Restriction map for
pF1KB9475
(Linear) (Six-base) MAPSORT of: KIBB9475.seq Check: 9685 from: 1 to: 1029
KIBB9475.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 140 674 727 764 785 801 1029
Size: 106 34 534 53 37 21 16 228
Fragments arranged by size:
534 228 106 53 37 34 21 16
AflIII A'CryG_T
Cuts at: 0 880 1029
Size: 880 149
AhdI GACnn_n'nnGTC
Cuts at: 0 795 1029
Size: 795 234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 880 1029
Size: 880 149
AlwNI CAG_nnn'CTG
Cuts at: 0 75 705 1029
Size: 75 630 324
Fragments arranged by size:
630 324 75
ApoI r'AATT_y
Cuts at: 0 1024 1029
Size: 1024 5
BanI G'GyrC_C
Cuts at: 0 822 1029
Size: 822 207
BanII G_rGCy'C
Cuts at: 0 82 1029
Size: 82 947
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 83 791 1029
Size: 34 49 708 238
Fragments arranged by size:
708 238 49 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 408 1029
Size: 408 621
BglII A'GATC_T
Cuts at: 0 15 1029
Size: 15 1014
BmgBI CAC'GTC
Cuts at: 0 883 1029
Size: 883 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 1029
Size: 41 988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 1029
Size: 147 882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 622 1029
Size: 263 359 407
Fragments arranged by size:
407 359 263
BsaI GGTCTCn'nnnn_
Cuts at: 0 223 1029
Size: 223 806
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 1029
Size: 676 30 323
Fragments arranged by size:
676 323 30
BseYI C'CCAG_C
Cuts at: 0 233 1029
Size: 233 796
BsiHKAI G_wGCw'C
Cuts at: 0 773 1029
Size: 773 256
Bsp1286I G_dGCh'C
Cuts at: 0 82 773 1029
Size: 82 691 256
Fragments arranged by size:
691 256 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 1029
Size: 147 882
BsrDI GCAATG_nn'
Cuts at: 0 468 1029
Size: 468 561
BstYI r'GATC_y
Cuts at: 0 15 1029
Size: 15 1014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 420 455 1029
Size: 420 35 574
Fragments arranged by size:
574 420 35
DraI TTT'AAA
Cuts at: 0 957 1029
Size: 957 72
DraIII CAC_nnn'GTG
Cuts at: 0 151 886 1029
Size: 151 735 143
Fragments arranged by size:
735 151 143
EaeI y'GGCC_r
Cuts at: 0 28 1029
Size: 28 1001
EarI CTCTTCn'nnn_
Cuts at: 0 116 660 836 1029
Size: 116 544 176 193
Fragments arranged by size:
544 193 176 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 140 263 622 674 727 764 785
Size: 106 34 123 359 52 53 37 21
Cuts at: 785 801 1029
Size: 16 228
Fragments arranged by size:
359 228 123 106 53 52 37 34
21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 830 1029
Size: 830 199
EcoRV GAT'ATC
Cuts at: 0 294 1029
Size: 294 735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 195 1029
Size: 195 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1029
Size: 781 32 216
Fragments arranged by size:
781 216 32
HindIII A'AGCT_T
Cuts at: 0 177 1029
Size: 177 852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1029
Size: 967 62
MscI TGG'CCA
Cuts at: 0 30 1029
Size: 30 999
MspA1I CmG'CkG
Cuts at: 0 538 1029
Size: 538 491
NspI r_CATG'y
Cuts at: 0 451 674 1029
Size: 451 223 355
Fragments arranged by size:
451 355 223
PshAI GACnn'nnGTC
Cuts at: 0 632 1029
Size: 632 397
PstI C_TGCA'G
Cuts at: 0 207 856 1029
Size: 207 649 173
Fragments arranged by size:
649 207 173
PvuII CAG'CTG
Cuts at: 0 538 1029
Size: 538 491
SapI GCTCTTCn'nnn_
Cuts at: 0 660 1029
Size: 660 369
SexAI A'CCwGG_T
Cuts at: 0 392 1029
Size: 392 637
SfcI C'TryA_G
Cuts at: 0 203 852 1029
Size: 203 649 177
Fragments arranged by size:
649 203 177
StyI C'CwwG_G
Cuts at: 0 96 1029
Size: 96 933
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 1029
Size: 444 585
TatI w'GTAC_w
Cuts at: 0 455 1029
Size: 455 574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 207 239 676 708 1029
Size: 207 32 437 32 321
Fragments arranged by size:
437 321 207 32 32
XbaI T'CTAG_A
Cuts at: 0 552 1029
Size: 552 477
Enzymes that do cut:
AcuI AflIII AhdI AlfI AlwNI ApoI BanI BanII
BbsI BciVI BglII BmgBI BmrI BplI BpmI BsaI
BsaXI BseYI BsiHKAI Bsp1286I BspMI BsrDI BstYI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoRV FalI
Hin4I HindIII MmeI MscI MspA1I NspI PshAI PstI
PvuII SapI SexAI SfcI StyI TaqII TatI TstI
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmtI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DrdI EagI EciI EcoICRI EcoO109I EcoRI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI
XmaI XmnI ZraI