Restriction map for
pF1KB9508
(Linear) (Six-base) MAPSORT of: KIBB9508.seq Check: 9497 from: 1 to: 1029
KIBB9508.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 432 853 1029
Size: 432 421 176
Fragments arranged by size:
432 421 176
AccI GT'mk_AC
Cuts at: 0 221 395 1029
Size: 221 174 634
Fragments arranged by size:
634 221 174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 284 339 1029
Size: 43 241 55 690
Fragments arranged by size:
690 241 55 43
AleI CACnn'nnGTG
Cuts at: 0 331 1029
Size: 331 698
AlwNI CAG_nnn'CTG
Cuts at: 0 187 1029
Size: 187 842
ApaI G_GGCC'C
Cuts at: 0 245 1029
Size: 245 784
ApaLI G'TGCA_C
Cuts at: 0 130 1029
Size: 130 899
AvaI C'yCGr_G
Cuts at: 0 951 1029
Size: 951 78
BanI G'GyrC_C
Cuts at: 0 565 1029
Size: 565 464
BanII G_rGCy'C
Cuts at: 0 245 697 1029
Size: 245 452 332
Fragments arranged by size:
452 332 245
BbsI GAAGACnn'nnnn_
Cuts at: 0 48 1029
Size: 48 981
Bme1580I G_kGCm'C
Cuts at: 0 134 245 739 1029
Size: 134 111 494 290
Fragments arranged by size:
494 290 134 111
BmgBI CAC'GTC
Cuts at: 0 795 1029
Size: 795 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 358 1029
Size: 304 54 671
Fragments arranged by size:
671 304 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1029
Size: 127 902
BsaI GGTCTCn'nnnn_
Cuts at: 0 6 1029
Size: 6 1023
BsaAI yAC'GTr
Cuts at: 0 669 1029
Size: 669 360
BsaWI w'CCGG_w
Cuts at: 0 525 1029
Size: 525 504
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 1029
Size: 42 987
BseYI C'CCAG_C
Cuts at: 0 75 261 583 1029
Size: 75 186 322 446
Fragments arranged by size:
446 322 186 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1029
Size: 275 754
BsiEI CG_ry'CG
Cuts at: 0 546 1029
Size: 546 483
BsiHKAI G_wGCw'C
Cuts at: 0 134 328 1029
Size: 134 194 701
Fragments arranged by size:
701 194 134
BsmI GAATG_Cn'
Cuts at: 0 968 1029
Size: 968 61
Bsp1286I G_dGCh'C
Cuts at: 0 134 245 328 697 739 1029
Size: 134 111 83 369 42 290
Fragments arranged by size:
369 290 134 111 83 42
BspEI T'CCGG_A
Cuts at: 0 525 1029
Size: 525 504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 853 1029
Size: 432 421 176
Fragments arranged by size:
432 421 176
BsrFI r'CCGG_y
Cuts at: 0 451 869 977 1029
Size: 451 418 108 52
Fragments arranged by size:
451 418 108 52
BssHII G'CGCG_C
Cuts at: 0 601 1029
Size: 601 428
BssSI C'ACGA_G
Cuts at: 0 589 676 1029
Size: 589 87 353
Fragments arranged by size:
589 353 87
BstEII G'GTnAC_C
Cuts at: 0 447 648 840 1029
Size: 447 201 192 189
Fragments arranged by size:
447 201 192 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 837 1029
Size: 591 246 192
Fragments arranged by size:
591 246 192
Bsu36I CC'TnA_GG
Cuts at: 0 188 1029
Size: 188 841
BtgI C'CryG_G
Cuts at: 0 926 1019 1029
Size: 926 93 10
Fragments arranged by size:
926 93 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 676 810 1029
Size: 676 134 219
Fragments arranged by size:
676 219 134
BtsI GCAGTG_nn'
Cuts at: 0 233 1029
Size: 233 796
DrdI GACnn_nn'nnGTC
Cuts at: 0 550 1029
Size: 550 479
EaeI y'GGCC_r
Cuts at: 0 532 593 767 821 871 882 1022 1029
Size: 532 61 174 54 50 11 140 7
Fragments arranged by size:
532 174 140 61 54 50 11 7
EarI CTCTTCn'nnn_
Cuts at: 0 19 415 1029
Size: 19 396 614
Fragments arranged by size:
614 396 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 127 284 339 1029
Size: 43 84 157 55 690
Fragments arranged by size:
690 157 84 55 43
EcoO109I rG'GnC_Cy
Cuts at: 0 184 341 538 906 1029
Size: 184 157 197 368 123
Fragments arranged by size:
368 197 184 157 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 441 473 1029
Size: 3 32 406 32 556
Fragments arranged by size:
556 406 32 32 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1029
Size: 770 259
MscI TGG'CCA
Cuts at: 0 1024 1029
Size: 1024 5
MslI CAynn'nnrTG
Cuts at: 0 331 661 1029
Size: 331 330 368
Fragments arranged by size:
368 331 330
NaeI GCC'GGC
Cuts at: 0 871 1029
Size: 871 158
NcoI C'CATG_G
Cuts at: 0 926 1029
Size: 926 103
NgoMIV G'CCGG_C
Cuts at: 0 869 1029
Size: 869 160
PasI CC'CwG_GG
Cuts at: 0 138 181 504 899 1029
Size: 138 43 323 395 130
Fragments arranged by size:
395 323 138 130 43
PflMI CCAn_nnn'nTGG
Cuts at: 0 880 1029
Size: 880 149
PpuMI rG'GwC_Cy
Cuts at: 0 538 906 1029
Size: 538 368 123
Fragments arranged by size:
538 368 123
PspOMI G'GGCC_C
Cuts at: 0 241 1029
Size: 241 788
PstI C_TGCA'G
Cuts at: 0 963 1029
Size: 963 66
SfcI C'TryA_G
Cuts at: 0 959 1029
Size: 959 70
StyI C'CwwG_G
Cuts at: 0 926 1029
Size: 926 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 1029
Size: 151 878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 1029
Size: 460 32 537
Fragments arranged by size:
537 460 32
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI Bme1580I BmgBI BmrI BpmI BsaI
BsaAI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrFI BssHII BssSI BstEII BstXI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI
EcoO109I Hin4I MmeI MscI MslI NaeI NcoI NgoMIV
PasI PflMI PpuMI PspOMI PstI SfcI StyI TaqII
TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI BsaHI
BsaXI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI BstAPI
BstBI BstYI BstZ17I ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI