Restriction map for
pF1KB9472
(Linear) (Six-base) MAPSORT of: KIBB9472.seq Check: 3128 from: 1 to: 1512
KIBB9472.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 105 252 667 1512
Size: 105 147 415 845
Fragments arranged by size:
845 415 147 105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 643 862 1512
Size: 271 372 219 650
Fragments arranged by size:
650 372 271 219
AfeI AGC'GCT
Cuts at: 0 1317 1512
Size: 1317 195
AflIII A'CryG_T
Cuts at: 0 290 929 1470 1512
Size: 290 639 541 42
Fragments arranged by size:
639 541 290 42
AhdI GACnn_n'nnGTC
Cuts at: 0 933 1512
Size: 933 579
AleI CACnn'nnGTG
Cuts at: 0 769 1512
Size: 769 743
AlwNI CAG_nnn'CTG
Cuts at: 0 218 249 840 1396 1512
Size: 218 31 591 556 116
Fragments arranged by size:
591 556 218 116 31
ApaI G_GGCC'C
Cuts at: 0 386 1384 1512
Size: 386 998 128
Fragments arranged by size:
998 386 128
ApaLI G'TGCA_C
Cuts at: 0 1215 1512
Size: 1215 297
AvaI C'yCGr_G
Cuts at: 0 74 1512
Size: 74 1438
BanI G'GyrC_C
Cuts at: 0 467 527 682 859 1018 1174 1247 1512
Size: 467 60 155 177 159 156 73 265
Fragments arranged by size:
467 265 177 159 156 155 73 60
BanII G_rGCy'C
Cuts at: 0 13 386 596 1384 1512
Size: 13 373 210 788 128
Fragments arranged by size:
788 373 210 128 13
BbeI G_GCGC'C
Cuts at: 0 471 686 863 1022 1512
Size: 471 215 177 159 490
Fragments arranged by size:
490 471 215 177 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 1155 1512
Size: 1155 357
BbvCI CC'TCA_GC
Cuts at: 0 1312 1512
Size: 1312 200
BglI GCCn_nnn'nGGC
Cuts at: 0 246 1512
Size: 246 1266
BlpI GC'TnA_GC
Cuts at: 0 590 1512
Size: 590 922
Bme1580I G_kGCm'C
Cuts at: 0 154 386 435 530 984 1177 1219 1250
Size: 154 232 49 95 454 193 42 31
Cuts at: 1250 1384 1512
Size: 134 128
Fragments arranged by size:
454 232 193 154 134 128 95 49
42 31
BmgBI CAC'GTC
Cuts at: 0 791 819 930 1512
Size: 791 28 111 582
Fragments arranged by size:
791 582 111 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 148 1384 1387 1512
Size: 148 1236 3 125
Fragments arranged by size:
1236 148 125 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1512
Size: 240 1272
Bpu10I CC'TnA_GC
Cuts at: 0 322 1312 1512
Size: 322 990 200
Fragments arranged by size:
990 322 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1512
Size: 493 1019
BsaI GGTCTCn'nnnn_
Cuts at: 0 1358 1512
Size: 1358 154
BsaAI yAC'GTr
Cuts at: 0 291 548 1473 1512
Size: 291 257 925 39
Fragments arranged by size:
925 291 257 39
BsaHI Gr'CG_yC
Cuts at: 0 4 468 683 860 1019 1512
Size: 4 464 215 177 159 493
Fragments arranged by size:
493 464 215 177 159 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1336 1366 1512
Size: 1336 30 146
Fragments arranged by size:
1336 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 384 1092 1357 1512
Size: 384 708 265 155
Fragments arranged by size:
708 384 265 155
BseYI C'CCAG_C
Cuts at: 0 45 126 1149 1367 1512
Size: 45 81 1023 218 145
Fragments arranged by size:
1023 218 145 81 45
BsiEI CG_ry'CG
Cuts at: 0 65 439 637 1275 1512
Size: 65 374 198 638 237
Fragments arranged by size:
638 374 237 198 65
BsiHKAI G_wGCw'C
Cuts at: 0 743 1219 1512
Size: 743 476 293
Fragments arranged by size:
743 476 293
Bsp1286I G_dGCh'C
Cuts at: 0 13 154 386 435 530 596 743 984
Size: 13 141 232 49 95 66 147 241
Cuts at: 984 1177 1219 1250 1384 1512
Size: 193 42 31 134 128
Fragments arranged by size:
241 232 193 147 141 134 128 95
66 49 42 31 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 252 638 667 1512
Size: 105 147 386 29 845
Fragments arranged by size:
845 386 147 105 29
BsrBI CCG'CTC
Cuts at: 0 270 1512
Size: 270 1242
BsrFI r'CCGG_y
Cuts at: 0 60 280 825 1153 1512
Size: 60 220 545 328 359
Fragments arranged by size:
545 359 328 220 60
BsrGI T'GTAC_A
Cuts at: 0 1004 1512
Size: 1004 508
BstXI CCAn_nnnn'nTGG
Cuts at: 0 891 1029 1512
Size: 891 138 483
Fragments arranged by size:
891 483 138
BtgI C'CryG_G
Cuts at: 0 109 488 920 1028 1126 1133 1512
Size: 109 379 432 108 98 7 379
Fragments arranged by size:
432 379 379 109 108 98 7
BtsI GCAGTG_nn'
Cuts at: 0 154 433 1512
Size: 154 279 1079
Fragments arranged by size:
1079 279 154
ClaI AT'CG_AT
Cuts at: 0 887 1512
Size: 887 625
DraIII CAC_nnn'GTG
Cuts at: 0 759 1512
Size: 759 753
DrdI GACnn_nn'nnGTC
Cuts at: 0 103 1512
Size: 103 1409
EaeI y'GGCC_r
Cuts at: 0 62 198 518 634 897 1112 1304 1512
Size: 62 136 320 116 263 215 192 208
Fragments arranged by size:
320 263 215 208 192 136 116 62
EagI C'GGCC_G
Cuts at: 0 62 634 1512
Size: 62 572 878
Fragments arranged by size:
878 572 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 1512
Size: 109 1403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 271 643 862 1512
Size: 240 31 372 219 650
Fragments arranged by size:
650 372 240 219 31
EcoO109I rG'GnC_Cy
Cuts at: 0 50 79 165 382 411 1290 1512
Size: 50 29 86 217 29 879 222
Fragments arranged by size:
879 222 217 86 50 29 29
FspI TGC'GCA
Cuts at: 0 811 1512
Size: 811 701
HaeII r_GCGC'y
Cuts at: 0 471 686 863 1022 1052 1160 1319 1512
Size: 471 215 177 159 30 108 159 193
Fragments arranged by size:
471 215 193 177 159 159 108 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 919 951 1282 1314 1336 1368
Size: 379 32 508 32 331 32 22 32
Cuts at: 1368 1512
Size: 144
Fragments arranged by size:
508 379 331 144 32 32 32 32
22
HincII GTy'rAC
Cuts at: 0 330 1512
Size: 330 1182
KasI G'GCGC_C
Cuts at: 0 467 682 859 1018 1512
Size: 467 215 177 159 494
Fragments arranged by size:
494 467 215 177 159
MscI TGG'CCA
Cuts at: 0 520 1512
Size: 520 992
MslI CAynn'nnrTG
Cuts at: 0 550 769 889 1512
Size: 550 219 120 623
Fragments arranged by size:
623 550 219 120
MspA1I CmG'CkG
Cuts at: 0 39 177 215 524 589 647 720 1280
Size: 39 138 38 309 65 58 73 560
Cuts at: 1280 1512
Size: 232
Fragments arranged by size:
560 309 232 138 73 65 58 39
38
NaeI GCC'GGC
Cuts at: 0 282 1155 1512
Size: 282 873 357
Fragments arranged by size:
873 357 282
NarI GG'CG_CC
Cuts at: 0 468 683 860 1019 1512
Size: 468 215 177 159 493
Fragments arranged by size:
493 468 215 177 159
NcoI C'CATG_G
Cuts at: 0 1028 1512
Size: 1028 484
NgoMIV G'CCGG_C
Cuts at: 0 280 1153 1512
Size: 280 873 359
Fragments arranged by size:
873 359 280
NspI r_CATG'y
Cuts at: 0 1005 1512
Size: 1005 507
PasI CC'CwG_GG
Cuts at: 0 364 1512
Size: 364 1148
PflMI CCAn_nnn'nTGG
Cuts at: 0 1396 1512
Size: 1396 116
PmlI CAC'GTG
Cuts at: 0 548 1473 1512
Size: 548 925 39
Fragments arranged by size:
925 548 39
PpuMI rG'GwC_Cy
Cuts at: 0 165 411 1290 1512
Size: 165 246 879 222
Fragments arranged by size:
879 246 222 165
PspOMI G'GGCC_C
Cuts at: 0 382 1380 1512
Size: 382 998 132
Fragments arranged by size:
998 382 132
PstI C_TGCA'G
Cuts at: 0 149 1512
Size: 149 1363
SanDI GG'GwC_CC
Cuts at: 0 165 1512
Size: 165 1347
SfcI C'TryA_G
Cuts at: 0 145 1035 1512
Size: 145 890 477
Fragments arranged by size:
890 477 145
SfoI GGC'GCC
Cuts at: 0 469 684 861 1020 1512
Size: 469 215 177 159 492
Fragments arranged by size:
492 469 215 177 159
SmlI C'TyrA_G
Cuts at: 0 508 1512
Size: 508 1004
StuI AGG'CCT
Cuts at: 0 568 1512
Size: 568 944
StyI C'CwwG_G
Cuts at: 0 191 1014 1028 1512
Size: 191 823 14 484
Fragments arranged by size:
823 484 191 14
TatI w'GTAC_w
Cuts at: 0 1004 1512
Size: 1004 508
Tth111I GACn'n_nGTC
Cuts at: 0 789 1512
Size: 789 723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 888 1512
Size: 702 186 624
Fragments arranged by size:
702 624 186
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BsrGI BstXI BtgI BtsI ClaI
DraIII DrdI EaeI EagI EciI Eco57MI EcoO109I FspI
HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PmlI PpuMI
PspOMI PstI SanDI SfcI SfoI SmlI StuI StyI
TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BmtI BplI BsaBI BsaWI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
CspCI DraI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI XbaI XhoI XmaI XmnI
ZraI