Restriction map for
pF1KB9519
(Linear) (Six-base) MAPSORT of: KIBB9519.seq Check: 7516 from: 1 to: 1119
KIBB9519.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 608 1119
Size: 608 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1119
Size: 255 864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 300 1119
Size: 300 819
ApaI G_GGCC'C
Cuts at: 0 758 1119
Size: 758 361
ApaLI G'TGCA_C
Cuts at: 0 275 1119
Size: 275 844
AvaI C'yCGr_G
Cuts at: 0 703 1119
Size: 703 416
BanI G'GyrC_C
Cuts at: 0 487 666 1084 1119
Size: 487 179 418 35
Fragments arranged by size:
487 418 179 35
BanII G_rGCy'C
Cuts at: 0 758 1119
Size: 758 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 858 892 1119
Size: 858 34 227
Fragments arranged by size:
858 227 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 790 1119
Size: 790 329
BglI GCCn_nnn'nGGC
Cuts at: 0 304 1119
Size: 304 815
Bme1580I G_kGCm'C
Cuts at: 0 156 279 490 758 953 1078 1089 1119
Size: 156 123 211 268 195 125 11 30
Fragments arranged by size:
268 211 195 156 125 123 30 11
BmgBI CAC'GTC
Cuts at: 0 483 1119
Size: 483 636
BmrI ACTGGGnnnn_n'
Cuts at: 0 826 1119
Size: 826 293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 56 1119
Size: 56 1063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1024 1119
Size: 127 897 95
Fragments arranged by size:
897 127 95
BsaWI w'CCGG_w
Cuts at: 0 13 1119
Size: 13 1106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 1119
Size: 502 30 587
Fragments arranged by size:
587 502 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 523 613 880 1119
Size: 40 483 90 267 239
Fragments arranged by size:
483 267 239 90 40
BseYI C'CCAG_C
Cuts at: 0 84 558 774 849 1070 1119
Size: 84 474 216 75 221 49
Fragments arranged by size:
474 221 216 84 75 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 679 1119
Size: 142 537 440
Fragments arranged by size:
537 440 142
BsiHKAI G_wGCw'C
Cuts at: 0 143 279 314 587 1119
Size: 143 136 35 273 532
Fragments arranged by size:
532 273 143 136 35
Bsp1286I G_dGCh'C
Cuts at: 0 143 156 279 314 490 587 758 953
Size: 143 13 123 35 176 97 171 195
Cuts at: 953 1078 1089 1119
Size: 125 11 30
Fragments arranged by size:
195 176 171 143 125 123 97 35
30 13 11
BsrBI CCG'CTC
Cuts at: 0 35 1119
Size: 35 1084
BsrFI r'CCGG_y
Cuts at: 0 712 739 806 1119
Size: 712 27 67 313
Fragments arranged by size:
712 313 67 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 1119
Size: 666 453
BstEII G'GTnAC_C
Cuts at: 0 896 1119
Size: 896 223
Bsu36I CC'TnA_GG
Cuts at: 0 23 1051 1119
Size: 23 1028 68
Fragments arranged by size:
1028 68 23
BtgI C'CryG_G
Cuts at: 0 252 458 696 750 1119
Size: 252 206 238 54 369
Fragments arranged by size:
369 252 238 206 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 517 1119
Size: 357 160 602
Fragments arranged by size:
602 357 160
BtsI GCAGTG_nn'
Cuts at: 0 874 968 1119
Size: 874 94 151
Fragments arranged by size:
874 151 94
EaeI y'GGCC_r
Cuts at: 0 2 197 305 368 455 714 747 965
Size: 2 195 108 63 87 259 33 218
Cuts at: 965 1119
Size: 154
Fragments arranged by size:
259 218 195 154 108 87 63 33
2
EarI CTCTTCn'nnn_
Cuts at: 0 264 631 1119
Size: 264 367 488
Fragments arranged by size:
488 367 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1119
Size: 255 864
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 1119
Size: 160 959
EcoO109I rG'GnC_Cy
Cuts at: 0 1035 1119
Size: 1035 84
HaeII r_GCGC'y
Cuts at: 0 41 1119
Size: 41 1078
HincII GTy'rAC
Cuts at: 0 1005 1119
Size: 1005 114
MfeI C'AATT_G
Cuts at: 0 1010 1119
Size: 1010 109
MscI TGG'CCA
Cuts at: 0 4 370 457 967 1119
Size: 4 366 87 510 152
Fragments arranged by size:
510 366 152 87 4
MslI CAynn'nnrTG
Cuts at: 0 433 571 1119
Size: 433 138 548
Fragments arranged by size:
548 433 138
MspA1I CmG'CkG
Cuts at: 0 284 849 1119
Size: 284 565 270
Fragments arranged by size:
565 284 270
NaeI GCC'GGC
Cuts at: 0 741 808 1119
Size: 741 67 311
Fragments arranged by size:
741 311 67
NcoI C'CATG_G
Cuts at: 0 458 696 1119
Size: 458 238 423
Fragments arranged by size:
458 423 238
NgoMIV G'CCGG_C
Cuts at: 0 739 806 1119
Size: 739 67 313
Fragments arranged by size:
739 313 67
NspI r_CATG'y
Cuts at: 0 419 799 861 1119
Size: 419 380 62 258
Fragments arranged by size:
419 380 258 62
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1119
Size: 1035 84
PspOMI G'GGCC_C
Cuts at: 0 754 1119
Size: 754 365
PvuII CAG'CTG
Cuts at: 0 284 849 1119
Size: 284 565 270
Fragments arranged by size:
565 284 270
SapI GCTCTTCn'nnn_
Cuts at: 0 264 1119
Size: 264 855
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 304 1119
Size: 304 815
SmaI CCC'GGG
Cuts at: 0 705 1119
Size: 705 414
SmlI C'TyrA_G
Cuts at: 0 142 1039 1119
Size: 142 897 80
Fragments arranged by size:
897 142 80
SphI G_CATG'C
Cuts at: 0 799 1119
Size: 799 320
StyI C'CwwG_G
Cuts at: 0 458 696 1119
Size: 458 238 423
Fragments arranged by size:
458 423 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 258 290 502 534 1119
Size: 258 32 212 32 585
Fragments arranged by size:
585 258 212 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 447 829 1119
Size: 447 382 290
Fragments arranged by size:
447 382 290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 1119
Size: 555 564
XmaI C'CCGG_G
Cuts at: 0 703 1119
Size: 703 416
Enzymes that do cut:
AccI AcuI AlfI ApaI ApaLI AvaI BanI BanII
BcgI BciVI BglI Bme1580I BmgBI BmrI BplI BpuEI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI
BsrFI BstAPI BstEII Bsu36I BtgI BtgZI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I HaeII HincII MfeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PpuMI PspOMI
PvuII SapI SfiI SmaI SmlI SphI StyI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BclI BfrBI
BglII BlpI BmtI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MluI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI XbaI XhoI XmnI
ZraI