175 921
Size: 175 746
SgrAI Cr'CCGG_yG
Cuts at: 0 482 921
Size: 482 439
SmlI C'TyrA_G
Cuts at: 0 733 921
Size: 733 188
StuI AGG'CCT
Cuts at: 0 766 911 921
Size: 766 145 10
Fragments arranged by size:
766 145 10
StyI C'CwwG_G
Cuts at: 0 107 151 906 921
Size: 107 44 755 15
Fragments arranged by size:
755 107 44 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 921
Size: 454 467
Enzymes that do cut:
AcuI AlfI AvaI AvrII BanII BbsI BglI BglII
Bme1580I BplI BpmI BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrFI
BssSI BstYI BtgI CspCI EaeI EagI EarI EcoICRI
Eco57MI FalI FspI HaeII Hin4I MmeI MslI NaeI
NgoMIV NspI PstI SacI SfcI SfiI SgrAI SmlI
StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI
Bpu10I BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspEI
BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI
EciI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI