Restriction map for
pF1KB9506
(Linear) (Six-base) MAPSORT of: KIBB9506.seq Check: 2601 from: 1 to: 1326
KIBB9506.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 339 1326
Size: 339 987
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1326
Size: 279 1047
AfeI AGC'GCT
Cuts at: 0 145 1326
Size: 145 1181
AleI CACnn'nnGTG
Cuts at: 0 859 1326
Size: 859 467
AlwNI CAG_nnn'CTG
Cuts at: 0 538 1326
Size: 538 788
ApaI G_GGCC'C
Cuts at: 0 74 81 141 938 1072 1326
Size: 74 7 60 797 134 254
Fragments arranged by size:
797 254 134 74 60 7
AscI GG'CGCG_CC
Cuts at: 0 126 1326
Size: 126 1200
AvaI C'yCGr_G
Cuts at: 0 73 237 249 679 1065 1098 1326
Size: 73 164 12 430 386 33 228
Fragments arranged by size:
430 386 228 164 73 33 12
BanI G'GyrC_C
Cuts at: 0 46 684 1057 1186 1326
Size: 46 638 373 129 140
Fragments arranged by size:
638 373 140 129 46
BanII G_rGCy'C
Cuts at: 0 74 81 141 190 250 386 455 774
Size: 74 7 60 49 60 136 69 319
Cuts at: 774 938 1072 1326
Size: 164 134 254
Fragments arranged by size:
319 254 164 136 134 74 69 60
60 49 7
BbeI G_GCGC'C
Cuts at: 0 50 688 1190 1326
Size: 50 638 502 136
Fragments arranged by size:
638 502 136 50
BciVI GTATCCnnnnn_n'
Cuts at: 0 1144 1326
Size: 1144 182
BglI GCCn_nnn'nGGC
Cuts at: 0 40 136 547 1326
Size: 40 96 411 779
Fragments arranged by size:
779 411 96 40
BlpI GC'TnA_GC
Cuts at: 0 1107 1115 1326
Size: 1107 8 211
Fragments arranged by size:
1107 211 8
Bme1580I G_kGCm'C
Cuts at: 0 74 81 141 370 938 1062 1072 1326
Size: 74 7 60 229 568 124 10 254
Fragments arranged by size:
568 254 229 124 74 60 10 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 932 1326
Size: 932 394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 705 1326
Size: 405 300 621
Fragments arranged by size:
621 405 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 427 863 1159 1282 1326
Size: 202 225 436 296 123 44
Fragments arranged by size:
436 296 225 202 123 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 162 877 961 1326
Size: 162 715 84 365
Fragments arranged by size:
715 365 162 84
BsaHI Gr'CG_yC
Cuts at: 0 47 685 1187 1326
Size: 47 638 502 139
Fragments arranged by size:
638 502 139 47
BsaWI w'CCGG_w
Cuts at: 0 921 1326
Size: 921 405
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 861 891 1326
Size: 861 30 435
Fragments arranged by size:
861 435 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 831 1326
Size: 831 495
BseYI C'CCAG_C
Cuts at: 0 140 454 562 1326
Size: 140 314 108 764
Fragments arranged by size:
764 314 140 108
BsiEI CG_ry'CG
Cuts at: 0 68 1326
Size: 68 1258
BsiHKAI G_wGCw'C
Cuts at: 0 386 823 1157 1326
Size: 386 437 334 169
Fragments arranged by size:
437 386 334 169
BsiWI C'GTAC_G
Cuts at: 0 621 1326
Size: 621 705
BsmBI CGTCTCn'nnnn_
Cuts at: 0 480 1326
Size: 480 846
Bsp1286I G_dGCh'C
Cuts at: 0 74 81 141 190 250 370 386 455
Size: 74 7 60 49 60 120 16 69
Cuts at: 455 774 823 938 1062 1072 1157 1326
Size: 319 49 115 124 10 85 169
Fragments arranged by size:
319 169 124 120 115 85 74 69
60 60 49 49 16 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 339 1326
Size: 339 987
BsrBI CCG'CTC
Cuts at: 0 1143 1326
Size: 1143 183
BsrFI r'CCGG_y
Cuts at: 0 43 783 1326
Size: 43 740 543
Fragments arranged by size:
740 543 43
BssHII G'CGCG_C
Cuts at: 0 126 1326
Size: 126 1200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 486 861 996 1326
Size: 426 60 375 135 330
Fragments arranged by size:
426 375 330 135 60
BtgI C'CryG_G
Cuts at: 0 36 62 81 131 173 485 701 1326
Size: 36 26 19 50 42 312 216 625
Fragments arranged by size:
625 312 216 50 42 36 26 19
BtsI GCAGTG_nn'
Cuts at: 0 159 494 596 839 1045 1213 1326
Size: 159 335 102 243 206 168 113
Fragments arranged by size:
335 243 206 168 159 113 102
EaeI y'GGCC_r
Cuts at: 0 65 527 548 590 763 785 1019 1326
Size: 65 462 21 42 173 22 234 307
Fragments arranged by size:
462 307 234 173 65 42 22 21
EagI C'GGCC_G
Cuts at: 0 65 1326
Size: 65 1261
EarI CTCTTCn'nnn_
Cuts at: 0 374 443 959 1123 1326
Size: 374 69 516 164 203
Fragments arranged by size:
516 374 203 164 69
EcoICRI GAG'CTC
Cuts at: 0 384 1326
Size: 384 942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 279 427 863 1159 1282 1326
Size: 202 77 148 436 296 123 44
Fragments arranged by size:
436 296 202 148 123 77 44
EcoO109I rG'GnC_Cy
Cuts at: 0 77 137 516 726 934 1069 1326
Size: 77 60 379 210 208 135 257
Fragments arranged by size:
379 257 210 208 135 77 60
FseI GG_CCGG'CC
Cuts at: 0 787 1326
Size: 787 539
HaeII r_GCGC'y
Cuts at: 0 50 147 399 688 1190 1326
Size: 50 97 252 289 502 136
Fragments arranged by size:
502 289 252 136 97 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 220 252 253 285 861 893
Size: 187 32 1 32 1 32 576 32
Cuts at: 893 1326
Size: 433
Fragments arranged by size:
576 433 187 32 32 32 32 1
1
KasI G'GCGC_C
Cuts at: 0 46 684 1186 1326
Size: 46 638 502 140
Fragments arranged by size:
638 502 140 46
MscI TGG'CCA
Cuts at: 0 529 550 1326
Size: 529 21 776
Fragments arranged by size:
776 529 21
MslI CAynn'nnrTG
Cuts at: 0 484 859 1326
Size: 484 375 467
Fragments arranged by size:
484 467 375
MspA1I CmG'CkG
Cuts at: 0 64 83 102 133 1111 1119 1326
Size: 64 19 19 31 978 8 207
Fragments arranged by size:
978 207 64 31 19 19 8
NaeI GCC'GGC
Cuts at: 0 45 785 1326
Size: 45 740 541
Fragments arranged by size:
740 541 45
NarI GG'CG_CC
Cuts at: 0 47 685 1187 1326
Size: 47 638 502 139
Fragments arranged by size:
638 502 139 47
NcoI C'CATG_G
Cuts at: 0 485 1326
Size: 485 841
NgoMIV G'CCGG_C
Cuts at: 0 43 783 1326
Size: 43 740 543
Fragments arranged by size:
740 543 43
PasI CC'CwG_GG
Cuts at: 0 831 1326
Size: 831 495
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 1326
Size: 363 963
PfoI T'CCnGG_A
Cuts at: 0 443 902 1079 1136 1326
Size: 443 459 177 57 190
Fragments arranged by size:
459 443 190 177 57
PpuMI rG'GwC_Cy
Cuts at: 0 516 1326
Size: 516 810
PshAI GACnn'nnGTC
Cuts at: 0 439 1326
Size: 439 887
PsiI TTA'TAA
Cuts at: 0 607 1326
Size: 607 719
PspOMI G'GGCC_C
Cuts at: 0 70 77 137 934 1068 1326
Size: 70 7 60 797 134 258
Fragments arranged by size:
797 258 134 70 60 7
PstI C_TGCA'G
Cuts at: 0 576 1326
Size: 576 750
PvuII CAG'CTG
Cuts at: 0 1111 1119 1326
Size: 1111 8 207
Fragments arranged by size:
1111 207 8
SacI G_AGCT'C
Cuts at: 0 386 1326
Size: 386 940
SacII CC_GC'GG
Cuts at: 0 65 84 134 1326
Size: 65 19 50 1192
Fragments arranged by size:
1192 65 50 19
SanDI GG'GwC_CC
Cuts at: 0 516 1326
Size: 516 810
SapI GCTCTTCn'nnn_
Cuts at: 0 374 443 1326
Size: 374 69 883
Fragments arranged by size:
883 374 69
SfcI C'TryA_G
Cuts at: 0 572 1326
Size: 572 754
SfoI GGC'GCC
Cuts at: 0 48 686 1188 1326
Size: 48 638 502 138
Fragments arranged by size:
638 502 138 48
SmaI CCC'GGG
Cuts at: 0 75 1067 1326
Size: 75 992 259
Fragments arranged by size:
992 259 75
SrfI GCCC'GGGC
Cuts at: 0 1067 1326
Size: 1067 259
StyI C'CwwG_G
Cuts at: 0 485 1022 1052 1326
Size: 485 537 30 274
Fragments arranged by size:
537 485 274 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 750 808 1326
Size: 750 58 518
Fragments arranged by size:
750 518 58
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 426 1326
Size: 423 3 900
Fragments arranged by size:
900 423 3
XmaI C'CCGG_G
Cuts at: 0 73 1065 1326
Size: 73 992 261
Fragments arranged by size:
992 261 73
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI AscI AvaI
BanI BanII BbeI BciVI BglI BlpI Bme1580I BmrI
BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BstXI BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FseI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PstI PvuII SacI SacII
SanDI SapI SfcI SfoI SmaI SrfI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BclI BfrBI BglII BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsgI BsmI BspEI
BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoNI EcoRI EcoRV FalI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PspXI PsrI PvuI RsrII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI