Restriction map for
pF1KB9458
(Linear) (Six-base) MAPSORT of: KIBB9458.seq Check: 3640 from: 1 to: 2511
KIBB9458.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1171 1903 2166 2511
Size: 1171 732 263 345
Fragments arranged by size:
1171 732 345 263
AatII G_ACGT'C
Cuts at: 0 677 2511
Size: 677 1834
AccI GT'mk_AC
Cuts at: 0 1316 1451 1742 2511
Size: 1316 135 291 769
Fragments arranged by size:
1316 769 291 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 985 1569 2205 2479 2511
Size: 574 411 584 636 274 32
Fragments arranged by size:
636 584 574 411 274 32
AfeI AGC'GCT
Cuts at: 0 386 2511
Size: 386 2125
AflII C'TTAA_G
Cuts at: 0 2134 2511
Size: 2134 377
AgeI A'CCGG_T
Cuts at: 0 198 2210 2511
Size: 198 2012 301
Fragments arranged by size:
2012 301 198
AleI CACnn'nnGTG
Cuts at: 0 1573 1774 2260 2511
Size: 1573 201 486 251
Fragments arranged by size:
1573 486 251 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 871 2188 2511
Size: 871 1317 323
Fragments arranged by size:
1317 871 323
AlwNI CAG_nnn'CTG
Cuts at: 0 311 448 1554 2354 2511
Size: 311 137 1106 800 157
Fragments arranged by size:
1106 800 311 157 137
ApaI G_GGCC'C
Cuts at: 0 405 1124 2511
Size: 405 719 1387
Fragments arranged by size:
1387 719 405
ApaLI G'TGCA_C
Cuts at: 0 765 1126 2511
Size: 765 361 1385
Fragments arranged by size:
1385 765 361
AvaI C'yCGr_G
Cuts at: 0 393 1075 2283 2399 2422 2511
Size: 393 682 1208 116 23 89
Fragments arranged by size:
1208 682 393 116 89 23
BanI G'GyrC_C
Cuts at: 0 201 1085 2511
Size: 201 884 1426
Fragments arranged by size:
1426 884 201
BanII G_rGCy'C
Cuts at: 0 394 405 980 1076 1124 2511
Size: 394 11 575 96 48 1387
Fragments arranged by size:
1387 575 394 96 48 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 252 466 1510 1743 1799 1962 2285 2472
Size: 252 214 1044 233 56 163 323 187
Cuts at: 2472 2511
Size: 39
Fragments arranged by size:
1044 323 252 233 214 187 163 56
39
BbvCI CC'TCA_GC
Cuts at: 0 2474 2511
Size: 2474 37
BclI T'GATC_A
Cuts at: 0 1331 2511
Size: 1331 1180
BlpI GC'TnA_GC
Cuts at: 0 2417 2511
Size: 2417 94
Bme1580I G_kGCm'C
Cuts at: 0 405 769 1124 1130 2511
Size: 405 364 355 6 1381
Fragments arranged by size:
1381 405 364 355 6
BmgBI CAC'GTC
Cuts at: 0 1829 2511
Size: 1829 682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 152 1191 2511
Size: 152 1039 1320
Fragments arranged by size:
1320 1039 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 333 547 991 1039 1204 1421 1442
Size: 10 323 214 444 48 165 217 21
Cuts at: 1442 1921 1957 2511
Size: 479 36 554
Fragments arranged by size:
554 479 444 323 217 214 165 48
36 21 10
Bpu10I CC'TnA_GC
Cuts at: 0 2160 2314 2474 2511
Size: 2160 154 160 37
Fragments arranged by size:
2160 160 154 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 2511
Size: 1579 932
BsaI GGTCTCn'nnnn_
Cuts at: 0 1204 1712 2459 2511
Size: 1204 508 747 52
Fragments arranged by size:
1204 747 508 52
BsaBI GATnn'nnATC
Cuts at: 0 934 1681 2511
Size: 934 747 830
Fragments arranged by size:
934 830 747
BsaHI Gr'CG_yC
Cuts at: 0 674 1000 2300 2511
Size: 674 326 1300 211
Fragments arranged by size:
1300 674 326 211
BsaWI w'CCGG_w
Cuts at: 0 198 2210 2511
Size: 198 2012 301
Fragments arranged by size:
2012 301 198
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 659 689 1063 1093 2511
Size: 27 632 30 374 30 1418
Fragments arranged by size:
1418 632 374 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 105 988 1122 1460 2511
Size: 105 883 134 338 1051
Fragments arranged by size:
1051 883 338 134 105
BseYI C'CCAG_C
Cuts at: 0 37 381 387 405 777 1018 2511
Size: 37 344 6 18 372 241 1493
Fragments arranged by size:
1493 372 344 241 37 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 595 844 937 1101 2511
Size: 475 120 249 93 164 1410
Fragments arranged by size:
1410 475 249 164 120 93
BsiEI CG_ry'CG
Cuts at: 0 821 893 1495 2511
Size: 821 72 602 1016
Fragments arranged by size:
1016 821 602 72
BsiHKAI G_wGCw'C
Cuts at: 0 769 980 1130 2400 2511
Size: 769 211 150 1270 111
Fragments arranged by size:
1270 769 211 150 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 621 2511
Size: 181 440 1890
Fragments arranged by size:
1890 440 181
Bsp1286I G_dGCh'C
Cuts at: 0 394 405 769 980 1076 1124 1130 2400
Size: 394 11 364 211 96 48 6 1270
Cuts at: 2400 2511
Size: 111
Fragments arranged by size:
1270 394 364 211 111 96 48 11
6
BspHI T'CATG_A
Cuts at: 0 1694 2511
Size: 1694 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 497 1171 1903 2166 2511
Size: 497 674 732 263 345
Fragments arranged by size:
732 674 497 345 263
BsrBI CCG'CTC
Cuts at: 0 2159 2511
Size: 2159 352
BsrDI GCAATG_nn'
Cuts at: 0 2030 2511
Size: 2030 481
BsrFI r'CCGG_y
Cuts at: 0 198 2210 2511
Size: 198 2012 301
Fragments arranged by size:
2012 301 198
BsrGI T'GTAC_A
Cuts at: 0 920 1325 2511
Size: 920 405 1186
Fragments arranged by size:
1186 920 405
BssSI C'ACGA_G
Cuts at: 0 117 2511
Size: 117 2394
BstEII G'GTnAC_C
Cuts at: 0 645 2511
Size: 645 1866
BstXI CCAn_nnnn'nTGG
Cuts at: 0 777 1776 1899 1926 2511
Size: 777 999 123 27 585
Fragments arranged by size:
999 777 585 123 27
BstYI r'GATC_y
Cuts at: 0 308 1064 1427 2511
Size: 308 756 363 1084
Fragments arranged by size:
1084 756 363 308
Bsu36I CC'TnA_GG
Cuts at: 0 252 2511
Size: 252 2259
BtgI C'CryG_G
Cuts at: 0 46 690 1343 1364 1571 1613 1662 2511
Size: 46 644 653 21 207 42 49 849
Fragments arranged by size:
849 653 644 207 49 46 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 600 2511
Size: 600 1911
BtsI GCAGTG_nn'
Cuts at: 0 607 2511
Size: 607 1904
DraIII CAC_nnn'GTG
Cuts at: 0 1109 2511
Size: 1109 1402
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 2476 2511
Size: 1282 1194 35
Fragments arranged by size:
1282 1194 35
EaeI y'GGCC_r
Cuts at: 0 49 758 818 878 890 1492 1565 2511
Size: 49 709 60 60 12 602 73 946
Fragments arranged by size:
946 709 602 73 60 60 49 12
EagI C'GGCC_G
Cuts at: 0 818 890 1492 2511
Size: 818 72 602 1019
Fragments arranged by size:
1019 818 602 72
EarI CTCTTCn'nnn_
Cuts at: 0 333 2119 2511
Size: 333 1786 392
Fragments arranged by size:
1786 392 333
EcoICRI GAG'CTC
Cuts at: 0 978 2511
Size: 978 1533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 333 547 574 985 991 1039 1204
Size: 10 323 214 27 411 6 48 165
Cuts at: 1204 1421 1442 1569 1921 1957 2205 2479 2511
Size: 217 21 127 352 36 248 274 32
Fragments arranged by size:
411 352 323 274 248 217 214 165
127 48 36 32 27 21 10 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 452 871 1356 2511
Size: 452 419 485 1155
Fragments arranged by size:
1155 485 452 419
EcoO109I rG'GnC_Cy
Cuts at: 0 401 402 1846 1859 2511
Size: 401 1 1444 13 652
Fragments arranged by size:
1444 652 401 13 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1311 1986 2511
Size: 1311 675 525
Fragments arranged by size:
1311 675 525
HaeII r_GCGC'y
Cuts at: 0 388 833 2511
Size: 388 445 1678
Fragments arranged by size:
1678 445 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 659 691 1203 1235 1417 1449 1813
Size: 29 630 32 512 32 182 32 364
Cuts at: 1813 1845 2395 2427 2511
Size: 32 550 32 84
Fragments arranged by size:
630 550 512 364 182 84 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 0 567 2055 2511
Size: 567 1488 456
Fragments arranged by size:
1488 567 456
MscI TGG'CCA
Cuts at: 0 1567 2511
Size: 1567 944
MslI CAynn'nnrTG
Cuts at: 0 1573 1774 1924 2260 2511
Size: 1573 201 150 336 251
Fragments arranged by size:
1573 336 251 201 150
MspA1I CmG'CkG
Cuts at: 0 777 1005 1018 1059 1248 1664 1774 2511
Size: 777 228 13 41 189 416 110 737
Fragments arranged by size:
777 737 416 228 189 110 41 13
NcoI C'CATG_G
Cuts at: 0 690 1343 1613 2511
Size: 690 653 270 898
Fragments arranged by size:
898 690 653 270
NspI r_CATG'y
Cuts at: 0 921 1233 1650 2028 2511
Size: 921 312 417 378 483
Fragments arranged by size:
921 483 417 378 312
PflMI CCAn_nnn'nTGG
Cuts at: 0 311 1677 2511
Size: 311 1366 834
Fragments arranged by size:
1366 834 311
PfoI T'CCnGG_A
Cuts at: 0 524 1067 1093 2111 2511
Size: 524 543 26 1018 400
Fragments arranged by size:
1018 543 524 400 26
PspOMI G'GGCC_C
Cuts at: 0 401 1120 2511
Size: 401 719 1391
Fragments arranged by size:
1391 719 401
PstI C_TGCA'G
Cuts at: 0 337 803 1265 1487 1550 1858 2096 2177
Size: 337 466 462 222 63 308 238 81
Cuts at: 2177 2511
Size: 334
Fragments arranged by size:
466 462 337 334 308 238 222 81
63
PvuII CAG'CTG
Cuts at: 0 777 1018 2511
Size: 777 241 1493
Fragments arranged by size:
1493 777 241
SacI G_AGCT'C
Cuts at: 0 980 2511
Size: 980 1531
SacII CC_GC'GG
Cuts at: 0 1665 2511
Size: 1665 846
SapI GCTCTTCn'nnn_
Cuts at: 0 333 2119 2511
Size: 333 1786 392
Fragments arranged by size:
1786 392 333
SbfI CC_TGCA'GG
Cuts at: 0 1265 1858 2511
Size: 1265 593 653
Fragments arranged by size:
1265 653 593
SfcI C'TryA_G
Cuts at: 0 333 799 1197 1261 1356 1483 1524 1546
Size: 333 466 398 64 95 127 41 22
Cuts at: 1546 1854 2092 2173 2511
Size: 308 238 81 338
Fragments arranged by size:
466 398 338 333 308 238 127 95
81 64 41 22
SgrAI Cr'CCGG_yG
Cuts at: 0 198 2511
Size: 198 2313
SmaI CCC'GGG
Cuts at: 0 2285 2511
Size: 2285 226
SmlI C'TyrA_G
Cuts at: 0 1594 2134 2399 2511
Size: 1594 540 265 112
Fragments arranged by size:
1594 540 265 112
StyI C'CwwG_G
Cuts at: 0 690 1343 1613 1631 2511
Size: 690 653 270 18 880
Fragments arranged by size:
880 690 653 270 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2032 2511
Size: 2032 479
TatI w'GTAC_w
Cuts at: 0 920 1325 1580 1886 2511
Size: 920 405 255 306 625
Fragments arranged by size:
920 625 405 306 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 2511
Size: 1063 32 1416
Fragments arranged by size:
1416 1063 32
Tth111I GACn'n_nGTC
Cuts at: 0 225 2511
Size: 225 2286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 2109 2511
Size: 840 1269 402
Fragments arranged by size:
1269 840 402
XhoI C'TCGA_G
Cuts at: 0 2399 2511
Size: 2399 112
XmaI C'CCGG_G
Cuts at: 0 2283 2511
Size: 2283 228
XmnI GAAnn'nnTTC
Cuts at: 0 2128 2152 2511
Size: 2128 24 359
Fragments arranged by size:
2128 359 24
ZraI GAC'GTC
Cuts at: 0 675 2511
Size: 675 1836
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AgeI AleI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI
BbvCI BclI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MscI MslI MspA1I NcoI NspI PflMI PfoI PspOMI
PstI PvuII SacI SacII SapI SbfI SfcI SgrAI
SmaI SmlI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflIII AhdI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI
BglI BglII BmrI BmtI BsaAI BsiWI BsmI BspEI
BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI EciI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI