(Linear) (Six-base) MAPSORT of: KIBB9510.seq  Check: 5836  from: 1  to: 1116



KIBB9510.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110    1116 
   Size:        110    1006
 
AflII C'TTAA_G

Cuts at:      0     805    1116 
   Size:        805     311
 
AleI CACnn'nnGTG

Cuts at:      0     108    1116 
   Size:        108    1008
 
AlwNI CAG_nnn'CTG

Cuts at:      0     403     832    1116 
   Size:        403     429     284

  Fragments arranged by size: 

                 429     403     284
 
AvrII C'CTAG_G

Cuts at:      0     133    1116 
   Size:        133     983
 
BanI G'GyrC_C

Cuts at:      0    1039    1116 
   Size:       1039      77
 
BlpI GC'TnA_GC

Cuts at:      0      67     341    1116 
   Size:         67     274     775

  Fragments arranged by size: 

                 775     274      67
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1083    1116 
   Size:        665     418      33

  Fragments arranged by size: 

                 665     418      33
 
BsaAI yAC'GTr

Cuts at:      0     110     759    1116 
   Size:        110     649     357

  Fragments arranged by size: 

                 649     357     110
 
BseYI C'CCAG_C

Cuts at:      0     235    1116 
   Size:        235     881
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     402    1116 
   Size:        402     714
 
BsrDI GCAATG_nn'

Cuts at:      0     782    1116 
   Size:        782     334
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     970    1116 
   Size:        970     146
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     179    1116 
   Size:        179     937
 
BstYI r'GATC_y

Cuts at:      0     194    1116 
   Size:        194     922
 
Bsu36I CC'TnA_GG

Cuts at:      0     452    1116 
   Size:        452     664
 
BtsI GCAGTG_nn'

Cuts at:      0     377    1116 
   Size:        377     739
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     699     734    1116 
   Size:        699      35     382

  Fragments arranged by size: 

                 699     382      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     551    1116 
   Size:        551     565
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     665    1083    1116 
   Size:        110     555     418      33

  Fragments arranged by size: 

                 555     418     110      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      82    1116 
   Size:         82    1034
 
EcoO109I rG'GnC_Cy

Cuts at:      0     357    1116 
   Size:        357     759
 
HincII GTy'rAC

Cuts at:      0       5     479    1116 
   Size:          5     474     637

  Fragments arranged by size: 

                 637     474       5
 
HindIII A'AGCT_T

Cuts at:      0    1089    1116 
   Size:       1089      27
 
MslI CAynn'nnrTG

Cuts at:      0     108     761     863    1116 
   Size:        108     653     102     253

  Fragments arranged by size: 

                 653     253     108     102
 
MspA1I CmG'CkG

Cuts at:      0      10     345     403    1116 
   Size:         10     335      58     713

  Fragments arranged by size: 

                 713     335      58      10
 
PmlI CAC'GTG

Cuts at:      0     110    1116 
   Size:        110    1006
 
PpuMI rG'GwC_Cy

Cuts at:      0     357    1116 
   Size:        357     759
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     974    1006    1116 
   Size:        974      32     110

  Fragments arranged by size: 

                 974     110      32
 
PstI C_TGCA'G

Cuts at:      0      86     399    1023    1116 
   Size:         86     313     624      93

  Fragments arranged by size: 

                 624     313      93      86
 
PvuII CAG'CTG

Cuts at:      0      10     345     403    1116 
   Size:         10     335      58     713

  Fragments arranged by size: 

                 713     335      58      10
 
ScaI AGT'ACT

Cuts at:      0    1017    1116 
   Size:       1017      99
 
SexAI A'CCwGG_T

Cuts at:      0      44    1116 
   Size:         44    1072
 
SfcI C'TryA_G

Cuts at:      0      82     395    1019    1116 
   Size:         82     313     624      97

  Fragments arranged by size: 

                 624     313      97      82
 
SmlI C'TyrA_G

Cuts at:      0     805    1116 
   Size:        805     311
 
StyI C'CwwG_G

Cuts at:      0     133     482    1116 
   Size:        133     349     634

  Fragments arranged by size: 

                 634     349     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     120     249    1116 
   Size:        120     129     867

  Fragments arranged by size: 

                 867     129     120
 
TatI w'GTAC_w

Cuts at:      0     362    1015    1116 
   Size:        362     653     101

  Fragments arranged by size: 

                 653     362     101
 
Tth111I GACn'n_nGTC

Cuts at:      0     934    1116 
   Size:        934     182
 
XbaI T'CTAG_A

Cuts at:      0     492    1116 
   Size:        492     624
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     270    1116 
   Size:        270     846
 

 Enzymes that do cut:

     AcuI    AflII     AleI    AlwNI    AvrII     BanI     BlpI     BpmI
    BsaAI    BseYI    BspMI    BsrDI   BstAPI    BstXI    BstYI   Bsu36I
     BtsI    CspCI     EarI  Eco57MI    EcoNI EcoO109I   HincII  HindIII
     MslI   MspA1I     PmlI    PpuMI     PsrI     PstI    PvuII     ScaI
    SexAI     SfcI     SmlI     StyI    TaqII     TatI  Tth111I     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XhoI
     XmaI     XmnI     ZraI