Restriction map for
pF1KB9482
(Linear) (Six-base) MAPSORT of: KIBB9482.seq Check: 7234 from: 1 to: 1551
KIBB9482.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1347 1551
Size: 1347 204
AccI GT'mk_AC
Cuts at: 0 1306 1551
Size: 1306 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1551
Size: 504 1047
AflIII A'CryG_T
Cuts at: 0 362 818 1472 1551
Size: 362 456 654 79
Fragments arranged by size:
654 456 362 79
AhdI GACnn_n'nnGTC
Cuts at: 0 819 1551
Size: 819 732
AlwNI CAG_nnn'CTG
Cuts at: 0 1293 1551
Size: 1293 258
ApaI G_GGCC'C
Cuts at: 0 893 1551
Size: 893 658
ApaLI G'TGCA_C
Cuts at: 0 418 1551
Size: 418 1133
AvaI C'yCGr_G
Cuts at: 0 277 1222 1504 1551
Size: 277 945 282 47
Fragments arranged by size:
945 282 277 47
AvrII C'CTAG_G
Cuts at: 0 9 1551
Size: 9 1542
BanI G'GyrC_C
Cuts at: 0 711 870 984 1551
Size: 711 159 114 567
Fragments arranged by size:
711 567 159 114
BanII G_rGCy'C
Cuts at: 0 893 1141 1551
Size: 893 248 410
Fragments arranged by size:
893 410 248
BbeI G_GCGC'C
Cuts at: 0 715 874 1551
Size: 715 159 677
Fragments arranged by size:
715 677 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 1105 1447 1551
Size: 1105 342 104
Fragments arranged by size:
1105 342 104
BbvCI CC'TCA_GC
Cuts at: 0 1074 1551
Size: 1074 477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 628 1234 1268 1551
Size: 594 34 606 34 283
Fragments arranged by size:
606 594 283 34 34
BclI T'GATC_A
Cuts at: 0 1517 1551
Size: 1517 34
BglI GCCn_nnn'nGGC
Cuts at: 0 406 475 1160 1551
Size: 406 69 685 391
Fragments arranged by size:
685 406 391 69
BlpI GC'TnA_GC
Cuts at: 0 1450 1551
Size: 1450 101
Bme1580I G_kGCm'C
Cuts at: 0 391 422 893 1551
Size: 391 31 471 658
Fragments arranged by size:
658 471 391 31
BmgBI CAC'GTC
Cuts at: 0 365 1353 1551
Size: 365 988 198
Fragments arranged by size:
988 365 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 1551
Size: 378 1173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1089 1551
Size: 1089 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 649 661 816 1551
Size: 160 489 12 155 735
Fragments arranged by size:
735 489 160 155 12
Bpu10I CC'TnA_GC
Cuts at: 0 1074 1551
Size: 1074 477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 247 529 1125 1474 1528 1551
Size: 171 76 282 596 349 54 23
Fragments arranged by size:
596 349 282 171 76 54 23
BsaAI yAC'GTr
Cuts at: 0 122 1551
Size: 122 1429
BsaHI Gr'CG_yC
Cuts at: 0 712 871 1102 1551
Size: 712 159 231 449
Fragments arranged by size:
712 449 231 159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 1551
Size: 634 30 887
Fragments arranged by size:
887 634 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 193 244 487 496 499 510 586
Size: 190 3 51 243 9 3 11 76
Cuts at: 586 589 691 898 940 949 952 967 979
Size: 3 102 207 42 9 3 15 12
Cuts at: 979 982 985 988 1042 1060 1062 1182 1551
Size: 3 3 3 54 18 2 120 369
Fragments arranged by size:
369 243 207 190 120 102 76 54
51 42 18 15 12 11 9 9
3 3 3 3 3 3 3 2
BseYI C'CCAG_C
Cuts at: 0 1168 1313 1551
Size: 1168 145 238
Fragments arranged by size:
1168 238 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1551
Size: 1375 176
BsiHKAI G_wGCw'C
Cuts at: 0 422 1141 1551
Size: 422 719 410
Fragments arranged by size:
719 422 410
Bsp1286I G_dGCh'C
Cuts at: 0 391 422 893 1141 1551
Size: 391 31 471 248 410
Fragments arranged by size:
471 410 391 248 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1347 1424 1551
Size: 1347 77 127
Fragments arranged by size:
1347 127 77
BsrBI CCG'CTC
Cuts at: 0 144 1119 1551
Size: 144 975 432
Fragments arranged by size:
975 432 144
BstYI r'GATC_y
Cuts at: 0 1484 1551
Size: 1484 67
Bsu36I CC'TnA_GG
Cuts at: 0 950 1551
Size: 950 601
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 439 797 1551
Size: 439 358 754
Fragments arranged by size:
754 439 358
BtsI GCAGTG_nn'
Cuts at: 0 210 464 1551
Size: 210 254 1087
Fragments arranged by size:
1087 254 210
DraIII CAC_nnn'GTG
Cuts at: 0 386 1551
Size: 386 1165
EaeI y'GGCC_r
Cuts at: 0 591 719 1551
Size: 591 128 832
Fragments arranged by size:
832 591 128
EarI CTCTTCn'nnn_
Cuts at: 0 842 1087 1551
Size: 842 245 464
Fragments arranged by size:
842 464 245
EcoICRI GAG'CTC
Cuts at: 0 1139 1551
Size: 1139 412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 504 649 661 816 1551
Size: 160 344 145 12 155 735
Fragments arranged by size:
735 344 160 155 145 12
EcoO109I rG'GnC_Cy
Cuts at: 0 13 398 889 1282 1413 1551
Size: 13 385 491 393 131 138
Fragments arranged by size:
491 393 385 138 131 13
FspI TGC'GCA
Cuts at: 0 616 1551
Size: 616 935
HaeII r_GCGC'y
Cuts at: 0 715 874 1551
Size: 715 159 677
Fragments arranged by size:
715 677 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 634 666 805 837 1551
Size: 349 32 253 32 139 32 714
Fragments arranged by size:
714 349 253 139 32 32 32
HincII GTy'rAC
Cuts at: 0 111 306 1307 1551
Size: 111 195 1001 244
Fragments arranged by size:
1001 244 195 111
HindIII A'AGCT_T
Cuts at: 0 220 1551
Size: 220 1331
KasI G'GCGC_C
Cuts at: 0 711 870 1551
Size: 711 159 681
Fragments arranged by size:
711 681 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1551
Size: 1013 538
MscI TGG'CCA
Cuts at: 0 721 1551
Size: 721 830
MspA1I CmG'CkG
Cuts at: 0 444 537 717 1346 1551
Size: 444 93 180 629 205
Fragments arranged by size:
629 444 205 180 93
NarI GG'CG_CC
Cuts at: 0 712 871 1551
Size: 712 159 680
Fragments arranged by size:
712 680 159
NspI r_CATG'y
Cuts at: 0 822 1551
Size: 822 729
PciI A'CATG_T
Cuts at: 0 818 1551
Size: 818 733
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 810 1551
Size: 627 183 741
Fragments arranged by size:
741 627 183
PpuMI rG'GwC_Cy
Cuts at: 0 1282 1413 1551
Size: 1282 131 138
Fragments arranged by size:
1282 138 131
PspOMI G'GGCC_C
Cuts at: 0 889 1551
Size: 889 662
PspXI vC'TCGA_Gb
Cuts at: 0 1222 1551
Size: 1222 329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 352 384 1551
Size: 352 32 1167
Fragments arranged by size:
1167 352 32
PstI C_TGCA'G
Cuts at: 0 1257 1551
Size: 1257 294
SacI G_AGCT'C
Cuts at: 0 1141 1551
Size: 1141 410
SalI G'TCGA_C
Cuts at: 0 1305 1551
Size: 1305 246
SanDI GG'GwC_CC
Cuts at: 0 1282 1551
Size: 1282 269
SapI GCTCTTCn'nnn_
Cuts at: 0 842 1551
Size: 842 709
SfcI C'TryA_G
Cuts at: 0 1253 1551
Size: 1253 298
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 406 1551
Size: 406 1145
SfoI GGC'GCC
Cuts at: 0 713 872 1551
Size: 713 159 679
Fragments arranged by size:
713 679 159
SmlI C'TyrA_G
Cuts at: 0 150 262 544 1140 1222 1489 1543 1551
Size: 150 112 282 596 82 267 54 8
Fragments arranged by size:
596 282 267 150 112 82 54 8
StuI AGG'CCT
Cuts at: 0 409 769 1069 1551
Size: 409 360 300 482
Fragments arranged by size:
482 409 360 300
StyI C'CwwG_G
Cuts at: 0 9 695 722 866 893 1551
Size: 9 686 27 144 27 658
Fragments arranged by size:
686 658 144 27 27 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 1002 1122 1353 1551
Size: 397 605 120 231 198
Fragments arranged by size:
605 397 231 198 120
Tth111I GACn'n_nGTC
Cuts at: 0 106 1551
Size: 106 1445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1294 1551
Size: 1294 257
XhoI C'TCGA_G
Cuts at: 0 1222 1551
Size: 1222 329
XmnI GAAnn'nnTTC
Cuts at: 0 517 1426 1551
Size: 517 909 125
Fragments arranged by size:
909 517 125
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BstYI Bsu36I BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I HincII HindIII KasI MmeI MscI MspA1I NarI
NspI PciI PflMI PpuMI PspOMI PspXI PsrI PstI
SacI SalI SanDI SapI SfcI SfiI SfoI SmlI
StuI StyI TaqII Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApoI AscI AseI AsiSI BaeI BamHI BciVI
BfrBI BglII BmtI BsaI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspAI HpaI KpnI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PvuI PvuII RsrII SacII SbfI ScaI SexAI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI XbaI XmaI ZraI