(Linear) (Six-base) MAPSORT of: KIBB9505.seq  Check: 370  from: 1  to: 675



KIBB9505.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AatII G_ACGT'C

Cuts at:      0     100     675 
   Size:        100     575
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     675 
   Size:        467     208
 
AflIII A'CryG_T

Cuts at:      0     420     675 
   Size:        420     255
 
AlwNI CAG_nnn'CTG

Cuts at:      0     457     675 
   Size:        457     218
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     100     675 
   Size:        100     575
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     213     675 
   Size:        213     462
 
BfrBI ATG'CAT

Cuts at:      0     247     675 
   Size:        247     428
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     345     675 
   Size:        345     330
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     675 
   Size:        620      55
 
BsaHI Gr'CG_yC

Cuts at:      0      97     675 
   Size:         97     578
 
BseYI C'CCAG_C

Cuts at:      0      72     675 
   Size:         72     603
 
BsiEI CG_ry'CG

Cuts at:      0      11     675 
   Size:         11     664
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     217     675 
   Size:        217     458
 
BsrDI GCAATG_nn'

Cuts at:      0     582     675 
   Size:        582      93
 
BsrFI r'CCGG_y

Cuts at:      0      10     675 
   Size:         10     665
 
BsrGI T'GTAC_A

Cuts at:      0     612     675 
   Size:        612      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     649     675 
   Size:        649      26
 
EaeI y'GGCC_r

Cuts at:      0       8     675 
   Size:          8     667
 
EagI C'GGCC_G

Cuts at:      0       8     675 
   Size:          8     667
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     675 
   Size:        467     208
 
NaeI GCC'GGC

Cuts at:      0      12     675 
   Size:         12     663
 
NdeI CA'TA_TG

Cuts at:      0     214     675 
   Size:        214     461
 
NgoMIV G'CCGG_C

Cuts at:      0      10     675 
   Size:         10     665
 
NsiI A_TGCA'T

Cuts at:      0     249     675 
   Size:        249     426
 
NspI r_CATG'y

Cuts at:      0     424     675 
   Size:        424     251
 
PciI A'CATG_T

Cuts at:      0     420     675 
   Size:        420     255
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     249     675 
   Size:        249     426
 
PstI C_TGCA'G

Cuts at:      0     158     675 
   Size:        158     517
 
SfcI C'TryA_G

Cuts at:      0     154     264     675 
   Size:        154     110     411

  Fragments arranged by size: 

                 411     154     110
 
SmlI C'TyrA_G

Cuts at:      0     635     675 
   Size:        635      40
 
SspI AAT'ATT

Cuts at:      0     186     675 
   Size:        186     489
 
TatI w'GTAC_w

Cuts at:      0     612     675 
   Size:        612      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     649     675 
   Size:        649      26
 
XmnI GAAnn'nnTTC

Cuts at:      0     515     675 
   Size:        515     160
 
ZraI GAC'GTC

Cuts at:      0      98     675 
   Size:         98     577
 

 Enzymes that do cut:

    AatII     AcuI   AflIII    AlwNI     BbsI    BciVI    BfrBI     BmrI
    BpuEI    BsaHI    BseYI    BsiEI    BspMI    BsrDI    BsrFI    BsrGI
    BstXI     EaeI     EagI  Eco57MI     NaeI     NdeI   NgoMIV     NsiI
     NspI     PciI    PflMI     PstI     SfcI     SmlI     SspI     TatI
     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NheI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI