Restriction map for
pF1KB9542
(Linear) (Six-base) MAPSORT of: KIBB9542.seq Check: 8857 from: 1 to: 870
KIBB9542.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 274 668 794 870
Size: 64 210 394 126 76
Fragments arranged by size:
394 210 126 76 64
AfeI AGC'GCT
Cuts at: 0 518 870
Size: 518 352
AflIII A'CryG_T
Cuts at: 0 133 498 870
Size: 133 365 372
Fragments arranged by size:
372 365 133
AgeI A'CCGG_T
Cuts at: 0 791 870
Size: 791 79
AlwNI CAG_nnn'CTG
Cuts at: 0 645 870
Size: 645 225
AseI AT'TA_AT
Cuts at: 0 152 205 870
Size: 152 53 665
Fragments arranged by size:
665 152 53
BanI G'GyrC_C
Cuts at: 0 763 870
Size: 763 107
BglI GCCn_nnn'nGGC
Cuts at: 0 399 870
Size: 399 471
BglII A'GATC_T
Cuts at: 0 474 870
Size: 474 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 870
Size: 730 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 823 870
Size: 499 324 47
Fragments arranged by size:
499 324 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 17 625 870
Size: 17 608 245
Fragments arranged by size:
608 245 17
BsaWI w'CCGG_w
Cuts at: 0 791 870
Size: 791 79
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 841 870
Size: 841 29
BseYI C'CCAG_C
Cuts at: 0 28 159 347 714 870
Size: 28 131 188 367 156
Fragments arranged by size:
367 188 156 131 28
BsiHKAI G_wGCw'C
Cuts at: 0 783 870
Size: 783 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 12 870
Size: 12 858
Bsp1286I G_dGCh'C
Cuts at: 0 783 870
Size: 783 87
BsrFI r'CCGG_y
Cuts at: 0 102 791 870
Size: 102 689 79
Fragments arranged by size:
689 102 79
BssSI C'ACGA_G
Cuts at: 0 823 870
Size: 823 47
BstEII G'GTnAC_C
Cuts at: 0 634 870
Size: 634 236
BstXI CCAn_nnnn'nTGG
Cuts at: 0 532 674 870
Size: 532 142 196
Fragments arranged by size:
532 196 142
BstYI r'GATC_y
Cuts at: 0 474 870
Size: 474 396
BtgI C'CryG_G
Cuts at: 0 615 870
Size: 615 255
DraIII CAC_nnn'GTG
Cuts at: 0 657 870
Size: 657 213
EarI CTCTTCn'nnn_
Cuts at: 0 301 870
Size: 301 569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 457 870
Size: 457 413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 274 668 730 794 870
Size: 64 210 394 62 64 76
Fragments arranged by size:
394 210 76 64 64 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 416 870
Size: 416 454
EcoO109I rG'GnC_Cy
Cuts at: 0 689 870
Size: 689 181
HaeII r_GCGC'y
Cuts at: 0 520 870
Size: 520 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 870
Size: 44 32 794
Fragments arranged by size:
794 44 32
HindIII A'AGCT_T
Cuts at: 0 232 870
Size: 232 638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 575 870
Size: 75 500 295
Fragments arranged by size:
500 295 75
MslI CAynn'nnrTG
Cuts at: 0 121 276 870
Size: 121 155 594
Fragments arranged by size:
594 155 121
NaeI GCC'GGC
Cuts at: 0 104 870
Size: 104 766
NcoI C'CATG_G
Cuts at: 0 615 870
Size: 615 255
NgoMIV G'CCGG_C
Cuts at: 0 102 870
Size: 102 768
NspI r_CATG'y
Cuts at: 0 137 502 870
Size: 137 365 368
Fragments arranged by size:
368 365 137
PciI A'CATG_T
Cuts at: 0 133 498 870
Size: 133 365 372
Fragments arranged by size:
372 365 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 119 657 870
Size: 119 538 213
Fragments arranged by size:
538 213 119
PpuMI rG'GwC_Cy
Cuts at: 0 689 870
Size: 689 181
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 426 458 870
Size: 426 32 412
Fragments arranged by size:
426 412 32
PstI C_TGCA'G
Cuts at: 0 417 870
Size: 417 453
SanDI GG'GwC_CC
Cuts at: 0 689 870
Size: 689 181
SbfI CC_TGCA'GG
Cuts at: 0 417 870
Size: 417 453
SfcI C'TryA_G
Cuts at: 0 413 870
Size: 413 457
SmlI C'TyrA_G
Cuts at: 0 478 838 870
Size: 478 360 32
Fragments arranged by size:
478 360 32
StuI AGG'CCT
Cuts at: 0 411 870
Size: 411 459
StyI C'CwwG_G
Cuts at: 0 615 870
Size: 615 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 361 526 870
Size: 20 341 165 344
Fragments arranged by size:
344 341 165 20
TatI w'GTAC_w
Cuts at: 0 237 834 870
Size: 237 597 36
Fragments arranged by size:
597 237 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 870
Size: 120 750
Enzymes that do cut:
AcuI AfeI AflIII AgeI AlwNI AseI BanI BglI
BglII BpmI BpuEI BsaI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BsrFI BssSI BstEII BstXI BstYI BtgI
DraIII EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HindIII MmeI MslI NaeI NcoI NgoMIV NspI PciI
PflMI PpuMI PsrI PstI SanDI SbfI SfcI SmlI
StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MscI MspA1I NarI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI