Restriction map for
pF1KB9540
(Linear) (Six-base) MAPSORT of: KIBB9540.seq Check: 7198 from: 1 to: 1053
KIBB9540.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
Acc65I G'GTAC_C
Cuts at: 0 881 1053
Size: 881 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 587 626 1053
Size: 101 486 39 427
Fragments arranged by size:
486 427 101 39
AhdI GACnn_n'nnGTC
Cuts at: 0 48 1053
Size: 48 1005
AlwNI CAG_nnn'CTG
Cuts at: 0 999 1053
Size: 999 54
AvrII C'CTAG_G
Cuts at: 0 90 1053
Size: 90 963
BanI G'GyrC_C
Cuts at: 0 881 1053
Size: 881 172
BanII G_rGCy'C
Cuts at: 0 172 673 721 1053
Size: 172 501 48 332
Fragments arranged by size:
501 332 172 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 638 1053
Size: 638 415
BbvCI CC'TCA_GC
Cuts at: 0 695 1053
Size: 695 358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 305 442 476 1053
Size: 271 34 137 34 577
Fragments arranged by size:
577 271 137 34 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 133 503 728 812 1053
Size: 132 1 370 225 84 241
Fragments arranged by size:
370 241 225 132 84 1
Bpu10I CC'TnA_GC
Cuts at: 0 19 161 695 1053
Size: 19 142 534 358
Fragments arranged by size:
534 358 142 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 695 1053
Size: 100 595 358
Fragments arranged by size:
595 358 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 1053
Size: 979 30 44
Fragments arranged by size:
979 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 162 683 1053
Size: 162 521 370
Fragments arranged by size:
521 370 162
BseYI C'CCAG_C
Cuts at: 0 543 1053
Size: 543 510
Bsp1286I G_dGCh'C
Cuts at: 0 172 673 721 1053
Size: 172 501 48 332
Fragments arranged by size:
501 332 172 48
BstYI r'GATC_y
Cuts at: 0 989 1053
Size: 989 64
BtsI GCAGTG_nn'
Cuts at: 0 555 590 1053
Size: 555 35 463
Fragments arranged by size:
555 463 35
EaeI y'GGCC_r
Cuts at: 0 119 423 980 1053
Size: 119 304 557 73
Fragments arranged by size:
557 304 119 73
EarI CTCTTCn'nnn_
Cuts at: 0 491 889 1010 1053
Size: 491 398 121 43
Fragments arranged by size:
491 398 121 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 916 985 1053
Size: 916 69 68
Fragments arranged by size:
916 69 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 132 133 503 587 626 728 812
Size: 101 31 1 370 84 39 102 84
Cuts at: 812 1053
Size: 241
Fragments arranged by size:
370 241 102 101 84 84 39 31
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 711 982 1053
Size: 379 332 271 71
Fragments arranged by size:
379 332 271 71
EcoO109I rG'GnC_Cy
Cuts at: 0 504 1047 1053
Size: 504 543 6
Fragments arranged by size:
543 504 6
EcoRV GAT'ATC
Cuts at: 0 561 1053
Size: 561 492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 220 252 1053
Size: 34 32 154 32 801
Fragments arranged by size:
801 154 34 32 32
KpnI G_GTAC'C
Cuts at: 0 885 1053
Size: 885 168
MfeI C'AATT_G
Cuts at: 0 419 1053
Size: 419 634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 326 1053
Size: 236 90 727
Fragments arranged by size:
727 236 90
MscI TGG'CCA
Cuts at: 0 121 425 982 1053
Size: 121 304 557 71
Fragments arranged by size:
557 304 121 71
MslI CAynn'nnrTG
Cuts at: 0 664 1053
Size: 664 389
PasI CC'CwG_GG
Cuts at: 0 499 1053
Size: 499 554
PfoI T'CCnGG_A
Cuts at: 0 238 380 789 1053
Size: 238 142 409 264
Fragments arranged by size:
409 264 238 142
PpuMI rG'GwC_Cy
Cuts at: 0 504 1047 1053
Size: 504 543 6
Fragments arranged by size:
543 504 6
SanDI GG'GwC_CC
Cuts at: 0 1047 1053
Size: 1047 6
SexAI A'CCwGG_T
Cuts at: 0 455 1053
Size: 455 598
StyI C'CwwG_G
Cuts at: 0 90 983 1053
Size: 90 893 70
Fragments arranged by size:
893 90 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 1053
Size: 19 1034
XmnI GAAnn'nnTTC
Cuts at: 0 277 1053
Size: 277 776
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI AvrII BanI BanII BbsI
BbvCI BcgI BpmI Bpu10I BsaI BsaXI BseRI BseYI
Bsp1286I BstYI BtsI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV Hin4I KpnI MfeI MmeI MscI MslI
PasI PfoI PpuMI SanDI SexAI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BbeI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI