Restriction map for
pF1KB9477
(Linear) (Six-base) MAPSORT of: KIBB9477.seq Check: 8580 from: 1 to: 999
KIBB9477.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AatII G_ACGT'C
Cuts at: 0 962 999
Size: 962 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 999
Size: 646 353
AleI CACnn'nnGTG
Cuts at: 0 859 999
Size: 859 140
AlwNI CAG_nnn'CTG
Cuts at: 0 555 999
Size: 555 444
ApaI G_GGCC'C
Cuts at: 0 127 748 999
Size: 127 621 251
Fragments arranged by size:
621 251 127
AvaI C'yCGr_G
Cuts at: 0 427 605 740 999
Size: 427 178 135 259
Fragments arranged by size:
427 259 178 135
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 813 846 999
Size: 813 33 153
Fragments arranged by size:
813 153 33
BanI G'GyrC_C
Cuts at: 0 30 967 999
Size: 30 937 32
Fragments arranged by size:
937 32 30
BanII G_rGCy'C
Cuts at: 0 127 434 748 999
Size: 127 307 314 251
Fragments arranged by size:
314 307 251 127
BbeI G_GCGC'C
Cuts at: 0 34 971 999
Size: 34 937 28
Fragments arranged by size:
937 34 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 477 511 633 667 999
Size: 477 34 122 34 332
Fragments arranged by size:
477 332 122 34 34
BclI T'GATC_A
Cuts at: 0 659 999
Size: 659 340
BglI GCCn_nnn'nGGC
Cuts at: 0 560 689 999
Size: 560 129 310
Fragments arranged by size:
560 310 129
BlpI GC'TnA_GC
Cuts at: 0 59 999
Size: 59 940
Bme1580I G_kGCm'C
Cuts at: 0 127 712 748 999
Size: 127 585 36 251
Fragments arranged by size:
585 251 127 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 715 999
Size: 22 693 284
Fragments arranged by size:
693 284 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 835 999
Size: 376 459 164
Fragments arranged by size:
459 376 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 999
Size: 577 422
BsaAI yAC'GTr
Cuts at: 0 378 999
Size: 378 621
BsaHI Gr'CG_yC
Cuts at: 0 31 959 968 980 999
Size: 31 928 9 12 19
Fragments arranged by size:
928 31 19 12 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 999
Size: 67 932
BseYI C'CCAG_C
Cuts at: 0 6 78 153 185 558 867 921 999
Size: 6 72 75 32 373 309 54 78
Fragments arranged by size:
373 309 78 75 72 54 32 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 999
Size: 282 717
BsiEI CG_ry'CG
Cuts at: 0 993 999
Size: 993 6
Bsp1286I G_dGCh'C
Cuts at: 0 127 434 712 748 999
Size: 127 307 278 36 251
Fragments arranged by size:
307 278 251 127 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 999
Size: 818 181
BsrFI r'CCGG_y
Cuts at: 0 566 999
Size: 566 433
BssSI C'ACGA_G
Cuts at: 0 172 999
Size: 172 827
BstAPI GCAn_nnn'nTGC
Cuts at: 0 555 999
Size: 555 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 861 999
Size: 861 138
BstYI r'GATC_y
Cuts at: 0 762 999
Size: 762 237
BtgI C'CryG_G
Cuts at: 0 69 195 752 857 999
Size: 69 126 557 105 142
Fragments arranged by size:
557 142 126 105 69
DraIII CAC_nnn'GTG
Cuts at: 0 318 999
Size: 318 681
DrdI GACnn_nn'nnGTC
Cuts at: 0 949 985 999
Size: 949 36 14
Fragments arranged by size:
949 36 14
EaeI y'GGCC_r
Cuts at: 0 210 681 714 851 899 990 999
Size: 210 471 33 137 48 91 9
Fragments arranged by size:
471 210 137 91 48 33 9
EagI C'GGCC_G
Cuts at: 0 990 999
Size: 990 9
EarI CTCTTCn'nnn_
Cuts at: 0 776 999
Size: 776 223
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 122 259 999
Size: 36 86 137 740
Fragments arranged by size:
740 137 86 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 646 835 999
Size: 376 270 189 164
Fragments arranged by size:
376 270 189 164
EcoO109I rG'GnC_Cy
Cuts at: 0 97 123 124 722 744 745 906 999
Size: 97 26 1 598 22 1 161 93
Fragments arranged by size:
598 161 97 93 26 22 1 1
HaeII r_GCGC'y
Cuts at: 0 34 971 999
Size: 34 937 28
Fragments arranged by size:
937 34 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 999
Size: 754 32 213
Fragments arranged by size:
754 213 32
KasI G'GCGC_C
Cuts at: 0 30 967 999
Size: 30 937 32
Fragments arranged by size:
937 32 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 999
Size: 395 604
MslI CAynn'nnrTG
Cuts at: 0 517 859 999
Size: 517 342 140
Fragments arranged by size:
517 342 140
MspA1I CmG'CkG
Cuts at: 0 6 189 676 999
Size: 6 183 487 323
Fragments arranged by size:
487 323 183 6
NaeI GCC'GGC
Cuts at: 0 568 999
Size: 568 431
NarI GG'CG_CC
Cuts at: 0 31 968 999
Size: 31 937 31
Fragments arranged by size:
937 31 31
NcoI C'CATG_G
Cuts at: 0 195 857 999
Size: 195 662 142
Fragments arranged by size:
662 195 142
NgoMIV G'CCGG_C
Cuts at: 0 566 999
Size: 566 433
PflMI CCAn_nnn'nTGG
Cuts at: 0 70 318 999
Size: 70 248 681
Fragments arranged by size:
681 248 70
PmlI CAC'GTG
Cuts at: 0 378 999
Size: 378 621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 999
Size: 277 32 690
Fragments arranged by size:
690 277 32
PpuMI rG'GwC_Cy
Cuts at: 0 722 999
Size: 722 277
PspOMI G'GGCC_C
Cuts at: 0 123 744 999
Size: 123 621 255
Fragments arranged by size:
621 255 123
PvuII CAG'CTG
Cuts at: 0 189 999
Size: 189 810
SanDI GG'GwC_CC
Cuts at: 0 722 999
Size: 722 277
SfoI GGC'GCC
Cuts at: 0 32 969 999
Size: 32 937 30
Fragments arranged by size:
937 32 30
SmaI CCC'GGG
Cuts at: 0 742 999
Size: 742 257
SmlI C'TyrA_G
Cuts at: 0 592 999
Size: 592 407
StuI AGG'CCT
Cuts at: 0 265 649 999
Size: 265 384 350
Fragments arranged by size:
384 350 265
StyI C'CwwG_G
Cuts at: 0 195 857 983 999
Size: 195 662 126 16
Fragments arranged by size:
662 195 126 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 325 913 999
Size: 199 126 588 86
Fragments arranged by size:
588 199 126 86
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 193 798 864 999
Size: 193 605 66 135
Fragments arranged by size:
605 193 135 66
XmaI C'CCGG_G
Cuts at: 0 740 999
Size: 740 259
ZraI GAC'GTC
Cuts at: 0 960 999
Size: 960 39
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI AvaI BaeI BanI
BanII BbeI BcgI BclI BglI BlpI Bme1580I BmrI
BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI BtgI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV PflMI PmlI PpiI PpuMI PspOMI PvuII
SanDI SfoI SmaI SmlI StuI StyI TaqII XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbsI BbvCI BciVI BfrBI BglII BmgBI
BmtI BplI Bpu10I BsaI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmnI