Restriction map for
pF1KB9521
(Linear) (Six-base) MAPSORT of: KIBB9521.seq Check: 5911 from: 1 to: 1764
KIBB9521.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 319 1764
Size: 319 1445
Acc65I G'GTAC_C
Cuts at: 0 700 720 1764
Size: 700 20 1044
Fragments arranged by size:
1044 700 20
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1433 1764
Size: 531 902 331
Fragments arranged by size:
902 531 331
AfeI AGC'GCT
Cuts at: 0 484 985 1764
Size: 484 501 779
Fragments arranged by size:
779 501 484
AleI CACnn'nnGTG
Cuts at: 0 853 1764
Size: 853 911
AlwNI CAG_nnn'CTG
Cuts at: 0 402 615 951 1764
Size: 402 213 336 813
Fragments arranged by size:
813 402 336 213
ApaI G_GGCC'C
Cuts at: 0 624 1344 1732 1764
Size: 624 720 388 32
Fragments arranged by size:
720 624 388 32
AvaI C'yCGr_G
Cuts at: 0 5 746 1268 1345 1764
Size: 5 741 522 77 419
Fragments arranged by size:
741 522 419 77 5
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 246 279 1764
Size: 246 33 1485
Fragments arranged by size:
1485 246 33
BamHI G'GATC_C
Cuts at: 0 1720 1764
Size: 1720 44
BanI G'GyrC_C
Cuts at: 0 700 720 855 900 1764
Size: 700 20 135 45 864
Fragments arranged by size:
864 700 135 45 20
BanII G_rGCy'C
Cuts at: 0 74 624 639 1344 1732 1764
Size: 74 550 15 705 388 32
Fragments arranged by size:
705 550 388 74 32 15
BbeI G_GCGC'C
Cuts at: 0 904 1764
Size: 904 860
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 1764
Size: 267 1497
BbvCI CC'TCA_GC
Cuts at: 0 106 379 1764
Size: 106 273 1385
Fragments arranged by size:
1385 273 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 258 292 1555 1589 1764
Size: 208 34 16 34 1263 34 175
Fragments arranged by size:
1263 208 175 34 34 34 16
BglI GCCn_nnn'nGGC
Cuts at: 0 1170 1764
Size: 1170 594
Bme1580I G_kGCm'C
Cuts at: 0 172 197 624 860 1344 1568 1732 1764
Size: 172 25 427 236 484 224 164 32
Fragments arranged by size:
484 427 236 224 172 164 32 25
BmgBI CAC'GTC
Cuts at: 0 999 1764
Size: 999 765
BmrI ACTGGGnnnn_n'
Cuts at: 0 1600 1764
Size: 1600 164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 48 1764
Size: 48 1716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1764
Size: 163 1601
Bpu10I CC'TnA_GC
Cuts at: 0 106 379 1764
Size: 106 273 1385
Fragments arranged by size:
1385 273 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1213 1764
Size: 871 342 551
Fragments arranged by size:
871 551 342
BsaHI Gr'CG_yC
Cuts at: 0 901 1230 1764
Size: 901 329 534
Fragments arranged by size:
901 534 329
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 1054 1178 1408 1522 1562 1764
Size: 40 1014 124 230 114 40 202
Fragments arranged by size:
1014 230 202 124 114 40 40
BseYI C'CCAG_C
Cuts at: 0 1326 1751 1764
Size: 1326 425 13
Fragments arranged by size:
1326 425 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1273 1764
Size: 238 1035 491
Fragments arranged by size:
1035 491 238
BsiEI CG_ry'CG
Cuts at: 0 323 1764
Size: 323 1441
BsiHKAI G_wGCw'C
Cuts at: 0 937 1764
Size: 937 827
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1665 1764
Size: 1665 99
Bsp1286I G_dGCh'C
Cuts at: 0 74 172 197 624 639 860 937 1344
Size: 74 98 25 427 15 221 77 407
Cuts at: 1344 1568 1732 1764
Size: 224 164 32
Fragments arranged by size:
427 407 224 221 164 98 77 74
32 25 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 319 792 1764
Size: 319 473 972
Fragments arranged by size:
972 473 319
BsrDI GCAATG_nn'
Cuts at: 0 1024 1764
Size: 1024 740
BstAPI GCAn_nnn'nTGC
Cuts at: 0 402 406 1764
Size: 402 4 1358
Fragments arranged by size:
1358 402 4
BstEII G'GTnAC_C
Cuts at: 0 804 1764
Size: 804 960
BstXI CCAn_nnnn'nTGG
Cuts at: 0 735 867 1764
Size: 735 132 897
Fragments arranged by size:
897 735 132
BstYI r'GATC_y
Cuts at: 0 1720 1764
Size: 1720 44
BtgI C'CryG_G
Cuts at: 0 212 495 938 1483 1764
Size: 212 283 443 545 281
Fragments arranged by size:
545 443 283 281 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 325 1764
Size: 325 1439
BtsI GCAGTG_nn'
Cuts at: 0 400 448 1764
Size: 400 48 1316
Fragments arranged by size:
1316 400 48
ClaI AT'CG_AT
Cuts at: 0 1526 1764
Size: 1526 238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 663 698 1764
Size: 663 35 1066
Fragments arranged by size:
1066 663 35
DraIII CAC_nnn'GTG
Cuts at: 0 1010 1764
Size: 1010 754
DrdI GACnn_nn'nnGTC
Cuts at: 0 1318 1764
Size: 1318 446
EaeI y'GGCC_r
Cuts at: 0 86 116 305 593 706 1025 1427 1585
Size: 86 30 189 288 113 319 402 158
Cuts at: 1585 1764
Size: 179
Fragments arranged by size:
402 319 288 189 179 158 113 86
30
EarI CTCTTCn'nnn_
Cuts at: 0 1186 1400 1764
Size: 1186 214 364
Fragments arranged by size:
1186 364 214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 531 1433 1764
Size: 163 368 902 331
Fragments arranged by size:
902 368 331 163
EcoO109I rG'GnC_Cy
Cuts at: 0 692 1140 1215 1320 1341 1351 1477 1489
Size: 692 448 75 105 21 10 126 12
Cuts at: 1489 1622 1728 1764
Size: 133 106 36
Fragments arranged by size:
692 448 133 126 106 105 75 36
21 12 10
FspI TGC'GCA
Cuts at: 0 1003 1764
Size: 1003 761
FspAI rTGC'GCAy
Cuts at: 0 1003 1764
Size: 1003 761
HaeII r_GCGC'y
Cuts at: 0 82 304 486 824 904 987 1764
Size: 82 222 182 338 80 83 777
Fragments arranged by size:
777 338 222 182 83 82 80
HincII GTy'rAC
Cuts at: 0 369 1764
Size: 369 1395
KasI G'GCGC_C
Cuts at: 0 900 1764
Size: 900 864
KpnI G_GTAC'C
Cuts at: 0 704 724 1764
Size: 704 20 1040
Fragments arranged by size:
1040 704 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 266 872 1364 1573 1623 1764
Size: 68 198 606 492 209 50 141
Fragments arranged by size:
606 492 209 198 141 68 50
MscI TGG'CCA
Cuts at: 0 88 307 595 1027 1429 1764
Size: 88 219 288 432 402 335
Fragments arranged by size:
432 402 335 288 219 88
MslI CAynn'nnrTG
Cuts at: 0 181 349 424 733 853 865 1764
Size: 181 168 75 309 120 12 899
Fragments arranged by size:
899 309 181 168 120 75 12
MspA1I CmG'CkG
Cuts at: 0 190 222 497 1755 1764
Size: 190 32 275 1258 9
Fragments arranged by size:
1258 275 190 32 9
NarI GG'CG_CC
Cuts at: 0 901 1764
Size: 901 863
NcoI C'CATG_G
Cuts at: 0 938 1764
Size: 938 826
NspI r_CATG'y
Cuts at: 0 348 409 1179 1764
Size: 348 61 770 585
Fragments arranged by size:
770 585 348 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 95 464 1446 1764
Size: 95 369 982 318
Fragments arranged by size:
982 369 318 95
PfoI T'CCnGG_A
Cuts at: 0 1502 1764
Size: 1502 262
PpuMI rG'GwC_Cy
Cuts at: 0 1140 1215 1320 1351 1477 1489 1622 1764
Size: 1140 75 105 31 126 12 133 142
Fragments arranged by size:
1140 142 133 126 105 75 31 12
PspOMI G'GGCC_C
Cuts at: 0 620 1340 1728 1764
Size: 620 720 388 36
Fragments arranged by size:
720 620 388 36
PstI C_TGCA'G
Cuts at: 0 803 1764
Size: 803 961
PvuII CAG'CTG
Cuts at: 0 190 222 1755 1764
Size: 190 32 1533 9
Fragments arranged by size:
1533 190 32 9
SacII CC_GC'GG
Cuts at: 0 498 1764
Size: 498 1266
SanDI GG'GwC_CC
Cuts at: 0 1140 1477 1622 1764
Size: 1140 337 145 142
Fragments arranged by size:
1140 337 145 142
SapI GCTCTTCn'nnn_
Cuts at: 0 1186 1764
Size: 1186 578
SbfI CC_TGCA'GG
Cuts at: 0 803 1764
Size: 803 961
SfcI C'TryA_G
Cuts at: 0 799 1764
Size: 799 965
SfoI GGC'GCC
Cuts at: 0 902 1764
Size: 902 862
SmlI C'TyrA_G
Cuts at: 0 886 1192 1764
Size: 886 306 572
Fragments arranged by size:
886 572 306
SphI G_CATG'C
Cuts at: 0 409 1764
Size: 409 1355
StuI AGG'CCT
Cuts at: 0 31 1764
Size: 31 1733
StyI C'CwwG_G
Cuts at: 0 695 938 1617 1764
Size: 695 243 679 147
Fragments arranged by size:
695 679 243 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 1764
Size: 210 1554
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlwNI ApaI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EarI Eco57MI
EcoO109I FspI FspAI HaeII HincII KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NspI PflMI PfoI
PpuMI PspOMI PstI PvuII SacII SanDI SapI SbfI
SfcI SfoI SmlI SphI StuI StyI XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BciVI
BclI BfrBI BglII BlpI BmtI BsaI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I DraI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I
HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI