Restriction map for
pF1KB9485
(Linear) (Six-base) MAPSORT of: KIBB9485.seq Check: 4716 from: 1 to: 6189
KIBB9485.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4753 5316 6189
Size: 4753 563 873
Fragments arranged by size:
4753 873 563
AccI GT'mk_AC
Cuts at: 0 2794 4207 6189
Size: 2794 1413 1982
Fragments arranged by size:
2794 1982 1413
AclI AA'CG_TT
Cuts at: 0 1278 6189
Size: 1278 4911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 590 872 1256 1424 1727 2573 2864
Size: 309 281 282 384 168 303 846 291
Cuts at: 2864 3611 3737 4151 4205 4298 4967 5168 5615
Size: 747 126 414 54 93 669 201 447
Cuts at: 5615 5624 6189
Size: 9 565
Fragments arranged by size:
846 747 669 565 447 414 384 309
303 291 282 281 201 168 126 93
54 9
AflIII A'CryG_T
Cuts at: 0 4087 6189
Size: 4087 2102
AgeI A'CCGG_T
Cuts at: 0 1470 6189
Size: 1470 4719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1073 4598 6189
Size: 1073 3525 1591
Fragments arranged by size:
3525 1591 1073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 6189
Size: 219 32 5938
Fragments arranged by size:
5938 219 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1090 1675 2707 3808 4228 4893 5968 6189
Size: 1090 585 1032 1101 420 665 1075 221
Fragments arranged by size:
1101 1090 1075 1032 665 585 420 221
ApaLI G'TGCA_C
Cuts at: 0 2804 6189
Size: 2804 3385
ApoI r'AATT_y
Cuts at: 0 632 763 939 1737 2077 2199 2241 2314
Size: 632 131 176 798 340 122 42 73
Cuts at: 2314 2402 3534 3621 4023 4617 5092 5545 6141
Size: 88 1132 87 402 594 475 453 596
Cuts at: 6141 6189
Size: 48
Fragments arranged by size:
1132 798 632 596 594 475 453 402
340 176 131 122 88 87 73 48
42
AseI AT'TA_AT
Cuts at: 0 443 4291 5465 6189
Size: 443 3848 1174 724
Fragments arranged by size:
3848 1174 724 443
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 252 285 2276 2309 4775 4808 6189
Size: 252 33 1991 33 2466 33 1381
Fragments arranged by size:
2466 1991 1381 252 33 33 33
BanI G'GyrC_C
Cuts at: 0 103 1467 6189
Size: 103 1364 4722
Fragments arranged by size:
4722 1364 103
BanII G_rGCy'C
Cuts at: 0 1002 1899 3086 6180 6189
Size: 1002 897 1187 3094 9
Fragments arranged by size:
3094 1187 1002 897 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1218 2234 2604 2834 3870 4714 5336 6189
Size: 1218 1016 370 230 1036 844 622 853
Fragments arranged by size:
1218 1036 1016 853 844 622 370 230
BbvCI CC'TCA_GC
Cuts at: 0 911 2750 6189
Size: 911 1839 3439
Fragments arranged by size:
3439 1839 911
BciVI GTATCCnnnnn_n'
Cuts at: 0 4416 6189
Size: 4416 1773
BclI T'GATC_A
Cuts at: 0 39 5421 6189
Size: 39 5382 768
Fragments arranged by size:
5382 768 39
BfrBI ATG'CAT
Cuts at: 0 466 4390 6189
Size: 466 3924 1799
Fragments arranged by size:
3924 1799 466
BglI GCCn_nnn'nGGC
Cuts at: 0 3979 5275 6189
Size: 3979 1296 914
Fragments arranged by size:
3979 1296 914
BglII A'GATC_T
Cuts at: 0 672 694 1876 2136 5127 5407 6054 6189
Size: 672 22 1182 260 2991 280 647 135
Fragments arranged by size:
2991 1182 672 647 280 260 135 22
Bme1580I G_kGCm'C
Cuts at: 0 108 2808 6189
Size: 108 2700 3381
Fragments arranged by size:
3381 2700 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 2793 3434 3587 4238 6189
Size: 2793 641 153 651 1951
Fragments arranged by size:
2793 1951 651 641 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1175 1570 2309 2684 3416 5270 5333
Size: 78 1097 395 739 375 732 1854 63
Cuts at: 5333 5566 5921 6189
Size: 233 355 268
Fragments arranged by size:
1854 1097 739 732 395 375 355 268
233 78 63
Bpu10I CC'TnA_GC
Cuts at: 0 911 2750 3612 5888 6189
Size: 911 1839 862 2276 301
Fragments arranged by size:
2276 1839 911 862 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2156 3248 4389 6189
Size: 2156 1092 1141 1800
Fragments arranged by size:
2156 1800 1141 1092
BsaI GGTCTCn'nnnn_
Cuts at: 0 2515 6189
Size: 2515 3674
BsaAI yAC'GTr
Cuts at: 0 3093 6189
Size: 3093 3096
BsaBI GATnn'nnATC
Cuts at: 0 335 1385 2060 6189
Size: 335 1050 675 4129
Fragments arranged by size:
4129 1050 675 335
BsaHI Gr'CG_yC
Cuts at: 0 1109 3867 6189
Size: 1109 2758 2322
Fragments arranged by size:
2758 2322 1109
BsaWI w'CCGG_w
Cuts at: 0 195 1470 6189
Size: 195 1275 4719
Fragments arranged by size:
4719 1275 195
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 219 249 1428 1458 2664 2682 2694 2712
Size: 219 30 1179 30 1206 18 12 18
Cuts at: 2712 4430 4460 5919 5949 6189
Size: 1718 30 1459 30 240
Fragments arranged by size:
1718 1459 1206 1179 240 219 30 30
30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 811 814 1996 1999 2002 2020 2632
Size: 26 785 3 1182 3 3 18 612
Cuts at: 2632 2685 2738 2884 3251 3736 4956 6189
Size: 53 53 146 367 485 1220 1233
Fragments arranged by size:
1233 1220 1182 785 612 485 367 146
53 53 26 18 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2217 3244 5012 6189
Size: 2217 1027 1768 1177
Fragments arranged by size:
2217 1768 1177 1027
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1004 3950 4799 6189
Size: 895 109 2946 849 1390
Fragments arranged by size:
2946 1390 895 849 109
BsiHKAI G_wGCw'C
Cuts at: 0 856 1002 2808 3086 4949 6189
Size: 856 146 1806 278 1863 1240
Fragments arranged by size:
1863 1806 1240 856 278 146
Bsp1286I G_dGCh'C
Cuts at: 0 108 856 1002 1899 2808 3086 4949 6180
Size: 108 748 146 897 909 278 1863 1231
Cuts at: 6180 6189
Size: 9
Fragments arranged by size:
1863 1231 909 897 748 278 146 108
9
BspEI T'CCGG_A
Cuts at: 0 195 6189
Size: 195 5994
BspHI T'CATG_A
Cuts at: 0 4170 6189
Size: 4170 2019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 1084 4753 4771 5316 6189
Size: 600 484 3669 18 545 873
Fragments arranged by size:
3669 873 600 545 484 18
BsrDI GCAATG_nn'
Cuts at: 0 602 2410 3255 4390 6189
Size: 602 1808 845 1135 1799
Fragments arranged by size:
1808 1799 1135 845 602
BsrFI r'CCGG_y
Cuts at: 0 1470 4898 6189
Size: 1470 3428 1291
Fragments arranged by size:
3428 1470 1291
BssSI C'ACGA_G
Cuts at: 0 2518 6189
Size: 2518 3671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 379 1090 5095 6189
Size: 379 711 4005 1094
Fragments arranged by size:
4005 1094 711 379
BstBI TT'CG_AA
Cuts at: 0 1605 1761 6189
Size: 1605 156 4428
Fragments arranged by size:
4428 1605 156
BstEII G'GTnAC_C
Cuts at: 0 2833 6189
Size: 2833 3356
BstXI CCAn_nnnn'nTGG
Cuts at: 0 6100 6189
Size: 6100 89
BstYI r'GATC_y
Cuts at: 0 672 694 832 906 1876 2136 2728 3921
Size: 672 22 138 74 970 260 592 1193
Cuts at: 3921 5127 5407 6054 6189
Size: 1206 280 647 135
Fragments arranged by size:
1206 1193 970 672 647 592 280 260
138 135 74 22
BtgI C'CryG_G
Cuts at: 0 896 3745 6189
Size: 896 2849 2444
Fragments arranged by size:
2849 2444 896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 842 3407 6189
Size: 842 2565 2782
Fragments arranged by size:
2782 2565 842
BtsI GCAGTG_nn'
Cuts at: 0 1067 6189
Size: 1067 5122
DraI TTT'AAA
Cuts at: 0 5646 5765 6189
Size: 5646 119 424
Fragments arranged by size:
5646 424 119
DraIII CAC_nnn'GTG
Cuts at: 0 1819 1969 3595 6189
Size: 1819 150 1626 2594
Fragments arranged by size:
2594 1819 1626 150
EaeI y'GGCC_r
Cuts at: 0 2 899 6189
Size: 2 897 5290
Fragments arranged by size:
5290 897 2
EarI CTCTTCn'nnn_
Cuts at: 0 923 1703 1997 2861 2873 3092 3221 3490
Size: 923 780 294 864 12 219 129 269
Cuts at: 3490 3713 4012 4127 4339 6189
Size: 223 299 115 212 1850
Fragments arranged by size:
1850 923 864 780 299 294 269 223
219 212 129 115 12
EcoICRI GAG'CTC
Cuts at: 0 1000 3084 6189
Size: 1000 2084 3105
Fragments arranged by size:
3105 2084 1000
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 309 590 872 1175 1256 1424 1570
Size: 78 231 281 282 303 81 168 146
Cuts at: 1570 1727 2309 2573 2684 2864 3416 3611 3737
Size: 157 582 264 111 180 552 195 126
Cuts at: 3737 4151 4205 4298 4967 5168 5270 5333 5566
Size: 414 54 93 669 201 102 63 233
Cuts at: 5566 5615 5624 5921 6189
Size: 49 9 297 268
Fragments arranged by size:
669 582 552 414 303 297 282 281
268 264 233 231 201 195 180 168
157 146 126 111 102 93 81 78
63 54 49 9
EcoO109I rG'GnC_Cy
Cuts at: 0 16 3226 5557 5628 6189
Size: 16 3210 2331 71 561
Fragments arranged by size:
3210 2331 561 71 16
EcoRI G'AATT_C
Cuts at: 0 3621 4023 6189
Size: 3621 402 2166
Fragments arranged by size:
3621 2166 402
EcoRV GAT'ATC
Cuts at: 0 5144 6189
Size: 5144 1045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1318 3082 5149 6189
Size: 1318 1764 2067 1040
Fragments arranged by size:
2067 1764 1318 1040
HaeII r_GCGC'y
Cuts at: 0 714 6189
Size: 714 5475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 841 862 873 894 1410 1442 2569 2601
Size: 841 21 11 21 516 32 1127 32
Cuts at: 2601 2664 2696 3868 3900 4390 4422 5287 5319
Size: 63 32 1172 32 490 32 865 32
Cuts at: 5319 5917 5949 6189
Size: 598 32 240
Fragments arranged by size:
1172 1127 865 841 598 516 490 240
63 32 32 32 32 32 32 32
21 21 11
HindIII A'AGCT_T
Cuts at: 0 3894 4253 5179 6189
Size: 3894 359 926 1010
Fragments arranged by size:
3894 1010 926 359
MfeI C'AATT_G
Cuts at: 0 2359 2982 6189
Size: 2359 623 3207
Fragments arranged by size:
3207 2359 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 3490 3949 6189
Size: 195 3295 459 2240
Fragments arranged by size:
3295 2240 459 195
MscI TGG'CCA
Cuts at: 0 4 6189
Size: 4 6185
MslI CAynn'nnrTG
Cuts at: 0 871 1643 1808 4952 6189
Size: 871 772 165 3144 1237
Fragments arranged by size:
3144 1237 871 772 165
MspA1I CmG'CkG
Cuts at: 0 315 379 826 1195 2439 2446 2488 4035
Size: 315 64 447 369 1244 7 42 1547
Cuts at: 4035 4893 6189
Size: 858 1296
Fragments arranged by size:
1547 1296 1244 858 447 369 315 64
42 7
NcoI C'CATG_G
Cuts at: 0 3745 6189
Size: 3745 2444
NruI TCG'CGA
Cuts at: 0 638 6189
Size: 638 5551
NsiI A_TGCA'T
Cuts at: 0 468 4392 6189
Size: 468 3924 1797
Fragments arranged by size:
3924 1797 468
NspI r_CATG'y
Cuts at: 0 2426 3051 4091 6189
Size: 2426 625 1040 2098
Fragments arranged by size:
2426 2098 1040 625
PciI A'CATG_T
Cuts at: 0 4087 6189
Size: 4087 2102
PflMI CCAn_nnn'nTGG
Cuts at: 0 214 6189
Size: 214 5975
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 1619 1651 6189
Size: 219 32 1368 32 4538
Fragments arranged by size:
4538 1368 219 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 16 3226 5628 6189
Size: 16 3210 2402 561
Fragments arranged by size:
3210 2402 561 16
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5615 5647 6189
Size: 5615 32 542
Fragments arranged by size:
5615 542 32
PstI C_TGCA'G
Cuts at: 0 1095 4349 4750 6189
Size: 1095 3254 401 1439
Fragments arranged by size:
3254 1439 1095 401
PvuII CAG'CTG
Cuts at: 0 379 826 1195 2439 2446 2488 4035 4893
Size: 379 447 369 1244 7 42 1547 858
Cuts at: 4893 6189
Size: 1296
Fragments arranged by size:
1547 1296 1244 858 447 379 369 42
7
SacI G_AGCT'C
Cuts at: 0 1002 3086 6189
Size: 1002 2084 3103
Fragments arranged by size:
3103 2084 1002
SapI GCTCTTCn'nnn_
Cuts at: 0 2873 4127 6189
Size: 2873 1254 2062
Fragments arranged by size:
2873 2062 1254
SbfI CC_TGCA'GG
Cuts at: 0 4750 6189
Size: 4750 1439
ScaI AGT'ACT
Cuts at: 0 1437 6189
Size: 1437 4752
SexAI A'CCwGG_T
Cuts at: 0 3525 6189
Size: 3525 2664
SfcI C'TryA_G
Cuts at: 0 815 842 1003 1091 1324 1457 2332 3809
Size: 815 27 161 88 233 133 875 1477
Cuts at: 3809 4208 4345 4746 4787 6189
Size: 399 137 401 41 1402
Fragments arranged by size:
1477 1402 875 815 401 399 233 161
137 133 88 41 27
SmlI C'TyrA_G
Cuts at: 0 2171 3263 4368 6189
Size: 2171 1092 1105 1821
Fragments arranged by size:
2171 1821 1105 1092
SnaBI TAC'GTA
Cuts at: 0 3093 6189
Size: 3093 3096
SpeI A'CTAG_T
Cuts at: 0 5692 6189
Size: 5692 497
SphI G_CATG'C
Cuts at: 0 2426 3051 6189
Size: 2426 625 3138
Fragments arranged by size:
3138 2426 625
StyI C'CwwG_G
Cuts at: 0 363 3697 3745 6189
Size: 363 3334 48 2444
Fragments arranged by size:
3334 2444 363 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3063 3606 6139 6189
Size: 3063 543 2533 50
Fragments arranged by size:
3063 2533 543 50
TatI w'GTAC_w
Cuts at: 0 1435 2527 2743 3267 6189
Size: 1435 1092 216 524 2922
Fragments arranged by size:
2922 1435 1092 524 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 3788 3820 5441 5473 6189
Size: 230 32 3526 32 1621 32 716
Fragments arranged by size:
3526 1621 716 230 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 357 1437 1634 3065 4928 5129 6189
Size: 357 1080 197 1431 1863 201 1060
Fragments arranged by size:
1863 1431 1080 1060 357 201 197
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI BaeI BanI BanII BbsI
BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HindIII MfeI MmeI MscI MslI MspA1I NcoI NruI
NsiI NspI PciI PflMI PpiI PpuMI PsrI PstI
PvuII SacI SapI SbfI ScaI SexAI SfcI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AhdI AleI ApaI AscI
AsiSI AvaI AvrII BamHI BbeI BcgI BlpI BmgBI
BmtI BplI BsiEI BsiWI BsmI BsmBI BsrBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DrdI EagI EciI
EcoNI FseI FspI FspAI HincII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI PacI
PasI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SfiI SfoI SgfI
SgrAI SmaI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI