Restriction map for
pF1KB9453
(Linear) (Six-base) MAPSORT of: KIBB9453.seq Check: 367 from: 1 to: 1917
KIBB9453.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 384 1917
Size: 384 1533
AccI GT'mk_AC
Cuts at: 0 1078 1917
Size: 1078 839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 697 851 1103 1917
Size: 511 186 154 252 814
Fragments arranged by size:
814 511 252 186 154
AfeI AGC'GCT
Cuts at: 0 1605 1917
Size: 1605 312
AflII C'TTAA_G
Cuts at: 0 783 1917
Size: 783 1134
AflIII A'CryG_T
Cuts at: 0 400 402 1917
Size: 400 2 1515
Fragments arranged by size:
1515 400 2
AleI CACnn'nnGTG
Cuts at: 0 829 1696 1917
Size: 829 867 221
Fragments arranged by size:
867 829 221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1664 1917
Size: 1664 253
AlwNI CAG_nnn'CTG
Cuts at: 0 982 1350 1917
Size: 982 368 567
Fragments arranged by size:
982 567 368
ApaI G_GGCC'C
Cuts at: 0 476 1169 1306 1743 1917
Size: 476 693 137 437 174
Fragments arranged by size:
693 476 437 174 137
ApoI r'AATT_y
Cuts at: 0 877 1107 1917
Size: 877 230 810
Fragments arranged by size:
877 810 230
AvaI C'yCGr_G
Cuts at: 0 134 287 1711 1917
Size: 134 153 1424 206
Fragments arranged by size:
1424 206 153 134
BanI G'GyrC_C
Cuts at: 0 1319 1698 1917
Size: 1319 379 219
Fragments arranged by size:
1319 379 219
BanII G_rGCy'C
Cuts at: 0 254 476 802 991 1169 1306 1372 1671
Size: 254 222 326 189 178 137 66 299
Cuts at: 1671 1743 1917
Size: 72 174
Fragments arranged by size:
326 299 254 222 189 178 174 137
72 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 182 773 1917
Size: 94 88 591 1144
Fragments arranged by size:
1144 591 94 88
BfrBI ATG'CAT
Cuts at: 0 1146 1917
Size: 1146 771
Bme1580I G_kGCm'C
Cuts at: 0 476 1169 1306 1322 1703 1743 1917
Size: 476 693 137 16 381 40 174
Fragments arranged by size:
693 476 381 174 137 40 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 967 1448 1917
Size: 967 481 469
Fragments arranged by size:
967 481 469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 680 1646 1871 1917
Size: 377 303 966 225 46
Fragments arranged by size:
966 377 303 225 46
Bpu10I CC'TnA_GC
Cuts at: 0 248 908 1917
Size: 248 660 1009
Fragments arranged by size:
1009 660 248
BsaI GGTCTCn'nnnn_
Cuts at: 0 197 799 1917
Size: 197 602 1118
Fragments arranged by size:
1118 602 197
BsaAI yAC'GTr
Cuts at: 0 403 1917
Size: 403 1514
BsaBI GATnn'nnATC
Cuts at: 0 526 1917
Size: 526 1391
BsaWI w'CCGG_w
Cuts at: 0 7 360 1917
Size: 7 353 1557
Fragments arranged by size:
1557 353 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 334 1322 1793 1796 1917
Size: 69 265 988 471 3 121
Fragments arranged by size:
988 471 265 121 69 3
BseYI C'CCAG_C
Cuts at: 0 975 1204 1537 1917
Size: 975 229 333 380
Fragments arranged by size:
975 380 333 229
BsiEI CG_ry'CG
Cuts at: 0 8 1917
Size: 8 1909
BsiHKAI G_wGCw'C
Cuts at: 0 802 1917
Size: 802 1115
BsmI GAATG_Cn'
Cuts at: 0 110 1917
Size: 110 1807
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1073 1917
Size: 1073 844
Bsp1286I G_dGCh'C
Cuts at: 0 254 476 802 991 1169 1306 1322 1372
Size: 254 222 326 189 178 137 16 50
Cuts at: 1372 1671 1703 1743 1917
Size: 299 32 40 174
Fragments arranged by size:
326 299 254 222 189 178 174 137
50 40 32 16
BspEI T'CCGG_A
Cuts at: 0 360 1917
Size: 360 1557
BspHI T'CATG_A
Cuts at: 0 419 1917
Size: 419 1498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 384 1917
Size: 384 1533
BsrBI CCG'CTC
Cuts at: 0 324 1917
Size: 324 1593
BsrDI GCAATG_nn'
Cuts at: 0 519 860 1917
Size: 519 341 1057
Fragments arranged by size:
1057 519 341
BsrFI r'CCGG_y
Cuts at: 0 1777 1917
Size: 1777 140
BssSI C'ACGA_G
Cuts at: 0 1599 1917
Size: 1599 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 633 889 1017 1268 1315 1917
Size: 27 606 256 128 251 47 602
Fragments arranged by size:
606 602 256 251 128 47 27
BstYI r'GATC_y
Cuts at: 0 779 1917
Size: 779 1138
Bsu36I CC'TnA_GG
Cuts at: 0 227 1917
Size: 227 1690
BtgI C'CryG_G
Cuts at: 0 1137 1323 1495 1570 1627 1817 1917
Size: 1137 186 172 75 57 190 100
Fragments arranged by size:
1137 190 186 172 100 75 57
BtsI GCAGTG_nn'
Cuts at: 0 659 1917
Size: 659 1258
DraIII CAC_nnn'GTG
Cuts at: 0 833 1917
Size: 833 1084
EaeI y'GGCC_r
Cuts at: 0 576 855 1019 1917
Size: 576 279 164 898
Fragments arranged by size:
898 576 279 164
EarI CTCTTCn'nnn_
Cuts at: 0 46 311 1493 1917
Size: 46 265 1182 424
Fragments arranged by size:
1182 424 265 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1511 1523 1742 1764 1799 1917
Size: 1511 12 219 22 35 118
Fragments arranged by size:
1511 219 118 35 22 12
EcoICRI GAG'CTC
Cuts at: 0 800 1917
Size: 800 1117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 511 680 697 851 1103 1646 1871
Size: 377 134 169 17 154 252 543 225
Cuts at: 1871 1917
Size: 46
Fragments arranged by size:
543 377 252 225 169 154 134 46
17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 1917
Size: 679 1238
EcoO109I rG'GnC_Cy
Cuts at: 0 236 755 1302 1303 1739 1740 1917
Size: 236 519 547 1 436 1 177
Fragments arranged by size:
547 519 436 236 177 1 1
EcoRI G'AATT_C
Cuts at: 0 1107 1917
Size: 1107 810
FspI TGC'GCA
Cuts at: 0 196 1917
Size: 196 1721
HaeII r_GCGC'y
Cuts at: 0 1607 1917
Size: 1607 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 886 918 1917
Size: 220 32 634 32 999
Fragments arranged by size:
999 634 220 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1917
Size: 8 1909
MscI TGG'CCA
Cuts at: 0 578 857 1021 1917
Size: 578 279 164 896
Fragments arranged by size:
896 578 279 164
MslI CAynn'nnrTG
Cuts at: 0 343 829 1151 1696 1843 1855 1917
Size: 343 486 322 545 147 12 62
Fragments arranged by size:
545 486 343 322 147 62 12
MspA1I CmG'CkG
Cuts at: 0 62 1917
Size: 62 1855
NaeI GCC'GGC
Cuts at: 0 1779 1917
Size: 1779 138
NcoI C'CATG_G
Cuts at: 0 1323 1495 1570 1627 1817 1917
Size: 1323 172 75 57 190 100
Fragments arranged by size:
1323 190 172 100 75 57
NgoMIV G'CCGG_C
Cuts at: 0 1777 1917
Size: 1777 140
NsiI A_TGCA'T
Cuts at: 0 1148 1917
Size: 1148 769
NspI r_CATG'y
Cuts at: 0 1146 1869 1917
Size: 1146 723 48
Fragments arranged by size:
1146 723 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 1768 1917
Size: 1768 149
PfoI T'CCnGG_A
Cuts at: 0 99 1662 1917
Size: 99 1563 255
Fragments arranged by size:
1563 255 99
PmlI CAC'GTG
Cuts at: 0 403 1917
Size: 403 1514
PpuMI rG'GwC_Cy
Cuts at: 0 236 755 1917
Size: 236 519 1162
Fragments arranged by size:
1162 519 236
PspOMI G'GGCC_C
Cuts at: 0 472 1165 1302 1739 1917
Size: 472 693 137 437 178
Fragments arranged by size:
693 472 437 178 137
PstI C_TGCA'G
Cuts at: 0 170 381 1917
Size: 170 211 1536
Fragments arranged by size:
1536 211 170
SacI G_AGCT'C
Cuts at: 0 802 1917
Size: 802 1115
SanDI GG'GwC_CC
Cuts at: 0 755 1917
Size: 755 1162
SfcI C'TryA_G
Cuts at: 0 110 166 377 1917
Size: 110 56 211 1540
Fragments arranged by size:
1540 211 110 56
SmaI CCC'GGG
Cuts at: 0 1713 1917
Size: 1713 204
SmlI C'TyrA_G
Cuts at: 0 134 783 1917
Size: 134 649 1134
Fragments arranged by size:
1134 649 134
SphI G_CATG'C
Cuts at: 0 1146 1869 1917
Size: 1146 723 48
Fragments arranged by size:
1146 723 48
SspI AAT'ATT
Cuts at: 0 937 1917
Size: 937 980
StuI AGG'CCT
Cuts at: 0 888 1917
Size: 888 1029
StyI C'CwwG_G
Cuts at: 0 1267 1323 1495 1570 1627 1817 1917
Size: 1267 56 172 75 57 190 100
Fragments arranged by size:
1267 190 172 100 75 57 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 949 1099 1211 1917
Size: 949 150 112 706
Fragments arranged by size:
949 706 150 112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1014 1144 1545 1848 1917
Size: 1014 130 401 303 69
Fragments arranged by size:
1014 401 303 130 69
XhoI C'TCGA_G
Cuts at: 0 134 1917
Size: 134 1783
XmaI C'CCGG_G
Cuts at: 0 1711 1917
Size: 1711 206
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlfI
AlwNI ApaI ApoI AvaI BanI BanII BbsI BfrBI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PflMI PfoI PmlI PpuMI PspOMI PstI SacI SanDI
SfcI SmaI SmlI SphI SspI StuI StyI TaqII
XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaHI BsaXI BsgI BsiWI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI PacI PasI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SwaI TatI TstI Tth111I XbaI XmnI
ZraI