Restriction map for
pF1KB9448
(Linear) (Six-base) MAPSORT of: KIBB9448.seq Check: 3340 from: 1 to: 1461
KIBB9448.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AleI CACnn'nnGTG
Cuts at: 0 403 1461
Size: 403 1058
AlwNI CAG_nnn'CTG
Cuts at: 0 255 479 1447 1461
Size: 255 224 968 14
Fragments arranged by size:
968 255 224 14
ApoI r'AATT_y
Cuts at: 0 743 1461
Size: 743 718
AvaI C'yCGr_G
Cuts at: 0 359 914 1461
Size: 359 555 547
Fragments arranged by size:
555 547 359
BanII G_rGCy'C
Cuts at: 0 538 821 1207 1461
Size: 538 283 386 254
Fragments arranged by size:
538 386 283 254
BlpI GC'TnA_GC
Cuts at: 0 1333 1461
Size: 1333 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 1343 1461
Size: 231 1112 118
Fragments arranged by size:
1112 231 118
BmtI G_CTAG'C
Cuts at: 0 179 1461
Size: 179 1282
BsaBI GATnn'nnATC
Cuts at: 0 722 1461
Size: 722 739
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 520 532 550 994 1024 1461
Size: 502 18 12 18 444 30 437
Fragments arranged by size:
502 444 437 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 751 1188 1461
Size: 154 597 437 273
Fragments arranged by size:
597 437 273 154
BseYI C'CCAG_C
Cuts at: 0 318 522 1461
Size: 318 204 939
Fragments arranged by size:
939 318 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1461
Size: 427 1034
BsiHKAI G_wGCw'C
Cuts at: 0 512 821 1207 1217 1461
Size: 512 309 386 10 244
Fragments arranged by size:
512 386 309 244 10
BsmI GAATG_Cn'
Cuts at: 0 1286 1461
Size: 1286 175
Bsp1286I G_dGCh'C
Cuts at: 0 512 538 821 1207 1217 1461
Size: 512 26 283 386 10 244
Fragments arranged by size:
512 386 283 244 26 10
BspHI T'CATG_A
Cuts at: 0 813 1461
Size: 813 648
BsrBI CCG'CTC
Cuts at: 0 612 1461
Size: 612 849
BsrDI GCAATG_nn'
Cuts at: 0 360 1461
Size: 360 1101
BssSI C'ACGA_G
Cuts at: 0 163 1461
Size: 163 1298
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 100 1461
Size: 24 76 1361
Fragments arranged by size:
1361 76 24
BtsI GCAGTG_nn'
Cuts at: 0 451 567 625 1461
Size: 451 116 58 836
Fragments arranged by size:
836 451 116 58
DraI TTT'AAA
Cuts at: 0 374 1391 1461
Size: 374 1017 70
Fragments arranged by size:
1017 374 70
EaeI y'GGCC_r
Cuts at: 0 683 1461
Size: 683 778
EarI CTCTTCn'nnn_
Cuts at: 0 131 873 1048 1205 1461
Size: 131 742 175 157 256
Fragments arranged by size:
742 256 175 157 131
EcoICRI GAG'CTC
Cuts at: 0 819 1205 1461
Size: 819 386 256
Fragments arranged by size:
819 386 256
EcoNI CCTnn'n_nnAGG
Cuts at: 0 15 1461
Size: 15 1446
EcoO109I rG'GnC_Cy
Cuts at: 0 20 1461
Size: 20 1441
HaeII r_GCGC'y
Cuts at: 0 571 1461
Size: 571 890
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 520 552 964 996 1345 1377
Size: 209 32 279 32 412 32 349 32
Cuts at: 1377 1461
Size: 84
Fragments arranged by size:
412 349 279 209 84 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1461
Size: 117 1344
MscI TGG'CCA
Cuts at: 0 685 1461
Size: 685 776
MslI CAynn'nnrTG
Cuts at: 0 312 403 1461
Size: 312 91 1058
Fragments arranged by size:
1058 312 91
MspA1I CmG'CkG
Cuts at: 0 39 1461
Size: 39 1422
NheI G'CTAG_C
Cuts at: 0 175 1461
Size: 175 1286
NspI r_CATG'y
Cuts at: 0 1168 1461
Size: 1168 293
PasI CC'CwG_GG
Cuts at: 0 24 235 1461
Size: 24 211 1226
Fragments arranged by size:
1226 211 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 479 1461
Size: 479 982
PfoI T'CCnGG_A
Cuts at: 0 1022 1461
Size: 1022 439
PpuMI rG'GwC_Cy
Cuts at: 0 20 1461
Size: 20 1441
PstI C_TGCA'G
Cuts at: 0 206 334 458 1163 1188 1461
Size: 206 128 124 705 25 273
Fragments arranged by size:
705 273 206 128 124 25
PvuII CAG'CTG
Cuts at: 0 39 1461
Size: 39 1422
SacI G_AGCT'C
Cuts at: 0 821 1207 1461
Size: 821 386 254
Fragments arranged by size:
821 386 254
SapI GCTCTTCn'nnn_
Cuts at: 0 1205 1461
Size: 1205 256
SfcI C'TryA_G
Cuts at: 0 202 330 454 1159 1184 1461
Size: 202 128 124 705 25 277
Fragments arranged by size:
705 277 202 128 124 25
SmaI CCC'GGG
Cuts at: 0 361 1461
Size: 361 1100
SmlI C'TyrA_G
Cuts at: 0 914 1461
Size: 914 547
SphI G_CATG'C
Cuts at: 0 1168 1461
Size: 1168 293
SspI AAT'ATT
Cuts at: 0 50 1461
Size: 50 1411
StyI C'CwwG_G
Cuts at: 0 896 1380 1461
Size: 896 484 81
Fragments arranged by size:
896 484 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 741 773 1461
Size: 502 32 207 32 688
Fragments arranged by size:
688 502 207 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 223 1461
Size: 223 1238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 1461
Size: 678 783
XhoI C'TCGA_G
Cuts at: 0 914 1461
Size: 914 547
XmaI C'CCGG_G
Cuts at: 0 359 1461
Size: 359 1102
Enzymes that do cut:
AleI AlwNI ApoI AvaI BanII BlpI BmrI BmtI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BsrDI BssSI BstXI BtsI DraI EaeI
EarI EcoICRI EcoNI EcoO109I HaeII Hin4I MmeI MscI
MslI MspA1I NheI NspI PasI PflMI PfoI PpuMI
PstI PvuII SacI SapI SfcI SmaI SmlI SphI
SspI StyI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI Eco57MI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
TaqII TatI XbaI XmnI ZraI