Restriction map for
pF1KB9480
(Linear) (Six-base) MAPSORT of: KIBB9480.seq Check: 9047 from: 1 to: 2496
KIBB9480.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 975 1171 1927 2496
Size: 975 196 756 569
Fragments arranged by size:
975 756 569 196
AccI GT'mk_AC
Cuts at: 0 659 672 1484 1973 2496
Size: 659 13 812 489 523
Fragments arranged by size:
812 659 523 489 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 381 1048 1147 1981 2496
Size: 269 112 667 99 834 515
Fragments arranged by size:
834 667 515 269 112 99
AflIII A'CryG_T
Cuts at: 0 1139 2496
Size: 1139 1357
AgeI A'CCGG_T
Cuts at: 0 311 2496
Size: 311 2185
AhdI GACnn_n'nnGTC
Cuts at: 0 2454 2496
Size: 2454 42
AleI CACnn'nnGTG
Cuts at: 0 2140 2496
Size: 2140 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 535 2496
Size: 112 423 1961
Fragments arranged by size:
1961 423 112
AlwNI CAG_nnn'CTG
Cuts at: 0 105 1026 2248 2325 2496
Size: 105 921 1222 77 171
Fragments arranged by size:
1222 921 171 105 77
ApaLI G'TGCA_C
Cuts at: 0 445 2496
Size: 445 2051
ApoI r'AATT_y
Cuts at: 0 1642 2496
Size: 1642 854
AvaI C'yCGr_G
Cuts at: 0 2031 2496
Size: 2031 465
BanI G'GyrC_C
Cuts at: 0 28 1696 2496
Size: 28 1668 800
Fragments arranged by size:
1668 800 28
BanII G_rGCy'C
Cuts at: 0 1946 2364 2374 2387 2496
Size: 1946 418 10 13 109
Fragments arranged by size:
1946 418 109 13 10
BbeI G_GCGC'C
Cuts at: 0 1700 2496
Size: 1700 796
BbsI GAAGACnn'nnnn_
Cuts at: 0 1040 1881 2496
Size: 1040 841 615
Fragments arranged by size:
1040 841 615
BbvCI CC'TCA_GC
Cuts at: 0 1652 1725 2496
Size: 1652 73 771
Fragments arranged by size:
1652 771 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 789 823 2496
Size: 789 34 1673
Fragments arranged by size:
1673 789 34
BclI T'GATC_A
Cuts at: 0 929 2496
Size: 929 1567
BglI GCCn_nnn'nGGC
Cuts at: 0 1941 2496
Size: 1941 555
Bme1580I G_kGCm'C
Cuts at: 0 31 449 1937 2214 2496
Size: 31 418 1488 277 282
Fragments arranged by size:
1488 418 282 277 31
BmgBI CAC'GTC
Cuts at: 0 2126 2181 2496
Size: 2126 55 315
Fragments arranged by size:
2126 315 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 34 1266 1422 2496
Size: 34 1232 156 1074
Fragments arranged by size:
1232 1074 156 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1284 2496
Size: 1284 1212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 568 886 1036 1334 2496
Size: 321 247 318 150 298 1162
Fragments arranged by size:
1162 321 318 298 247 150
Bpu10I CC'TnA_GC
Cuts at: 0 1652 1725 2318 2373 2496
Size: 1652 73 593 55 123
Fragments arranged by size:
1652 593 123 73 55
BsaHI Gr'CG_yC
Cuts at: 0 119 497 1697 2432 2496
Size: 119 378 1200 735 64
Fragments arranged by size:
1200 735 378 119 64
BsaWI w'CCGG_w
Cuts at: 0 185 311 2496
Size: 185 126 2185
Fragments arranged by size:
2185 185 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1321 2425 2496
Size: 1321 1104 71
Fragments arranged by size:
1321 1104 71
BseYI C'CCAG_C
Cuts at: 0 1593 2496
Size: 1593 903
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 783 1143 1189 2496
Size: 604 179 360 46 1307
Fragments arranged by size:
1307 604 360 179 46
BsiEI CG_ry'CG
Cuts at: 0 1811 2496
Size: 1811 685
BsiHKAI G_wGCw'C
Cuts at: 0 449 1265 2234 2374 2496
Size: 449 816 969 140 122
Fragments arranged by size:
969 816 449 140 122
BsmBI CGTCTCn'nnnn_
Cuts at: 0 328 2496
Size: 328 2168
Bsp1286I G_dGCh'C
Cuts at: 0 31 449 1265 1937 1946 2214 2234 2364
Size: 31 418 816 672 9 268 20 130
Cuts at: 2364 2374 2387 2496
Size: 10 13 109
Fragments arranged by size:
816 672 418 268 130 109 31 20
13 10 9
BspEI T'CCGG_A
Cuts at: 0 185 2496
Size: 185 2311
BspHI T'CATG_A
Cuts at: 0 1901 2496
Size: 1901 595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 551 617 975 1171 1760 1927 2009 2496
Size: 551 66 358 196 589 167 82 487
Fragments arranged by size:
589 551 487 358 196 167 82 66
BsrBI CCG'CTC
Cuts at: 0 876 2496
Size: 876 1620
BsrDI GCAATG_nn'
Cuts at: 0 581 1718 2271 2496
Size: 581 1137 553 225
Fragments arranged by size:
1137 581 553 225
BsrFI r'CCGG_y
Cuts at: 0 311 2004 2496
Size: 311 1693 492
Fragments arranged by size:
1693 492 311
BsrGI T'GTAC_A
Cuts at: 0 1778 1949 2496
Size: 1778 171 547
Fragments arranged by size:
1778 547 171
BssHII G'CGCG_C
Cuts at: 0 1801 2496
Size: 1801 695
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 2496
Size: 105 2391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 1398 2496
Size: 132 1266 1098
Fragments arranged by size:
1266 1098 132
BstYI r'GATC_y
Cuts at: 0 416 2024 2496
Size: 416 1608 472
Fragments arranged by size:
1608 472 416
BtgI C'CryG_G
Cuts at: 0 1167 1370 1745 2496
Size: 1167 203 375 751
Fragments arranged by size:
1167 751 375 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 452 2298 2496
Size: 452 1846 198
Fragments arranged by size:
1846 452 198
BtsI GCAGTG_nn'
Cuts at: 0 160 2279 2496
Size: 160 2119 217
Fragments arranged by size:
2119 217 160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 927 962 2496
Size: 927 35 1534
Fragments arranged by size:
1534 927 35
EaeI y'GGCC_r
Cuts at: 0 17 948 1808 2006 2054 2496
Size: 17 931 860 198 48 442
Fragments arranged by size:
931 860 442 198 48 17
EagI C'GGCC_G
Cuts at: 0 1808 2496
Size: 1808 688
EarI CTCTTCn'nnn_
Cuts at: 0 730 1327 1339 1621 2080 2092 2496
Size: 730 597 12 282 459 12 404
Fragments arranged by size:
730 597 459 404 282 12 12
EcoICRI GAG'CTC
Cuts at: 0 2372 2496
Size: 2372 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 321 381 568 886 1036 1048 1147
Size: 269 52 60 187 318 150 12 99
Cuts at: 1147 1334 1981 2496
Size: 187 647 515
Fragments arranged by size:
647 515 318 269 187 187 150 99
60 52 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 2496
Size: 187 2309
EcoO109I rG'GnC_Cy
Cuts at: 0 102 540 2496
Size: 102 438 1956
Fragments arranged by size:
1956 438 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 1662 2496
Size: 906 756 834
Fragments arranged by size:
906 834 756
FspI TGC'GCA
Cuts at: 0 1759 2104 2496
Size: 1759 345 392
Fragments arranged by size:
1759 392 345
FspAI rTGC'GCAy
Cuts at: 0 2104 2496
Size: 2104 392
HaeII r_GCGC'y
Cuts at: 0 77 1700 2496
Size: 77 1623 796
Fragments arranged by size:
1623 796 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1268 1300 2374 2406 2440 2472 2496
Size: 1268 32 1074 32 34 32 24
Fragments arranged by size:
1268 1074 34 32 32 32 24
HincII GTy'rAC
Cuts at: 0 348 528 2496
Size: 348 180 1968
Fragments arranged by size:
1968 348 180
KasI G'GCGC_C
Cuts at: 0 1696 2496
Size: 1696 800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1238 2496
Size: 1238 1258
MscI TGG'CCA
Cuts at: 0 19 950 2496
Size: 19 931 1546
Fragments arranged by size:
1546 931 19
MslI CAynn'nnrTG
Cuts at: 0 1369 1570 1780 1930 1951 2128 2140 2496
Size: 1369 201 210 150 21 177 12 356
Fragments arranged by size:
1369 356 210 201 177 150 21 12
MspA1I CmG'CkG
Cuts at: 0 465 1625 2496
Size: 465 1160 871
Fragments arranged by size:
1160 871 465
NaeI GCC'GGC
Cuts at: 0 2006 2496
Size: 2006 490
NarI GG'CG_CC
Cuts at: 0 1697 2496
Size: 1697 799
NcoI C'CATG_G
Cuts at: 0 1370 1745 2496
Size: 1370 375 751
Fragments arranged by size:
1370 751 375
NgoMIV G'CCGG_C
Cuts at: 0 2004 2496
Size: 2004 492
NspI r_CATG'y
Cuts at: 0 669 1956 2496
Size: 669 1287 540
Fragments arranged by size:
1287 669 540
PflMI CCAn_nnn'nTGG
Cuts at: 0 2052 2496
Size: 2052 444
PfoI T'CCnGG_A
Cuts at: 0 419 2496
Size: 419 2077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1663 1695 2496
Size: 1663 32 801
Fragments arranged by size:
1663 801 32
PpuMI rG'GwC_Cy
Cuts at: 0 540 2496
Size: 540 1956
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 544 576 2496
Size: 544 32 1920
Fragments arranged by size:
1920 544 32
PstI C_TGCA'G
Cuts at: 0 101 1195 2330 2496
Size: 101 1094 1135 166
Fragments arranged by size:
1135 1094 166 101
SacI G_AGCT'C
Cuts at: 0 2374 2496
Size: 2374 122
SapI GCTCTTCn'nnn_
Cuts at: 0 2092 2496
Size: 2092 404
SbfI CC_TGCA'GG
Cuts at: 0 101 2496
Size: 101 2395
ScaI AGT'ACT
Cuts at: 0 1003 2496
Size: 1003 1493
SexAI A'CCwGG_T
Cuts at: 0 1967 2045 2496
Size: 1967 78 451
Fragments arranged by size:
1967 451 78
SfcI C'TryA_G
Cuts at: 0 69 97 561 660 1191 1908 2326 2496
Size: 69 28 464 99 531 717 418 170
Fragments arranged by size:
717 531 464 418 170 99 69 28
SfoI GGC'GCC
Cuts at: 0 1698 2496
Size: 1698 798
SphI G_CATG'C
Cuts at: 0 669 2496
Size: 669 1827
StuI AGG'CCT
Cuts at: 0 1769 1843 2496
Size: 1769 74 653
Fragments arranged by size:
1769 653 74
StyI C'CwwG_G
Cuts at: 0 56 218 1370 1745 2496
Size: 56 162 1152 375 751
Fragments arranged by size:
1152 751 375 162 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 275 1441 2496
Size: 275 1166 1055
Fragments arranged by size:
1166 1055 275
TatI w'GTAC_w
Cuts at: 0 887 1001 1778 1949 2496
Size: 887 114 777 171 547
Fragments arranged by size:
887 777 547 171 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 439 471 2496
Size: 439 32 2025
Fragments arranged by size:
2025 439 32
Tth111I GACn'n_nGTC
Cuts at: 0 1224 2496
Size: 1224 1272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 2185 2496
Size: 364 1821 311
Fragments arranged by size:
1821 364 311
XmnI GAAnn'nnTTC
Cuts at: 0 1132 2496
Size: 1132 1364
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AlfI
AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PflMI PfoI PpiI PpuMI PsrI PstI SacI
SapI SbfI ScaI SexAI SfcI SfoI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AloI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BglII
BlpI BmtI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI
BsmI BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraI
DraIII DrdI EciI EcoRI EcoRV FseI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PvuI PvuII RsrII SacII SalI SanDI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmaI ZraI