(Linear) (Six-base) MAPSORT of: KIBB9547.seq  Check: 2410  from: 1  to: 330



KIBB9547.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     193     330 
   Size:        193     137
 
AleI CACnn'nnGTG

Cuts at:      0     124     330 
   Size:        124     206
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     142     330 
   Size:        142     188
 
ApaLI G'TGCA_C

Cuts at:      0     222     330 
   Size:        222     108
 
BanII G_rGCy'C

Cuts at:      0     280     330 
   Size:        280      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66     330 
   Size:         66     264
 
Bme1580I G_kGCm'C

Cuts at:      0     226     330 
   Size:        226     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     201     330 
   Size:        201     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     330 
   Size:        102     228
 
BsiHKAI G_wGCw'C

Cuts at:      0     226     280     330 
   Size:        226      54      50

  Fragments arranged by size: 

                 226      54      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     226     280     330 
   Size:        226      54      50

  Fragments arranged by size: 

                 226      54      50
 
BstYI r'GATC_y

Cuts at:      0     154     330 
   Size:        154     176
 
EarI CTCTTCn'nnn_

Cuts at:      0     298     319     330 
   Size:        298      21      11

  Fragments arranged by size: 

                 298      21      11
 
EcoICRI GAG'CTC

Cuts at:      0     278     330 
   Size:        278      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     187     330 
   Size:        187     143
 
FspI TGC'GCA

Cuts at:      0     130     330 
   Size:        130     200
 
FspAI rTGC'GCAy

Cuts at:      0     130     330 
   Size:        130     200
 
HaeII r_GCGC'y

Cuts at:      0     171     330 
   Size:        171     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     179     211     252     284     330 
   Size:        179      32      41      32      46

  Fragments arranged by size: 

                 179      46      41      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     124     330 
   Size:        124     206
 
NdeI CA'TA_TG

Cuts at:      0     133     330 
   Size:        133     197
 
PfoI T'CCnGG_A

Cuts at:      0     230     330 
   Size:        230     100
 
PpuMI rG'GwC_Cy

Cuts at:      0     187     330 
   Size:        187     143
 
SacI G_AGCT'C

Cuts at:      0     280     330 
   Size:        280      50
 

 Enzymes that do cut:

     AhdI     AleI     AlfI    ApaLI    BanII     BbsI Bme1580I     BsaI
     BsgI  BsiHKAI Bsp1286I    BstYI     EarI  EcoICRI EcoO109I     FspI
    FspAI    HaeII    Hin4I     MslI     NdeI     PfoI    PpuMI     SacI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI