Restriction map for
pF1KB9460
(Linear) (Six-base) MAPSORT of: KIBB9460.seq Check: 2020 from: 1 to: 2184
KIBB9460.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 2086 2184
Size: 847 1239 98
Fragments arranged by size:
1239 847 98
AfeI AGC'GCT
Cuts at: 0 145 902 1522 2184
Size: 145 757 620 662
Fragments arranged by size:
757 662 620 145
AflII C'TTAA_G
Cuts at: 0 1336 2184
Size: 1336 848
AleI CACnn'nnGTG
Cuts at: 0 220 2184
Size: 220 1964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 905 2184
Size: 905 1279
AlwNI CAG_nnn'CTG
Cuts at: 0 120 684 1419 1635 2184
Size: 120 564 735 216 549
Fragments arranged by size:
735 564 549 216 120
ApaI G_GGCC'C
Cuts at: 0 10 239 277 312 591 611 735 1644
Size: 10 229 38 35 279 20 124 909
Cuts at: 1644 2161 2184
Size: 517 23
Fragments arranged by size:
909 517 279 229 124 38 35 23
20 10
AscI GG'CGCG_CC
Cuts at: 0 580 2184
Size: 580 1604
AvaI C'yCGr_G
Cuts at: 0 343 562 2154 2184
Size: 343 219 1592 30
Fragments arranged by size:
1592 343 219 30
BamHI G'GATC_C
Cuts at: 0 1559 2184
Size: 1559 625
BanI G'GyrC_C
Cuts at: 0 214 739 1048 1327 1753 1924 2184
Size: 214 525 309 279 426 171 260
Fragments arranged by size:
525 426 309 279 260 214 171
BanII G_rGCy'C
Cuts at: 0 10 239 277 312 591 611 735 824
Size: 10 229 38 35 279 20 124 89
Cuts at: 824 964 1644 2045 2161 2184
Size: 140 680 401 116 23
Fragments arranged by size:
680 401 279 229 140 124 116 89
38 35 23 20 10
BbeI G_GCGC'C
Cuts at: 0 743 1331 1757 2184
Size: 743 588 426 427
Fragments arranged by size:
743 588 427 426
BbsI GAAGACnn'nnnn_
Cuts at: 0 1131 2184
Size: 1131 1053
BclI T'GATC_A
Cuts at: 0 1130 2184
Size: 1130 1054
BglI GCCn_nnn'nGGC
Cuts at: 0 14 115 272 294 615 1955 2184
Size: 14 101 157 22 321 1340 229
Fragments arranged by size:
1340 321 229 157 101 22 14
BglII A'GATC_T
Cuts at: 0 1466 2184
Size: 1466 718
BlpI GC'TnA_GC
Cuts at: 0 1906 2184
Size: 1906 278
Bme1580I G_kGCm'C
Cuts at: 0 10 239 277 312 591 611 735 1157
Size: 10 229 38 35 279 20 124 422
Cuts at: 1157 1644 2161 2184
Size: 487 517 23
Fragments arranged by size:
517 487 422 279 229 124 38 35
23 20 10
BmgBI CAC'GTC
Cuts at: 0 87 483 2184
Size: 87 396 1701
Fragments arranged by size:
1701 396 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1002 1227 2137 2184
Size: 1002 225 910 47
Fragments arranged by size:
1002 910 225 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 2184
Size: 369 1815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 646 661 1537 1645 2184
Size: 88 558 15 876 108 539
Fragments arranged by size:
876 558 539 108 88 15
Bpu10I CC'TnA_GC
Cuts at: 0 314 475 2184
Size: 314 161 1709
Fragments arranged by size:
1709 314 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 1567 1672 2184
Size: 1475 92 105 512
Fragments arranged by size:
1475 512 105 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 1715 2184
Size: 1715 469
BsaAI yAC'GTr
Cuts at: 0 1869 2184
Size: 1869 315
BsaHI Gr'CG_yC
Cuts at: 0 740 1328 1754 2184
Size: 740 588 426 430
Fragments arranged by size:
740 588 430 426
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1954 1984 2184
Size: 1954 30 200
Fragments arranged by size:
1954 200 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1027 1252 1912 2184
Size: 1027 225 660 272
Fragments arranged by size:
1027 660 272 225
BseYI C'CCAG_C
Cuts at: 0 120 823 897 1331 1782 2184
Size: 120 703 74 434 451 402
Fragments arranged by size:
703 451 434 402 120 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 2065 2125 2184
Size: 631 1434 60 59
Fragments arranged by size:
1434 631 60 59
BsiEI CG_ry'CG
Cuts at: 0 58 575 2184
Size: 58 517 1609
Fragments arranged by size:
1609 517 58
Bsp1286I G_dGCh'C
Cuts at: 0 10 239 277 312 591 611 735 824
Size: 10 229 38 35 279 20 124 89
Cuts at: 824 964 1157 1644 2045 2161 2184
Size: 140 193 487 401 116 23
Fragments arranged by size:
487 401 279 229 193 140 124 116
89 38 35 23 20 10
BsrBI CCG'CTC
Cuts at: 0 54 552 1578 1910 2184
Size: 54 498 1026 332 274
Fragments arranged by size:
1026 498 332 274 54
BsrDI GCAATG_nn'
Cuts at: 0 1592 2184
Size: 1592 592
BsrFI r'CCGG_y
Cuts at: 0 618 930 2023 2184
Size: 618 312 1093 161
Fragments arranged by size:
1093 618 312 161
BssHII G'CGCG_C
Cuts at: 0 580 653 765 767 2148 2184
Size: 580 73 112 2 1381 36
Fragments arranged by size:
1381 580 112 73 36 2
BssSI C'ACGA_G
Cuts at: 0 553 1219 1732 1849 2184
Size: 553 666 513 117 335
Fragments arranged by size:
666 553 513 335 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1635 2145 2184
Size: 1635 510 39
Fragments arranged by size:
1635 510 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 1150 2184
Size: 945 205 1034
Fragments arranged by size:
1034 945 205
BstYI r'GATC_y
Cuts at: 0 1079 1466 1559 1601 1715 2184
Size: 1079 387 93 42 114 469
Fragments arranged by size:
1079 469 387 114 93 42
Bsu36I CC'TnA_GG
Cuts at: 0 11 1105 2184
Size: 11 1094 1079
Fragments arranged by size:
1094 1079 11
BtgI C'CryG_G
Cuts at: 0 239 268 602 611 722 1973 2184
Size: 239 29 334 9 111 1251 211
Fragments arranged by size:
1251 334 239 211 111 29 9
BtsI GCAGTG_nn'
Cuts at: 0 283 538 1328 1998 2184
Size: 283 255 790 670 186
Fragments arranged by size:
790 670 283 255 186
DraIII CAC_nnn'GTG
Cuts at: 0 2145 2184
Size: 2145 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 1297 2184
Size: 1297 887
EaeI y'GGCC_r
Cuts at: 0 55 128 572 947 2018 2184
Size: 55 73 444 375 1071 166
Fragments arranged by size:
1071 444 375 166 73 55
EagI C'GGCC_G
Cuts at: 0 55 572 2184
Size: 55 517 1612
Fragments arranged by size:
1612 517 55
EarI CTCTTCn'nnn_
Cuts at: 0 383 872 1388 1469 2184
Size: 383 489 516 81 715
Fragments arranged by size:
715 516 489 383 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 2184
Size: 34 2150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 646 661 847 1537 1645 2086 2184
Size: 88 558 15 186 690 108 441 98
Fragments arranged by size:
690 558 441 186 108 98 88 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 317 595 2184
Size: 317 278 1589
Fragments arranged by size:
1589 317 278
EcoO109I rG'GnC_Cy
Cuts at: 0 7 63 235 273 274 296 308 309
Size: 7 56 172 38 1 22 12 1
Cuts at: 309 588 607 718 732 2158 2184
Size: 279 19 111 14 1426 26
Fragments arranged by size:
1426 279 172 111 56 38 26 22
19 14 12 7 1 1
FspI TGC'GCA
Cuts at: 0 1711 2184
Size: 1711 473
HaeII r_GCGC'y
Cuts at: 0 121 147 625 743 806 844 904 1331
Size: 121 26 478 118 63 38 60 427
Cuts at: 1331 1524 1757 2184
Size: 193 233 427
Fragments arranged by size:
478 427 427 233 193 121 118 63
60 38 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 2184
Size: 412 32 1740
Fragments arranged by size:
1740 412 32
HindIII A'AGCT_T
Cuts at: 0 388 2184
Size: 388 1796
KasI G'GCGC_C
Cuts at: 0 739 1327 1753 2184
Size: 739 588 426 431
Fragments arranged by size:
739 588 431 426
MfeI C'AATT_G
Cuts at: 0 1439 2184
Size: 1439 745
MscI TGG'CCA
Cuts at: 0 949 2184
Size: 949 1235
MslI CAynn'nnrTG
Cuts at: 0 220 1148 2184
Size: 220 928 1036
Fragments arranged by size:
1036 928 220
MspA1I CmG'CkG
Cuts at: 0 604 645 724 756 1635 1707 1953 2184
Size: 604 41 79 32 879 72 246 231
Fragments arranged by size:
879 604 246 231 79 72 41 32
NaeI GCC'GGC
Cuts at: 0 620 932 2184
Size: 620 312 1252
Fragments arranged by size:
1252 620 312
NarI GG'CG_CC
Cuts at: 0 740 1328 1754 2184
Size: 740 588 426 430
Fragments arranged by size:
740 588 430 426
NgoMIV G'CCGG_C
Cuts at: 0 618 930 2184
Size: 618 312 1254
Fragments arranged by size:
1254 618 312
PasI CC'CwG_GG
Cuts at: 0 714 735 2184
Size: 714 21 1449
Fragments arranged by size:
1449 714 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 684 2184
Size: 684 1500
PfoI T'CCnGG_A
Cuts at: 0 1031 2184
Size: 1031 1153
PpuMI rG'GwC_Cy
Cuts at: 0 63 718 2184
Size: 63 655 1466
Fragments arranged by size:
1466 655 63
PspOMI G'GGCC_C
Cuts at: 0 6 235 273 308 587 607 731 1640
Size: 6 229 38 35 279 20 124 909
Cuts at: 1640 2157 2184
Size: 517 27
Fragments arranged by size:
909 517 279 229 124 38 35 27
20 6
PstI C_TGCA'G
Cuts at: 0 977 1424 2184
Size: 977 447 760
Fragments arranged by size:
977 760 447
PvuII CAG'CTG
Cuts at: 0 1635 1707 2184
Size: 1635 72 477
Fragments arranged by size:
1635 477 72
RsrII CG'GwC_CG
Cuts at: 0 726 2184
Size: 726 1458
SacII CC_GC'GG
Cuts at: 0 605 725 2184
Size: 605 120 1459
Fragments arranged by size:
1459 605 120
SanDI GG'GwC_CC
Cuts at: 0 718 2184
Size: 718 1466
SapI GCTCTTCn'nnn_
Cuts at: 0 872 1469 2184
Size: 872 597 715
Fragments arranged by size:
872 715 597
SbfI CC_TGCA'GG
Cuts at: 0 977 2184
Size: 977 1207
SfcI C'TryA_G
Cuts at: 0 973 1420 2184
Size: 973 447 764
Fragments arranged by size:
973 764 447
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 615 2184
Size: 615 1569
SfoI GGC'GCC
Cuts at: 0 741 1329 1755 2184
Size: 741 588 426 429
Fragments arranged by size:
741 588 429 426
SmaI CCC'GGG
Cuts at: 0 2156 2184
Size: 2156 28
SmlI C'TyrA_G
Cuts at: 0 1336 1490 1582 1687 2184
Size: 1336 154 92 105 497
Fragments arranged by size:
1336 497 154 105 92
StuI AGG'CCT
Cuts at: 0 323 568 1447 1648 2184
Size: 323 245 879 201 536
Fragments arranged by size:
879 536 323 245 201
StyI C'CwwG_G
Cuts at: 0 920 2184
Size: 920 1264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1759 2156 2184
Size: 1759 397 28
Fragments arranged by size:
1759 397 28
TatI w'GTAC_w
Cuts at: 0 1091 1235 1397 2184
Size: 1091 144 162 787
Fragments arranged by size:
1091 787 162 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1002 2184
Size: 970 32 1182
Fragments arranged by size:
1182 970 32
XmaI C'CCGG_G
Cuts at: 0 2154 2184
Size: 2154 30
Enzymes that do cut:
AcuI AfeI AflII AleI AlfI AlwNI ApaI AscI
AvaI BamHI BanI BanII BbeI BbsI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI Bsp1286I BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HindIII KasI MfeI MscI MslI MspA1I NaeI
NarI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI
PvuII RsrII SacII SanDI SapI SbfI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AloI ApaLI ApoI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BfrBI BmtI BsaBI BsaWI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrGI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HpaI KpnI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SacI SalI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI