Restriction map for
pF1KB9451
(Linear) (Six-base) MAPSORT of: KIBB9451.seq Check: 6601 from: 1 to: 996
KIBB9451.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AfeI AGC'GCT
Cuts at: 0 469 996
Size: 469 527
ApaI G_GGCC'C
Cuts at: 0 860 996
Size: 860 136
AscI GG'CGCG_CC
Cuts at: 0 863 996
Size: 863 133
AvaI C'yCGr_G
Cuts at: 0 286 955 996
Size: 286 669 41
Fragments arranged by size:
669 286 41
BanI G'GyrC_C
Cuts at: 0 4 157 555 648 996
Size: 4 153 398 93 348
Fragments arranged by size:
398 348 153 93 4
BanII G_rGCy'C
Cuts at: 0 215 347 782 860 902 996
Size: 215 132 435 78 42 94
Fragments arranged by size:
435 215 132 94 78 42
BbeI G_GCGC'C
Cuts at: 0 161 559 652 996
Size: 161 398 93 344
Fragments arranged by size:
398 344 161 93
BbvCI CC'TCA_GC
Cuts at: 0 551 996
Size: 551 445
BglI GCCn_nnn'nGGC
Cuts at: 0 289 382 616 811 838 996
Size: 289 93 234 195 27 158
Fragments arranged by size:
289 234 195 158 93 27
BlpI GC'TnA_GC
Cuts at: 0 496 996
Size: 496 500
Bme1580I G_kGCm'C
Cuts at: 0 9 860 996
Size: 9 851 136
Fragments arranged by size:
851 136 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 996
Size: 3 993
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 861 996
Size: 366 495 135
Fragments arranged by size:
495 366 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 996
Size: 554 442
Bpu10I CC'TnA_GC
Cuts at: 0 551 996
Size: 551 445
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 754 996
Size: 439 315 242
Fragments arranged by size:
439 315 242
BsaAI yAC'GTr
Cuts at: 0 114 996
Size: 114 882
BsaHI Gr'CG_yC
Cuts at: 0 158 238 556 649 996
Size: 158 80 318 93 347
Fragments arranged by size:
347 318 158 93 80
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 223 381 673 691 790 808 823
Size: 181 42 158 292 18 99 18 15
Cuts at: 823 910 996
Size: 87 86
Fragments arranged by size:
292 181 158 99 87 86 42 18
18 15
BseYI C'CCAG_C
Cuts at: 0 436 781 950 978 996
Size: 436 345 169 28 18
Fragments arranged by size:
436 345 169 28 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 996
Size: 981 15
BsiEI CG_ry'CG
Cuts at: 0 146 197 221 254 272 362 386 608
Size: 146 51 24 33 18 90 24 222
Cuts at: 608 908 996
Size: 300 88
Fragments arranged by size:
300 222 146 90 88 51 33 24
24 18
Bsp1286I G_dGCh'C
Cuts at: 0 9 215 347 782 860 902 996
Size: 9 206 132 435 78 42 94
Fragments arranged by size:
435 206 132 94 78 42 9
BsrBI CCG'CTC
Cuts at: 0 230 500 996
Size: 230 270 496
Fragments arranged by size:
496 270 230
BsrFI r'CCGG_y
Cuts at: 0 267 996
Size: 267 729
BssHII G'CGCG_C
Cuts at: 0 863 996
Size: 863 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 996
Size: 789 207
BtgI C'CryG_G
Cuts at: 0 44 140 215 248 323 356 362 890
Size: 44 96 75 33 75 33 6 528
Cuts at: 890 902 996
Size: 12 94
Fragments arranged by size:
528 96 94 75 75 44 33 33
12 6
EaeI y'GGCC_r
Cuts at: 0 108 143 194 218 251 269 359 383
Size: 108 35 51 24 33 18 90 24
Cuts at: 383 605 905 996
Size: 222 300 91
Fragments arranged by size:
300 222 108 91 90 51 35 33
24 24 18
EagI C'GGCC_G
Cuts at: 0 143 194 218 251 269 359 383 605
Size: 143 51 24 33 18 90 24 222
Cuts at: 605 905 996
Size: 300 91
Fragments arranged by size:
300 222 143 91 90 51 33 24
24 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 837 996
Size: 837 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 996
Size: 554 442
EcoO109I rG'GnC_Cy
Cuts at: 0 318 840 996
Size: 318 522 156
Fragments arranged by size:
522 318 156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 486 996
Size: 486 510
FspI TGC'GCA
Cuts at: 0 930 996
Size: 930 66
HaeII r_GCGC'y
Cuts at: 0 161 191 471 559 652 827 996
Size: 161 30 280 88 93 175 169
Fragments arranged by size:
280 175 169 161 93 88 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 996
Size: 364 32 600
Fragments arranged by size:
600 364 32
KasI G'GCGC_C
Cuts at: 0 157 555 648 996
Size: 157 398 93 348
Fragments arranged by size:
398 348 157 93
MscI TGG'CCA
Cuts at: 0 110 996
Size: 110 886
MspA1I CmG'CkG
Cuts at: 0 46 142 217 250 280 325 364 562
Size: 46 96 75 33 30 45 39 198
Cuts at: 562 601 646 670 727 892 904 982 996
Size: 39 45 24 57 165 12 78 14
Fragments arranged by size:
198 165 96 78 75 57 46 45
45 39 39 33 30 24 14 12
NaeI GCC'GGC
Cuts at: 0 269 996
Size: 269 727
NarI GG'CG_CC
Cuts at: 0 158 556 649 996
Size: 158 398 93 347
Fragments arranged by size:
398 347 158 93
NgoMIV G'CCGG_C
Cuts at: 0 267 996
Size: 267 729
NotI GC'GGCC_GC
Cuts at: 0 143 218 605 905 996
Size: 143 75 387 300 91
Fragments arranged by size:
387 300 143 91 75
PmlI CAC'GTG
Cuts at: 0 114 996
Size: 114 882
PspOMI G'GGCC_C
Cuts at: 0 856 996
Size: 856 140
PstI C_TGCA'G
Cuts at: 0 176 996
Size: 176 820
PvuII CAG'CTG
Cuts at: 0 601 996
Size: 601 395
SacII CC_GC'GG
Cuts at: 0 47 143 218 251 326 365 893 905
Size: 47 96 75 33 75 39 528 12
Cuts at: 905 996
Size: 91
Fragments arranged by size:
528 96 91 75 75 47 39 33
12
SfcI C'TryA_G
Cuts at: 0 172 996
Size: 172 824
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 382 811 996
Size: 382 429 185
Fragments arranged by size:
429 382 185
SfoI GGC'GCC
Cuts at: 0 159 557 650 996
Size: 159 398 93 346
Fragments arranged by size:
398 346 159 93
StuI AGG'CCT
Cuts at: 0 54 996
Size: 54 942
StyI C'CwwG_G
Cuts at: 0 161 407 764 996
Size: 161 246 357 232
Fragments arranged by size:
357 246 232 161
XmnI GAAnn'nnTTC
Cuts at: 0 487 996
Size: 487 509
Enzymes that do cut:
AfeI ApaI AscI AvaI BanI BanII BbeI BbvCI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI
BsrFI BssHII BstXI BtgI EaeI EagI EciI Eco57MI
EcoO109I FalI FspI HaeII Hin4I KasI MscI MspA1I
NaeI NarI NgoMIV NotI PmlI PspOMI PstI PvuII
SacII SfcI SfiI SfoI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI BsaWI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI