Restriction map for
pF1KB9459
(Linear) (Six-base) MAPSORT of: KIBB9459.seq Check: 9663 from: 1 to: 3672
KIBB9459.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2400 3672
Size: 2400 1272
AccI GT'mk_AC
Cuts at: 0 1733 3672
Size: 1733 1939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1339 1925 2266 2726 2995 3022 3672
Size: 1244 95 586 341 460 269 27 650
Fragments arranged by size:
1244 650 586 460 341 269 95 27
AflIII A'CryG_T
Cuts at: 0 1496 1503 3672
Size: 1496 7 2169
Fragments arranged by size:
2169 1496 7
AleI CACnn'nnGTG
Cuts at: 0 614 1714 1959 3613 3672
Size: 614 1100 245 1654 59
Fragments arranged by size:
1654 1100 614 245 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1408 3672
Size: 1408 2264
AlwNI CAG_nnn'CTG
Cuts at: 0 192 2916 3672
Size: 192 2724 756
Fragments arranged by size:
2724 756 192
ApaI G_GGCC'C
Cuts at: 0 3398 3672
Size: 3398 274
ApaLI G'TGCA_C
Cuts at: 0 120 3295 3672
Size: 120 3175 377
Fragments arranged by size:
3175 377 120
ApoI r'AATT_y
Cuts at: 0 10 1792 3061 3082 3672
Size: 10 1782 1269 21 590
Fragments arranged by size:
1782 1269 590 21 10
AvaI C'yCGr_G
Cuts at: 0 1760 2891 3391 3672
Size: 1760 1131 500 281
Fragments arranged by size:
1760 1131 500 281
BamHI G'GATC_C
Cuts at: 0 68 2240 3672
Size: 68 2172 1432
Fragments arranged by size:
2172 1432 68
BanI G'GyrC_C
Cuts at: 0 1347 2458 3377 3672
Size: 1347 1111 919 295
Fragments arranged by size:
1347 1111 919 295
BanII G_rGCy'C
Cuts at: 0 44 2663 3398 3672
Size: 44 2619 735 274
Fragments arranged by size:
2619 735 274 44
BbeI G_GCGC'C
Cuts at: 0 3381 3672
Size: 3381 291
BbsI GAAGACnn'nnnn_
Cuts at: 0 446 759 3672
Size: 446 313 2913
Fragments arranged by size:
2913 446 313
BbvCI CC'TCA_GC
Cuts at: 0 793 3672
Size: 793 2879
BciVI GTATCCnnnnn_n'
Cuts at: 0 2406 2448 3672
Size: 2406 42 1224
Fragments arranged by size:
2406 1224 42
BclI T'GATC_A
Cuts at: 0 339 1307 1808 2784 3672
Size: 339 968 501 976 888
Fragments arranged by size:
976 968 888 501 339
BfrBI ATG'CAT
Cuts at: 0 1296 3672
Size: 1296 2376
BglII A'GATC_T
Cuts at: 0 689 1364 2652 2753 3672
Size: 689 675 1288 101 919
Fragments arranged by size:
1288 919 689 675 101
BlpI GC'TnA_GC
Cuts at: 0 43 3672
Size: 43 3629
Bme1580I G_kGCm'C
Cuts at: 0 124 275 401 1352 2726 3299 3398 3672
Size: 124 151 126 951 1374 573 99 274
Fragments arranged by size:
1374 951 573 274 151 126 124 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 563 636 854 1673 3672
Size: 563 73 218 819 1999
Fragments arranged by size:
1999 819 563 218 73
BmtI G_CTAG'C
Cuts at: 0 1151 3672
Size: 1151 2521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1870 1978 2368 2677 3672
Size: 224 1646 108 390 309 995
Fragments arranged by size:
1646 995 390 309 224 108
Bpu10I CC'TnA_GC
Cuts at: 0 793 2669 3672
Size: 793 1876 1003
Fragments arranged by size:
1876 1003 793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3328 3672
Size: 3328 344
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 860 3672
Size: 10 850 2812
Fragments arranged by size:
2812 850 10
BsaHI Gr'CG_yC
Cuts at: 0 3378 3672
Size: 3378 294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 221 2498 3082 3672
Size: 221 2277 584 590
Fragments arranged by size:
2277 590 584 221
BseYI C'CCAG_C
Cuts at: 0 2018 2028 2531 2636 3149 3672
Size: 2018 10 503 105 513 523
Fragments arranged by size:
2018 523 513 503 105 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1819 2378 3672
Size: 1819 559 1294
Fragments arranged by size:
1819 1294 559
BsiEI CG_ry'CG
Cuts at: 0 615 3672
Size: 615 3057
BsiHKAI G_wGCw'C
Cuts at: 0 124 423 2490 2663 3299 3672
Size: 124 299 2067 173 636 373
Fragments arranged by size:
2067 636 373 299 173 124
BsmI GAATG_Cn'
Cuts at: 0 1296 1854 2208 3672
Size: 1296 558 354 1464
Fragments arranged by size:
1464 1296 558 354
BsmBI CGTCTCn'nnnn_
Cuts at: 0 204 3672
Size: 204 3468
Bsp1286I G_dGCh'C
Cuts at: 0 44 124 275 401 423 1352 2490 2663
Size: 44 80 151 126 22 929 1138 173
Cuts at: 2663 2726 3299 3398 3672
Size: 63 573 99 274
Fragments arranged by size:
1138 929 573 274 173 151 126 99
80 63 44 22
BspHI T'CATG_A
Cuts at: 0 306 1572 2829 3672
Size: 306 1266 1257 843
Fragments arranged by size:
1266 1257 843 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 739 2400 3293 3672
Size: 739 1661 893 379
Fragments arranged by size:
1661 893 739 379
BsrDI GCAATG_nn'
Cuts at: 0 262 2462 3672
Size: 262 2200 1210
Fragments arranged by size:
2200 1210 262
BsrGI T'GTAC_A
Cuts at: 0 1138 1178 3672
Size: 1138 40 2494
Fragments arranged by size:
2494 1138 40
BssHII G'CGCG_C
Cuts at: 0 26 3672
Size: 26 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1123 2494 3294 3672
Size: 1123 1371 800 378
Fragments arranged by size:
1371 1123 800 378
BstBI TT'CG_AA
Cuts at: 0 1683 2807 3672
Size: 1683 1124 865
Fragments arranged by size:
1683 1124 865
BstEII G'GTnAC_C
Cuts at: 0 3606 3672
Size: 3606 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 934 2001 3672
Size: 934 1067 1671
Fragments arranged by size:
1671 1067 934
BstYI r'GATC_y
Cuts at: 0 68 689 1364 2240 2652 2753 3672
Size: 68 621 675 876 412 101 919
Fragments arranged by size:
919 876 675 621 412 101 68
BstZ17I GTA'TAC
Cuts at: 0 1734 3672
Size: 1734 1938
Bsu36I CC'TnA_GG
Cuts at: 0 1239 3404 3672
Size: 1239 2165 268
Fragments arranged by size:
2165 1239 268
BtgI C'CryG_G
Cuts at: 0 1671 2292 3194 3531 3672
Size: 1671 621 902 337 141
Fragments arranged by size:
1671 902 621 337 141
BtsI GCAGTG_nn'
Cuts at: 0 590 1912 3672
Size: 590 1322 1760
Fragments arranged by size:
1760 1322 590
DraIII CAC_nnn'GTG
Cuts at: 0 1842 3562 3672
Size: 1842 1720 110
Fragments arranged by size:
1842 1720 110
EaeI y'GGCC_r
Cuts at: 0 967 1863 2190 3672
Size: 967 896 327 1482
Fragments arranged by size:
1482 967 896 327
EcoICRI GAG'CTC
Cuts at: 0 2661 3672
Size: 2661 1011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1244 1339 1870 1925 1978 2266 2368
Size: 224 1020 95 531 55 53 288 102
Cuts at: 2368 2677 2726 2995 3022 3672
Size: 309 49 269 27 650
Fragments arranged by size:
1020 650 531 309 288 269 224 102
95 55 53 49 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2047 3672
Size: 2047 1625
EcoO109I rG'GnC_Cy
Cuts at: 0 885 1598 3566 3672
Size: 885 713 1968 106
Fragments arranged by size:
1968 885 713 106
HaeII r_GCGC'y
Cuts at: 0 252 3381 3672
Size: 252 3129 291
Fragments arranged by size:
3129 291 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 108 140 405 437 1186 1218
Size: 53 32 23 32 265 32 749 32
Cuts at: 1218 1299 1331 1393 1425 3642 3672
Size: 81 32 62 32 2217 30
Fragments arranged by size:
2217 749 265 81 62 53 32 32
32 32 32 32 30 23
HincII GTy'rAC
Cuts at: 0 5 392 3672
Size: 5 387 3280
Fragments arranged by size:
3280 387 5
HindIII A'AGCT_T
Cuts at: 0 241 3004 3672
Size: 241 2763 668
Fragments arranged by size:
2763 668 241
HpaI GTT'AAC
Cuts at: 0 5 392 3672
Size: 5 387 3280
Fragments arranged by size:
3280 387 5
KasI G'GCGC_C
Cuts at: 0 3377 3672
Size: 3377 295
MfeI C'AATT_G
Cuts at: 0 2418 3672
Size: 2418 1254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2537 2770 3045 3672
Size: 2537 233 275 627
Fragments arranged by size:
2537 627 275 233
MscI TGG'CCA
Cuts at: 0 1865 2192 3672
Size: 1865 327 1480
Fragments arranged by size:
1865 1480 327
MslI CAynn'nnrTG
Cuts at: 0 352 614 932 1714 1959 3613 3672
Size: 352 262 318 782 245 1654 59
Fragments arranged by size:
1654 782 352 318 262 245 59
MspA1I CmG'CkG
Cuts at: 0 1029 2284 2695 2992 3033 3361 3672
Size: 1029 1255 411 297 41 328 311
Fragments arranged by size:
1255 1029 411 328 311 297 41
NarI GG'CG_CC
Cuts at: 0 3378 3672
Size: 3378 294
NcoI C'CATG_G
Cuts at: 0 2292 3672
Size: 2292 1380
NheI G'CTAG_C
Cuts at: 0 1147 3672
Size: 1147 2525
NsiI A_TGCA'T
Cuts at: 0 1298 3672
Size: 1298 2374
NspI r_CATG'y
Cuts at: 0 598 1500 1507 3486 3672
Size: 598 902 7 1979 186
Fragments arranged by size:
1979 902 598 186 7
PasI CC'CwG_GG
Cuts at: 0 2686 3672
Size: 2686 986
PciI A'CATG_T
Cuts at: 0 1496 1503 3672
Size: 1496 7 2169
Fragments arranged by size:
2169 1496 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 2067 3562 3672
Size: 2067 1495 110
Fragments arranged by size:
2067 1495 110
PpuMI rG'GwC_Cy
Cuts at: 0 885 3672
Size: 885 2787
PshAI GACnn'nnGTC
Cuts at: 0 2262 3672
Size: 2262 1410
PspOMI G'GGCC_C
Cuts at: 0 3394 3672
Size: 3394 278
PstI C_TGCA'G
Cuts at: 0 2051 2555 3290 3604 3665 3672
Size: 2051 504 735 314 61 7
Fragments arranged by size:
2051 735 504 314 61 7
PvuI CG_AT'CG
Cuts at: 0 615 3672
Size: 615 3057
PvuII CAG'CTG
Cuts at: 0 1029 2284 2695 2992 3033 3361 3672
Size: 1029 1255 411 297 41 328 311
Fragments arranged by size:
1255 1029 411 328 311 297 41
SacI G_AGCT'C
Cuts at: 0 2663 3672
Size: 2663 1009
SfcI C'TryA_G
Cuts at: 0 575 2047 2551 2582 2878 3286 3600 3661
Size: 575 1472 504 31 296 408 314 61
Cuts at: 3661 3672
Size: 11
Fragments arranged by size:
1472 575 504 408 314 296 61 31
11
SfoI GGC'GCC
Cuts at: 0 3379 3672
Size: 3379 293
SmaI CCC'GGG
Cuts at: 0 1762 3672
Size: 1762 1910
SmlI C'TyrA_G
Cuts at: 0 3343 3672
Size: 3343 329
SpeI A'CTAG_T
Cuts at: 0 76 1857 3672
Size: 76 1781 1815
Fragments arranged by size:
1815 1781 76
StuI AGG'CCT
Cuts at: 0 1244 3672
Size: 1244 2428
StyI C'CwwG_G
Cuts at: 0 64 2292 2732 3672
Size: 64 2228 440 940
Fragments arranged by size:
2228 940 440 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1028 2206 3289 3672
Size: 1028 1178 1083 383
Fragments arranged by size:
1178 1083 1028 383
TatI w'GTAC_w
Cuts at: 0 597 1138 1178 3176 3672
Size: 597 541 40 1998 496
Fragments arranged by size:
1998 597 541 496 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 312 344 1761 1793 3672
Size: 312 32 1417 32 1879
Fragments arranged by size:
1879 1417 312 32 32
XbaI T'CTAG_A
Cuts at: 0 370 1155 3231 3672
Size: 370 785 2076 441
Fragments arranged by size:
2076 785 441 370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 2753 3405 3672
Size: 441 2312 652 267
Fragments arranged by size:
2312 652 441 267
XmaI C'CCGG_G
Cuts at: 0 1760 3672
Size: 1760 1912
XmnI GAAnn'nnTTC
Cuts at: 0 2144 3672
Size: 2144 1528
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI DraIII EaeI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII HpaI KasI
MfeI MmeI MscI MslI MspA1I NarI NcoI NheI
NsiI NspI PasI PciI PflMI PpuMI PshAI PspOMI
PstI PvuI PvuII SacI SfcI SfoI SmaI SmlI
SpeI StuI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BcgI BglI BmgBI
BplI BsaAI BsaBI BsaWI BsaXI BsiWI BspEI BsrBI
BsrFI BssSI BtgZI ClaI CspCI DraI DrdI EagI
EarI EciI EcoRI EcoRV FalI FseI FspI FspAI
KpnI MluI NaeI NdeI NgoMIV NotI NruI PacI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SphI SrfI SspI SwaI Tth111I
XhoI ZraI