Restriction map for
pF1KB9457
(Linear) (Six-base) MAPSORT of: KIBB9457.seq Check: 9256 from: 1 to: 2496
KIBB9457.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AatII G_ACGT'C
Cuts at: 0 256 2496
Size: 256 2240
AccI GT'mk_AC
Cuts at: 0 524 2440 2496
Size: 524 1916 56
Fragments arranged by size:
1916 524 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 638 2496
Size: 286 352 1858
Fragments arranged by size:
1858 352 286
AflIII A'CryG_T
Cuts at: 0 1239 1426 1711 2496
Size: 1239 187 285 785
Fragments arranged by size:
1239 785 285 187
AhdI GACnn_n'nnGTC
Cuts at: 0 547 2496
Size: 547 1949
AleI CACnn'nnGTG
Cuts at: 0 1529 2496
Size: 1529 967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1547 1903 2496
Size: 1547 356 593
Fragments arranged by size:
1547 593 356
AlwNI CAG_nnn'CTG
Cuts at: 0 49 2496
Size: 49 2447
ApaI G_GGCC'C
Cuts at: 0 838 2496
Size: 838 1658
ApoI r'AATT_y
Cuts at: 0 34 55 78 1304 2496
Size: 34 21 23 1226 1192
Fragments arranged by size:
1226 1192 34 23 21
AvaI C'yCGr_G
Cuts at: 0 986 1610 2496
Size: 986 624 886
Fragments arranged by size:
986 886 624
BamHI G'GATC_C
Cuts at: 0 960 2496
Size: 960 1536
BanI G'GyrC_C
Cuts at: 0 9 1211 2496
Size: 9 1202 1285
Fragments arranged by size:
1285 1202 9
BanII G_rGCy'C
Cuts at: 0 409 838 2496
Size: 409 429 1658
Fragments arranged by size:
1658 429 409
BbeI G_GCGC'C
Cuts at: 0 13 2496
Size: 13 2483
BbsI GAAGACnn'nnnn_
Cuts at: 0 908 2496
Size: 908 1588
BbvCI CC'TCA_GC
Cuts at: 0 1733 2496
Size: 1733 763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1438 1472 2496
Size: 1438 34 1024
Fragments arranged by size:
1438 1024 34
BfrBI ATG'CAT
Cuts at: 0 1844 2496
Size: 1844 652
BglI GCCn_nnn'nGGC
Cuts at: 0 1787 2496
Size: 1787 709
BlpI GC'TnA_GC
Cuts at: 0 374 1535 2496
Size: 374 1161 961
Fragments arranged by size:
1161 961 374
Bme1580I G_kGCm'C
Cuts at: 0 838 1216 2496
Size: 838 378 1280
Fragments arranged by size:
1280 838 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 1102 2442 2496
Size: 1102 1340 54
Fragments arranged by size:
1340 1102 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 399 681 2496
Size: 399 282 1815
Fragments arranged by size:
1815 399 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 2255 2496
Size: 1160 1095 241
Fragments arranged by size:
1160 1095 241
Bpu10I CC'TnA_GC
Cuts at: 0 1733 2496
Size: 1733 763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1169 2496
Size: 950 219 1327
Fragments arranged by size:
1327 950 219
BsaI GGTCTCn'nnnn_
Cuts at: 0 1178 2496
Size: 1178 1318
BsaHI Gr'CG_yC
Cuts at: 0 10 253 2437 2496
Size: 10 243 2184 59
Fragments arranged by size:
2184 243 59 10
BsaWI w'CCGG_w
Cuts at: 0 597 2496
Size: 597 1899
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2113 2143 2496
Size: 2113 30 353
Fragments arranged by size:
2113 353 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1787 2380 2496
Size: 1787 593 116
Fragments arranged by size:
1787 593 116
BseYI C'CCAG_C
Cuts at: 0 1763 2496
Size: 1763 733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1220 2465 2496
Size: 500 720 1245 31
Fragments arranged by size:
1245 720 500 31
BsiHKAI G_wGCw'C
Cuts at: 0 409 2126 2496
Size: 409 1717 370
Fragments arranged by size:
1717 409 370
Bsp1286I G_dGCh'C
Cuts at: 0 409 838 1216 2126 2496
Size: 409 429 378 910 370
Fragments arranged by size:
910 429 409 378 370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 165 2248 2320 2487 2496
Size: 165 2083 72 167 9
Fragments arranged by size:
2083 167 165 72 9
BsrDI GCAATG_nn'
Cuts at: 0 1753 2496
Size: 1753 743
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1079 2496
Size: 1079 1417
BstEII G'GTnAC_C
Cuts at: 0 1702 2496
Size: 1702 794
BstXI CCAn_nnnn'nTGG
Cuts at: 0 630 2496
Size: 630 1866
BstYI r'GATC_y
Cuts at: 0 483 960 2496
Size: 483 477 1536
Fragments arranged by size:
1536 483 477
BstZ17I GTA'TAC
Cuts at: 0 525 2496
Size: 525 1971
Bsu36I CC'TnA_GG
Cuts at: 0 65 2496
Size: 65 2431
BtgI C'CryG_G
Cuts at: 0 294 1545 1753 2496
Size: 294 1251 208 743
Fragments arranged by size:
1251 743 294 208
DraI TTT'AAA
Cuts at: 0 33 1673 2496
Size: 33 1640 823
Fragments arranged by size:
1640 823 33
EaeI y'GGCC_r
Cuts at: 0 1194 1347 1788 2496
Size: 1194 153 441 708
Fragments arranged by size:
1194 708 441 153
EarI CTCTTCn'nnn_
Cuts at: 0 657 2496
Size: 657 1839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2496
Size: 19 2477
EcoICRI GAG'CTC
Cuts at: 0 407 2496
Size: 407 2089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 638 1160 2255 2496
Size: 286 352 522 1095 241
Fragments arranged by size:
1095 522 352 286 241
EcoNI CCTnn'n_nnAGG
Cuts at: 0 20 2496
Size: 20 2476
EcoO109I rG'GnC_Cy
Cuts at: 0 177 835 1390 2291 2496
Size: 177 658 555 901 205
Fragments arranged by size:
901 658 555 205 177
EcoRI G'AATT_C
Cuts at: 0 55 2496
Size: 55 2441
HaeII r_GCGC'y
Cuts at: 0 13 2496
Size: 13 2483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 533 565 945 977 2253 2285
Size: 178 32 323 32 380 32 1276 32
Cuts at: 2285 2496
Size: 211
Fragments arranged by size:
1276 380 323 211 178 32 32 32
32
HincII GTy'rAC
Cuts at: 0 930 2496
Size: 930 1566
KasI G'GCGC_C
Cuts at: 0 9 2496
Size: 9 2487
MfeI C'AATT_G
Cuts at: 0 1807 2496
Size: 1807 689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1813 2496
Size: 1186 627 683
Fragments arranged by size:
1186 683 627
MscI TGG'CCA
Cuts at: 0 1196 1349 1790 2496
Size: 1196 153 441 706
Fragments arranged by size:
1196 706 441 153
MslI CAynn'nnrTG
Cuts at: 0 1316 1431 1529 1919 2046 2174 2249 2330
Size: 1316 115 98 390 127 128 75 81
Cuts at: 2330 2487 2496
Size: 157 9
Fragments arranged by size:
1316 390 157 128 127 115 98 81
75 9
MspA1I CmG'CkG
Cuts at: 0 394 1667 1767 2496
Size: 394 1273 100 729
Fragments arranged by size:
1273 729 394 100
NarI GG'CG_CC
Cuts at: 0 10 2496
Size: 10 2486
NcoI C'CATG_G
Cuts at: 0 294 1545 2496
Size: 294 1251 951
Fragments arranged by size:
1251 951 294
NsiI A_TGCA'T
Cuts at: 0 1846 2496
Size: 1846 650
NspI r_CATG'y
Cuts at: 0 1243 1430 1715 1844 2213 2492 2496
Size: 1243 187 285 129 369 279 4
Fragments arranged by size:
1243 369 285 279 187 129 4
PasI CC'CwG_GG
Cuts at: 0 488 740 2496
Size: 488 252 1756
Fragments arranged by size:
1756 488 252
PciI A'CATG_T
Cuts at: 0 1239 1426 1711 2496
Size: 1239 187 285 785
Fragments arranged by size:
1239 785 285 187
PfoI T'CCnGG_A
Cuts at: 0 919 1386 2496
Size: 919 467 1110
Fragments arranged by size:
1110 919 467
PpuMI rG'GwC_Cy
Cuts at: 0 177 1390 2496
Size: 177 1213 1106
Fragments arranged by size:
1213 1106 177
PspOMI G'GGCC_C
Cuts at: 0 834 2496
Size: 834 1662
PstI C_TGCA'G
Cuts at: 0 1984 2496
Size: 1984 512
PvuII CAG'CTG
Cuts at: 0 394 1667 1767 2496
Size: 394 1273 100 729
Fragments arranged by size:
1273 729 394 100
SacI G_AGCT'C
Cuts at: 0 409 2496
Size: 409 2087
SexAI A'CCwGG_T
Cuts at: 0 1583 2496
Size: 1583 913
SfcI C'TryA_G
Cuts at: 0 1980 2496
Size: 1980 516
SfoI GGC'GCC
Cuts at: 0 11 2496
Size: 11 2485
SmlI C'TyrA_G
Cuts at: 0 965 1184 2496
Size: 965 219 1312
Fragments arranged by size:
1312 965 219
SspI AAT'ATT
Cuts at: 0 1506 2496
Size: 1506 990
StuI AGG'CCT
Cuts at: 0 735 2496
Size: 735 1761
StyI C'CwwG_G
Cuts at: 0 294 313 1545 2496
Size: 294 19 1232 951
Fragments arranged by size:
1232 951 294 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 466 2496
Size: 466 2030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2113 2145 2496
Size: 2113 32 351
Fragments arranged by size:
2113 351 32
XbaI T'CTAG_A
Cuts at: 0 667 2496
Size: 667 1829
XmnI GAAnn'nnTTC
Cuts at: 0 59 1091 2496
Size: 59 1032 1405
Fragments arranged by size:
1405 1032 59
ZraI GAC'GTC
Cuts at: 0 254 2496
Size: 254 2242
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BfrBI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PciI PfoI PpuMI PspOMI
PstI PvuII SacI SexAI SfcI SfoI SmlI SspI
StuI StyI TaqII TstI XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BciVI BclI BglII
BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI Tth111I XcmI XhoI XmaI