(Linear) (Six-base) MAPSORT of: KIBB9493.seq  Check: 4626  from: 1  to: 591



KIBB9493.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     221     591 
   Size:         88     133     370

  Fragments arranged by size: 

                 370     133      88
 
AflIII A'CryG_T

Cuts at:      0     165     591 
   Size:        165     426
 
ApoI r'AATT_y

Cuts at:      0     133     591 
   Size:        133     458
 
AvaI C'yCGr_G

Cuts at:      0     272     591 
   Size:        272     319
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     433     591 
   Size:        433     158
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     591 
   Size:         49     542
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     425     455     591 
   Size:        425      30     136

  Fragments arranged by size: 

                 425     136      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     409     591 
   Size:        409     182
 
BspHI T'CATG_A

Cuts at:      0     456     591 
   Size:        456     135
 
BsrDI GCAATG_nn'

Cuts at:      0     147     591 
   Size:        147     444
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     247     591 
   Size:        247     344
 
EarI CTCTTCn'nnn_

Cuts at:      0     293     591 
   Size:        293     298
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49      88     221     591 
   Size:         49      39     133     370

  Fragments arranged by size: 

                 370     133      49      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     303     396     591 
   Size:        303      93     195

  Fragments arranged by size: 

                 303     195      93
 
EcoRI G'AATT_C

Cuts at:      0     133     591 
   Size:        133     458
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     322     591 
   Size:        322     269
 
HaeII r_GCGC'y

Cuts at:      0      10     591 
   Size:         10     581
 
MfeI C'AATT_G

Cuts at:      0      50     591 
   Size:         50     541
 
MslI CAynn'nnrTG

Cuts at:      0     170     591 
   Size:        170     421
 
NspI r_CATG'y

Cuts at:      0     169     591 
   Size:        169     422
 
PciI A'CATG_T

Cuts at:      0     165     591 
   Size:        165     426
 
PspXI vC'TCGA_Gb

Cuts at:      0     272     591 
   Size:        272     319
 
SfcI C'TryA_G

Cuts at:      0     467     591 
   Size:        467     124
 
SmlI C'TyrA_G

Cuts at:      0     272     591 
   Size:        272     319
 
SspI AAT'ATT

Cuts at:      0     318     591 
   Size:        318     273
 
StyI C'CwwG_G

Cuts at:      0     306     591 
   Size:        306     285
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     537     591 
   Size:        537      54
 
XhoI C'TCGA_G

Cuts at:      0     272     591 
   Size:        272     319
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI     BplI     BpmI    BsaXI    BseRI
    BspHI    BsrDI   BstAPI     EarI  Eco57MI EcoO109I    EcoRI     FalI
    HaeII     MfeI     MslI     NspI     PciI    PspXI     SfcI     SmlI
     SspI     StyI    TaqII     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI